http://lod.bco-dmo.org/id/dataset/661405
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2016-10-12
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Daily cell count data for low to high pCO2 acclimated E. huxleyi (E Hux Response to pCO2 project)
2016-10-11
publication
2016-10-11
revision
BCO-DMO Linked Data URI
2016-10-11
creation
http://lod.bco-dmo.org/id/dataset/661405
M. Brady Olson
Western Washington University
principalInvestigator
Brooke Love
Western Washington University
principalInvestigator
Suzanne Strom
Western Washington University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Olson, M. Brady, Love, B., Strom, S. (2016) Daily cell count data for low to high pCO2 acclimated E. huxleyi (E Hux Response to pCO2 project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2016-10-11 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/661405 [access date]
Daily cell count data for low to high pCO2 acclimated E. huxleyi Dataset Description: <p><strong>Related Datasets:</strong><br />
<a href="http://www.bco-dmo.org/dataset/661464">Grazing experiments 2 and 3: cell volume</a><br />
<a href="http://www.bco-dmo.org/dataset/661354">Grazing experiments 2 and 3: CN data</a><br />
<a href="http://www.bco-dmo.org/dataset/661421">Grazing experiments 2 and 3: ingestion</a><br />
<a href="http://www.bco-dmo.org/dataset/661388">Grazing experiments 2 and 3: pCO2</a></p>
<p><strong>Related Reference:</strong><br />
Kendall, K., Marine Microzooplankton are Indirectly Affected by Ocean Acidification Through Direct Effects on Their Phytoplankton Prey. (Masters Thesis) Western Washington University.<br />
<a href="http://cedar.wwu.edu/wwuet/448/">http://cedar.wwu.edu/wwuet/448/</a></p> Methods and Sampling: <p>The phytoplankton Emiliania huxleyi CCMP 2668 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv).&nbsp; Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients.&nbsp; Each morning of dilution the cultures were gently mixed prior to a small sample being taken for cell counts.&nbsp; Cells were counted live on a BD FACSCalibur flow cytometer and were quantified based on side scatter and red fluorescence parameters.&nbsp; Prior to counting, a measured volume of 2µm yellow-green latex bead solution (Flow Check intermediate intensity level 1 fluorescence in nanopure water) was added to each sample of E. huxleyi.&nbsp; The bead solution was counted manually with epi-fluorescence microscopy using UV light.&nbsp; Cell concentration was determined by the ratio between cells and beads counted.&nbsp; The dilution volume was then calculated to achieve a cell density that would remain close to or under 100,000 cells per ml during the next day of growth.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0961229 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0961229
completed
M. Brady Olson
Western Washington University
(360) 650-7400
1900 Shannon Point Rd.
Anacortes
WA
98221
United States
Brady.Olson@wwu.edu
pointOfContact
Brooke Love
Western Washington University
(360) 650-2894
516 High St. Mail Stop 9181
Bellingham
WA
98225
United States
brooke.love@wwu.edu
pointOfContact
Suzanne Strom
Western Washington University
360-293-2188
Shannon Point Marine Center 1900 Shannon Point Rd
Anacortes
WA
98221
USA
suzanne.strom@wwu.edu
pointOfContact
asNeeded
Unknown
treatment
culture_day
cell_count
theme
None, User defined
treatment
days
cell_concentration
featureType
BCO-DMO Standard Parameters
Flow Cytometer
instrument
BCO-DMO Standard Instruments
Lab_Olson_B
service
Deployment Activity
Bellingham, WA
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton
https://osprey.bco-dmo.org/project/517694
Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton
<p><em><strong>Description from NSF award abstract:</strong></em><br />
The calcifying Haptophyte <em>Emiliania huxleyi </em>appears to be acutely sensitive to the rising concentration of ocean pCO2. Documented responses by <em>E. huxleyi </em>to elevated pCO2 include modifications to their calcification rate and cell size, malformation of coccoliths, elevated growth rates, increased organic carbon production, lowering of PIC:POC ratios, and elevated production of the active climate gas DMS. Changes in these parameters are mechanisms known to elicit alterations in grazing behavior by microzooplankton, the oceans dominant grazer functional group. The investigators hypothesize that modifications to the physiology and biochemistry of calcifying and non-calcifying Haptophyte <em>Emiliania huxleyi </em>in response to elevated pCO2 will precipitate alterations in microzooplankton grazing dynamics. To test this hypothesis, they will conduct controlled laboratory experiments where several strains of <em>E. huxleyi</em> are grown at several CO2 concentrations. After careful characterization of the biochemical and physiological responses of the <em>E. huxleyi</em> strains to elevated pCO2, they will provide these strains as food to several ecologically-important microzooplankton and document grazing dynamics.