|Olson, Brady M.||Western Washington University (WWU)||Principal Investigator|
|Love, Brooke||Western Washington University (WWU)||Co-Principal Investigator|
|Strom, Suzanne||Western Washington University (WWU)||Co-Principal Investigator|
|Still, Kelly Ann||Western Washington University (WWU)||Student|
|Copley, Nancy||Woods Hole Oceanographic Institution (WHOI BCO-DMO)||BCO-DMO Data Manager|
Still, Kelly Ann, Microzooplankton grazing, growth and gross growth efficiency are affected by pCO2 induced changes in phytoplankton biology. (Masters Thesis) Western Washington University. http://cedar.wwu.edu/cgi/viewcontent.cgi?article=1490&context=wwuet
The phytoplankton Rhodomonas sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv). Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients. Some of the culture removed was used to evaluate chemical parameters. For Chlorophyll-a analysis 10 ml of each culture replicate was filtered onto a glass fiber filter. Filters were immediately folded and placed in test tubes containing 6 ml of 90% v/v acetone and stored at -20°C for 24 hours. Samples were then warmed to room temperature in the dark, filters were removed and tubes were centrifuged before being analyzed on a Turner Designs Trilogy fluorometer. Raw fluorescence pre- and post-addition of 10% HCL was used to calculate Chl a.
Chl-a (µg/ml) = (K*Fm*ext.vol (ml)*(Fo-Fa))/ (L filtered-1)
These are unprocessed Chl data.
BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
- replaced spaces with underscores
|sample_day_treatment_rep||sample identifier: treatment replicate that names the sample and the day of semi-continuous culture||unitless|
|inst_k||the instrument sensitivity coefficient||unitless|
|Fo||the raw fluorescence reading of the extract||unitless|
|Fa||the raw fluorescence reading of the acidified extract||unitless|
|Fm||fluorescence maximum obtained using pure Chl-a standard obtained by dividing Fo by Fa||unitless|
|vol_extract||the amount of 90% acetone the filter was extracted in||milliliters|
|dilution_factor||dilution factor is used if the extract is diluted||unitless|
|chla_ug_L||Chlorophyll-a concentration||micrograms/liter (ug/L)|
|cell_concentration||cell concentration on sample day||cells/milliliter|
|chla_pg_cell||cell concentration on sample day||picograms/milliliter (pg/ml)|
|Dataset-specific Instrument Name|| |
Turner Designs Trilogy fluorometer
|Generic Instrument Name|| |
|Generic Instrument Description|| |
A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.
|Start Date|| |
|End Date|| |
Description from NSF award abstract:
The calcifying Haptophyte Emiliania huxleyi appears to be acutely sensitive to the rising concentration of ocean pCO2. Documented responses by E. huxleyi to elevated pCO2 include modifications to their calcification rate and cell size, malformation of coccoliths, elevated growth rates, increased organic carbon production, lowering of PIC:POC ratios, and elevated production of the active climate gas DMS. Changes in these parameters are mechanisms known to elicit alterations in grazing behavior by microzooplankton, the oceans dominant grazer functional group. The investigators hypothesize that modifications to the physiology and biochemistry of calcifying and non-calcifying Haptophyte Emiliania huxleyi in response to elevated pCO2 will precipitate alterations in microzooplankton grazing dynamics. To test this hypothesis, they will conduct controlled laboratory experiments where several strains of E. huxleyi are grown at several CO2 concentrations. After careful characterization of the biochemical and physiological responses of the E. huxleyi strains to elevated pCO2, they will provide these strains as food to several ecologically-important microzooplankton and document grazing dynamics. E. huxleyi is an ideal organism for the study of phytoplankton and microzooplankton responses to rising anthropogenic CO2, the effects of which in the marine environment are called ocean acidification; E. huxleyi is biogeochemically important, is well studied, numerous strains are in culture that exhibit variation in the parameters described above, and they are readily fed upon by ecologically important microzooplankton.
The implications of changes in microzooplankton grazing for carbon cycling, specifically CaCO3 export, DMS production, nutrient regeneration in surface waters, and carbon transfer between trophic levels are profound, as this grazing, to a large degree, regulates all these processes. E. huxleyi is a model prey organism because it is one of the most biogeochemically influential global phytoplankton. It forms massive seasonal blooms, contributes significantly to marine inorganic and organic carbon cycles, is a large producer of the climatically active gas DMS, and is a source of organic matter for trophic levels both above and below itself. The planned controlled study will increase our knowledge of the mechanisms that drive patterns of change between trophic levels, thus providing a wider array of tools necessary to understand the complex nature of ocean acidification field studies, where competing variables can confound precise interpretation.