http://lod.bco-dmo.org/id/dataset/664351
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2016-11-10
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Grazing experiment 4: Carbon and Nitrogen data for low-high pCO2 acclimated Rhodomonas sp. cultures (E Hux Response to pCO2 project)
2016-11-10
publication
2016-11-10
revision
BCO-DMO Linked Data URI
2016-11-10
creation
http://lod.bco-dmo.org/id/dataset/664351
M. Brady Olson
Western Washington University
principalInvestigator
Brooke Love
Western Washington University
principalInvestigator
Suzanne Strom
Western Washington University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Olson, M. Brady, Love, B., Strom, S. (2016) Grazing experiment 4: Carbon and Nitrogen data for low-high pCO2 acclimated Rhodomonas sp. cultures (E Hux Response to pCO2 project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2016-11-10 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/664351 [access date]
Carbon and Nitrogen data for low-high pCO2 acclimated Rhodomonas sp. cultures Dataset Description: <p><strong>Related Reference:&nbsp;</strong><br />
Still, Kelly Ann, Microzooplankton grazing, growth and gross growth efficiency are affected by pCO<sub>2</sub> induced changes in phytoplankton biology. (Masters Thesis) Western Washington University.&nbsp;<a href="http://cedar.wwu.edu/cgi/viewcontent.cgi?article=1490&amp;context=wwuet">http://cedar.wwu.edu/cgi/viewcontent.cgi?article=1490&amp;context=wwuet</a></p> Methods and Sampling: <p>The phytoplankton Rhodomonas sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv). Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients. Some of the culture removed was used to evaluate chemical parameters. Samples for particulate cellular carbon and nitrogen were taken by gently vacuum filtering 100 ml from each pCO2 treatment replicate onto 21 mm muffled glass fiber filters.&nbsp; After filtration, filters were removed and placed in tin boats. Samples and controls (media blanks, filter blanks, and capsule blanks) were placed in a drying oven for 24 hours at 60 C, after which time they were removed and placed in a desiccator until analysis. Tin boats containing the filters and controls were folded into pellets and then combusted using a Micro Cube elemental analyzer interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer at the UC Davis Stable Isotope Facility.</p>
<p>This work took place at the Shannon Point Marine Center, Bellingham, Washington.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0961229 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0961229
completed
M. Brady Olson
Western Washington University
(360) 650-7400
1900 Shannon Point Rd.
Anacortes
WA
98221
United States
Brady.Olson@wwu.edu
pointOfContact
Brooke Love
Western Washington University
(360) 650-2894
516 High St. Mail Stop 9181
Bellingham
WA
98225
United States
brooke.love@wwu.edu
pointOfContact
Suzanne Strom
Western Washington University
360-293-2188
Shannon Point Marine Center 1900 Shannon Point Rd
Anacortes
WA
98221
USA
suzanne.strom@wwu.edu
pointOfContact
asNeeded
Unknown
treatment_replicate
C_ug
C_adjusted
N_ug
N_adjusted
cell_count
ml_filtered
total_cells
C_ug_cell
N_ug_cell
C_pg_cell
N_pg_cell
C_to_N
MICRO cube elemental analyzer
PDZ Europa 20-20 isotope ratio mass spectrometer
theme
None, User defined
sample identification
total organic Carbon
total organic Nitrogen
cell_concentration
volume of water filtered
Carbon to Nitrogen ratio (C:N)
featureType
BCO-DMO Standard Parameters
Elemental Analyzer
Isotope-ratio Mass Spectrometer
instrument
BCO-DMO Standard Instruments
Lab_Olson_B
service
Deployment Activity
Bellingham, WA
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton
https://osprey.bco-dmo.org/project/517694
Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton
<p><em><strong>Description from NSF award abstract:</strong></em><br />
The calcifying Haptophyte <em>Emiliania huxleyi </em>appears to be acutely sensitive to the rising concentration of ocean pCO2. Documented responses by <em>E. huxleyi </em>to elevated pCO2 include modifications to their calcification rate and cell size, malformation of coccoliths, elevated growth rates, increased organic carbon production, lowering of PIC:POC ratios, and elevated production of the active climate gas DMS. Changes in these parameters are mechanisms known to elicit alterations in grazing behavior by microzooplankton, the oceans dominant grazer functional group. The investigators hypothesize that modifications to the physiology and biochemistry of calcifying and non-calcifying Haptophyte <em>Emiliania huxleyi </em>in response to elevated pCO2 will precipitate alterations in microzooplankton grazing dynamics. To test this hypothesis, they will conduct controlled laboratory experiments where several strains of <em>E. huxleyi</em> are grown at several CO2 concentrations. After careful characterization of the biochemical and physiological responses of the <em>E. huxleyi</em> strains to elevated pCO2, they will provide these strains as food to several ecologically-important microzooplankton and document grazing dynamics.<em> E. huxleyi</em> is an ideal organism for the study of phytoplankton and microzooplankton responses to rising anthropogenic CO2, the effects of which in the marine environment are called ocean acidification; <em>E. huxleyi </em>is biogeochemically important, is well studied, numerous strains are in culture that exhibit variation in the parameters described above, and they are readily fed upon by ecologically important microzooplankton.</p>
