http://lod.bco-dmo.org/id/dataset/663183
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2016-10-26
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Particulate data collected on R/V Melville (MV1405, IrnBru) along the California coast in July 2014
2016-10-26
publication
2016-10-26
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2023-09-11
publication
https://doi.org/10.26008/1912/bco-dmo.663183.1
Benjamin Twining
Bigelow Laboratory for Ocean Sciences
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Twining, B. (2023) Particulate data collected on R/V Melville (MV1405, IrnBru) along the California coast in July 2014. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2016-10-26 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.663183.1 [access date]
IronBru particulate data organized by station and transect. Dataset Description: <p>Trace element concentrations in particles collected with GO-Flo bottles and analyzed with inductively-coupled plasma mass spectrometry (ICP-MS). Concentrations of labile, refractory, and total metal fractions are reported.</p> Methods and Sampling: <p><strong>Total, refractory, and labile particulate element concentrations via ICPMS:</strong><br />
Labile and total suspended particulate trace elements concentrations are reported for: Al, Ba, Cd, Co, Cu, Fe, La, Mn, Ni, P, Pb, Sc, Th, Ti, V, Y, Zn. Concentrations of the labile fraction of these particulate elements are indicated as element names followed by the suffix ‘-Labile’, refractory portions are indicated with the suffix ‘-Refractory’, and concentrations of total particulate elements (the sum of labile and refractory) are followed by ‘-Total’. Concentrations are reported in units of picomoles per liter (pmol/L).</p>
<p><strong>Sampling Methodology:</strong><br />
Trace metal-clean seawater samples were collected using a sampling system consisting of Teflon-coated GO-Flo bottles and following methods described in Bruland et al (1979). Additional samples were collected from surface waters (~2 meters) using a towed ‘fish’ deployed by Ken Bruland's lab. Water collected with GO-Flo bottles and the ‘fish’ was transferred into acid-washed 4-liter (L) LDPE carboys for off-line filtration.</p>
<p>All filtration was conducted in a HEPA-filtered 'bubble' (temporary clean room). A filter holder containing a 25-millimeter (mm) diameter Pall Supor 0.4-micrometer (um) polyethersulfone membrane was attached to the opening at the carboy top. Carboys were pressurized with 0.2‐um filtered air and inverted during filtration to ensure that all particles were captured on the membrane. Filtrate was collected to determine the volume of seawater filtered: an average of 2.1L was filtered through each membrane. After filtration, membranes were folded into quarters, placed in 1.7-milliliter (mL) polypropylene vials, and stored at -20 degrees Celsius until analysis.</p>
<p><strong>Analytical Methodology:</strong><br />
All digestion steps were performed in a Class-100 clean room using standard clean techniques. Filters were sequentially digested, first following the protocol of Berger et al. (2008) to obtain labile particulate concentrations and then digested using a 4M HCl, 4M HNO3, and 4M HF mixture as described in Ohnemus et al. (2014) to obtain refractory particulate element concentrations.</p>
<p>For the labile particulate leach, a 1-milliliter solution of 25% Optima-grade acetic acid and 0.02 M hydroxylamine was added to the filter stored in a 1.7 mL polypropylene vial. Following the recommendation of Berger et al. (2008), the solution was heated to 95 degrees C in a water bath for 10 minutes and then allowed to cool to room temperature. The filter was in contact with the acetic acid leach for a total of two hours, after which the filter was removed from the polypropylene vial and placed in an acid-cleaned 22-mL PFA vial. The acetic acid/hydroxylamine leachate was centrifuged at 14,000 rpm for 10 minutes to sediment all particles. Without disturbing particles on the bottom of the tube, approximately 0.8 mL of leachate was transferred into an acid-cleaned 7 mL PFA digestion vial. Optima-grade HNO3 was added (100 uL) to the digestion vial, which was subsequently heated uncapped at 110 degrees C to near dryness. Vial contents were redissolved with 2% HNO3 (Optima grade).</p>
<p>Refractory particulate metals were determined by subsequent digestion of the filter. Two milliliters of a solution containing 4M HCl, 4M HNO3, and 4M HF (all Optima grade) was added to the filter which was placed in a cleaned 22-mL PFA vial. The vial was tightly capped and heated to 110°C for 4 hours. This procedure has been determined to be adequate for digestion of all particulate material, while allowing the Supor filter to remain intact (Ohnemus et al. 2014). Following heating, the acid solution in the bomb was poured into a second PFA vial, leaving the filter piece behind. To ensure complete transfer of acid, the bombs were thoroughly rinsed with 3 × 0.5 mL aliquots of ultrapure water which were poured into the secondary vial. The secondary vial was then heated to dryness and the contents redissolved with 1 mL of a 50% Optima-grade HNO3 + 15% Optima-grade H2O2 (v/v of concentrated reagents) solution. This solution was again dried down and the contents redissolved with 2% HNO3.</p>
