http://lod.bco-dmo.org/id/dataset/669488
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2016-12-09
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Grazing experiment 5: Cellular carbohydrate data for low-high pCO2 acclimated Rhodomonas sp. cultures, 2011-2016 (E Hux Response to pCO2 project)
2016-12-09
publication
2016-12-09
revision
BCO-DMO Linked Data URI
2016-12-09
creation
http://lod.bco-dmo.org/id/dataset/669488
M. Brady Olson
Western Washington University
principalInvestigator
Brooke Love
Western Washington University
principalInvestigator
Suzanne Strom
Western Washington University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Olson, M. Brady, Love, B., Strom, S. (2016) Grazing experiment 5: Cellular carbohydrate data for low-high pCO2 acclimated Rhodomonas sp. cultures, 2011-2016 (E Hux Response to pCO2 project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2016-12-09 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/669488 [access date]
Cellular carbohydrate data for low-high pCO2 acclimated Rhodomonas sp. cultures Dataset Description: <p><strong>Related Reference:&nbsp;</strong><br />
Still, Kelly Ann, Microzooplankton grazing, growth and gross growth efficiency are affected by pCO<sub>2</sub> induced changes in phytoplankton biology. (Masters Thesis) Western Washington University.&nbsp;<a href="http://cedar.wwu.edu/cgi/viewcontent.cgi?article=1490&amp;context=wwuet">http://cedar.wwu.edu/cgi/viewcontent.cgi?article=1490&amp;context=wwuet</a></p> Methods and Sampling: <p>The phytoplankton Rhodomonas sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv).&nbsp; Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients.&nbsp; Some of the culture removed was used to evaluate chemical parameters.&nbsp; Samples for carbohydrate mass were taken by gravity filtering 30 ml of culture through muffled GF/F filters. Filters were wrapped in aluminum foil and stored at -80°C until analysis.&nbsp; Prior to analysis, filters were extracted in 1 ml of 95% H2SO4 and 1 ml of nanopure water in a sonication bath for 30 minutes and then 20 hours at room temperature.&nbsp; After extraction, samples were centrifuged at 10,000 rpm for 6 minutes.&nbsp; 1.6 ml of the extract was combined with 4 ml of concentrated H2SO4 and 0.8 ml of 10% phenol and allowed to react for 30 minutes.&nbsp; Samples were analyzed colorimetrically using a Spec20D+ spectrophotometer at 490 nm and compared with standards made from fructose.&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0961229 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0961229
completed
M. Brady Olson
Western Washington University
(360) 650-7400
1900 Shannon Point Rd.
Anacortes
WA
98221
United States
Brady.Olson@wwu.edu
pointOfContact
Brooke Love
Western Washington University
(360) 650-2894
516 High St. Mail Stop 9181
Bellingham
WA
98225
United States
brooke.love@wwu.edu
pointOfContact
Suzanne Strom
Western Washington University
360-293-2188
Shannon Point Marine Center 1900 Shannon Point Rd
Anacortes
WA
98221
USA
suzanne.strom@wwu.edu
pointOfContact
asNeeded
Unknown
sample
nm490
fructose_1_6ml
fructose_ml
cell_ml
vol_filt_ml
cells_on_filter
cells_per_ml_extract
fructose_ug_cell
fructose_pg_cell
Spec20D+ spectrophotometer
theme
None, User defined
replicate
No BCO-DMO term
carbohydrates
cell_concentration
volume of water filtered
count
featureType
BCO-DMO Standard Parameters
Spectrophotometer
instrument
BCO-DMO Standard Instruments
Lab_Olson_B
service
Deployment Activity
Bellingham, WA
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton
https://osprey.bco-dmo.org/project/517694
Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton
<p><em><strong>Description from NSF award abstract:</strong></em><br />
The calcifying Haptophyte <em>Emiliania huxleyi </em>appears to be acutely sensitive to the rising concentration of ocean pCO2. Documented responses by <em>E. huxleyi </em>to elevated pCO2 include modifications to their calcification rate and cell size, malformation of coccoliths, elevated growth rates, increased organic carbon production, lowering of PIC:POC ratios, and elevated production of the active climate gas DMS. Changes in these parameters are mechanisms known to elicit alterations in grazing behavior by microzooplankton, the oceans dominant grazer functional group. The investigators hypothesize that modifications to the physiology and biochemistry of calcifying and non-calcifying Haptophyte <em>Emiliania huxleyi </em>in response to elevated pCO2 will precipitate alterations in microzooplankton grazing dynamics. To test this hypothesis, they will conduct controlled laboratory experiments where several strains of <em>E. huxleyi</em> are grown at several CO2 concentrations. After careful characterization of the biochemical and physiological responses of the <em>E. huxleyi</em> strains to elevated pCO2, they will provide these strains as food to several ecologically-important microzooplankton and document grazing dynamics.<em> E. huxleyi</em> is an ideal organism for the study of phytoplankton and microzooplankton responses to rising anthropogenic CO2, the effects of which in the marine environment are called ocean acidification; <em>E. huxleyi </em>is biogeochemically important, is well studied, numerous strains are in culture that exhibit variation in the parameters described above, and they are readily fed upon by ecologically important microzooplankton.</p>
