http://lod.bco-dmo.org/id/dataset/669572
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2016-12-09
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Grazing experiments 5: Daily cell count data for low-high pCO2 acclimated Rhodomonas sp. (E Hux Response to pCO2 project)
2016-12-09
publication
2016-12-09
revision
BCO-DMO Linked Data URI
2016-12-09
creation
http://lod.bco-dmo.org/id/dataset/669572
M. Brady Olson
Western Washington University
principalInvestigator
Brooke Love
Western Washington University
principalInvestigator
Suzanne Strom
Western Washington University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Olson, M. Brady, Love, B., Strom, S. (2016) Grazing experiments 5: Daily cell count data for low-high pCO2 acclimated Rhodomonas sp. (E Hux Response to pCO2 project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2016-12-09 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/669572 [access date]
Daily cell count data for low-high pCO2 acclimated Rhodomonas sp. Dataset Description: <p><strong>Related Reference:</strong><br />
Still, Kelly Ann, Microzooplankton grazing, growth and gross growth efficiency are affected by pCO<sub>2</sub> induced changes in phytoplankton biology. (Masters Thesis) Western Washington University. <a href="http://cedar.wwu.edu/cgi/viewcontent.cgi?article=1490&amp;context=wwuet">http://cedar.wwu.edu/cgi/viewcontent.cgi?article=1490&amp;context=wwuet</a></p> Methods and Sampling: <p>The phytoplankton Rhodomonas sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv). Cultures were started and allowed to grow four days to reach a density of approximately 50,000 cells per ml. Cultures were then diluted daily with pre-equilibrated media containing f/50 nutrients. Each morning of dilution the cultures were gently mixed prior to a small sample being taken for cell counts. Cells were counted live on a Z2 Coulter Particle Counter. The dilution volume was then calculated to achieve a cell density of approximately 25,000 cells per ml which was the density determined in preliminary experiments to be adequate to maintain pCO2 near target concentrations.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0961229 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0961229
completed
M. Brady Olson
Western Washington University
(360) 650-7400
1900 Shannon Point Rd.
Anacortes
WA
98221
United States
Brady.Olson@wwu.edu
pointOfContact
Brooke Love
Western Washington University
(360) 650-2894
516 High St. Mail Stop 9181
Bellingham
WA
98225
United States
brooke.love@wwu.edu
pointOfContact
Suzanne Strom
Western Washington University
360-293-2188
Shannon Point Marine Center 1900 Shannon Point Rd
Anacortes
WA
98221
USA
suzanne.strom@wwu.edu
pointOfContact
asNeeded
Unknown
treatment_replicate
target_density_day_1
count_day_2
day_2_post_dilution
count_day_3
day_3_post_dilution
count_day_4
day_4_post_dilution
count_day_5
day_5_post_dilution
count_day_6
day_6_post_dilution
count_day_7
day_7_post_dilution
count_day_8
day_8_post_dilution
count_day_9
day_9_post_dilution
count_day_10
day_10_post_dilution
count_day_11
day_11_post_dilution
count_day_12
day_12_post_dilution
count_day_13
day_13_post_dilution
count_day_14
day_14_post_dilution
count_day_15
day_15_post_dilution
count_day_16
day_16_post_dilution
count_day_17
Z2 Coulter Particle Counter
theme
None, User defined
sample identification
count
featureType
BCO-DMO Standard Parameters
Coulter Counter
instrument
BCO-DMO Standard Instruments
Lab_Olson_B
service
Deployment Activity
Bellingham, WA
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton
https://osprey.bco-dmo.org/project/517694
Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton
<p><em><strong>Description from NSF award abstract:</strong></em><br />
The calcifying Haptophyte <em>Emiliania huxleyi </em>appears to be acutely sensitive to the rising concentration of ocean pCO2. Documented responses by <em>E. huxleyi </em>to elevated pCO2 include modifications to their calcification rate and cell size, malformation of coccoliths, elevated growth rates, increased organic carbon production, lowering of PIC:POC ratios, and elevated production of the active climate gas DMS. Changes in these parameters are mechanisms known to elicit alterations in grazing behavior by microzooplankton, the oceans dominant grazer functional group. The investigators hypothesize that modifications to the physiology and biochemistry of calcifying and non-calcifying Haptophyte <em>Emiliania huxleyi </em>in response to elevated pCO2 will precipitate alterations in microzooplankton grazing dynamics. To test this hypothesis, they will conduct controlled laboratory experiments where several strains of <em>E. huxleyi</em> are grown at several CO2 concentrations. After careful characterization of the biochemical and physiological responses of the <em>E. huxleyi</em> strains to elevated pCO2, they will provide these strains as food to several ecologically-important microzooplankton and document grazing dynamics.