<p>All digests were analyzed using a Finnigan-MAT Element2 HR-ICP-MS at the University of Maine following the protocols outlined in Twining et al. (2011). The instrument is equipped with a cyclonic nebulizer, an autosampler contained under a HEPA filter, and nickel cones. Ba, Cd, La, Th, and Y were analyzed in low-resolution mode, while the remaining isotopes were analyzed in medium-resolution mode.</p>
<p>Quantification was performed by external calibration, and In-115 was used as an internal standard to correct for variations in instrumental sensitivity during analyses. Cs-133, spiked during the initial sample leaches, was used as a process recovery monitor, but no samples were discarded or corrected using the Cs recoveries, as typical Cs recoveries were 90-110%. Certified reference materials were digested alongside refractory sample digests. Average recoveries for each element are given in the attached supplemental file "CRMs.png".</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1334632 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1334632
completed
Benjamin Twining
Bigelow Laboratory for Ocean Sciences
207-315-2567
60 Bigelow Drive PO Box 380
East Boothbay
ME
04544-0380
USA
btwining@bigelow.org
pointOfContact
asNeeded
Dataset Version: 1
Unknown
description
station
depth
lat
lon
date
time_gmt
Cd_Labile
La_Labile
Y_Labile
Pb_Labile
Ba_Labile
Th_Labile
Al_Labile
Mn_Labile
Fe_Labile
Co_Labile
Cu_Labile
Ni_Labile
P_Labile
Ti_Labile
V_Labile
Zn_Labile
Sc_Labile
Cd_Refractory
La_Refractory
Y_Refractory
Pb_Refractory
Ba_Refractory
Th_Refractory
Al_Refractory
Mn_Refractory
Fe_Refractory
Co_Refractory
Cu_Refractory
Ni_Refractory
P_Refractory
Ti_Refractory
V_Refractory
Zn_Refractory
Sc_Refractory
Cd_Total
La_Total
Y_Total
Pb_Total
Ba_Total
Th_Total
Al_Total
Mn_Total
Fe_Total
Co_Total
Cu_Total
Ni_Total
P_Total
Ti_Total
V_Total
Zn_Total
Sc_Total
Cd_LabileFlag
La_LabileFlag
Y_LabileFlag
Pb_LabileFlag
Ba_LabileFlag
Th_LabileFlag
Al_LabileFlag
Mn_LabileFlag
Fe_LabileFlag
Co_LabileFlag
Cu_LabileFlag
Ni_LabileFlag
P_LabileFlag
Ti_LabileFlag
V_LabileFlag
Zn_LabileFlag
Sc_LabileFlag
Cd_RefrFlag
La_RefrFlag
Y_RefrFlag
Pb_RefrFlag
Ba_RefrFlag
Th_RefrFlag
Al_RefrFlag
Mn_RefrFlag
Fe_RefrFlag
Co_RefrFlag
Cu_RefrFlag
Ni_RefrFlag
P_RefrFlag
Ti_RefrFlag
V_RefrFlag
Zn_RefrFlag
Sc_RefrFlag
Cd_TotalFlag
La_TotalFlag
Y_TotalFlag
Pb_TotalFlag
Ba_TotalFlag
Th_TotalFlag
Al_TotalFlag
Mn_TotalFlag
Fe_TotalFlag
Co_TotalFlag
Cu_TotalFlag
Ni_TotalFlag
P_TotalFlag
Ti_TotalFlag
V_TotalFlag
Zn_TotalFlag
Sc_TotalFlag
ISO_DateTime_UTC
Towed fish
Teflon-coated GO-Flo Bottle
theme
None, User defined
sample identification
station
depth
latitude
longitude
date
time_gmt
trace element concentration
quality flag
ISO_DateTime_UTC
featureType
BCO-DMO Standard Parameters
GeoFish Towed near-Surface Sampler
GO-FLO Teflon Trace Metal Bottle
instrument
BCO-DMO Standard Instruments
MV1405
service
Deployment Activity
California Coastline
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Investigating the Ecological Importance of Iron Storage in Diatoms
https://osprey.bco-dmo.org/project/527114
Collaborative Research: Investigating the Ecological Importance of Iron Storage in Diatoms
<p><em>NSF Award Abstract:</em><br />
Diatoms are responsible for a significant fraction of primary production in the ocean. They are associated with enhanced carbon export and usually dominate the response of phytoplankton to additions of the micronutrient iron in high-nutrient, low-chlorophyll (HNLC) regions. Diatoms, particularly those isolated from the open ocean, appear to have a significant capacity to store iron for later use, and in some groups of diatoms this ability is enabled by the iron storage protein ferritin. Such luxury uptake of iron has long been observed in laboratory cultures and hypothesized to provide diatoms with an ecological benefit in the low-iron waters that cover 40% of the global ocean. However iron storage has been difficult to observe in natural systems due to the methodological challenges of working with mixed plankton assemblages, and a physiological understanding of the impacts of iron on ocean diatoms is lacking. This project combines state-of-the-art high-throughput transcriptomic sequencing and single-cell element analysis with novel laboratory and field incubation experiments to quantify iron storage abilities of cultured and natural diatoms that either contain or lack ferritin and determine the ecological impacts of this process. The overall objective of this project is to examine the ecological importance of iron storage as a selective mechanism controlling the distributions of diatoms along iron gradients in marine ecosystems. The proposed research includes three specific objectives:</p>
<p>A. Determine if there is a consistent physiological difference in the ability of pennate versus centric diatoms to store iron.</p>
