http://lod.bco-dmo.org/id/dataset/661421
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2016-10-12
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Microzooplankton ingestion on low to high pCO2 acclimated E. huxleyi: Favella and Oxyrrhis as grazers (E Hux Response to pCO2 project)
2016-10-18
publication
2016-10-18
revision
BCO-DMO Linked Data URI
2016-10-18
creation
http://lod.bco-dmo.org/id/dataset/661421
M. Brady Olson
Western Washington University
principalInvestigator
Brooke Love
Western Washington University
principalInvestigator
Suzanne Strom
Western Washington University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Olson, M. Brady, Love, B., Strom, S. (2016) Microzooplankton ingestion on low to high pCO2 acclimated E. huxleyi: Favella and Oxyrrhis as grazers (E Hux Response to pCO2 project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2016-10-18 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/661421 [access date]
Microzooplankton ingestion on low to high pCO2 acclimated E. huxleyi (Favella and Oxyrrhis as grazers) Dataset Description: <p>These data are unprocessed counts of the E. huxleyi cells ingested by each Favella or Oxyrrhis grazer.</p>
<p><strong>Related Datasets:</strong><br />
<a href="http://www.bco-dmo.org/dataset/661464">Grazing experiments 2 and 3: cell volume</a><br />
<a href="http://www.bco-dmo.org/dataset/661354">Grazing experiments 2 and 3: CN data</a><br />
<a href="http://www.bco-dmo.org/dataset/661405">Grazing experiments 2 and 3: daily cell counts</a><br />
<a href="http://www.bco-dmo.org/dataset/661388">Grazing experiments 2 and 3: pCO2</a></p>
<p><strong>Related Reference:</strong><br />
Kendall, K., Marine Microzooplankton are Indirectly Affected by Ocean Acidification Through Direct Effects on Their Phytoplankton Prey. (Masters Thesis) Western Washington University.<br />
<a href="http://cedar.wwu.edu/wwuet/448/">http://cedar.wwu.edu/wwuet/448/</a></p> Methods and Sampling: <p>The phytoplankton Emiliania huxleyi CCMP 2668 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv). Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients.</p>
<p>Expt. 2: On day 10, after ~ 20 generations, E. huxleyi cells from the treatments were fed to starved Favella taraikaensis cells for 15, 30 and 45 minutes. During each sampling time point, 20 mls of experiment volume was removed, fixed with glutaraldehyde and stained with DAPI. This volume was filtered onto a 20 µm pore size polycarbonate filter with, which was then put on a slide with immersion oil and kept frozen until evaluation.</p>
<p>Expt. 3: On day 10, after ~ 20 generations, E. huxleyi cells from the treatments were fed to starved Oxyrrhis marina cells for 30, 60 and 90 minutes.&nbsp;During each sampling time point, 20 mls of experiment volume was removed, fixed with glutaraldehyde and stained with DAPI. This volume was filtered onto a 10 µm pore size polycarbonate filter with, which was then put on a slide with immersion oil and kept frozen until evaluation.</p>
<p>Slides were evaluated under 1000x oil immersion using an epi-fluorescent microscope under blue-light excitation. The first 100 microzooplankton on each slide were assessed for each replicate/treatment, and individual prey cell were counted in the grazer food vacuole.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0961229 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0961229
