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            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset/663860.rdf" xlink:actuate="onRequest">Carbon geochemistry of Santa Elena (Costa Rica) ultramafics and bulk rock carbonate samples from expeditions 149, 173, and 209 on R/V JOIDES Resolution in the Iberia Abyssal Plain, Iberian Margin, and Mid-Atlantic Ridge from 1993-2003</gmx:Anchor>
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            <gco:CharacterString>Cite this dataset as: Schwarzenbach, E. (2016) Carbon geochemistry of Santa Elena (Costa Rica) ultramafics and bulk rock carbonate samples from expeditions 149, 173, and 209 on R/V JOIDES Resolution in the Iberia Abyssal Plain, Iberian Margin, and Mid-Atlantic Ridge from 1993-2003. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 03 November 2016) Version Date 2016-11-03 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/663860 [access date]</gco:CharacterString>
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        <gco:CharacterString>Carbon geochemistry of the Santa Elena (Costa Rica) ultramafics and bulk rock carbonate samples Dataset Description: &amp;lt;p&amp;gt;Carbon geochemistry of the Santa Elena (Costa Rica) ultramafics and bulk rock carbonate samples.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Related publications:&amp;lt;br /&amp;gt;
Schwarzenbach EM, Gill BC, Gazel E, Madrigal P (2016) Sulfur and carbon geochemistry of the Santa Elena peridotites: Comparing oceanic and continental processes during peridotite alteration. Lithos 252–253:92-108. doi:&amp;lt;a href=&amp;quot;http://dx.doi.org/10.1016/j.lithos.2016.02.017&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;10.1016/j.lithos.2016.02.017&amp;lt;/a&amp;gt;&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Santa Elena samples:&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
&amp;lt;em&amp;gt;Sample preparation and mineralogy:&amp;amp;nbsp;&amp;lt;/em&amp;gt;Ultramafic basement rocks were sampled throughout the Santa Elena Ophiolite. Carbonate deposits associated with Mg-HCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;&amp;amp;nbsp;and Ca-OH springs in the eastern part of the ophiolite were sampled at various locations within riverbeds. All analytical measurements and sample preparation of the Santa Elena peridotites and carbonates were performed at the Department of Geosciences at Virginia Tech. The carbonate samples were prepared by drilling out individual depositional layers of carbonates to reveal heterogeneities within different layers or crushed with an agate mortar for bulk rock analysis. Carbonate precipitates from the rivers were first dried at 40 degrees&amp;amp;nbsp;C in the oven. Before sample analyses all samples were homogenized by hand with the agate mortar. The mineralogy of the carbonates was then determined by X-ray diffraction on a Rigaku MiniFlex XRD using the powder diffraction analysis package PDXL.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Bulk rock powders were prepared for all the ultramafic rocks. To remove contamination from weathering, the outermost 1-2 cm of the rock samples were cut away. The samples were then cleaned in an ultrasonic bath prior to powdering them with a shatter box using an alumina dish. These bulk rock powders were subsequently analyzed for their carbon and sulfur geochemistry.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Carbon geochemistry:&amp;amp;nbsp;&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
Carbon in these systems is typically detected as inorganic (e.g. carbonate carbon) or organic carbon. Organic carbon has also been referred to as reduced carbon or non-carbonate carbon. Here we refer to it as total organic carbon (TOC) even though the presence of traces of graphite and other reduced carbon species cannot entirely be excluded. Total carbon (TC) and total inorganic carbon (TIC) contents, d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C of TC, d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C of TOC and d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C and d&amp;lt;sup&amp;gt;18&amp;lt;/sup&amp;gt;O of TIC were analyzed on the basement rocks, while only d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C and d&amp;lt;sup&amp;gt;18&amp;lt;/sup&amp;gt;O of TIC were determined for the carbonate deposits and precipitates.