<em> E. huxleyi</em> is an ideal organism for the study of phytoplankton and microzooplankton responses to rising anthropogenic CO2, the effects of which in the marine environment are called ocean acidification; <em>E. huxleyi </em>is biogeochemically important, is well studied, numerous strains are in culture that exhibit variation in the parameters described above, and they are readily fed upon by ecologically important microzooplankton.</p>
<p>The implications of changes in microzooplankton grazing for carbon cycling, specifically CaCO3 export, DMS production, nutrient regeneration in surface waters, and carbon transfer between trophic levels are profound, as this grazing, to a large degree, regulates all these processes. <em>E. huxleyi </em>is a model prey organism because it is one of the most biogeochemically influential global phytoplankton. It forms massive seasonal blooms, contributes significantly to marine inorganic and organic carbon cycles, is a large producer of the climatically active gas DMS, and is a source of organic matter for trophic levels both above and below itself. The planned controlled study will increase our knowledge of the mechanisms that drive patterns of change between trophic levels, thus providing a wider array of tools necessary to understand the complex nature of ocean acidification field studies, where competing variables can confound precise interpretation.</p>
E Hux Response to pCO2
largerWorkCitation
project
eng; USA
biota
oceans
Bellingham, WA
2016-12-09
0
BCO-DMO catalogue of parameters from Grazing experiments 5: Daily cell count data for low-high pCO2 acclimated Rhodomonas sp. (E Hux Response to pCO2 project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/669582.rdf
Name: treatment_replicate
Units: unitless
Description: <p>sample identifier: individual grazer analyzed</p>
http://lod.bco-dmo.org/id/dataset-parameter/669583.rdf
Name: target_density_day_1
Units: cells/milliliter
Description: <p>Target count for day 1 morning cell count</p>
http://lod.bco-dmo.org/id/dataset-parameter/669584.rdf
Name: count_day_2
Units: cells/milliliter
Description: <p>Count for day 2 morning cell count</p>
http://lod.bco-dmo.org/id/dataset-parameter/669585.rdf
Name: day_2_post_dilution
Units: cells/milliliter
Description: <p>Count for day 2 post-dilution; for the predicted cell count after dilution</p>
http://lod.bco-dmo.org/id/dataset-parameter/669586.rdf
Name: count_day_3
Units: cells/milliliter
Description: <p>Count for day 3 morning cell count</p>
http://lod.bco-dmo.org/id/dataset-parameter/669587.rdf
Name: day_3_post_dilution
Units: cells/milliliter
Description: <p>Count for day 3 post-dilution; for the predicted cell count after dilution</p>
http://lod.bco-dmo.org/id/dataset-parameter/669588.rdf
Name: count_day_4
Units: cells/milliliter
Description: <p>Count for day 4 morning cell count</p>
http://lod.bco-dmo.org/id/dataset-parameter/669589.rdf
Name: day_4_post_dilution
Units: cells/milliliter
Description: <p>Count for day 4 post-dilution; for the predicted cell count after dilution</p>
http://lod.bco-dmo.org/id/dataset-parameter/669590.rdf
Name: count_day_5
Units: cells/milliliter
Description: <p>Count for day 5 morning cell count</p>
http://lod.bco-dmo.org/id/dataset-parameter/669591.rdf
Name: day_5_post_dilution
Units: cells/milliliter
Description: <p>Count for day 5 post-dilution; for the predicted cell count after dilution</p>
http://lod.bco-dmo.org/id/dataset-parameter/669592.rdf
Name: count_day_6
Units: cells/milliliter
Description: <p>Count for day 6 morning cell count</p>
http://lod.bco-dmo.org/id/dataset-parameter/669593.rdf
Name: day_6_post_dilution
Units: cells/milliliter
Description: <p>Count for day 6 post-dilution; for the predicted cell count after dilution</p>
http://lod.bco-dmo.org/id/dataset-parameter/669594.rdf
Name: count_day_7
Units: cells/milliliter
Description: <p>Count for day 7 morning cell count</p>
http://lod.bco-dmo.org/id/dataset-parameter/669595.rdf
Name: day_7_post_dilution
Units: cells/milliliter
Description: <p>Count for day 7 post-dilution; for the predicted cell count after dilution</p>
http://lod.bco-dmo.org/id/dataset-parameter/669596.rdf
Name: count_day_8
Units: cells/milliliter
Description: <p>Count for day 8 morning cell count</p>
http://lod.bco-dmo.org/id/dataset-parameter/669597.rdf
Name: day_8_post_dilution
Units: cells/milliliter
Description: <p>Count for day 8 post-dilution; for the predicted cell count after dilution</p>