<p>B. Examine whether iron storage capacities across diverse diatom taxa consistently provide a mechanistic explanation for continued growth in the absence of iron.</p>
<p>C. Determine whether enhanced iron storage provides diatoms with a competitive within natural phytoplankton assemblages in both coastal and oceanic regions.</p>
<p>Transcriptomic sequencing on a variety of ecologically important pennate and centric diatoms will be used to survey for the presence of ferritin-like genes in order to establish biogeographical and/or phylogenetic patterns of occurrence of diatom ferritin. Laboratory culture experiments will be used to quantify the iron storage abilities of these diatoms, as well as the number of cell divisions that can be supported by the stored iron, providing valuable physiological data to inform the understanding of plankton ecology in iron-limited coastal and HNLC systems. The laboratory experiments will be complemented by measurements of ferritin expression and iron storage in coastal and ocean diatoms sampled across gradients of iron availability on two cruises-of-opportunity to the northeast Pacific Ocean.</p>
<p>The NCBI bioproject page can be found <a href="https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA320398" target="_blank">here</a>.</p>
Diatom Iron Storage
largerWorkCitation
project
eng; USA
oceans
California Coastline
-126.75
-120.03
34.23
42.866
2014-07-06
2014-07-25
North Pacific, California coast and subarctic gyre
0
BCO-DMO catalogue of parameters from Particulate data collected on R/V Melville (MV1405, IrnBru) along the California coast in July 2014
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/663271.rdf
Name: description
Units: unitless
Description: <p>PI issued sample ID</p>
http://lod.bco-dmo.org/id/dataset-parameter/663272.rdf
Name: station
Units: unitless
Description: <p>Station where sampling occurred</p>
http://lod.bco-dmo.org/id/dataset-parameter/663273.rdf
Name: depth
Units: meters
Description: <p>Depth where sampling occurred</p>
http://lod.bco-dmo.org/id/dataset-parameter/663274.rdf
Name: lat
Units: decimal degrees
Description: <p>Latitude; N is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/663275.rdf
Name: lon
Units: decimal degrees
Description: <p>Longitude; W is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/663276.rdf
Name: date
Units: unitless
Description: <p>Date of sampling; mm/dd/yy</p>
http://lod.bco-dmo.org/id/dataset-parameter/663277.rdf
Name: time_gmt
Units: unitless
Description: <p>Time of sampling; HH:MM</p>
http://lod.bco-dmo.org/id/dataset-parameter/663278.rdf
Name: Cd_Labile
Units: picomole per liter (pmol/L)
Description: <p>Labile concentration of Cadmium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663279.rdf
Name: La_Labile
Units: picomole per liter (pmol/L)
Description: <p>Labile concentration of Lanthanum</p>
http://lod.bco-dmo.org/id/dataset-parameter/663280.rdf
Name: Y_Labile
Units: picomole per liter (pmol/L)
Description: <p>Labile concentration of Yttrium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663281.rdf
Name: Pb_Labile
Units: picomole per liter (pmol/L)
Description: <p>Labile concentration of Lead</p>
http://lod.bco-dmo.org/id/dataset-parameter/663282.rdf
Name: Ba_Labile
Units: picomole per liter (pmol/L)
Description: <p>Labile concentration of Barium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663283.rdf
Name: Th_Labile
Units: picomole per liter (pmol/L)
Description: <p>Labile concentration of Thorium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663284.rdf
Name: Al_Labile
Units: picomole per liter (pmol/L)
Description: <p>Labile concentration of Aluminum</p>
http://lod.bco-dmo.org/id/dataset-parameter/663285.rdf
Name: Mn_Labile
Units: picomole per liter (pmol/L)
Description: <p>Labile concentration of Maganese</p>
http://lod.bco-dmo.org/id/dataset-parameter/663286.rdf
Name: Fe_Labile
Units: picomole per liter (pmol/L)
Description: <p>Labile concentration of Iron</p>
http://lod.bco-dmo.org/id/dataset-parameter/663287.rdf
Name: Co_Labile
Units: picomole per liter (pmol/L)
Description: <p>Labile concentration of Cobalt</p>
http://lod.bco-dmo.org/id/dataset-parameter/663288.rdf
Name: Cu_Labile
Units: picomole per liter (pmol/L)
Description: <p>Labile concentration of Copper</p>
http://lod.bco-dmo.org/id/dataset-parameter/663289.rdf
Name: Ni_Labile
Units: picomole per liter (pmol/L)
Description: <p>Labile concentration of Nickel</p>
http://lod.bco-dmo.org/id/dataset-parameter/663290.rdf
Name: P_Labile
Units: picomole per liter (pmol/L)
Description: <p>Labile concentration of Phosphorus</p>
http://lod.bco-dmo.org/id/dataset-parameter/663291.rdf
Name: Ti_Labile
Units: picomole per liter (pmol/L)
Description: <p>Labile concentration of Titanium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663292.rdf
Name: V_Labile
Units: picomole per liter (pmol/L)
Description: <p>Labile concentration of Vanadium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663293.rdf
Name: Zn_Labile
Units: picomole per liter (pmol/L)
Description: <p>Labile concentration of Zinc</p>
