http://lod.bco-dmo.org/id/dataset/661899
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2016-10-18
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS) data from seasonal collections, Doboy Sound, Sapelo Island, GA, 2012 and 2013
2016-10-26
publication
2016-10-26
revision
BCO-DMO Linked Data URI
2016-10-26
creation
http://lod.bco-dmo.org/id/dataset/661899
Mary Ann Moran
University of Georgia
principalInvestigator
Patricia M. Medeiros
University of Georgia
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Moran, M., Medeiros, P. M. (2016) Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS) data from seasonal collections, Doboy Sound, Sapelo Island, GA, 2012 and 2013. Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2016-10-26 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/661899 [access date]
FT-ICR MS data from samples collected during the SIMCO pilot study Dataset Description: <p>Field collected DOC from estuarine waters near Sapelo Island, GA in 2012 and 2013 was analyzed for chemical composition. Analysis of the dissolved organic matter pool retrieved by solid phase extraction (PPL resin) was analyzed to determine chemical formulas (by Fourier transform ion cyclotron resonance mass spectrometry, FT-ICR MS).</p> Methods and Sampling: <p>DOM was collected in Doboy Sound off the southeastern U.S. in November 2012, May 2013 and September 2013. At least 3 replicates were collected for each sample. Immediately after collection, samples were filtered (using 0.7 μm Whatman GF/F filters pre-combusted at 450°C for 5 h and pre-washed 0.2 μm Pall Supor membrane filters), and aliquots were stored frozen for DOC analysis. Filtrates were acidified to pH 2, and DOM was isolated using solid phase extraction (SPE) cartridges (Agilent Bond Elut PPL) as in Dittmar et al. (2008).</p>
<p>DOC concentrations from water samples and extracts (i.e., dried and resuspended in ultrapure water) were measured with a Shimadzu TOC-VCPH analyzer. SPE efficiency across all samples was 75±7% of the DOC.</p>
<p>Bulk delta-13C ratios of extracted DOC (SPE-DOC) were measured with a Finnigan MAT 251 isotope ratio mass spectrometer after complete drying. Precision and accuracy was better than 1 ppt, and procedural blanks did not yield detectable amounts of carbon isotopes. The molecular composition of the DOM extracts (15 mg C L-1 in 1:1 methanol/water) was analyzed using a 15T Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS; Bruker Daltonics) with electrospray ionization (negative mode) as in Seidel et al. (2014).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1356010 Award URL: http://www.nsf.gov/awardsearch/showAward?AWD_ID=1356010
completed
Mary Ann Moran
University of Georgia
706-542-6481
Department of Marine Sciences
Athens
GA
30602
USA
mmoran@uga.edu
pointOfContact
Patricia M. Medeiros
University of Georgia
706-542-6744
Department of Marine Sciences
Athens
GA
30602-3636
USA
medeiros@uga.edu
pointOfContact
asNeeded
Unknown
mass_to_charge
C
H
O
N
S
P
NOV_2012_A
NOV_2012_B
NOV_2012_C
MAY_2013_A
MAY_2013_B
MAY_2013_C
SEP_2013_A
SEP_2013_B
SEP_2013_C
Finnigan MAT 251 isotope ratio mass spectrometer
Shimadzu TOC-VCPH analyzer
FT-ICR MS (Bruker Daltonics)
theme
None, User defined
mass-to-charge ratio (m/z)
element
percent composition
featureType
BCO-DMO Standard Parameters
Isotope-ratio Mass Spectrometer
Shimadzu TOC-V Analyzer
Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
instrument
BCO-DMO Standard Instruments
Moran_Sapelo_2012-14
service
Deployment Activity
Sapelo Island, GA
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
High Resolution Linkages Between DOC Turnover and Bacterioplankton in a Coastal Ocean
https://www.bco-dmo.org/project/472758
High Resolution Linkages Between DOC Turnover and Bacterioplankton in a Coastal Ocean
<p><em>Description from NSF award abstract:</em><br />
Long-standing questions regarding the fate of dissolved organic carbon (DOC) in coastal oceans require a better understanding of the network that links bacterioplankton metabolism with carbon transformation. These questions address uncertainties about the composition of the bioreactive DOC components transformed in ocean margins, and the role of bacterial taxonomic and genetic composition in determining the fate of DOC.</p>