completed
M. Brady Olson
Western Washington University
(360) 650-7400
1900 Shannon Point Rd.
Anacortes
WA
98221
United States
Brady.Olson@wwu.edu
pointOfContact
Brooke Love
Western Washington University
(360) 650-2894
516 High St. Mail Stop 9181
Bellingham
WA
98225
United States
brooke.love@wwu.edu
pointOfContact
Suzanne Strom
Western Washington University
360-293-2188
Shannon Point Marine Center 1900 Shannon Point Rd
Anacortes
WA
98221
USA
suzanne.strom@wwu.edu
pointOfContact
asNeeded
Unknown
expt
grazer_analyzed
treatment_rep_min
cells_ingested
theme
None, User defined
experiment id
sample identification
treatment
count
featureType
BCO-DMO Standard Parameters
Fluorescence Microscope
instrument
BCO-DMO Standard Instruments
Lab_Olson_B
service
Deployment Activity
Bellingham, WA
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton
https://osprey.bco-dmo.org/project/517694
Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton
<p><em><strong>Description from NSF award abstract:</strong></em><br />
The calcifying Haptophyte <em>Emiliania huxleyi </em>appears to be acutely sensitive to the rising concentration of ocean pCO2. Documented responses by <em>E. huxleyi </em>to elevated pCO2 include modifications to their calcification rate and cell size, malformation of coccoliths, elevated growth rates, increased organic carbon production, lowering of PIC:POC ratios, and elevated production of the active climate gas DMS. Changes in these parameters are mechanisms known to elicit alterations in grazing behavior by microzooplankton, the oceans dominant grazer functional group. The investigators hypothesize that modifications to the physiology and biochemistry of calcifying and non-calcifying Haptophyte <em>Emiliania huxleyi </em>in response to elevated pCO2 will precipitate alterations in microzooplankton grazing dynamics. To test this hypothesis, they will conduct controlled laboratory experiments where several strains of <em>E. huxleyi</em> are grown at several CO2 concentrations. After careful characterization of the biochemical and physiological responses of the <em>E. huxleyi</em> strains to elevated pCO2, they will provide these strains as food to several ecologically-important microzooplankton and document grazing dynamics.<em> E. huxleyi</em> is an ideal organism for the study of phytoplankton and microzooplankton responses to rising anthropogenic CO2, the effects of which in the marine environment are called ocean acidification; <em>E. huxleyi </em>is biogeochemically important, is well studied, numerous strains are in culture that exhibit variation in the parameters described above, and they are readily fed upon by ecologically important microzooplankton.</p>
<p>The implications of changes in microzooplankton grazing for carbon cycling, specifically CaCO3 export, DMS production, nutrient regeneration in surface waters, and carbon transfer between trophic levels are profound, as this grazing, to a large degree, regulates all these processes. <em>E. huxleyi </em>is a model prey organism because it is one of the most biogeochemically influential global phytoplankton. It forms massive seasonal blooms, contributes significantly to marine inorganic and organic carbon cycles, is a large producer of the climatically active gas DMS, and is a source of organic matter for trophic levels both above and below itself. The planned controlled study will increase our knowledge of the mechanisms that drive patterns of change between trophic levels, thus providing a wider array of tools necessary to understand the complex nature of ocean acidification field studies, where competing variables can confound precise interpretation.</p>
E Hux Response to pCO2
largerWorkCitation
project
eng; USA
biota
oceans
Bellingham, WA
2016-10-18
0
BCO-DMO catalogue of parameters from Microzooplankton ingestion on low to high pCO2 acclimated E. huxleyi: Favella and Oxyrrhis as grazers (E Hux Response to pCO2 project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/661459.rdf
Name: expt
Units: unitless
Description: <p>experiment identification</p>
http://lod.bco-dmo.org/id/dataset-parameter/661460.rdf
Name: grazer_analyzed
Units: unitless
Description: <p>individual grazer identification</p>
http://lod.bco-dmo.org/id/dataset-parameter/661461.rdf
Name: treatment_rep_min
Units: unitless
Description: <p>pCO2 level (ambient; moderate; high); replicate (A; B; C); minutes allowed for ingestion replictate</p>
http://lod.bco-dmo.org/id/dataset-parameter/661462.rdf
Name: cells_ingested
Units: cells
Description: <p>E. huxleyi cells counted inside each grazer</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
194017
https://datadocs.bco-dmo.org/file/1VVMO1kiOOjOYB/expt2_3_ingestion_sort.csv
expt2_3_ingestion_sort.csv
Primary data file for dataset ID 661421
download
https://osprey.bco-dmo.org/dataset/661421/data/download
download
onLine
dataset
<p>The phytoplankton Emiliania huxleyi CCMP 2668 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv). Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients.</p>
<p>Expt. 2: On day 10, after ~ 20 generations, E. huxleyi cells from the treatments were fed to starved Favella taraikaensis cells for 15, 30 and 45 minutes. During each sampling time point, 20 mls of experiment volume was removed, fixed with glutaraldehyde and stained with DAPI. This volume was filtered onto a 20 µm pore size polycarbonate filter with, which was then put on a slide with immersion oil and kept frozen until evaluation.</p>
<p>Expt. 3: On day 10, after ~ 20 generations, E. huxleyi cells from the treatments were fed to starved Oxyrrhis marina cells for 30, 60 and 90 minutes.&nbsp;During each sampling time point, 20 mls of experiment volume was removed, fixed with glutaraldehyde and stained with DAPI. This volume was filtered onto a 10 µm pore size polycarbonate filter with, which was then put on a slide with immersion oil and kept frozen until evaluation.</p>
<p>Slides were evaluated under 1000x oil immersion using an epi-fluorescent microscope under blue-light excitation. The first 100 microzooplankton on each slide were assessed for each replicate/treatment, and individual prey cell were counted in the grazer food vacuole.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- column names reformatted to comply with BCO-DMO standards<br />
- transformed table columns to rows<br />
- nd (no data) was entered into all blank cells<br />
- experiments 2 and 3 data were concatenated into one data set<br />
- sorted data by experiment and treatment</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: PI Supplied Instrument Description:epi-fluorescent microscope under blue-light excitation Instrument Name: Fluorescence Microscope Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB06/
Deployment: Lab_Olson_B
Lab_Olson_B
WWU
laboratory
WWU
laboratory