http://lod.bco-dmo.org/id/dataset-parameter/663294.rdf
Name: Sc_Labile
Units: picomole per liter (pmol/L)
Description: <p>Labile concentration of Scandium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663295.rdf
Name: Cd_Refractory
Units: picomole per liter (pmol/L)
Description: <p>Refractory concentration of Cadmium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663296.rdf
Name: La_Refractory
Units: picomole per liter (pmol/L)
Description: <p>Refractory concentration of Lanthanum</p>
http://lod.bco-dmo.org/id/dataset-parameter/663297.rdf
Name: Y_Refractory
Units: picomole per liter (pmol/L)
Description: <p>Refractory concentration of Yttrium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663298.rdf
Name: Pb_Refractory
Units: picomole per liter (pmol/L)
Description: <p>Refractory concentration of Lead</p>
http://lod.bco-dmo.org/id/dataset-parameter/663299.rdf
Name: Ba_Refractory
Units: picomole per liter (pmol/L)
Description: <p>Refractory concentration of Barium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663300.rdf
Name: Th_Refractory
Units: picomole per liter (pmol/L)
Description: <p>Refractory concentration of Thorium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663301.rdf
Name: Al_Refractory
Units: picomole per liter (pmol/L)
Description: <p>Refractory concentration of Aluminum</p>
http://lod.bco-dmo.org/id/dataset-parameter/663302.rdf
Name: Mn_Refractory
Units: picomole per liter (pmol/L)
Description: <p>Refractory concentration of Maganese</p>
http://lod.bco-dmo.org/id/dataset-parameter/663303.rdf
Name: Fe_Refractory
Units: picomole per liter (pmol/L)
Description: <p>Refractory concentration of Iron</p>
http://lod.bco-dmo.org/id/dataset-parameter/663304.rdf
Name: Co_Refractory
Units: picomole per liter (pmol/L)
Description: <p>Refractory concentration of Cobalt</p>
http://lod.bco-dmo.org/id/dataset-parameter/663305.rdf
Name: Cu_Refractory
Units: picomole per liter (pmol/L)
Description: <p>Refractory concentration of Copper</p>
http://lod.bco-dmo.org/id/dataset-parameter/663306.rdf
Name: Ni_Refractory
Units: picomole per liter (pmol/L)
Description: <p>Refractory concentration of Nickel</p>
http://lod.bco-dmo.org/id/dataset-parameter/663307.rdf
Name: P_Refractory
Units: picomole per liter (pmol/L)
Description: <p>Refractory concentration of Phosphorus</p>
http://lod.bco-dmo.org/id/dataset-parameter/663308.rdf
Name: Ti_Refractory
Units: picomole per liter (pmol/L)
Description: <p>Refractory concentration of Titanium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663309.rdf
Name: V_Refractory
Units: picomole per liter (pmol/L)
Description: <p>Refractory concentration of Vanadium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663310.rdf
Name: Zn_Refractory
Units: picomole per liter (pmol/L)
Description: <p>Refractory concentration of Zinc</p>
http://lod.bco-dmo.org/id/dataset-parameter/663311.rdf
Name: Sc_Refractory
Units: picomole per liter (pmol/L)
Description: <p>Refractory concentration of Scandium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663312.rdf
Name: Cd_Total
Units: picomole per liter (pmol/L)
Description: <p>Total particulate concentration (sum of Labile and Refractory) of Cadmium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663313.rdf
Name: La_Total
Units: picomole per liter (pmol/L)
Description: <p>Total particulate concentration (sum of Labile and Refractory) of Lanthanum</p>
http://lod.bco-dmo.org/id/dataset-parameter/663314.rdf
Name: Y_Total
Units: picomole per liter (pmol/L)
Description: <p>Total particulate concentration (sum of Labile and Refractory) of Yttrium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663315.rdf
Name: Pb_Total
Units: picomole per liter (pmol/L)
Description: <p>Total particulate concentration (sum of Labile and Refractory) of Lead</p>
http://lod.bco-dmo.org/id/dataset-parameter/663316.rdf
Name: Ba_Total
Units: picomole per liter (pmol/L)
Description: <p>Total particulate concentration (sum of Labile and Refractory) of Barium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663317.rdf
Name: Th_Total
Units: picomole per liter (pmol/L)
Description: <p>Total particulate concentration (sum of Labile and Refractory) of Thorium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663318.rdf
Name: Al_Total
Units: picomole per liter (pmol/L)
Description: <p>Total particulate concentration (sum of Labile and Refractory) of Aluminum</p>
http://lod.bco-dmo.org/id/dataset-parameter/663319.rdf
Name: Mn_Total
Units: picomole per liter (pmol/L)
Description: <p>Total particulate concentration (sum of Labile and Refractory) of Maganese</p>
http://lod.bco-dmo.org/id/dataset-parameter/663320.rdf
Name: Fe_Total
Units: picomole per liter (pmol/L)
Description: <p>Total particulate concentration (sum of Labile and Refractory) of Iron</p>
http://lod.bco-dmo.org/id/dataset-parameter/663321.rdf
Name: Co_Total
Units: picomole per liter (pmol/L)
Description: <p>Total particulate concentration (sum of Labile and Refractory) of Cobalt</p>
http://lod.bco-dmo.org/id/dataset-parameter/663322.rdf
Name: Cu_Total
