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            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset/662036.rdf" xlink:actuate="onRequest">Physiology of phytoplankton collected in coastal waters at Palmer LTER near Palmer Station, Antarctica, 2012 and 2013 (Phytoplankton Seasonal Dynamics project)</gmx:Anchor>
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&amp;lt;p&amp;gt;Detailed description of methods are given in the published peer-reviewed papers listed below:&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Goldman, J., Kranz, S., Young, J.N., Tortell, P.D., Bender, M., Morel, F.M.M. (2015) Gross and net production during the spring bloom along the Western Antarctic Peninsula New Phytologist 205 (1): 182-191. DOI: 10.1111/nph.13125.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Kranz, S., Young, J.N., Goldman, J., Tortell, P.D., Bender, M., Morel, F.M.M. (2015) Low temperature reduces the energetic requirement for the CO2 concentrating mechanism in diatoms. New Phytologist 205 (1): 192-201. DOI:&amp;amp;nbsp;10.1111/nph.12976&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Tortell, P.D., Asher, E.C., Dacey, J.W.H. Kranz, S., Young, J.N., Goldman, J. Ducklow, H., Grzymski, J. Stanley, R., Morel, F.M.M. (2014) Metabolic balance of coastal Antarctic waters revealed by autonomous high frequency pCO2 and dO2 /Ar measurements Geophysical Research Letters DOI: 10.1002/2014GL061266&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Young, J.N., Goldman, J., Kranz, S., Tortell, P.D., Morel, F.M.M. (2015) Slow carboxylation of Rubisco constrains the rate of carbon fixation during Antarctic phytoplankton blooms. New Phytologist 205 (1): 172-181. DOI: 10.1111/nph.13021&amp;lt;/p&amp;gt;</gco:CharacterString>
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                            <gco:CharacterString>&lt;p&gt;&lt;em&gt;Description from NSF award abstract:&lt;/em&gt;&lt;br /&gt;
The Southern Ocean plays a key role in marine biogeochemistry and global climate. Along the Western Antarctic Peninsula (WAP), winter air temperatures have increased by more than 5 ºC over the past five decades, and sea ice duration and extent have decreased substantially, leading to dramatic ecological perturbations. The sensitivity of primary production and climate-active gas cycling to on-going changes in the physical and chemical environment of the WAP is the key to understanding potential biogeochemical climate feedbacks in this region. The objective of this project is to characterize and understand the seasonal dynamics of primary production and climate-active gases carbon dioxide (CO2) and dimethylsulfide (DMS) in the WAP. The project will examine (1) the relationships among seasonal changes in pCO2, temperature and light with respect to gross primary production, net primary production, and net community production; (2) the extent to which changes in surface water pCO2 and temperature drive ecological shifts in the dominant phytoplankton species assemblage composition, and alter key processes in the DMS cycle. The research will employ membrane inlet mass spectrometry to monitor dissolved gases in ambient seawater at the Palmer Station LTER site and conduct physiological/biochemical assays with tracer compounds. The research will provide unprecedented information on the temporal evolution of primary production and dissolved gas concentrations in the WAP in relation to surface hydrography and sea ice cover. Field studies will be supported by laboratory experiments with model species subjected to detailed studies of carbon metabolism. This project will contribute significantly to the understanding of seasonal biogeochemical dynamics in a region that is particularly sensitive to ongoing climate perturbations and important for the global air-sea exchange of CO2.&lt;/p&gt;
&lt;p&gt;&lt;em&gt;Related publications:&lt;/em&gt;&lt;br /&gt;
