http://lod.bco-dmo.org/id/dataset/663707
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2016-11-01
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Nutrient concentrations, microbiology (Vibrio abundance), trace metals, and environmental conditions from collection sites in the Florida Keys National Marine Sanctuary, 2014-2016
2016-11-03
publication
2016-11-03
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-06-03
publication
https://doi.org/10.26008/1912/bco-dmo.663707.1
Erin K. Lipp
University of Georgia
principalInvestigator
William M. Landing
Florida State University EOAS
principalInvestigator
Elizabeth Ottesen
University of Georgia
principalInvestigator
Michael Wetz
Texas A&M University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Lipp, E., Landing, W., Ottesen, E., Wetz, M. (2020) Nutrient concentrations, microbiology (Vibrio abundance), trace metals, and environmental conditions from collection sites in the Florida Keys National Marine Sanctuary, 2014-2016. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2016-11-03 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.663707.1 [access date]
Vibrio abundance and nutrients Dataset Description: <p>Nutrient concentrations, microbiology (Vibrio abundance), trace metals, and environmental conditions from collection sites in the Florida Keys National Marine Sanctuary, 2014-2016.</p> Methods and Sampling: <p>Samples were collected offshore from Alligator Reef (2014) and Looe Key Reef (2015-2016). Measurements included temperature, salinity, pH, abundance of Vibrio, chlorophyll-a, DOC, TDN, DON, NH4, NO3, NO2, Orthophosphate ,SiO4, and dFe.</p>
<p>Dissolved organic carbon (DOC) and total dissolved nitrogen (TDN) were determined using the filtrate of water samples that were passed through precombusted 25 mm GF/F filters and stored frozen (-20C) until analysis.&nbsp; Samples were subsequently analyzed using the High Temperature Catalytic Oxidation method on a Shimadzu TOC-Vs analyzer with nitrogen module.&nbsp; Standard curves were run twice daily using a DIW blank and five concentrations of either acid potassium phthalate solution or potassium nitrate for DOC and TDN, respectively.&nbsp; Three to five subsamples were taken from each standard and water sample and injected in sequence.&nbsp; Reagent grade glucosamine was used as a laboratory check standard and inserted throughout each run, as were Certified Reference Material Program (CRMP) deep-water standards of known DOC/TDN concentration.&nbsp;</p>
<p>Nutrients data went through internal lab QAQC process. BDL means below detection limit. The method detection limit (MDL) was determined using 9 samples on two different runs and correct student-T value.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1357423 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1357423
onGoing
Erin K. Lipp
University of Georgia
706-583-8138
150 East Green St.
Athens
GA
30605
United States
elipp@uga.edu
pointOfContact
William M. Landing
Florida State University EOAS
850-644-6037
Department of Earth, Ocean, and Atmospheric Science 117 N. Woodward Avenue
Tallahassee
FL
32306-4320
USA
wlanding@fsu.edu
pointOfContact
Elizabeth Ottesen
University of Georgia
706-542-9582
120 Cedar St.
Athens
GA
30605
United States
ottesen@uga.edu
pointOfContact
Michael Wetz
Texas A&M University
361-825-2132
6300 Ocean Drive
Corpus Christi
TX
78412
United States
michael.wetz@tamucc.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
sample
date
time_start_local
depth
location
station_notes
lat
lon
temp
sal
pH
Vibrio
Chl_a
DOC
TDN
DON
NH4
NO3
NO2
Orthophosphate
SiO4
dFe
chemoluminescence gas analyzer
theme
None, User defined
sample identification
date
time_start_local
depth
site
comments
latitude
longitude
water temperature at measurement depth
salinity
pH
No BCO-DMO term
chlorophyll a
dissolved organic Carbon
Total Dissolved Nitrogen
Dissolved Organic Nitrogen
Ammonium
Nitrate
Nitrite
reactive phosphorus (PO4)
Silicate (reactive silica)
Iron
featureType
BCO-DMO Standard Parameters
Gas Analyzer
plate reader
instrument
BCO-DMO Standard Instruments
Lipp_2014-16
service
Deployment Activity
Florida Keys National Marine Sanctuary
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Vibrio as a model microbe for opportunistic heterotrophic response to Saharan dust deposition events in marine waters
https://osprey.bco-dmo.org/project/553933
Vibrio as a model microbe for opportunistic heterotrophic response to Saharan dust deposition events in marine waters
<p><em>Description from NSF award abstract:</em><br />