Units: picomole per liter (pmol/L)
Description: <p>Total particulate concentration (sum of Labile and Refractory) of Copper</p>
http://lod.bco-dmo.org/id/dataset-parameter/663323.rdf
Name: Ni_Total
Units: picomole per liter (pmol/L)
Description: <p>Total particulate concentration (sum of Labile and Refractory) of Nickel</p>
http://lod.bco-dmo.org/id/dataset-parameter/663324.rdf
Name: P_Total
Units: picomole per liter (pmol/L)
Description: <p>Total particulate concentration (sum of Labile and Refractory) of Phosphorus</p>
http://lod.bco-dmo.org/id/dataset-parameter/663325.rdf
Name: Ti_Total
Units: picomole per liter (pmol/L)
Description: <p>Total particulate concentration (sum of Labile and Refractory) of Titanium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663326.rdf
Name: V_Total
Units: picomole per liter (pmol/L)
Description: <p>Total particulate concentration (sum of Labile and Refractory) of Vanadium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663327.rdf
Name: Zn_Total
Units: picomole per liter (pmol/L)
Description: <p>Total particulate concentration (sum of Labile and Refractory) of Zinc</p>
http://lod.bco-dmo.org/id/dataset-parameter/663328.rdf
Name: Sc_Total
Units: picomole per liter (pmol/L)
Description: <p>Total particulate concentration (sum of Labile and Refractory) of Scandium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663329.rdf
Name: Cd_LabileFlag
Units: unitless
Description: <p>Quality flag for Labile concentration of Cadmium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663330.rdf
Name: La_LabileFlag
Units: unitless
Description: <p>Quality flag for Labile concentration of Lanthanum</p>
http://lod.bco-dmo.org/id/dataset-parameter/663331.rdf
Name: Y_LabileFlag
Units: unitless
Description: <p>Quality flag for Labile concentration of Yttrium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663332.rdf
Name: Pb_LabileFlag
Units: unitless
Description: <p>Quality flag for Labile concentration of Lead</p>
http://lod.bco-dmo.org/id/dataset-parameter/663333.rdf
Name: Ba_LabileFlag
Units: unitless
Description: <p>Quality flag for Labile concentration of Barium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663334.rdf
Name: Th_LabileFlag
Units: unitless
Description: <p>Quality flag for Labile concentration of Thorium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663335.rdf
Name: Al_LabileFlag
Units: unitless
Description: <p>Quality flag for Labile concentration of Aluminum</p>
http://lod.bco-dmo.org/id/dataset-parameter/663336.rdf
Name: Mn_LabileFlag
Units: unitless
Description: <p>Quality flag for Labile concentration of Maganese</p>
http://lod.bco-dmo.org/id/dataset-parameter/663337.rdf
Name: Fe_LabileFlag
Units: unitless
Description: <p>Quality flag for Labile concentration of Iron</p>
http://lod.bco-dmo.org/id/dataset-parameter/663338.rdf
Name: Co_LabileFlag
Units: unitless
Description: <p>Quality flag for Labile concentration of Cobalt</p>
http://lod.bco-dmo.org/id/dataset-parameter/663339.rdf
Name: Cu_LabileFlag
Units: unitless
Description: <p>Quality flag for Labile concentration of Copper</p>
http://lod.bco-dmo.org/id/dataset-parameter/663340.rdf
Name: Ni_LabileFlag
Units: unitless
Description: <p>Quality flag for Labile concentration of Nickel</p>
http://lod.bco-dmo.org/id/dataset-parameter/663341.rdf
Name: P_LabileFlag
Units: unitless
Description: <p>Quality flag for Labile concentration of Phosphorus</p>
http://lod.bco-dmo.org/id/dataset-parameter/663342.rdf
Name: Ti_LabileFlag
Units: unitless
Description: <p>Quality flag for Labile concentration of Titanium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663343.rdf
Name: V_LabileFlag
Units: unitless
Description: <p>Quality flag for Labile concentration of Vanadium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663344.rdf
Name: Zn_LabileFlag
Units: unitless
Description: <p>Quality flag for Labile concentration of Zinc</p>
http://lod.bco-dmo.org/id/dataset-parameter/663345.rdf
Name: Sc_LabileFlag
Units: unitless
Description: <p>Quality flag for Labile concentration of Scandium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663346.rdf
Name: Cd_RefrFlag
Units: unitless
Description: <p>Quality flag for Refractory concentration of Cadmium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663347.rdf
Name: La_RefrFlag
Units: unitless
Description: <p>Quality flag for Refractory concentration of Lanthanum</p>
http://lod.bco-dmo.org/id/dataset-parameter/663348.rdf
Name: Y_RefrFlag
Units: unitless
Description: <p>Quality flag for Refractory concentration of Yttrium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663349.rdf
Name: Pb_RefrFlag
Units: unitless
Description: <p>Quality flag for Refractory concentration of Lead</p>
http://lod.bco-dmo.org/id/dataset-parameter/663350.rdf
Name: Ba_RefrFlag
Units: unitless
Description: <p>Quality flag for Refractory concentration of Barium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663351.rdf
Name: Th_RefrFlag
Units: unitless
Description: <p>Quality flag for Refractory concentration of Thorium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663352.rdf