Young, J.N., Goldman, J., Kranz, S., Tortell, P.D., Morel, F.M.M. (2015) Slow carboxylation of Rubisco constrains the rate of carbon fixation during Antarctic phytoplankton blooms. &lt;em&gt;New Phytologist&lt;/em&gt; 205 (1): 172-181. DOI: &lt;a href=&quot;http://dx.doi.org/10.1111/nph.13021&quot; target=&quot;_blank&quot;&gt;10.1111/nph.13021&lt;/a&gt;&lt;/p&gt;
&lt;p&gt;Goldman, J., Kranz, S., Young, J.N.&lt;strong&gt;,&lt;/strong&gt; Tortell, P.D., Bender, M., Morel, F.M.M. (2015) Gross and net production during the spring bloom along the Western Antarctic Peninsula &lt;em&gt;New Phytologist &lt;/em&gt;205 (1): 182-191. DOI: &lt;a href=&quot;http://dx.doi.org/10.1111/nph.13125&quot; target=&quot;_blank&quot;&gt;10.1111/nph.13125&lt;/a&gt;&lt;/p&gt;
&lt;p&gt;Kranz, S., Young, J.N., Goldman, J., Tortell, P.D., Bender, M., Morel, F.M.M. (2015) Low temperature reduces the energetic requirement for the CO&lt;sub&gt;2&lt;/sub&gt; concentrating mechanism in diatoms. &lt;em&gt;New Phytologist&lt;/em&gt; 205 (1): 192-201. DOI: &lt;a href=&quot;http://dx.doi.org/10.1111/nph.12976&quot; target=&quot;_blank&quot;&gt;10.1111/nph.12976&lt;/a&gt;&lt;/p&gt;
&lt;p&gt;Tortell, P.D., Asher, E.C., Dacey, J.W.H. Kranz, S.,&lt;strong&gt; &lt;/strong&gt;Young, J.N., Goldman, J. Ducklow, H., Grzymski, J. Stanley, R., Morel, F.M.M. (2014) Metabolic balance of coastal Antarctic waters revealed by autonomous high frequency &lt;em&gt;p&lt;/em&gt;CO&lt;sub&gt;2&lt;/sub&gt; and dO&lt;sub&gt;2&lt;/sub&gt; /Ar measurements Geophysical Research Letters DOI: &lt;a href=&quot;http://dx.doi.org/10.1002/2014GL061266&quot; target=&quot;_blank&quot;&gt;10.1002/2014GL061266&lt;/a&gt;&lt;/p&gt;</gco:CharacterString>
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	Name: date_plot
	Units: year-month-day
	Description: &lt;p&gt;sampling date (UTC), formatted to allow sequential plotting of time variable&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662225.rdf
	Name: temp
	Units: degrees celsius
	Description: &lt;p&gt;As measured by CTD at 10 m depth&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662226.rdf
	Name: sal
	Units: parts per thousand
	Description: &lt;p&gt;Salinity&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662227.rdf
	Name: pCO2
	Units: ppm
	Description: &lt;p&gt;CO2 partial pressure. Calculated from TA; pH;  temperature  salinity;  phosphate and silicate using CO2Sys&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662228.rdf
	Name: CO2_aq
	Units: micromole/kilogram
	Description: &lt;p&gt;CO2 aqueous. Calculated from TA; pH; temperature; salinity; phosphate and silicate using CO2Sys&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662229.rdf
	Name: TA_meas
	Units: micromole/kilogram
	Description: &lt;p&gt;Total alkalinity. Measured in 0.2micron filtered and poisoned seawater by potentiometric titration with average precision of +/- 7 micromole/kg&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662230.rdf
	Name: TA_sd
	Units: micromole/kilogram
	Description: &lt;p&gt;Total alkalinity standard deviation&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662231.rdf
	Name: DIC_cal
	Units: micromole/kilogram
	Description: &lt;p&gt;Dissolved Inorganic Carbon. Calculated from TA; pH; temperature; salinity; phosphate and silicate using CO2Sys&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662232.rdf
	Name: pH
	Units: total scale
	Description: &lt;p&gt;calibrated potentiometrey (until mid-Dec) and spectrophotometrically (rest) and adjusted for temperature&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662233.rdf
	Name: NO3_NO2_diss_avg
	Units: micromolar
	Description: &lt;p&gt;Dissolved nitrate and nitrite&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662234.rdf
	Name: NO3_NO2_diss_stdev
	Units: micromolar
	Description: &lt;p&gt;Dissolved nitrate and nitrite standard deviation&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662235.rdf
	Name: SiO4_diss_avg
	Units: micromolar
	Description: &lt;p&gt;Dissolved silicate&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662236.rdf