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;TC contents, d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C&amp;lt;sub&amp;gt;TC&amp;lt;/sub&amp;gt;&amp;amp;nbsp;and d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C&amp;lt;sub&amp;gt;TOC&amp;lt;/sub&amp;gt;&amp;amp;nbsp;were determined on a Vario ISOTOPE elemental analyzer (EA) coupled to an Isoprime 100 isotope ratio mass spectrometer (IRMS). For analyses of d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C&amp;lt;sub&amp;gt;TOC&amp;lt;/sub&amp;gt;, bulk rock samples were reacted for three days with 3N HCl to remove all acid soluble carbon. They were then rinsed with H&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;O, dried at 40 degrees&amp;amp;nbsp;C in the oven and homogenized in the agate mortar. TC and TOC isotope values are reported in the standard delta&amp;amp;nbsp;notation relative to the Vienna-Pee Dee Belemnite (V-PDB) standard and calibrated to this scale using international [IAEA-CH-6 (sucrose; d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C = -10.449‰) and IAEA-CH-7 (polyethylene; d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C = -32.151‰)] and commercial standards [Elemental Microanalysis wheat flour; d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C = -27.21‰]. TOC was calculated from the difference between TC and TIC and errors are within 10% of the TOC contents. %TIC was calculated as TIC/TC*100. Reproducibility of d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C&amp;lt;sub&amp;gt;TC&amp;lt;/sub&amp;gt;&amp;amp;nbsp;and d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C&amp;lt;sub&amp;gt;TOC&amp;lt;/sub&amp;gt;&amp;amp;nbsp;is better than 0.1‰.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;The d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C&amp;lt;sub&amp;gt;TIC&amp;lt;/sub&amp;gt;&amp;amp;nbsp;and d&amp;lt;sup&amp;gt;18&amp;lt;/sup&amp;gt;O&amp;lt;sub&amp;gt;TIC&amp;lt;/sub&amp;gt;&amp;amp;nbsp;and TIC contents were analyzed on a MultiFlowGeo headspace sampler attached to an Isoprime 100 IRMS. Samples were prepared in septum vials, flushed with helium and acidified with phosphoric acid. Samples were then reacted for at least 3 hours at room temperature if no magnesite was present (as determined by XRD analysis). Magnesite-bearing samples were reacted for 72 hours at 90 degrees&amp;amp;nbsp;C. Carbon and oxygen isotope values are reported in the standard delta-notation relative to the Vienna-Pee Dee Belemnite (V-PDB) standard and calibrated to this scale using the international standards IAEA-CO-1 (marble; d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C = +2.492‰, d&amp;lt;sup&amp;gt;18&amp;lt;/sup&amp;gt;O = -2.4‰), IAEA-CO-9 (BaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;; d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C = -47.321‰, d&amp;lt;sup&amp;gt;18&amp;lt;/sup&amp;gt;O = -15.6‰) and NBS18 (calcite, d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C = -5.014‰, d&amp;lt;sup&amp;gt;18&amp;lt;/sup&amp;gt;O = -23.2‰). Reproducibility for the analysis of the samples was better than +/-&amp;amp;nbsp;0.07‰ for d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C and better than +/-&amp;amp;nbsp;0.3‰ for d&amp;lt;sup&amp;gt;18&amp;lt;/sup&amp;gt;O. Reproducibility of TIC contents is typically better than 5%.&amp;lt;/p&amp;gt;</gco:CharacterString>
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(1) coordinate, integrate, support, and extend the research associated with four major programs—Juan de Fuca Ridge flank (JdF), South Pacific Gyre (SPG), North Pond (NP), and Dorado Outcrop (DO)—and other field sites;
(2) make substantial investments of resources to support field, laboratory, analytical, and modeling studies of the deep subseafloor ecosystems;
(3) facilitate and encourage synthesis and thematic understanding of submarine microbiological processes, through funding of scientific and technical activities, coordination and hosting of meetings and workshops, and support of (mostly junior) researchers and graduate students; and
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Note: Katrina Edwards was a former PI of C-DEBI; James Cowen is a former co-PI.
