http://lod.bco-dmo.org/id/dataset/669424
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2016-12-09
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Grazing experiment 5: Chlorophyll-a data for low-high pCO2 acclimated Rhodomonas sp. cultures from 2011-2016 (E Hux Response to pCO2 project)
2016-12-09
publication
2016-12-09
revision
BCO-DMO Linked Data URI
2016-12-09
creation
http://lod.bco-dmo.org/id/dataset/669424
M. Brady Olson
Western Washington University
principalInvestigator
Brooke Love
Western Washington University
principalInvestigator
Suzanne Strom
Western Washington University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Olson, M. Brady, Love, B., Strom, S. (2016) Grazing experiment 5: Chlorophyll-a data for low-high pCO2 acclimated Rhodomonas sp. cultures from 2011-2016 (E Hux Response to pCO2 project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2016-12-09 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/669424 [access date]
Chlorophyll a data for low-high pCO2 acclimated Rhodomonas sp. cultures Dataset Description: <p><strong>Related Reference:&nbsp;</strong><br />
Still, Kelly Ann, Microzooplankton grazing, growth and gross growth efficiency are affected by pCO<sub>2</sub> induced changes in phytoplankton biology. (Masters Thesis) Western Washington University.&nbsp;<a href="http://cedar.wwu.edu/cgi/viewcontent.cgi?article=1490&amp;context=wwuet">http://cedar.wwu.edu/cgi/viewcontent.cgi?article=1490&amp;context=wwuet</a></p> Methods and Sampling: <p>The phytoplankton Rhodomonas sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv).&nbsp; Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients.&nbsp; Some of the culture removed was used to evaluate chemical parameters.&nbsp; For Chlorophyll-a analysis 10 ml of each culture replicate was filtered onto a glass fiber filter.&nbsp; Filters were immediately folded and placed in test tubes containing 6 ml of 90% v/v acetone and stored at&nbsp; &nbsp;-20°C for 24 hours.&nbsp; Samples were then warmed to room temperature in the dark, filters were removed and tubes were centrifuged before being analyzed on a Turner Designs Trilogy fluorometer. Raw fluorescence pre- and post-addition of 10% HCL was used to calculate Chl a.</p>
<p>Chl-a (µg/ml) = (K*Fm*ext.vol (ml)*(Fo-Fa))/ (L filtered-1)</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0961229 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0961229
completed
M. Brady Olson
Western Washington University
(360) 650-7400
1900 Shannon Point Rd.
Anacortes
WA
98221
United States
Brady.Olson@wwu.edu
pointOfContact
Brooke Love
Western Washington University
(360) 650-2894
516 High St. Mail Stop 9181
Bellingham
WA
98225
United States
brooke.love@wwu.edu
pointOfContact
Suzanne Strom
Western Washington University
360-293-2188
Shannon Point Marine Center 1900 Shannon Point Rd
Anacortes
WA
98221
USA
suzanne.strom@wwu.edu
pointOfContact
asNeeded
Unknown
sample_day_treatment_rep
inst_k
Fo
Fa
Fm
filt_vol
vol_extract
dilution_factor
chla_ug_L
cell_concentration
chla_pg_cell
Turner Designs Trilogy fluorometer
theme
None, User defined
sample identification
No BCO-DMO term
volume of water filtered
volume
chlorophyll a
cell_concentration
featureType
BCO-DMO Standard Parameters
Fluorometer
instrument
BCO-DMO Standard Instruments
Lab_Olson_B
service
Deployment Activity
Bellingham, WA
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton
https://osprey.bco-dmo.org/project/517694
Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton
<p><em><strong>Description from NSF award abstract:</strong></em><br />
The calcifying Haptophyte <em>Emiliania huxleyi </em>appears to be acutely sensitive to the rising concentration of ocean pCO2. Documented responses by <em>E. huxleyi </em>to elevated pCO2 include modifications to their calcification rate and cell size, malformation of coccoliths, elevated growth rates, increased organic carbon production, lowering of PIC:POC ratios, and elevated production of the active climate gas DMS. Changes in these parameters are mechanisms known to elicit alterations in grazing behavior by microzooplankton, the oceans dominant grazer functional group. The investigators hypothesize that modifications to the physiology and biochemistry of calcifying and non-calcifying Haptophyte <em>Emiliania huxleyi </em>in response to elevated pCO2 will precipitate alterations in microzooplankton grazing dynamics. To test this hypothesis, they will conduct controlled laboratory experiments where several strains of <em>E. huxleyi</em> are grown at several CO2 concentrations. After careful characterization of the biochemical and physiological responses of the <em>E. huxleyi</em> strains to elevated pCO2, they will provide these strains as food to several ecologically-important microzooplankton and document grazing dynamics.<em> E. huxleyi</em> is an ideal organism for the study of phytoplankton and microzooplankton responses to rising anthropogenic CO2, the effects of which in the marine environment are called ocean acidification; <em>E. huxleyi </em>is biogeochemically important, is well studied, numerous strains are in culture that exhibit variation in the parameters described above, and they are readily fed upon by ecologically important microzooplankton.</p>