Name: Al_RefrFlag
Units: unitless
Description: <p>Quality flag for Refractory concentration of Aluminum</p>
http://lod.bco-dmo.org/id/dataset-parameter/663353.rdf
Name: Mn_RefrFlag
Units: unitless
Description: <p>Quality flag for Refractory concentration of Maganese</p>
http://lod.bco-dmo.org/id/dataset-parameter/663354.rdf
Name: Fe_RefrFlag
Units: unitless
Description: <p>Quality flag for Refractory concentration of Iron</p>
http://lod.bco-dmo.org/id/dataset-parameter/663355.rdf
Name: Co_RefrFlag
Units: unitless
Description: <p>Quality flag for Refractory concentration of Cobalt</p>
http://lod.bco-dmo.org/id/dataset-parameter/663356.rdf
Name: Cu_RefrFlag
Units: unitless
Description: <p>Quality flag for Refractory concentration of Copper</p>
http://lod.bco-dmo.org/id/dataset-parameter/663357.rdf
Name: Ni_RefrFlag
Units: unitless
Description: <p>Quality flag for Refractory concentration of Nickel</p>
http://lod.bco-dmo.org/id/dataset-parameter/663358.rdf
Name: P_RefrFlag
Units: unitless
Description: <p>Quality flag for Refractory concentration of Phosphorus</p>
http://lod.bco-dmo.org/id/dataset-parameter/663359.rdf
Name: Ti_RefrFlag
Units: unitless
Description: <p>Quality flag for Refractory concentration of Titanium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663360.rdf
Name: V_RefrFlag
Units: unitless
Description: <p>Quality flag for Refractory concentration of Vanadium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663361.rdf
Name: Zn_RefrFlag
Units: unitless
Description: <p>Quality flag for Refractory concentration of Zinc</p>
http://lod.bco-dmo.org/id/dataset-parameter/663362.rdf
Name: Sc_RefrFlag
Units: unitless
Description: <p>Quality flag for Refractory concentration of Scandium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663363.rdf
Name: Cd_TotalFlag
Units: unitless
Description: <p>Quality flag for total particulate concentration (sum of Labile and Refractory) of Cadmium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663364.rdf
Name: La_TotalFlag
Units: unitless
Description: <p>Quality flag for total particulate concentration (sum of Labile and Refractory) of Lanthanum</p>
http://lod.bco-dmo.org/id/dataset-parameter/663365.rdf
Name: Y_TotalFlag
Units: unitless
Description: <p>Quality flag for total particulate concentration (sum of Labile and Refractory) of Yttrium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663366.rdf
Name: Pb_TotalFlag
Units: unitless
Description: <p>Quality flag for total particulate concentration (sum of Labile and Refractory) of Lead</p>
http://lod.bco-dmo.org/id/dataset-parameter/663367.rdf
Name: Ba_TotalFlag
Units: unitless
Description: <p>Quality flag for total particulate concentration (sum of Labile and Refractory) of Barium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663368.rdf
Name: Th_TotalFlag
Units: unitless
Description: <p>Quality flag for total particulate concentration (sum of Labile and Refractory) of Thorium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663369.rdf
Name: Al_TotalFlag
Units: unitless
Description: <p>Quality flag for total particulate concentration (sum of Labile and Refractory) of Aluminum</p>
http://lod.bco-dmo.org/id/dataset-parameter/663370.rdf
Name: Mn_TotalFlag
Units: unitless
Description: <p>Quality flag for total particulate concentration (sum of Labile and Refractory) of Maganese</p>
http://lod.bco-dmo.org/id/dataset-parameter/663371.rdf
Name: Fe_TotalFlag
Units: unitless
Description: <p>Quality flag for total particulate concentration (sum of Labile and Refractory) of Iron</p>
http://lod.bco-dmo.org/id/dataset-parameter/663372.rdf
Name: Co_TotalFlag
Units: unitless
Description: <p>Quality flag for total particulate concentration (sum of Labile and Refractory) of Cobalt</p>
http://lod.bco-dmo.org/id/dataset-parameter/663373.rdf
Name: Cu_TotalFlag
Units: unitless
Description: <p>Quality flag for total particulate concentration (sum of Labile and Refractory) of Copper</p>
http://lod.bco-dmo.org/id/dataset-parameter/663374.rdf
Name: Ni_TotalFlag
Units: unitless
Description: <p>Quality flag for total particulate concentration (sum of Labile and Refractory) of Nickel</p>
http://lod.bco-dmo.org/id/dataset-parameter/663375.rdf
Name: P_TotalFlag
Units: unitless
Description: <p>Quality flag for total particulate concentration (sum of Labile and Refractory) of Phosphorus</p>
http://lod.bco-dmo.org/id/dataset-parameter/663376.rdf
Name: Ti_TotalFlag
Units: unitless
Description: <p>Quality flag for total particulate concentration (sum of Labile and Refractory) of Titanium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663377.rdf
Name: V_TotalFlag
Units: unitless
Description: <p>Quality flag for total particulate concentration (sum of Labile and Refractory) of Vanadium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663378.rdf
Name: Zn_TotalFlag
Units: unitless
Description: <p>Quality flag for total particulate concentration (sum of Labile and Refractory) of Zinc</p>
http://lod.bco-dmo.org/id/dataset-parameter/663379.rdf
Name: Sc_TotalFlag
Units: unitless