Data Management:
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Serpentinization – the hydrothermal alteration of ultramafic rocks – is a unique mineralogical process that results in H2 and CH4 – rich fluids that can support microbial communities. Using a combination of petrographic observations, bulk rock and &lt;em&gt;in-situ&lt;/em&gt; sulfur isotope signatures of variably serpentinized peridotites from four different geotectonic environments we provide new constraints on the factors that support microbial activity. The studied samples overall suggest that redox conditions reflected by the presence of pyrite and pyrrhotite represent the fluid chemistry that favors microbial activity. This fluid chemistry, i.e., the prevailing hydrogen and sulfur fugacities of the fluid, are thereby correlated to high water-rock ratios and increased incorporation of seawater-derived species such as sulfate and carbonate. These species serve as major energy sources of microbial activity. In contrast, highly reducing conditions and limited fluid input limits or even prevents microbial activity within serpentinites due to insufficient availability of these species. Interaction with carbonate and sulfate-bearing meteoric waters is likely an essential process that supports microbial activity in continental serpentinization environments. Overall, this study shows that apart from fluid temperatures being within the limits of life, the fluid chemistry (redox conditions and availability of e.g. carbonate and sulfate) are the primary factors that control the presence or absence of microbial communities within serpentinizing peridotites. This study highlights the importance of combining bulk rock and &lt;em&gt;in-situ&lt;/em&gt; stable isotope data with petrographic and mineralogical observations in order to better constraint the presence of microbial communities within the subsurface of peridotite-hosted hydrothermal systems.&lt;/p&gt;
&lt;p&gt;Note: This project was funded by a &lt;a href=&quot;http://www.darkenergybiosphere.org/research-activities/research-support/research-grants/&quot; target=&quot;_blank&quot;&gt;C-DEBI Research Grant&lt;/a&gt;.&lt;/p&gt;</gco:CharacterString>
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	Name: sample_group
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	Description: &lt;p&gt;Sample group/type&lt;/p&gt; 
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	Name: sample_name
	Units: unitless
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http://lod.bco-dmo.org/id/dataset-parameter/663899.rdf
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	Description: &lt;p&gt;Sample description&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/663900.rdf
	Name: TC
	Units: percent Carbon (% C)
	Description: &lt;p&gt;Total carbon contents&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/663901.rdf
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	Units: percent Carbon (% C)
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http://lod.bco-dmo.org/id/dataset-parameter/663902.rdf
	Name: TIC
	Units: percent inorganic Carbon (% C)
	Description: &lt;p&gt;Total inorganic carbon contents&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/663903.rdf
	Name: TIC_err
	Units: percent inorganic Carbon (% C)
	Description: &lt;p&gt;Error of TIC&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/663904.rdf
	Name: TOC
	Units: parts per million (ppm)
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http://lod.bco-dmo.org/id/dataset-parameter/663905.rdf
	Name: pcnt_TIC
	Units: percent TIC (% TIC)
	Description: &lt;p&gt;Percent TIC&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/663906.rdf
	Name: del13C_TC
	Units: per mille
	Description: &lt;p&gt;Delta 13C of total carbon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/663907.rdf
	Name: del13C_TC_err
	Units: per mille
	Description: &lt;p&gt;Error of del13C_TC&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/663908.rdf
	Name: del13C_TIC
	Units: per mille
	Description: &lt;p&gt;Delta 13C of total inorganic carbon&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/663909.rdf
	Name: del13C_TIC_err
	Units: per mille
	Description: &lt;p&gt;Error of del13C_TIC&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/663910.rdf
	Name: del18O_TIC
	Units: per mille
	Description: &lt;p&gt;Delta 18O of TIC relative to V-PDB&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/663911.rdf
	Name: del18O_TIC_err
	Units: per mille
	Description: &lt;p&gt;Error of del18O_TIC&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/663912.rdf
	Name: del13C_TOC
	Units: per mille
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                <gco:CharacterString>&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Santa Elena samples:&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
&amp;lt;em&amp;gt;Sample preparation and mineralogy:&amp;amp;nbsp;&amp;lt;/em&amp;gt;Ultramafic basement rocks were sampled throughout the Santa Elena Ophiolite. Carbonate deposits associated with Mg-HCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;&amp;amp;nbsp;and Ca-OH springs in the eastern part of the ophiolite were sampled at various locations within riverbeds. All analytical measurements and sample preparation of the Santa Elena peridotites and carbonates were performed at the Department of Geosciences at Virginia Tech. The carbonate samples were prepared by drilling out individual depositional layers of carbonates to reveal heterogeneities within different layers or crushed with an agate mortar for bulk rock analysis. Carbonate precipitates from the rivers were first dried at 40 degrees&amp;amp;nbsp;C in the oven. Before sample analyses all samples were homogenized by hand with the agate mortar. The mineralogy of the carbonates was then determined by X-ray diffraction on a Rigaku MiniFlex XRD using the powder diffraction analysis package PDXL.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Bulk rock powders were prepared for all the ultramafic rocks. To remove contamination from weathering, the outermost 1-2 cm of the rock samples were cut away. The samples were then cleaned in an ultrasonic bath prior to powdering them with a shatter box using an alumina dish. These bulk rock powders were subsequently analyzed for their carbon and sulfur geochemistry.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Carbon geochemistry:&amp;amp;nbsp;&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
