http://lod.bco-dmo.org/id/dataset/668747
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2016-12-06
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Short term data for low-high pCO2 acclimated Rhodomonas sp. cultures ingested by Favella grazers (E Hux Response to pCO2 project)
2016-12-06
publication
2016-12-06
revision
BCO-DMO Linked Data URI
2016-12-06
creation
http://lod.bco-dmo.org/id/dataset/668747
Brady M. Olson
Western Washington University
principalInvestigator
Dr Brooke Love
Western Washington University
principalInvestigator
Suzanne Strom
Western Washington University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Olson, B. M., Love, B., Strom, S. (2016) Short term data for low-high pCO2 acclimated Rhodomonas sp. cultures ingested by Favella grazers (E Hux Response to pCO2 project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2016-12-06 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/668747 [access date]
Short term data for low-high pCO2 acclimated Rhodomonas sp. cultures ingested by Favella grazers Dataset Description: <p><strong>Related Reference:&nbsp;</strong></p>
<p>Still, Kelly Ann, Microzooplankton grazing, growth and gross growth efficiency are affected by pCO<sub>2</sub> induced changes in phytoplankton biology. (Masters Thesis) Western Washington University.&nbsp;<a href="http://cedar.wwu.edu/cgi/viewcontent.cgi?article=1490&amp;context=wwuet">http://cedar.wwu.edu/cgi/viewcontent.cgi?article=1490&amp;context=wwuet</a></p> Methods and Sampling: <p>The phytoplankton <em>Rhodomonas </em>sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv).&nbsp; Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients.&nbsp; On day 9 <em>Rhodomonas </em>cells from the treatments were fed to starved <em>Favella </em><em>ehrenbergii</em> cells for 15, 30, and 45 minutes.&nbsp; A 24-hour time point was also taken but scored at either “1” for containing any food or “0” for no food in the vacuole. During each sampling time point, 20 ml&nbsp;of experiment volume was removed, fixed with 0.5% glutaraldehyde and stained with DAPI.&nbsp; Samples were stored at 4° C for 12 hours to allow time for the DAPI to stain.&nbsp; This volume was filtered onto a 20 µm pore size polycarbonate filter with, which was then put on a slide with immersion oil and kept frozen until evaluation.&nbsp; Slides were evaluated under 400x using an epi-fluorescent microscope under blue-light excitation.&nbsp; The first 100 microzooplankton on each slide were assessed for each replicate/treatment, and individual prey cells were counted in the grazer food vacuole.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0961229 Award URL: http://www.nsf.gov/awardsearch/showAward?AWD_ID=0961229
completed
Brady M. Olson
Western Washington University
(360) 650-7400
1900 Shannon Point Rd.
Anacortes
WA
98221
United States
Brady.Olson@wwu.edu
pointOfContact
Dr Brooke Love
Western Washington University
(360) 650-2894
516 High St. Mail Stop 9181
Bellingham
WA
98225
United States
brooke.love@wwu.edu
pointOfContact
Suzanne Strom
Western Washington University
360-293-2188
Shannon Point Marine Center 1900 Shannon Point Rd
Anacortes
WA
98221
USA
suzanne.strom@wwu.edu
pointOfContact
asNeeded
Unknown
Grazer_Analyzed
amb_15min_A
amb_15min_B
amb_15min_C
amb_45min_A
amb_45min_B
amb_45min_C
amb_90min_A
amb_90min_B
amb_90min_C
amb_24hr_A
amb_24hr_B
amb_24hr_C
mod_15min_A
mod_15min_B
mod_15min_C
mod_45min_A
mod_45min_B
mod_45min_C
mod_90min_A
mod_90min_B
mod_90min_C
mod_24hr_A
mod_24hr_B
mod_24hr_C
high_15min_A
high_15min_B
high_15min_C
high_45min_A
high_45min_B
high_45min_C
high_90min_A
high_90min_B
high_90min_C
high_24hr_A
high_24hr_B
high_24hr_C
epi-fluorescent microscope under blue-light excitation
theme
None, User defined
sample identification
count
featureType
BCO-DMO Standard Parameters
Fluorescence Microscope
instrument
BCO-DMO Standard Instruments
Lab_Olson_B
service
Deployment Activity
Bellingham, WA
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton
https://www.bco-dmo.org/project/517694
Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton
<p><em><strong>Description from NSF award abstract:</strong></em><br />
The calcifying Haptophyte <em>Emiliania huxleyi </em>appears to be acutely sensitive to the rising concentration of ocean pCO2. Documented responses by <em>E. huxleyi </em>to elevated pCO2 include modifications to their calcification rate and cell size, malformation of coccoliths, elevated growth rates, increased organic carbon production, lowering of PIC:POC ratios, and elevated production of the active climate gas DMS. Changes in these parameters are mechanisms known to elicit alterations in grazing behavior by microzooplankton, the oceans dominant grazer functional group. The investigators hypothesize that modifications to the physiology and biochemistry of calcifying and non-calcifying Haptophyte <em>Emiliania huxleyi </em>in response to elevated pCO2 will precipitate alterations in microzooplankton grazing dynamics. To test this hypothesis, they will conduct controlled laboratory experiments where several strains of <em>E. huxleyi</em> are grown at several CO2 concentrations. After careful characterization of the biochemical and physiological responses of the <em>E. huxleyi</em> strains to elevated pCO2, they will provide these strains as food to several ecologically-important microzooplankton and document grazing dynamics.<em> E. huxleyi</em> is an ideal organism for the study of phytoplankton and microzooplankton responses to rising anthropogenic CO2, the effects of which in the marine environment are called ocean acidification; <em>E. huxleyi </em>is biogeochemically important, is well studied, numerous strains are in culture that exhibit variation in the parameters described above, and they are readily fed upon by ecologically important microzooplankton.</p>
<p>The implications of changes in microzooplankton grazing for carbon cycling, specifically CaCO3 export, DMS production, nutrient regeneration in surface waters, and carbon transfer between trophic levels are profound, as this grazing, to a large degree, regulates all these processes. <em>E. huxleyi </em>is a model prey organism because it is one of the most biogeochemically influential global phytoplankton. It forms massive seasonal blooms, contributes significantly to marine inorganic and organic carbon cycles, is a large producer of the climatically active gas DMS, and is a source of organic matter for trophic levels both above and below itself. The planned controlled study will increase our knowledge of the mechanisms that drive patterns of change between trophic levels, thus providing a wider array of tools necessary to understand the complex nature of ocean acidification field studies, where competing variables can confound precise interpretation.</p>
E Hux Response to pCO2
largerWorkCitation
project
eng; USA
biota
oceans
Bellingham, WA
2016-12-06
0
BCO-DMO catalogue of parameters from Short term data for low-high pCO2 acclimated Rhodomonas sp. cultures ingested by Favella grazers (E Hux Response to pCO2 project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/668757.rdf
Name: Grazer_Analyzed
Units: unitless
Description: sample identifier: individual grazer analyzed
http://lod.bco-dmo.org/id/dataset-parameter/668758.rdf
Name: amb_15min_A
Units: cells
Description: Rhodomonas cells counted inside the grazer: ambient pCO2 level; 15 minute grazing period; replicate A
http://lod.bco-dmo.org/id/dataset-parameter/668759.rdf
Name: amb_15min_B
Units: cells
Description: Rhodomonas cells counted inside the grazer: ambient pCO2 level; 15 minute grazing period; replicate B
http://lod.bco-dmo.org/id/dataset-parameter/668760.rdf
Name: amb_15min_C
Units: cells
Description: Rhodomonas cells counted inside the grazer: ambient pCO2 level; 15 minute grazing period; replicate C
http://lod.bco-dmo.org/id/dataset-parameter/668761.rdf
Name: amb_45min_A
Units: cells
Description: Rhodomonas cells counted inside the grazer: ambient pCO2 level; 45 minute grazing period; replicate A
http://lod.bco-dmo.org/id/dataset-parameter/668762.rdf
Name: amb_45min_B
Units: cells
Description: Rhodomonas cells counted inside the grazer: ambient pCO2 level; 45 minute grazing period; replicate B