	Name: SiO4_diss_stdev
	Units: micromolar
	Description: &lt;p&gt;Dissolved silicate standard deviation&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662237.rdf
	Name: PO4_diss_avg
	Units: micromolar
	Description: &lt;p&gt;Dissolved phosphate&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662238.rdf
	Name: PO4_diss_stdev
	Units: micromolar
	Description: &lt;p&gt;Dissolved phosphate standard deviation&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662239.rdf
	Name: Chl_a_avg
	Units: micrograms/liter
	Description: &lt;p&gt;Chlorophyll a concentrations; as measured with turner fluorometer&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662240.rdf
	Name: Chl_a_stdev
	Units: micrograms/liter
	Description: &lt;p&gt;Chlorophyll a standard deviation&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662241.rdf
	Name: NPP_14C_avg
	Units: C (micromoles)/liter/day
	Description: &lt;p&gt;Net Primary Production as measured by 14C bottle incubations (24 h)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662242.rdf
	Name: NPP_14C_stdev
	Units: C (micromoles)/liter/day
	Description: &lt;p&gt;NPP 14C standard deviation&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662243.rdf
	Name: GPP_14C_avg
	Units: C (micromoles)/liter/day
	Description: &lt;p&gt;Gross Primary Production as measured by 14C bottle incubations (2 h)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662244.rdf
	Name: GPP_14C_stdev
	Units: C (micromoles)/liter/day
	Description: &lt;p&gt;GPP 14C standard deviation&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662245.rdf
	Name: GPP_18O_avg
	Units: O2 micromoles/liter/day
	Description: &lt;p&gt;Gross Primary Production as measured by production of 18O2 from H218O water (~6h)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662246.rdf
	Name: GPP_18O_stdev
	Units: O2 micromoles/liter/day
	Description: &lt;p&gt;GPP 18O standard deviation&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662247.rdf
	Name: NPP_O2_Ar_avg
	Units: O2 micromoles/liter/day
	Description: &lt;p&gt;Net Primary Production as measured by change in O2 with respect to Argon in bottle incubations  (~6h)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662248.rdf
	Name: NPP_O2_Ar_stdev
	Units: O2 micromoles/liter/day
	Description: &lt;p&gt;NPP O2/Ar standard deviation&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662249.rdf
	Name: POC
	Units: micromoles/liter
	Description: &lt;p&gt;Particulate Organic Carbon as measured on Elemental Combustion Analyzer&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662250.rdf
	Name: PON
	Units: micromoles/liter
	Description: &lt;p&gt;Particulate Nitrogen as measured on Elemental Combustion Analyzer&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662251.rdf
	Name: C_N
	Units: mol:mol
	Description: &lt;p&gt;Carbon to nitrogen ratio of particulate matter; calcuated from POC and PON&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662252.rdf
	Name: POC_d13C
	Units: per mil
	Description: &lt;p&gt;Isotopic ratio of 13C to 12C in POC normalised to PDB standard&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662253.rdf
	Name: Protein
	Units: microgram/liter
	Description: &lt;p&gt;Total protein from particulate matter as measured by BCA Assay&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662254.rdf
	Name: Rubisco
	Units: % total protein (mg:mg)
	Description: &lt;p&gt;Percent of the carboxylating enzyme Ribulose 1;5 bisphosphate carboxylase oxygenase (Rubisco) as measured by quantitative immunoblotting&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662255.rdf
	Name: Fuco
	Units: micrograms/liter
	Description: &lt;p&gt;pigment concentration of fucoxanthin; as measured by HPLC&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662256.rdf
	Name: HexFuc19
	Units: micrograms/liter
	Description: &lt;p&gt;pigment concentration of 19&amp;#039;Hexanoyloxyfucoxanthin; as measured by HPLC&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662257.rdf
	Name: Allox