Carbon in these systems is typically detected as inorganic (e.g. carbonate carbon) or organic carbon. Organic carbon has also been referred to as reduced carbon or non-carbonate carbon. Here we refer to it as total organic carbon (TOC) even though the presence of traces of graphite and other reduced carbon species cannot entirely be excluded. Total carbon (TC) and total inorganic carbon (TIC) contents, d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C of TC, d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C of TOC and d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C and d&amp;lt;sup&amp;gt;18&amp;lt;/sup&amp;gt;O of TIC were analyzed on the basement rocks, while only d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C and d&amp;lt;sup&amp;gt;18&amp;lt;/sup&amp;gt;O of TIC were determined for the carbonate deposits and precipitates.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;TC contents, d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C&amp;lt;sub&amp;gt;TC&amp;lt;/sub&amp;gt;&amp;amp;nbsp;and d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C&amp;lt;sub&amp;gt;TOC&amp;lt;/sub&amp;gt;&amp;amp;nbsp;were determined on a Vario ISOTOPE elemental analyzer (EA) coupled to an Isoprime 100 isotope ratio mass spectrometer (IRMS). For analyses of d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C&amp;lt;sub&amp;gt;TOC&amp;lt;/sub&amp;gt;, bulk rock samples were reacted for three days with 3N HCl to remove all acid soluble carbon. They were then rinsed with H&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt;O, dried at 40 degrees&amp;amp;nbsp;C in the oven and homogenized in the agate mortar. TC and TOC isotope values are reported in the standard delta&amp;amp;nbsp;notation relative to the Vienna-Pee Dee Belemnite (V-PDB) standard and calibrated to this scale using international [IAEA-CH-6 (sucrose; d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C = -10.449‰) and IAEA-CH-7 (polyethylene; d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C = -32.151‰)] and commercial standards [Elemental Microanalysis wheat flour; d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C = -27.21‰]. TOC was calculated from the difference between TC and TIC and errors are within 10% of the TOC contents. %TIC was calculated as TIC/TC*100. Reproducibility of d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C&amp;lt;sub&amp;gt;TC&amp;lt;/sub&amp;gt;&amp;amp;nbsp;and d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C&amp;lt;sub&amp;gt;TOC&amp;lt;/sub&amp;gt;&amp;amp;nbsp;is better than 0.1‰.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;The d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C&amp;lt;sub&amp;gt;TIC&amp;lt;/sub&amp;gt;&amp;amp;nbsp;and d&amp;lt;sup&amp;gt;18&amp;lt;/sup&amp;gt;O&amp;lt;sub&amp;gt;TIC&amp;lt;/sub&amp;gt;&amp;amp;nbsp;and TIC contents were analyzed on a MultiFlowGeo headspace sampler attached to an Isoprime 100 IRMS. Samples were prepared in septum vials, flushed with helium and acidified with phosphoric acid. Samples were then reacted for at least 3 hours at room temperature if no magnesite was present (as determined by XRD analysis). Magnesite-bearing samples were reacted for 72 hours at 90 degrees&amp;amp;nbsp;C. Carbon and oxygen isotope values are reported in the standard delta-notation relative to the Vienna-Pee Dee Belemnite (V-PDB) standard and calibrated to this scale using the international standards IAEA-CO-1 (marble; d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C = +2.492‰, d&amp;lt;sup&amp;gt;18&amp;lt;/sup&amp;gt;O = -2.4‰), IAEA-CO-9 (BaCO&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;; d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C = -47.321‰, d&amp;lt;sup&amp;gt;18&amp;lt;/sup&amp;gt;O = -15.6‰) and NBS18 (calcite, d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C = -5.014‰, d&amp;lt;sup&amp;gt;18&amp;lt;/sup&amp;gt;O = -23.2‰). Reproducibility for the analysis of the samples was better than +/-&amp;amp;nbsp;0.07‰ for d&amp;lt;sup&amp;gt;13&amp;lt;/sup&amp;gt;C and better than +/-&amp;amp;nbsp;0.3‰ for d&amp;lt;sup&amp;gt;18&amp;lt;/sup&amp;gt;O. Reproducibility of TIC contents is typically better than 5%.&amp;lt;/p&amp;gt;</gco:CharacterString>
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                <gco:CharacterString>&amp;lt;p&amp;gt;TOC calculated as&amp;amp;nbsp; =&amp;amp;nbsp; TC -&amp;amp;nbsp; TIC&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Detection limits:&amp;lt;br /&amp;gt;
Carbon contents: approximately100 ppm&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;BCO-DMO Processing:&amp;lt;br /&amp;gt;
- moved group/sample type into its own column;&amp;lt;br /&amp;gt;
- modified parameter names to conform with BCO-DMO naming conventions;&amp;lt;br /&amp;gt;
- replaced spaces with underscores;&amp;lt;br /&amp;gt;
- repalced &amp;quot;n.d.&amp;quot; with nd, &amp;quot;n.a.&amp;quot; with NA, and &amp;quot;&amp;amp;lt;l.o.d.&amp;quot; with BDL.&amp;lt;/p&amp;gt;</gco:CharacterString>
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