http://lod.bco-dmo.org/id/dataset-parameter/668763.rdf
Name: amb_45min_C
Units: cells
Description: Rhodomonas cells counted inside the grazer: ambient pCO2 level; 15 minute grazing period; replicate C
http://lod.bco-dmo.org/id/dataset-parameter/668764.rdf
Name: amb_90min_A
Units: cells
Description: Rhodomonas cells counted inside the grazer: ambient pCO2 level; 90 minute grazing period; replicate A
http://lod.bco-dmo.org/id/dataset-parameter/668765.rdf
Name: amb_90min_B
Units: cells
Description: Rhodomonas cells counted inside the grazer: ambient pCO2 level; 90 minute grazing period; replicate B
http://lod.bco-dmo.org/id/dataset-parameter/668766.rdf
Name: amb_90min_C
Units: cells
Description: Rhodomonas cells counted inside the grazer: ambient pCO2 level; 90 minute grazing period; replicate C
http://lod.bco-dmo.org/id/dataset-parameter/668767.rdf
Name: amb_24hr_A
Units: cells
Description: Rhodomonas cells counted inside the grazer: ambient pCO2 level; 24 hour grazing period; replicate A
http://lod.bco-dmo.org/id/dataset-parameter/668768.rdf
Name: amb_24hr_B
Units: cells
Description: Rhodomonas cells counted inside the grazer: ambient pCO2 level; 24 hour grazing period; replicate B
http://lod.bco-dmo.org/id/dataset-parameter/668769.rdf
Name: amb_24hr_C
Units: cells
Description: Rhodomonas cells counted inside the grazer: ambient pCO2 level; 24 hour grazing period; replicate C
http://lod.bco-dmo.org/id/dataset-parameter/668770.rdf
Name: mod_15min_A
Units: cells
Description: Rhodomonas cells counted inside the grazer: moderate pCO2 level; 15 minute grazing period; replicate A
http://lod.bco-dmo.org/id/dataset-parameter/668771.rdf
Name: mod_15min_B
Units: cells
Description: Rhodomonas cells counted inside the grazer: moderate pCO2 level; 15 minute grazing period; replicate B
http://lod.bco-dmo.org/id/dataset-parameter/668772.rdf
Name: mod_15min_C
Units: cells
Description: Rhodomonas cells counted inside the grazer: moderate pCO2 level; 15 minute grazing period; replicate C
http://lod.bco-dmo.org/id/dataset-parameter/668773.rdf
Name: mod_45min_A
Units: cells
Description: Rhodomonas cells counted inside the grazer: moderate pCO2 level; 45 minute grazing period; replicate A
http://lod.bco-dmo.org/id/dataset-parameter/668774.rdf
Name: mod_45min_B
Units: cells
Description: Rhodomonas cells counted inside the grazer: moderate pCO2 level; 45 minute grazing period; replicate B
http://lod.bco-dmo.org/id/dataset-parameter/668775.rdf
Name: mod_45min_C
Units: cells
Description: Rhodomonas cells counted inside the grazer: moderate pCO2 level; 15 minute grazing period; replicate C
http://lod.bco-dmo.org/id/dataset-parameter/668776.rdf
Name: mod_90min_A
Units: cells
Description: Rhodomonas cells counted inside the grazer: moderate pCO2 level; 90 minute grazing period; replicate A
http://lod.bco-dmo.org/id/dataset-parameter/668777.rdf
Name: mod_90min_B
Units: cells
Description: Rhodomonas cells counted inside the grazer: moderate pCO2 level; 90 minute grazing period; replicate B
http://lod.bco-dmo.org/id/dataset-parameter/668778.rdf
Name: mod_90min_C
Units: cells
Description: Rhodomonas cells counted inside the grazer: moderate pCO2 level; 90 minute grazing period; replicate C
http://lod.bco-dmo.org/id/dataset-parameter/668779.rdf
Name: mod_24hr_A
Units: cells
Description: Rhodomonas cells counted inside the grazer: moderate pCO2 level; 24 hour grazing period; replicate A
http://lod.bco-dmo.org/id/dataset-parameter/668780.rdf
Name: mod_24hr_B
Units: cells
Description: Rhodomonas cells counted inside the grazer: moderate pCO2 level; 24 hour grazing period; replicate B
http://lod.bco-dmo.org/id/dataset-parameter/668781.rdf
Name: mod_24hr_C
Units: cells
Description: Rhodomonas cells counted inside the grazer: moderate pCO2 level; 24 hour grazing period; replicate C
http://lod.bco-dmo.org/id/dataset-parameter/668782.rdf
Name: high_15min_A
Units: cells
Description: Rhodomonas cells counted inside the grazer: high pCO2 level; 15 minute grazing period; replicate A
http://lod.bco-dmo.org/id/dataset-parameter/668783.rdf
Name: high_15min_B
Units: cells