<p>The implications of changes in microzooplankton grazing for carbon cycling, specifically CaCO3 export, DMS production, nutrient regeneration in surface waters, and carbon transfer between trophic levels are profound, as this grazing, to a large degree, regulates all these processes. <em>E. huxleyi </em>is a model prey organism because it is one of the most biogeochemically influential global phytoplankton. It forms massive seasonal blooms, contributes significantly to marine inorganic and organic carbon cycles, is a large producer of the climatically active gas DMS, and is a source of organic matter for trophic levels both above and below itself. The planned controlled study will increase our knowledge of the mechanisms that drive patterns of change between trophic levels, thus providing a wider array of tools necessary to understand the complex nature of ocean acidification field studies, where competing variables can confound precise interpretation.</p>
E Hux Response to pCO2
largerWorkCitation
project
eng; USA
biota
oceans
Bellingham, WA
2016-12-09
0
BCO-DMO catalogue of parameters from Grazing experiment 5: Chlorophyll-a data for low-high pCO2 acclimated Rhodomonas sp. cultures from 2011-2016 (E Hux Response to pCO2 project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/669436.rdf
Name: sample_day_treatment_rep
Units: unitless
Description: <p>sample identifier: treatment replicate that names the sample and the day of semi-continuous culture</p>
http://lod.bco-dmo.org/id/dataset-parameter/669437.rdf
Name: inst_k
Units: unitless
Description: <p>the instrument sensitivity coefficient</p>
http://lod.bco-dmo.org/id/dataset-parameter/669438.rdf
Name: Fo
Units: unitless
Description: <p>the raw fluorescence reading of the extract</p>
http://lod.bco-dmo.org/id/dataset-parameter/669439.rdf
Name: Fa
Units: unitless
Description: <p>the raw fluorescence reading of the acidified extract</p>
http://lod.bco-dmo.org/id/dataset-parameter/669440.rdf
Name: Fm
Units: unitless
Description: <p>fluorescence maximum obtained using pure Chl-a standard obtained by dividing Fo by Fa</p>
http://lod.bco-dmo.org/id/dataset-parameter/669441.rdf
Name: filt_vol
Units: liters
Description: <p>filtered volume</p>
http://lod.bco-dmo.org/id/dataset-parameter/669442.rdf
Name: vol_extract
Units: milliliters
Description: <p>the amount of 90% acetone the filter was extracted in</p>
http://lod.bco-dmo.org/id/dataset-parameter/669443.rdf
Name: dilution_factor
Units: unitless
Description: <p>dilution factor is used if the extract is diluted</p>
http://lod.bco-dmo.org/id/dataset-parameter/669444.rdf
Name: chla_ug_L
Units: micrograms/liter (ug/L)
Description: <p>Chlorophyll-a concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/669445.rdf
Name: cell_concentration
Units: cells/milliliter
Description: <p>cell concentration on sample day</p>
http://lod.bco-dmo.org/id/dataset-parameter/669446.rdf
Name: chla_pg_cell
Units: picograms/milliliter (pg/ml)
Description: <p>cell concentration on sample day</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
1291
https://datadocs.bco-dmo.org/file/N77GgkWijwMpZ5/expt5_Chla.csv
expt5_Chla.csv
Primary data file for dataset ID 669424
download
https://osprey.bco-dmo.org/dataset/669424/data/download
download
onLine
dataset
<p>The phytoplankton Rhodomonas sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv).&nbsp; Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients.&nbsp; Some of the culture removed was used to evaluate chemical parameters.&nbsp; For Chlorophyll-a analysis 10 ml of each culture replicate was filtered onto a glass fiber filter.&nbsp; Filters were immediately folded and placed in test tubes containing 6 ml of 90% v/v acetone and stored at&nbsp; &nbsp;-20°C for 24 hours.&nbsp; Samples were then warmed to room temperature in the dark, filters were removed and tubes were centrifuged before being analyzed on a Turner Designs Trilogy fluorometer. Raw fluorescence pre- and post-addition of 10% HCL was used to calculate Chl a.</p>
<p>Chl-a (µg/ml) = (K*Fm*ext.vol (ml)*(Fo-Fa))/ (L filtered-1)</p>
Specified by the Principal Investigator(s)
<p>These are unprocessed Chl data.&nbsp;</p>
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- replaced spaces with underscores</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Turner Designs Trilogy fluorometer
Turner Designs Trilogy fluorometer
PI Supplied Instrument Name: Turner Designs Trilogy fluorometer Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/
Deployment: Lab_Olson_B
Lab_Olson_B
WWU
laboratory
WWU
laboratory