Description: <p>Quality flag for total particulate concentration (sum of Labile and Refractory) of Scandium</p>
http://lod.bco-dmo.org/id/dataset-parameter/663380.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>Date/Time of sampling at station (UTC) ISO formatted</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
28888
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/e8b92d0e-de3e-4a69-bf00-3a57399f0b9e/content
download
28888
https://darchive.mblwhoilibrary.org/server/api/core/bitstreams/c8e661d1-ebc3-4ae5-ab32-1e9751531579/content
download
https://doi.org/10.26008/1912/bco-dmo.663183.1
download
onLine
dataset
<p><strong>Total, refractory, and labile particulate element concentrations via ICPMS:</strong><br />
Labile and total suspended particulate trace elements concentrations are reported for: Al, Ba, Cd, Co, Cu, Fe, La, Mn, Ni, P, Pb, Sc, Th, Ti, V, Y, Zn. Concentrations of the labile fraction of these particulate elements are indicated as element names followed by the suffix ‘-Labile’, refractory portions are indicated with the suffix ‘-Refractory’, and concentrations of total particulate elements (the sum of labile and refractory) are followed by ‘-Total’. Concentrations are reported in units of picomoles per liter (pmol/L).</p>
<p><strong>Sampling Methodology:</strong><br />
Trace metal-clean seawater samples were collected using a sampling system consisting of Teflon-coated GO-Flo bottles and following methods described in Bruland et al (1979). Additional samples were collected from surface waters (~2 meters) using a towed ‘fish’ deployed by Ken Bruland's lab. Water collected with GO-Flo bottles and the ‘fish’ was transferred into acid-washed 4-liter (L) LDPE carboys for off-line filtration.</p>
<p>All filtration was conducted in a HEPA-filtered 'bubble' (temporary clean room). A filter holder containing a 25-millimeter (mm) diameter Pall Supor 0.4-micrometer (um) polyethersulfone membrane was attached to the opening at the carboy top. Carboys were pressurized with 0.2‐um filtered air and inverted during filtration to ensure that all particles were captured on the membrane. Filtrate was collected to determine the volume of seawater filtered: an average of 2.1L was filtered through each membrane. After filtration, membranes were folded into quarters, placed in 1.7-milliliter (mL) polypropylene vials, and stored at -20 degrees Celsius until analysis.</p>
<p><strong>Analytical Methodology:</strong><br />
All digestion steps were performed in a Class-100 clean room using standard clean techniques. Filters were sequentially digested, first following the protocol of Berger et al. (2008) to obtain labile particulate concentrations and then digested using a 4M HCl, 4M HNO3, and 4M HF mixture as described in Ohnemus et al. (2014) to obtain refractory particulate element concentrations.</p>
<p>For the labile particulate leach, a 1-milliliter solution of 25% Optima-grade acetic acid and 0.02 M hydroxylamine was added to the filter stored in a 1.7 mL polypropylene vial. Following the recommendation of Berger et al. (2008), the solution was heated to 95 degrees C in a water bath for 10 minutes and then allowed to cool to room temperature. The filter was in contact with the acetic acid leach for a total of two hours, after which the filter was removed from the polypropylene vial and placed in an acid-cleaned 22-mL PFA vial. The acetic acid/hydroxylamine leachate was centrifuged at 14,000 rpm for 10 minutes to sediment all particles. Without disturbing particles on the bottom of the tube, approximately 0.8 mL of leachate was transferred into an acid-cleaned 7 mL PFA digestion vial. Optima-grade HNO3 was added (100 uL) to the digestion vial, which was subsequently heated uncapped at 110 degrees C to near dryness. Vial contents were redissolved with 2% HNO3 (Optima grade).</p>
<p>Refractory particulate metals were determined by subsequent digestion of the filter. Two milliliters of a solution containing 4M HCl, 4M HNO3, and 4M HF (all Optima grade) was added to the filter which was placed in a cleaned 22-mL PFA vial. The vial was tightly capped and heated to 110°C for 4 hours. This procedure has been determined to be adequate for digestion of all particulate material, while allowing the Supor filter to remain intact (Ohnemus et al. 2014). Following heating, the acid solution in the bomb was poured into a second PFA vial, leaving the filter piece behind. To ensure complete transfer of acid, the bombs were thoroughly rinsed with 3 × 0.5 mL aliquots of ultrapure water which were poured into the secondary vial. The secondary vial was then heated to dryness and the contents redissolved with 1 mL of a 50% Optima-grade HNO3 + 15% Optima-grade H2O2 (v/v of concentrated reagents) solution. This solution was again dried down and the contents redissolved with 2% HNO3.</p>
<p>All digests were analyzed using a Finnigan-MAT Element2 HR-ICP-MS at the University of Maine following the protocols outlined in Twining et al. (2011). The instrument is equipped with a cyclonic nebulizer, an autosampler contained under a HEPA filter, and nickel cones. Ba, Cd, La, Th, and Y were analyzed in low-resolution mode, while the remaining isotopes were analyzed in medium-resolution mode.</p>