<p>The implications of changes in microzooplankton grazing for carbon cycling, specifically CaCO3 export, DMS production, nutrient regeneration in surface waters, and carbon transfer between trophic levels are profound, as this grazing, to a large degree, regulates all these processes. <em>E. huxleyi </em>is a model prey organism because it is one of the most biogeochemically influential global phytoplankton. It forms massive seasonal blooms, contributes significantly to marine inorganic and organic carbon cycles, is a large producer of the climatically active gas DMS, and is a source of organic matter for trophic levels both above and below itself. The planned controlled study will increase our knowledge of the mechanisms that drive patterns of change between trophic levels, thus providing a wider array of tools necessary to understand the complex nature of ocean acidification field studies, where competing variables can confound precise interpretation.</p>
E Hux Response to pCO2
largerWorkCitation
project
eng; USA
biota
oceans
Bellingham, WA
2016-11-10
0
BCO-DMO catalogue of parameters from Grazing experiment 4: Carbon and Nitrogen data for low-high pCO2 acclimated Rhodomonas sp. cultures (E Hux Response to pCO2 project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/664409.rdf
Name: treatment_replicate
Units: unitless
Description: <p>sample identifier: treatment replicate that names the sample and the day of semi-continuous culture or blank type</p>
http://lod.bco-dmo.org/id/dataset-parameter/664410.rdf
Name: C_ug
Units: micrograms (ug)
Description: <p>carbon in sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/664411.rdf
Name: C_adjusted
Units: micrograms (ug)
Description: <p>carbon minus the media blank</p>
http://lod.bco-dmo.org/id/dataset-parameter/664412.rdf
Name: N_ug
Units: micrograms (ug)
Description: <p>nitrogen in sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/664413.rdf
Name: N_adjusted
Units: micrograms (ug)
Description: <p>nitrogen minus the media blank</p>
http://lod.bco-dmo.org/id/dataset-parameter/664414.rdf
Name: cell_count
Units: cells/milliliter
Description: <p>cells per ml in the daily sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/664415.rdf
Name: ml_filtered
Units: milliliters
Description: <p>volume of sample filtered</p>
http://lod.bco-dmo.org/id/dataset-parameter/664416.rdf
Name: total_cells
Units: cells
Description: <p>total number of cell on the filter</p>
http://lod.bco-dmo.org/id/dataset-parameter/664417.rdf
Name: C_ug_cell
Units: micrograms/cell (ug/cell)
Description: <p>carbon per cell</p>
http://lod.bco-dmo.org/id/dataset-parameter/664418.rdf
Name: N_ug_cell
Units: micrograms/cell (ug/cell)
Description: <p>nitrogen per cell</p>
http://lod.bco-dmo.org/id/dataset-parameter/664419.rdf
Name: C_pg_cell
Units: picograms/cell (pg/cell)
Description: <p>carbon per cell</p>
http://lod.bco-dmo.org/id/dataset-parameter/664420.rdf
Name: N_pg_cell
Units: picograms/cell (pg/cell)
Description: <p>nitrogen per cell</p>
http://lod.bco-dmo.org/id/dataset-parameter/664421.rdf
Name: C_to_N
Units: dimensionless
Description: <p>ratio of carbon to nitrogen</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2058
https://datadocs.bco-dmo.org/file/nGGl055sXPNALE/expt4_CN.csv
expt4_CN.csv
Primary data file for dataset ID 664351
download
https://osprey.bco-dmo.org/dataset/664351/data/download
download
onLine
dataset
<p>The phytoplankton Rhodomonas sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv). Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients. Some of the culture removed was used to evaluate chemical parameters. Samples for particulate cellular carbon and nitrogen were taken by gently vacuum filtering 100 ml from each pCO2 treatment replicate onto 21 mm muffled glass fiber filters.&nbsp; After filtration, filters were removed and placed in tin boats. Samples and controls (media blanks, filter blanks, and capsule blanks) were placed in a drying oven for 24 hours at 60 C, after which time they were removed and placed in a desiccator until analysis. Tin boats containing the filters and controls were folded into pellets and then combusted using a Micro Cube elemental analyzer interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer at the UC Davis Stable Isotope Facility.</p>
<p>This work took place at the Shannon Point Marine Center, Bellingham, Washington.</p>
Specified by the Principal Investigator(s)
<p>Picograms per cell for carbon and nitrogen were calculated based on standard curves and were then normalized to per cell based on cell counts for the sample day.</p>
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- nd (no data) was entered into all blank cells</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
MICRO cube elemental analyzer
MICRO cube elemental analyzer
PI Supplied Instrument Name: MICRO cube elemental analyzer PI Supplied Instrument Description:Measured carbon and nitrogen composition: Micro Cube elemental analyzer interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer at the UC Davis Stable Isotope Facility. Instrument Name: Elemental Analyzer Instrument Short Name: Instrument Description: Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/
PDZ Europa 20-20 isotope ratio mass spectrometer
PDZ Europa 20-20 isotope ratio mass spectrometer
PI Supplied Instrument Name: PDZ Europa 20-20 isotope ratio mass spectrometer PI Supplied Instrument Description:Measured carbon and nitrogen composition: Micro Cube elemental analyzer interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer at the UC Davis Stable Isotope Facility. Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Deployment: Lab_Olson_B
Lab_Olson_B
WWU
laboratory
WWU
laboratory