<p>The implications of changes in microzooplankton grazing for carbon cycling, specifically CaCO3 export, DMS production, nutrient regeneration in surface waters, and carbon transfer between trophic levels are profound, as this grazing, to a large degree, regulates all these processes. <em>E. huxleyi </em>is a model prey organism because it is one of the most biogeochemically influential global phytoplankton. It forms massive seasonal blooms, contributes significantly to marine inorganic and organic carbon cycles, is a large producer of the climatically active gas DMS, and is a source of organic matter for trophic levels both above and below itself. The planned controlled study will increase our knowledge of the mechanisms that drive patterns of change between trophic levels, thus providing a wider array of tools necessary to understand the complex nature of ocean acidification field studies, where competing variables can confound precise interpretation.</p>
E Hux Response to pCO2
largerWorkCitation
project
eng; USA
biota
oceans
Bellingham, WA
2016-12-09
0
BCO-DMO catalogue of parameters from Grazing experiment 5: Cellular carbohydrate data for low-high pCO2 acclimated Rhodomonas sp. cultures, 2011-2016 (E Hux Response to pCO2 project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/669498.rdf
Name: sample
Units: unitless
Description: <p>sample ID that names the treatment replicate and the day of semi-continuous culture or standard concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/669499.rdf
Name: nm490
Units: unitless?
Description: <p>485 nm wavelength value</p>
http://lod.bco-dmo.org/id/dataset-parameter/669500.rdf
Name: fructose_1_6ml
Units: picograms (pg) per 1.6 ml extract
Description: <p>carbohydrate content in fructose</p>
http://lod.bco-dmo.org/id/dataset-parameter/669501.rdf
Name: fructose_ml
Units: per milliliter
Description: <p>carbohydrate content</p>
http://lod.bco-dmo.org/id/dataset-parameter/669502.rdf
Name: cell_ml
Units: cells/milliliter
Description: <p>cell concentration in the culture</p>
http://lod.bco-dmo.org/id/dataset-parameter/669503.rdf
Name: vol_filt_ml
Units: milliliters
Description: <p>volume of culture filtered for sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/669504.rdf
Name: cells_on_filter
Units: cells
Description: <p>total cells on the filter</p>
http://lod.bco-dmo.org/id/dataset-parameter/669505.rdf
Name: cells_per_ml_extract
Units: milliliters
Description: <p>cells per ml of extract</p>
http://lod.bco-dmo.org/id/dataset-parameter/669506.rdf
Name: fructose_ug_cell
Units: micrograms/cell
Description: <p>carbohydrate as fructose</p>
http://lod.bco-dmo.org/id/dataset-parameter/669507.rdf
Name: fructose_pg_cell
Units: picograms/cell
Description: <p>carbohydrate as fructose</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
1473
https://datadocs.bco-dmo.org/file/EKKyERQHlNRnNl/expt5_carbo.csv
expt5_carbo.csv
Primary data file for dataset ID 669488
download
https://osprey.bco-dmo.org/dataset/669488/data/download
download
onLine
dataset
<p>The phytoplankton Rhodomonas sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv).&nbsp; Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients.&nbsp; Some of the culture removed was used to evaluate chemical parameters.&nbsp; Samples for carbohydrate mass were taken by gravity filtering 30 ml of culture through muffled GF/F filters. Filters were wrapped in aluminum foil and stored at -80°C until analysis.&nbsp; Prior to analysis, filters were extracted in 1 ml of 95% H2SO4 and 1 ml of nanopure water in a sonication bath for 30 minutes and then 20 hours at room temperature.&nbsp; After extraction, samples were centrifuged at 10,000 rpm for 6 minutes.&nbsp; 1.6 ml of the extract was combined with 4 ml of concentrated H2SO4 and 0.8 ml of 10% phenol and allowed to react for 30 minutes.&nbsp; Samples were analyzed colorimetrically using a Spec20D+ spectrophotometer at 490 nm and compared with standards made from fructose.&nbsp;</p>
Specified by the Principal Investigator(s)
<p>Micrograms per ml for carbohydrate were calculated based on standard curves using fructose and were then normalized to per cell based on cell counts for the sample day.</p>
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- nd (no data) was entered into all blank cells</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Spec20D+ spectrophotometer
Spec20D+ spectrophotometer
PI Supplied Instrument Name: Spec20D+ spectrophotometer Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/
Deployment: Lab_Olson_B
Lab_Olson_B
WWU
laboratory
WWU
laboratory