<p>This project will infuse a new type of data into coastal carbon cycle research based on high-resolution chemical analysis coupled with bacterial gene expression measures. It will extend DOC process studies down to the single-compound level and bacterial activity studies down to the single-gene level, and integrate this information into existing bioinformatic resources for biogeochemical and modeling applications.</p>
<p>The specific goals of this project are:<br />
1) To reconstruct major components of the network linking DOC composition, DOC turnover, and bacterial heterotrophy in the coastal ocean (the composition of the DOC pool, the major bioreactive components, the bacterioplankton taxa mediating transformations, and the bacterial genes and pathways responsible).</p>
<p>2) To test hypothesized network links for selected DOC compounds using a simplified system that queries individual DOC compounds against a complex natural microbial community.</p>
<p>3) To test hypothesized network links for marine bacteria using a simplified system that queries a single generalist heterotrophic bacteria against a complex natural DOC pool.</p>
<p>4) To verify predicted DOC-gene linkages that are most informative about heterotrophic activities of bacterioplankton.</p>
<p>This research addresses fundamental questions on bacterial mediation of organic carbon fate in the ocean and atmosphere. As such, these investigations linking the chemical changes in dissolved organic carbon with patterns of gene expression in coastal bacterioplankton communities will be of interest to scientists across several disciplines.</p>
<p>---------------------------</p>
<p>Note: The project acronym, SIMCO, means "Sapelo Island Microbial Carbon Observatory".</p>
SIMCO
largerWorkCitation
project
eng; USA
oceans
Sapelo Island, GA
2016-10-26
Southeastern U.S. coastal ocean, 31.4° N Lat, 81.3° W Lon
0
BCO-DMO catalogue of parameters from Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS) data from seasonal collections, Doboy Sound, Sapelo Island, GA, 2012 and 2013
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/662330.rdf
Name: mass_to_charge
Units: Daltons (Da)
Description: mass-to-charge ratio (m/z). This is equivalent to the mass of a molecule since charge is 1
http://lod.bco-dmo.org/id/dataset-parameter/662331.rdf
Name: C
Units: atoms
Description: number of carbon atoms in molecule
http://lod.bco-dmo.org/id/dataset-parameter/662332.rdf
Name: H
Units: atoms
Description: number of hydrogen atoms in molecule
http://lod.bco-dmo.org/id/dataset-parameter/662333.rdf
Name: O
Units: atoms
Description: number of oxygen atoms in molecule
http://lod.bco-dmo.org/id/dataset-parameter/662334.rdf
Name: N
Units: atoms
Description: number of nitrogen atoms in molecule
http://lod.bco-dmo.org/id/dataset-parameter/662335.rdf
Name: S
Units: atoms
Description: number of sulfur atoms in molecule
http://lod.bco-dmo.org/id/dataset-parameter/662336.rdf
Name: P
Units: atoms
Description: number of phosphorous atoms in molecule
http://lod.bco-dmo.org/id/dataset-parameter/662337.rdf
Name: NOV_2012_A
Units: unitless
Description: relative abundance of molecule in sample Nov_2012_A
http://lod.bco-dmo.org/id/dataset-parameter/662338.rdf
Name: NOV_2012_B
Units: unitless
Description: relative abundance of molecule in sample Nov_2012_B
http://lod.bco-dmo.org/id/dataset-parameter/662339.rdf
Name: NOV_2012_C
Units: unitless
Description: relative abundance of molecule in sample Nov_2012_C
http://lod.bco-dmo.org/id/dataset-parameter/662340.rdf
Name: MAY_2013_A
Units: unitless
Description: relative abundance of molecule in sample May_2013_A
http://lod.bco-dmo.org/id/dataset-parameter/662341.rdf
Name: MAY_2013_B
Units: unitless
Description: relative abundance of molecule in sample May_2013_B
http://lod.bco-dmo.org/id/dataset-parameter/662342.rdf
Name: MAY_2013_C
Units: unitless
Description: relative abundance of molecule in sample May_2013_C
http://lod.bco-dmo.org/id/dataset-parameter/662343.rdf
Name: SEP_2013_A
Units: unitless
Description: relative abundance of molecule in sample Sep_2013_A
http://lod.bco-dmo.org/id/dataset-parameter/662344.rdf
Name: SEP_2013_B
Units: unitless
Description: relative abundance of molecule in sample Sep_2013_B
http://lod.bco-dmo.org/id/dataset-parameter/662345.rdf
Name: SEP_2013_C
Units: unitless
Description: relative abundance of molecule in sample Sep_2013_C