Description: Rhodomonas cells counted inside the grazer: high pCO2 level; 15 minute grazing period; replicate B
http://lod.bco-dmo.org/id/dataset-parameter/668784.rdf
Name: high_15min_C
Units: cells
Description: Rhodomonas cells counted inside the grazer: high pCO2 level; 15 minute grazing period; replicate C
http://lod.bco-dmo.org/id/dataset-parameter/668785.rdf
Name: high_45min_A
Units: cells
Description: Rhodomonas cells counted inside the grazer: high pCO2 level; 45 minute grazing period; replicate A
http://lod.bco-dmo.org/id/dataset-parameter/668786.rdf
Name: high_45min_B
Units: cells
Description: Rhodomonas cells counted inside the grazer: high pCO2 level; 45 minute grazing period; replicate B
http://lod.bco-dmo.org/id/dataset-parameter/668787.rdf
Name: high_45min_C
Units: cells
Description: Rhodomonas cells counted inside the grazer: high pCO2 level; 15 minute grazing period; replicate C
http://lod.bco-dmo.org/id/dataset-parameter/668788.rdf
Name: high_90min_A
Units: cells
Description: Rhodomonas cells counted inside the grazer: high pCO2 level; 90 minute grazing period; replicate A
http://lod.bco-dmo.org/id/dataset-parameter/668789.rdf
Name: high_90min_B
Units: cells
Description: Rhodomonas cells counted inside the grazer: high pCO2 level; 90 minute grazing period; replicate B
http://lod.bco-dmo.org/id/dataset-parameter/668790.rdf
Name: high_90min_C
Units: cells
Description: Rhodomonas cells counted inside the grazer: high pCO2 level; 90 minute grazing period; replicate C
http://lod.bco-dmo.org/id/dataset-parameter/668791.rdf
Name: high_24hr_A
Units: cells
Description: Rhodomonas cells counted inside the grazer: high pCO2 level; 24 hour grazing period; replicate A
http://lod.bco-dmo.org/id/dataset-parameter/668792.rdf
Name: high_24hr_B
Units: cells
Description: Rhodomonas cells counted inside the grazer: high pCO2 level; 24 hour grazing period; replicate B
http://lod.bco-dmo.org/id/dataset-parameter/668793.rdf
Name: high_24hr_C
Units: cells
Description: Rhodomonas cells counted inside the grazer: high pCO2 level; 24 hour grazing period; replicate C
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
10659
https://datadocs.bco-dmo.org/file/R88Xm6kSvy602M/expt4b_Fav_grazing.csv
expt4b_Fav_grazing.csv
Primary data file for dataset ID 668747
download
https://www.bco-dmo.org/dataset/668747/data/download
download
onLine
dataset
<p>The phytoplankton <em>Rhodomonas </em>sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv).&nbsp; Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients.&nbsp; On day 9 <em>Rhodomonas </em>cells from the treatments were fed to starved <em>Favella </em><em>ehrenbergii</em> cells for 15, 30, and 45 minutes.&nbsp; A 24-hour time point was also taken but scored at either “1” for containing any food or “0” for no food in the vacuole. During each sampling time point, 20 ml&nbsp;of experiment volume was removed, fixed with 0.5% glutaraldehyde and stained with DAPI.&nbsp; Samples were stored at 4° C for 12 hours to allow time for the DAPI to stain.&nbsp; This volume was filtered onto a 20 µm pore size polycarbonate filter with, which was then put on a slide with immersion oil and kept frozen until evaluation.&nbsp; Slides were evaluated under 400x using an epi-fluorescent microscope under blue-light excitation.&nbsp; The first 100 microzooplankton on each slide were assessed for each replicate/treatment, and individual prey cells were counted in the grazer food vacuole.</p>
Specified by the Principal Investigator(s)
<p>These data are unprocessed counts of the Rhodomonas cells ingested by each Favella grazer.</p>
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- nd (no data) was entered into all blank cells</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
epi-fluorescent microscope under blue-light excitation
epi-fluorescent microscope under blue-light excitation
PI Supplied Instrument Name: epi-fluorescent microscope under blue-light excitation PI Supplied Instrument Description:For cell counts Instrument Name: Fluorescence Microscope Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB06/
Deployment: Lab_Olson_B
Lab_Olson_B
WWU
laboratory
WWU
laboratory