<p>Quantification was performed by external calibration, and In-115 was used as an internal standard to correct for variations in instrumental sensitivity during analyses. Cs-133, spiked during the initial sample leaches, was used as a process recovery monitor, but no samples were discarded or corrected using the Cs recoveries, as typical Cs recoveries were 90-110%. Certified reference materials were digested alongside refractory sample digests. Average recoveries for each element are given in the attached supplemental file "CRMs.png".</p>
Specified by the Principal Investigator(s)
<p>All ICP-MS elemental concentration data were normalized to an In-115 internal standard and quantified using external standard curves. After accounting for sample dilutions due to acid digestion steps, quantities of each element per filter (pmol/filter) were calculated for each analytical run. The contribution of the ‘process blank’ (measured as the elements contained in an acid-washed filter through which 0.2-um filtered water was passed during the cruise) was then subtracted. Process blanks were pooled from across the cruise section. Separate process blanks were calculated for the labile (acetic acid/hydroxylamine) and refractory (HCl/HNO3/HF) digestions. The median process blanks for each digestion scheme and each element are given in the attached supplemental file "Blanks_and_DetectionLimits.png".</p>
<p>Following process blank correction, element concentrations (per volume of water filtered) were calculated by dividing the determined pmol/filter by the volume of water passed through each filter.</p>
<p>Detection limits are calculated as 3 times the standard deviation of the process blanks for the relevant digestion procedure after pooling of process blanks from across the transect.</p>
<p>Total element concentrations are calculated as the sum of the labile and refractory portions. Total concentrations are not reported if either the labile or refractory concentrations are below detection limits.</p>
<p><strong>Description of data quality flags:</strong></p>
<p>The standard Ocean Data View flags were used:</p>
<p>1: Good Value: Used when replicate samples were analyzed for a particular concentration.</p>
<p>2: Probably Good Value: Used when the reported value reflects analysis of a single replicate.</p>
<p>3: Probably Bad Value: Used when a value appears abnormally high or low (oceanographically inconsistent) based on adjacent depths or typical profile variability and shape using the context of relevant nearby stations.</p>
<p>6: Value Below Detection Limit: Used when value is below the detection limit for that given element. Empty values are reported rather than zero or a detection limit value.</p>
Specified by the Principal Investigator(s)
- reformatted column names to comply with BCO-DMO standards.
- filled in blank cells with "nd"
- added the DateTime column "ISO_DateTime_UTC"
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Towed fish
Towed fish
PI Supplied Instrument Name: Towed fish PI Supplied Instrument Description:Used to collect surface seawater samples Instrument Name: GeoFish Towed near-Surface Sampler Instrument Short Name:GeoFish Instrument Description: The GeoFish towed sampler is a custom designed near surface (2 meters or less) sampling system for the collection of trace metal clean seawater. It consists of a PVC encapsulated lead weighted torpedo and separate PVC depressor vane supporting the intake utilizing all PFA Teflon tubing connected to a deck mounted, air-driven, PFA Teflon dual-diaphragm pump which provides trace-metal clean seawater at up to 3.7L/min. The GeoFish is towed at up to 13kts off to the side of the vessel outside of the ship's wake to avoid possible contamination from the ship's hull. It was developed by Geoffrey Smith and Ken Bruland (University of California, Santa Cruz). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/31/
Teflon-coated GO-Flo Bottle
Teflon-coated GO-Flo Bottle
PI Supplied Instrument Name: Teflon-coated GO-Flo Bottle PI Supplied Instrument Description:Used to collect trace metal-clean seawater samples Instrument Name: GO-FLO Teflon Trace Metal Bottle Instrument Short Name:GO-FLO Teflon TM Instrument Description: GO-FLO Teflon-lined Trace Metal free sampling bottles are used for collecting water samples for trace metal, nutrient and pigment analysis. The GO-FLO sampling bottle is designed specifically to avoid sample contamination at the surface, internal spring contamination, loss of sample on deck (internal seals), and exchange of water from different depths. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/30/
Cruise: MV1405
MV1405
R/V Melville
Community Standard Description
International Council for the Exploration of the Sea
R/V Melville
vessel
MV1405
Kenneth W. Bruland
University of California-Santa Cruz
R/V Melville
Community Standard Description
International Council for the Exploration of the Sea
R/V Melville
vessel