<div><p>In August 2012, September and October 2013, August 2014, and August 2015, 7-40°C diffuse hydrothermal fluids were collected from 10 vent sites at 1515-1716 m depths from Axial Seamount on the Juan de Fuca Ridge. The fluid samples were drawn into 650 ml Tedlar plastic bags with polyethylene valves within rigid housings using the NOAA Hydrothermal Fluid and Particle Sampler. The sampler pumped vent fluid through a titanium nozzle and recorded the temperature of the fluid within the intake nozzle once every second during pumping. Samples were collected using the research submarines Jason II and ROPOS. Background seawater was collected by shipboard hydrocasts at 1500 m depth directly over the caldera (25 m above the bottom) and 3 km west of the summit with 10 L Niskin bottles. The hydrothermal fluid and background seawater samples were divided for cultivation-dependent Most Probable Number (MPN) concentration estimates of thermophiles and hyperthermophiles (100 ml), microcosm incubations (400 ml), and total cell counts (40 ml). All operations at sea occurred on the research vessels Marcus G. Langseth, Thomas G. Thompson, Falkor, and Ronald H. Brown.</p>
<p><strong>Most Probable Number (MPN) Cell Estimates:</strong> Three-tube MPN analyses were used by adding 3.3, 0.33, and 0.03 ml of the hydrothermal fluid samples in triplicate to the methanogen, autotrophic sulfur reducer, and heterotroph media. After inoculation, the tubes were incubated at 80°C and 55°C for up to 7 days. Growth in the tubes was confirmed using phase-contrast light microscopy. Growth of methanogens and H2-producing heterotrophs was verified by analyzing all of the tubes for CH4 and H2, respectively, in the headspace using gas chromatography. Total and H2-producing heterotroph cell concentration estimates were scored and reported separately based on tubes that had cells versus those with H2. In order to estimate the concentration of non-methanogenic autotrophs in the autotrophic sulfur medium, the estimated number of methanogens in the autotrophic sulfur medium MPN tubes was subtracted from the estimated concentration of total cells.</p>
<p><a href="http://dmoserv3.bco-dmo.org/data_docs/NeMO2015/NeMO2015_methods.pdf" target="_blank">Full methodology</a></p></div>
Abundances of various thermophiles, and total CH4 produced in microcosms
<div><p>This dataset includes Most-Probable Number (MPN, L-1) estimates of heterotrophs, H2-producing heterotrophs, methanogens, and non-methanogenic hydrogenotrophs that grow at 55 C and 80 C.</p>
<p><strong>Relevant Reference:</strong> Topcuoglu, BD, Stewart LC, Morrison HG, Butterfield DA, Huber JA and Holden JF (2016) Hydrogen Limitation and Syntrophic Growth among Natural Assemblages of Thermophilic Methanogens at Deep-sea Hydrothermal Vents. Front. Microbiol. 7:1240. doi: <a href="http://journal.frontiersin.org/article/10.3389/fmicb.2016.01240/full" target="_blank">10.3389/fmicb.2016.01240</a></p>
<p><strong>Related Datasets:</strong><br /><a href="http://www.bco-dmo.org/dataset/664201">Axial Seamount hydrothermal sampling sites</a><br /><a href="http://www.bco-dmo.org/dataset/664306">Thermophile growth</a></p></div>
Thermophile abundance - MPN
<div><p><strong>BCO-DMO Processing notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- replaced 'ND' with 'not_detected'<br />
- replaced '-' with nd (no data)<br />
- replaced blank cells with NA (not applicable)<br />
- removed special characters '(' and ')' from site names<br />
- reformatted table into a flat file<br />
- added latitudes and longitudes</p></div>
664280
Thermophile abundance - MPN
2016-11-08T14:03:03-05:00
2016-11-08T14:03:03-05:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:664280
Most probable number of various thermophiles, and total CH4 produced in microcosms from hydrothermal sampling sites from 5 cruises to the Axial Seamount, Juan de Fuca Ridge from 2012 to 2015 (NeMO2015 project)
false
Holden, J., Chadwick, W., Butterfield, D. A. (2016) Most probable number of various thermophiles, and total CH4 produced in microcosms from hydrothermal sampling sites from 5 cruises to the Axial Seamount, Juan de Fuca Ridge from 2012 to 2015 (NeMO2015 project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2016-11-08 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/664280 [access date]
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false
2016-11-08
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