	Units: micrograms/liter
	Description: &lt;p&gt;pigment concentration of alloxanthin; as measured by HPLC&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662258.rdf
	Name: diatom
	Units: cells/milliliter
	Description: &lt;p&gt;Calculated from pigments using equation 0.9*(Fuco-0.05*19&amp;#039;HexFuc)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662259.rdf
	Name: Phaeo
	Units: cells/milliliter
	Description: &lt;p&gt;Calculated from pigments using equation 1.25*19&amp;#039;HexFuc&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662260.rdf
	Name: Cryptophyte
	Units: cells/milliliter
	Description: &lt;p&gt;Calculated from pigments using equation 0.7*Allox&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662261.rdf
	Name: eCA
	Units: milligrams Chl-a/liter (mg/L)
	Description: &lt;p&gt;Activity of external carbonic anhydrase (eCA) using the depletion of 18O of aqueous 13C18O2 caused by hydration and dehydration steps of CO2 and bicarbonate. Shown as the ratio of catalyzed CO2/bicarbonate conversion rate compared to the uncatalyzed rate and normalised to chlorophyll. Units are Fold Activity compared to uncatalyzed rate; normalised to chlorophyll concentrations.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662262.rdf
	Name: fHCO3_avg
	Units: dimensionless
	Description: &lt;p&gt;Proportion of bicarbonate of the total inorganic carbon taken up by the cells to support photosynthesis. Measured using the disquilibrium technique&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662263.rdf
	Name: fHCO3_stdev
	Units: dimensionless
	Description: &lt;p&gt;fHCO3 standard deviation&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662264.rdf
	Name: K1_2_CO2_avg
	Units: micromolar
	Description: &lt;p&gt;Michaelis-Menten half-saturation constant for the CO2 concentration that results in half maximum rate of carbon fixation rate of the natural phytoplankton community concentrated onto a 2micron filter&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662265.rdf
	Name: K1_2_CO2_stdev
	Units: micromolar
	Description: &lt;p&gt;K1/2(CO2) standard deviation&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662266.rdf
	Name: lat
	Units: decimal degrees
	Description: &lt;p&gt;latitude; north is positive&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662267.rdf
	Name: lon
	Units: decimal degrees
	Description: &lt;p&gt;longitude; east is positive&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662283.rdf
	Name: year
	Units: year
	Description: &lt;p&gt;sampling year&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/662288.rdf
	Name: julian_day
	Units: days
	Description: &lt;p&gt;day of year&lt;/p&gt; 
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&amp;lt;p&amp;gt;Goldman, J., Kranz, S., Young, J.N., Tortell, P.D., Bender, M., Morel, F.M.M. (2015) Gross and net production during the spring bloom along the Western Antarctic Peninsula New Phytologist 205 (1): 182-191. DOI: 10.1111/nph.13125.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Kranz, S., Young, J.N., Goldman, J., Tortell, P.D., Bender, M., Morel, F.M.M. (2015) Low temperature reduces the energetic requirement for the CO2 concentrating mechanism in diatoms. New Phytologist 205 (1): 192-201. DOI:&amp;amp;nbsp;10.1111/nph.12976&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Tortell, P.D., Asher, E.C., Dacey, J.W.H. Kranz, S., Young, J.N., Goldman, J. Ducklow, H., Grzymski, J. Stanley, R., Morel, F.M.M. (2014) Metabolic balance of coastal Antarctic waters revealed by autonomous high frequency pCO2 and dO2 /Ar measurements Geophysical Research Letters DOI: 10.1002/2014GL061266&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Young, J.N., Goldman, J., Kranz, S., Tortell, P.D., Morel, F.M.M. (2015) Slow carboxylation of Rubisco constrains the rate of carbon fixation during Antarctic phytoplankton blooms. New Phytologist 205 (1): 172-181. DOI: 10.1111/nph.13021&amp;lt;/p&amp;gt;</gco:CharacterString>
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