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
379490
https://datadocs.bco-dmo.org/file/yppzBlNTJOgpq6/FTICRMS.csv
FTICRMS.csv
Primary data file for dataset ID 661899
download
https://www.bco-dmo.org/dataset/661899/data/download
download
onLine
dataset
<p>DOM was collected in Doboy Sound off the southeastern U.S. in November 2012, May 2013 and September 2013. At least 3 replicates were collected for each sample. Immediately after collection, samples were filtered (using 0.7 μm Whatman GF/F filters pre-combusted at 450°C for 5 h and pre-washed 0.2 μm Pall Supor membrane filters), and aliquots were stored frozen for DOC analysis. Filtrates were acidified to pH 2, and DOM was isolated using solid phase extraction (SPE) cartridges (Agilent Bond Elut PPL) as in Dittmar et al. (2008).</p>
<p>DOC concentrations from water samples and extracts (i.e., dried and resuspended in ultrapure water) were measured with a Shimadzu TOC-VCPH analyzer. SPE efficiency across all samples was 75±7% of the DOC.</p>
<p>Bulk delta-13C ratios of extracted DOC (SPE-DOC) were measured with a Finnigan MAT 251 isotope ratio mass spectrometer after complete drying. Precision and accuracy was better than 1 ppt, and procedural blanks did not yield detectable amounts of carbon isotopes. The molecular composition of the DOM extracts (15 mg C L-1 in 1:1 methanol/water) was analyzed using a 15T Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS; Bruker Daltonics) with electrospray ionization (negative mode) as in Seidel et al. (2014).</p>
Specified by the Principal Investigator(s)
<p><strong>References:</strong></p>
<p>Dittmar, T., Boris Koch, Norbert Hertkorn, and Gerhard Kattner. 2008. A simple and efficient method for the solid-phase extraction of dissolved organic matter (SPE-DOM) from seawaterLimnol. Oceanogr.: Methods 6, 2008, 230-235</p>
<p>Medeiros, P. M., M. Seidel, T. Dittmar, W. B. Whitman, and M. A. Moran (2015), Drought-induced variability in dissolved organic matter composition in a marshdominated estuary, Geophys. Res. Lett., 42, 6446–6453, doi:10.1002/ 2015GL064653.</p>
<p>Seidel, M., Melanie Beck, Thomas Riedel, Hannelore Waska, I G.N.A. Suryaputra, d, Bernhard Schnetger, Jutta Niggemann, Meinhard Simonc, Thorsten Dittmar. 2014. Biogeochemistry of dissolved organic matter in an anoxic intertidal creek bank. Geochimica et Cosmochimica Acta 140:418-434. http://dx.doi.org/10.1016/j.gca.2014.05.038</p>
<p><strong>BCO-DMO Processing:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- column names reformatted to comply with BCO-DMO standards<br />
- replaced spaces with underscores</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Finnigan MAT 251 isotope ratio mass spectrometer
Finnigan MAT 251 isotope ratio mass spectrometer
PI Supplied Instrument Name: Finnigan MAT 251 isotope ratio mass spectrometer PI Supplied Instrument Description:Bulk delta-13C ratios of extracted DOC (SPE-DOC) were measured Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Shimadzu TOC-VCPH analyzer
Shimadzu TOC-VCPH analyzer
PI Supplied Instrument Name: Shimadzu TOC-VCPH analyzer PI Supplied Instrument Description:DOC concentrations measured Instrument Name: Shimadzu TOC-V Analyzer Instrument Short Name:Shimadzu TOC-V Instrument Description: A Shimadzu TOC-V Analyzer measures DOC by high temperature combustion method. Community Standard Description: http://onto.nerc.ac.uk/CAST/124
FT-ICR MS (Bruker Daltonics)
FT-ICR MS (Bruker Daltonics)
PI Supplied Instrument Name: FT-ICR MS (Bruker Daltonics) PI Supplied Instrument Description:The molecular composition of the DOM extracts were analyzed Instrument Name: Fourier Transform Ion Cyclotron Resonance Mass Spectrometer Instrument Short Name:FTICR MS Instrument Description: In Fourier Transform Ion Cyclotron Resonance Mass Spectrometry, the mass-to-charge ratio (m/z) of an ion is experimentally determined by measuring the frequency at which the ion processes in a magnetic field. These frequencies, which are typically in the 100 KHz to MHz regime, can be measured with modern electronics making it possible to determine the mass of an ion to within +/- 0.000005 amu or 5 ppm.
Deployment: Moran_Sapelo_2012-14
Moran_Sapelo_2012-14
Univ_Georgia
laboratory
Moran_Sapelo_2012-14
Mary Ann Moran
University of Georgia
Univ_Georgia
laboratory