Dust and mineral aerosols are a significant source of micro and macronutrients to oligotrophic ocean surface waters. Evidence is growing that heterotrophic microbes may play key roles in processing deposited minerals and nutrients. Yet it is not known which components of dust stimulate the heterotrophic bacteria, which cellular mechanisms are responsible for the utilization of those components and how the activity of these bacteria affect the availability and utilization of dust-derived minerals and nutrients by marine autotrophs. Knowledge of these factors is key to understanding how dust deposition impacts carbon cycles and for predicting the response of tropical oceans to future changes in the frequency and intensity of dust deposition events. The objective of this project is to examine the specific effects of aeolian dust on heterotrophic microbes in a tropical marine system under controlled conditions. The central hypothesis is that in oligotrophic tropical systems numerically minor opportunistic bacteria are the first responders to influx of dust constituents and respond primarily by rapidly accessing soluble trace metals and limiting nutrients that are deposited with Saharan dust. The project will focus on two specific aims: 1) Quantify changes in community structure, composition and transcriptional activity among marine microbial populations upon exposure to dust, and 2) Identify key components in Saharan dust aerosols that stimulate or repress growth and/or activity in Vibrio, a model opportunistic marine heterotrophic group. The study will use a series of controlled experiments designed to identify and quantify heterotrophic microbial response to dust deposition events using both natural communities and model bacteria (Vibrio) through metagenomics, transcriptomics and atmospheric and marine biogeochemical techniques. This innovative approach will identify the most critical (reactive) components leached from dust aerosols on the microbial community as well as elucidate potential mechanisms of response.</p>
<p>There is great interest in the biological response to dust aerosols given its potentially large influence on biogeochemical cycling, but there has been relatively little work that has addressed the mechanisms of response (especially among the heterotrophic microbial fraction) or identified the relative importance of specific constituents of dust aerosols. A detailed framework for microbial response (focusing on opportunistic heterotrophs) will facilitate efforts to link autotrophic and heterotrophic processing. This contribution is significant because it will provide one of the first end-to-end (chemistry to physiology to ecology) mechanistic pathways for marine biological response to desert dust aerosols.</p>
Vibrio-dust deposition
largerWorkCitation
project
eng; USA
biota
oceans
Florida Keys National Marine Sanctuary
-81.41115
-80.68487
24.54322
24.74287
2014-07-22
2016-07-31
Florida Keys, FL, USA
0
BCO-DMO catalogue of parameters from Nutrient concentrations, microbiology (Vibrio abundance), trace metals, and environmental conditions from collection sites in the Florida Keys National Marine Sanctuary, 2014-2016
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/663868.rdf
Name: sample
Units: unitless
Description: <p>sample identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/663869.rdf
Name: date
Units: year-month-day
Description: <p>sampling date formatted as yyyy-mm-dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/663870.rdf
Name: time_start_local
Units: hours:minutes
Description: <p>local start time formatted as HH:MM</p>
http://lod.bco-dmo.org/id/dataset-parameter/663871.rdf
Name: depth
Units: feet
Description: <p>depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/663872.rdf
Name: location
Units: unitless
Description: <p>sample collection location</p>
http://lod.bco-dmo.org/id/dataset-parameter/663873.rdf
Name: station_notes
Units: unitless
Description: <p>comments pertaining to the station</p>
http://lod.bco-dmo.org/id/dataset-parameter/663874.rdf
Name: lat
Units: decimal degrees
Description: <p>latitude; north is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/663875.rdf
Name: lon
Units: decimal degrees
Description: <p>longitude; east is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/663876.rdf
Name: temp
Units: degrees Celsius
Description: <p>temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/663877.rdf
Name: sal
Units: Practical Salinity Units (PSU)
Description: <p>salinity</p>
http://lod.bco-dmo.org/id/dataset-parameter/663878.rdf
Name: pH
Units: unitless; pH scale
Description: <p>pH: The measure of the acidity or basicity of an aqueous solution</p>
http://lod.bco-dmo.org/id/dataset-parameter/663879.rdf
Name: Vibrio
Units: colony forming units/milliliter (CFU/ml)
Description: <p>Vibrio colony concentration. CFU/ml determined by spread on TCBS agar in triplicate for each experimental replicate and counting green and yellow colonies after 18 - 24 incubation at 30 C. limit of detection was 3.3 CFU/ml (determined using 100 ul spread volume in triplicate); 0.0 = below detection limit.</p>
http://lod.bco-dmo.org/id/dataset-parameter/663880.rdf
Name: Chl_a
Units: micrograms/liter (ug/ml)
Description: <p>Chlorophyll-a concentration determined by acetone freeze thaw using EPA method 445.0 (non-acidification); bdl for below detection limit.</p>
http://lod.bco-dmo.org/id/dataset-parameter/663881.rdf
Name: DOC
Units: micromoles/liter (ug/ml)
Description: <p>Dissolved organic carbon concentration: determined using oxidative high temperature combustion-infrared analysis. MDL is 11.16 micromol/L.</p>
http://lod.bco-dmo.org/id/dataset-parameter/663882.rdf
Name: TDN
Units: micromoles/liter (ug/ml)
Description: <p>Total dissolved nitrogen concentration determined using oxidative high temperature combustion-infrared analysis.</p>
http://lod.bco-dmo.org/id/dataset-parameter/663883.rdf
Name: DON
Units: micromoles/liter (ug/ml)
Description: <p>Dissolved organic nitrogen concentration; MDL is 5.38 micromol/L (determined using 9 samples on two different runs and correct student-T value). bdl = below detection limit.</p>