http://lod.bco-dmo.org/id/dataset-parameter/669598.rdf
Name: count_day_9
Units: cells/milliliter
Description: <p>Count for day 9 morning cell count</p>
http://lod.bco-dmo.org/id/dataset-parameter/669599.rdf
Name: day_9_post_dilution
Units: cells/milliliter
Description: <p>Count for day 9 post-dilution; for the predicted cell count after dilution</p>
http://lod.bco-dmo.org/id/dataset-parameter/669600.rdf
Name: count_day_10
Units: cells/milliliter
Description: <p>Count for day 10 morning cell count</p>
http://lod.bco-dmo.org/id/dataset-parameter/669601.rdf
Name: day_10_post_dilution
Units: cells/milliliter
Description: <p>Count for day 10 post-dilution; for the predicted cell count after dilution</p>
http://lod.bco-dmo.org/id/dataset-parameter/669602.rdf
Name: count_day_11
Units: cells/milliliter
Description: <p>Count for day 11 morning cell count</p>
http://lod.bco-dmo.org/id/dataset-parameter/669603.rdf
Name: day_11_post_dilution
Units: cells/milliliter
Description: <p>Count for day 11 post-dilution; for the predicted cell count after dilution</p>
http://lod.bco-dmo.org/id/dataset-parameter/669604.rdf
Name: count_day_12
Units: cells/milliliter
Description: <p>Count for day 12 morning cell count</p>
http://lod.bco-dmo.org/id/dataset-parameter/669605.rdf
Name: day_12_post_dilution
Units: cells/milliliter
Description: <p>Count for day 12 post-dilution; for the predicted cell count after dilution</p>
http://lod.bco-dmo.org/id/dataset-parameter/669606.rdf
Name: count_day_13
Units: cells/milliliter
Description: <p>Count for day 13 morning cell count</p>
http://lod.bco-dmo.org/id/dataset-parameter/669607.rdf
Name: day_13_post_dilution
Units: cells/milliliter
Description: <p>Count for day 13 post-dilution; for the predicted cell count after dilution</p>
http://lod.bco-dmo.org/id/dataset-parameter/669608.rdf
Name: count_day_14
Units: cells/milliliter
Description: <p>Count for day 14 morning cell count</p>
http://lod.bco-dmo.org/id/dataset-parameter/669609.rdf
Name: day_14_post_dilution
Units: cells/milliliter
Description: <p>Count for day 14 post-dilution; for the predicted cell count after dilution</p>
http://lod.bco-dmo.org/id/dataset-parameter/669610.rdf
Name: count_day_15
Units: cells/milliliter
Description: <p>Count for day 15 morning cell count</p>
http://lod.bco-dmo.org/id/dataset-parameter/669611.rdf
Name: day_15_post_dilution
Units: cells/milliliter
Description: <p>Count for day 15 post-dilution; for the predicted cell count after dilution</p>
http://lod.bco-dmo.org/id/dataset-parameter/669612.rdf
Name: count_day_16
Units: cells/milliliter
Description: <p>Count for day 16 morning cell count</p>
http://lod.bco-dmo.org/id/dataset-parameter/669613.rdf
Name: day_16_post_dilution
Units: cells/milliliter
Description: <p>Count for day 16 post-dilution; for the predicted cell count after dilution</p>
http://lod.bco-dmo.org/id/dataset-parameter/669614.rdf
Name: count_day_17
Units: cells/milliliter
Description: <p>Count for day 17 morning cell count</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2360
https://datadocs.bco-dmo.org/file/8XXgn2zsQorJRX/expt5_cell_count_daily.csv
expt5_cell_count_daily.csv
Primary data file for dataset ID 669572
download
https://osprey.bco-dmo.org/dataset/669572/data/download
download
onLine
dataset
<p>The phytoplankton Rhodomonas sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv). Cultures were started and allowed to grow four days to reach a density of approximately 50,000 cells per ml. Cultures were then diluted daily with pre-equilibrated media containing f/50 nutrients. Each morning of dilution the cultures were gently mixed prior to a small sample being taken for cell counts. Cells were counted live on a Z2 Coulter Particle Counter. The dilution volume was then calculated to achieve a cell density of approximately 25,000 cells per ml which was the density determined in preliminary experiments to be adequate to maintain pCO2 near target concentrations.</p>
Specified by the Principal Investigator(s)
<p>These data are unprocessed cell counts.</p>
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Z2 Coulter Particle Counter
Z2 Coulter Particle Counter
PI Supplied Instrument Name: Z2 Coulter Particle Counter PI Supplied Instrument Description:Used to count cells Instrument Name: Coulter Counter Instrument Short Name: Instrument Description: An apparatus for counting and sizing particles suspended in electrolytes. It is used for cells, bacteria, prokaryotic cells and virus particles. A typical Coulter counter has one or more microchannels that separate two chambers containing electrolyte solutions.
from https://en.wikipedia.org/wiki/Coulter_counter
Deployment: Lab_Olson_B
Lab_Olson_B
WWU
laboratory
WWU
laboratory