<em> E. huxleyi</em> is an ideal organism for the study of phytoplankton and microzooplankton responses to rising anthropogenic CO2, the effects of which in the marine environment are called ocean acidification; <em>E. huxleyi </em>is biogeochemically important, is well studied, numerous strains are in culture that exhibit variation in the parameters described above, and they are readily fed upon by ecologically important microzooplankton.</p>
<p>The implications of changes in microzooplankton grazing for carbon cycling, specifically CaCO3 export, DMS production, nutrient regeneration in surface waters, and carbon transfer between trophic levels are profound, as this grazing, to a large degree, regulates all these processes. <em>E. huxleyi </em>is a model prey organism because it is one of the most biogeochemically influential global phytoplankton. It forms massive seasonal blooms, contributes significantly to marine inorganic and organic carbon cycles, is a large producer of the climatically active gas DMS, and is a source of organic matter for trophic levels both above and below itself. The planned controlled study will increase our knowledge of the mechanisms that drive patterns of change between trophic levels, thus providing a wider array of tools necessary to understand the complex nature of ocean acidification field studies, where competing variables can confound precise interpretation.</p>
E Hux Response to pCO2
largerWorkCitation
project
eng; USA
biota
oceans
Bellingham, WA
2016-10-11
0
BCO-DMO catalogue of parameters from Daily cell count data for low to high pCO2 acclimated E. huxleyi (E Hux Response to pCO2 project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/661417.rdf
Name: treatment
Units: unitless
Description: <p>pCO2 level (ambient; moderate; high) and replicate (A; B; C)</p>
http://lod.bco-dmo.org/id/dataset-parameter/661418.rdf
Name: culture_day
Units: days
Description: <p>days the cells have been in culture: count day for the morning cell count or count day post-dilution for the predicted cell count after dilution</p>
http://lod.bco-dmo.org/id/dataset-parameter/661419.rdf
Name: cell_count
Units: cells/milliliter
Description: <p>cells counted</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
3101
https://datadocs.bco-dmo.org/file/ZZZgxOEC5KxQPA/expt2_3_cell_counts_nm.csv
expt2_3_cell_counts_nm.csv
Primary data file for dataset ID 661405
download
https://osprey.bco-dmo.org/dataset/661405/data/download
download
onLine
dataset
<p>The phytoplankton Emiliania huxleyi CCMP 2668 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv).&nbsp; Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients.&nbsp; Each morning of dilution the cultures were gently mixed prior to a small sample being taken for cell counts.&nbsp; Cells were counted live on a BD FACSCalibur flow cytometer and were quantified based on side scatter and red fluorescence parameters.&nbsp; Prior to counting, a measured volume of 2µm yellow-green latex bead solution (Flow Check intermediate intensity level 1 fluorescence in nanopure water) was added to each sample of E. huxleyi.&nbsp; The bead solution was counted manually with epi-fluorescence microscopy using UV light.&nbsp; Cell concentration was determined by the ratio between cells and beads counted.&nbsp; The dilution volume was then calculated to achieve a cell density that would remain close to or under 100,000 cells per ml during the next day of growth.</p>
Specified by the Principal Investigator(s)
<p>The known bead solution standard was used to determine the actual volume that was analyzed in the flow cytometer for each sample. The events per ml was then calculated.</p>
<p><strong>BCO-DMO Processing:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- column names reformatted to comply with BCO-DMO standards<br />
- transformed table columns to rows</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: PI Supplied Instrument Description:BD FACSCalibur flow cytometer Instrument Name: Flow Cytometer Instrument Short Name:Flow Cytometer Instrument Description: Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/
Deployment: Lab_Olson_B
Lab_Olson_B
WWU
laboratory
WWU
laboratory