http://lod.bco-dmo.org/id/dataset-parameter/663884.rdf
Name: NH4
Units: micromoles/liter (ug/ml)
Description: <p>Ammonium concentration determined by the automated phenate method 4500-NH3G. (20th Edition Std. Meth.); MDL is 0.3 micro gram/L (determined using 9 samples on two different runs and correct student-T value). bdl = below detection limit.</p>
http://lod.bco-dmo.org/id/dataset-parameter/663885.rdf
Name: NO3
Units: micromoles/liter (ug/ml)
Description: <p>Nitrate concentration determined by the automated cadmium reduction method 4500-NO3- F (20th Edition Std. Meth.); MDL is 0.3 micro gram/L (determined using 9 samples on two different runs and correct student-T value). bdl = below detection limit.</p>
http://lod.bco-dmo.org/id/dataset-parameter/663886.rdf
Name: NO2
Units: micromoles/liter (ug/ml)
Description: <p>Nitrite concentration determined as with Nitrate without running the sample through a cadmium column (20th Edition Std. Meth.); MDL is 0.1 micro gram/L (determined using 9 samples on two different runs and correct student-T value). bdl = below detection limit.</p>
http://lod.bco-dmo.org/id/dataset-parameter/663887.rdf
Name: Orthophosphate
Units: micromoles/liter (ug/ml)
Description: <p>Orthophosphate concentration determined by the automated ascorbic acid reduction method 4500-P F. (20th Edition Std. Meth.); MDL is 0.2 micro gram/L (determined using 9 samples on two different runs and correct student-T value). bdl = below detection limit.</p>
http://lod.bco-dmo.org/id/dataset-parameter/663888.rdf
Name: SiO4
Units: micromoles/liter (ug/ml)
Description: <p>Silicate concentration determined by the automated molybdate-reactive silica method 4500-SiO2 E. (20th Edition Std. Meth.); MDL is 0.3 micro gram/L (determined using 9 samples on two different runs and correct student-T value). bdl = below detection limit.</p>
http://lod.bco-dmo.org/id/dataset-parameter/663889.rdf
Name: dFe
Units: nanomoles/liter (ug/ml)
Description: <p>dissolved iron concentration determined in the 0.2 um filtered fraction using ICP-MSas described in Milne et al. 2010. Analytica Chimera Acta 665: 200-207</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
25241
https://darchive.mblwhoilibrary.org/bitstream/1912/25817/1/dataset-663707_field-collections-all-data__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.663707.1
download
onLine
dataset
<p>Samples were collected offshore from Alligator Reef (2014) and Looe Key Reef (2015-2016). Measurements included temperature, salinity, pH, abundance of Vibrio, chlorophyll-a, DOC, TDN, DON, NH4, NO3, NO2, Orthophosphate ,SiO4, and dFe.</p>
<p>Dissolved organic carbon (DOC) and total dissolved nitrogen (TDN) were determined using the filtrate of water samples that were passed through precombusted 25 mm GF/F filters and stored frozen (-20C) until analysis.&nbsp; Samples were subsequently analyzed using the High Temperature Catalytic Oxidation method on a Shimadzu TOC-Vs analyzer with nitrogen module.&nbsp; Standard curves were run twice daily using a DIW blank and five concentrations of either acid potassium phthalate solution or potassium nitrate for DOC and TDN, respectively.&nbsp; Three to five subsamples were taken from each standard and water sample and injected in sequence.&nbsp; Reagent grade glucosamine was used as a laboratory check standard and inserted throughout each run, as were Certified Reference Material Program (CRMP) deep-water standards of known DOC/TDN concentration.&nbsp;</p>
<p>Nutrients data went through internal lab QAQC process. BDL means below detection limit. The method detection limit (MDL) was determined using 9 samples on two different runs and correct student-T value.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- nd (no data) was entered into all blank cells<br />
- reduced number of significant digits of Vibrio and nutrient values to 2 digits to right of decimal and lat/lon to 5 digits.<br />
- re-formatted date from m/d/yyyy to yyyy-mm-dd<br />
- replaced spaces with underscores</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
chemoluminescence gas analyzer
chemoluminescence gas analyzer
PI Supplied Instrument Name: chemoluminescence gas analyzer PI Supplied Instrument Description:To measure dissolved organic nitrogen Instrument Name: Gas Analyzer Instrument Short Name:Gas Analyzer Instrument Description: Gas Analyzers - Instruments for determining the qualitative and quantitative composition of gas mixtures.
PI Supplied Instrument Name: PI Supplied Instrument Description:To measure colony counts Instrument Name: plate reader Instrument Short Name: Instrument Description: Plate readers (also known as microplate readers) are laboratory instruments designed to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. Sample reactions can be assayed in 6-1536 well format microtiter plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 uL per well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µL per well. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. From: http://en.wikipedia.org/wiki/Plate_reader, 2014-09-0-23.
Deployment: Lipp_2014-16
Lipp_2014-16
Florida Keys National Marine Sanctuary
shoreside
Lipp_2014-16
Erin K. Lipp
University of Georgia
Florida Keys National Marine Sanctuary
shoreside