Grazing experiment 5: Carbonate chemistry of Rhodomonas for low-high pCO2 long term experiment treatments, 2011-2016 (E Hux Response to pCO2 project)

Website: https://www.bco-dmo.org/dataset/669472
Data Type: experimental
Version:
Version Date: 2016-12-09

Project
» Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton (E Hux Response to pCO2)
ContributorsAffiliationRole
Olson, Brady M.Western Washington University (WWU)Principal Investigator
Love, BrookeWestern Washington University (WWU)Co-Principal Investigator
Strom, SuzanneWestern Washington University (WWU)Co-Principal Investigator
Still, Kelly AnnWestern Washington University (WWU)Student
Copley, NancyWoods Hole Oceanographic Institution (WHOI BCO-DMO)BCO-DMO Data Manager


Dataset Description

This dataset reports the carbonate chemistry of the Rhodomonas during the time it was used to feed Oxyrrhis in the long term experiments.

Related Reference:
Still, Kelly Ann, Microzooplankton grazing, growth and gross growth efficiency are affected by pCO2 induced changes in phytoplankton biology. (Masters Thesis) Western Washington University. http://cedar.wwu.edu/cgi/viewcontent.cgi?article=1490&context=wwuet


Methods & Sampling

The phytoplankton Rhodomonas sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv).  Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients.  Some of the culture removed was used to evaluate chemical parameters.  Samples for total alkalinity of pre-equilibrated media were taken on growth days 13 and 17 and preserved with HgCl2 and stored at 4° until analysis.  Alkalinity was measured by gran titration using a Titrando 888, and 0.1 N HCl titrant, in a temperature controlled titration vessel.  DIC samples from the long term grazing experiment were filtered through a 0.2 µm nylon syringe filter on the morning of the experimental day, then stored in air tight vials at 4°C until analysis within 60 days using an Apollo SciTech DIC Analyzer AS-C3 which incorporates the LI-7000 CO2/H2O Analyzer. Other parameters were calculated with CO2sys.


Data Processing Description

Data are unprocessed.

BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
- nd (no data) was entered into all blank cells
- replaced spaces with underscores


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Data Files

File
expt5_pCO2_Rh-Ox.csv
(Comma Separated Values (.csv), 3.73 KB)
MD5:e8987cabad0cf232977a0f3b67c9e701
Primary data file for dataset ID 669472

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Parameters

ParameterDescriptionUnits
treatment_rep_culture_dayTreatment replicate that names the sample and the day of semi-continuous culture unitless
alkalinitytotal alkalinity of the culture material removed micromoles/kilogram (umol/kg)
DICdissolved inorganic carbon micromoles/kilogram (umol/kg)
pCO2Partial pressure of carbon dioxide in the water body by computation from pH and alkalinity parts per million by volume (ppmv)


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Instruments

Dataset-specific Instrument Name
LI-7000 CO2/H2O Analyzer
Generic Instrument Name
LI-COR LI-7000 Gas Analyzer
Generic Instrument Description
The LI-7000 CO2/H2O Gas Analyzer is a high performance, dual cell, differential gas analyzer. It was designed to expand on the capabilities of the LI-6262 CO2/ H2O Gas Analyzer. A dichroic beam splitter at the end of the optical path provides radiation to two separate detectors, one filtered to detect radiation absorption of CO2 and the other to detect absorption by H2O. The two separate detectors measure infrared absorption by CO2 and H2O in the same gas stream. The LI-7000 CO2/ H2O Gas Analyzer is a differential analyzer, in which a known concentration (which can be zero) gas is put in the reference cell, and an unknown gas is put in the sample cell.

Dataset-specific Instrument Name
Generic Instrument Name
Automatic titrator
Dataset-specific Description
Titrando 888
Generic Instrument Description
Instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached.

Dataset-specific Instrument Name
Apollo SciTech DIC Analyzer AS-C3
Generic Instrument Name
CO2 Analyzer
Generic Instrument Description
Measures atmospheric carbon dioxide (CO2) concentration.


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Deployments

Lab_Olson_B

Website
Platform
WWU
Start Date
2011-03-31
End Date
2016-09-15
Description
laboratory experiments


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Project Information

Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton (E Hux Response to pCO2)


Description from NSF award abstract:
The calcifying Haptophyte Emiliania huxleyi appears to be acutely sensitive to the rising concentration of ocean pCO2. Documented responses by E. huxleyi to elevated pCO2 include modifications to their calcification rate and cell size, malformation of coccoliths, elevated growth rates, increased organic carbon production, lowering of PIC:POC ratios, and elevated production of the active climate gas DMS. Changes in these parameters are mechanisms known to elicit alterations in grazing behavior by microzooplankton, the oceans dominant grazer functional group. The investigators hypothesize that modifications to the physiology and biochemistry of calcifying and non-calcifying Haptophyte Emiliania huxleyi in response to elevated pCO2 will precipitate alterations in microzooplankton grazing dynamics. To test this hypothesis, they will conduct controlled laboratory experiments where several strains of E. huxleyi are grown at several CO2 concentrations. After careful characterization of the biochemical and physiological responses of the E. huxleyi strains to elevated pCO2, they will provide these strains as food to several ecologically-important microzooplankton and document grazing dynamics. E. huxleyi is an ideal organism for the study of phytoplankton and microzooplankton responses to rising anthropogenic CO2, the effects of which in the marine environment are called ocean acidification; E. huxleyi is biogeochemically important, is well studied, numerous strains are in culture that exhibit variation in the parameters described above, and they are readily fed upon by ecologically important microzooplankton.

The implications of changes in microzooplankton grazing for carbon cycling, specifically CaCO3 export, DMS production, nutrient regeneration in surface waters, and carbon transfer between trophic levels are profound, as this grazing, to a large degree, regulates all these processes. E. huxleyi is a model prey organism because it is one of the most biogeochemically influential global phytoplankton. It forms massive seasonal blooms, contributes significantly to marine inorganic and organic carbon cycles, is a large producer of the climatically active gas DMS, and is a source of organic matter for trophic levels both above and below itself. The planned controlled study will increase our knowledge of the mechanisms that drive patterns of change between trophic levels, thus providing a wider array of tools necessary to understand the complex nature of ocean acidification field studies, where competing variables can confound precise interpretation.



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Funding

Funding SourceAward
NSF Division of Ocean Sciences (NSF OCE)

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