http://lod.bco-dmo.org/id/dataset/669693
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2016-12-09
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Raw concentrations of individual PLFA compounds from Massachusetts from 2012-2015.
2016-12-08
publication
2016-12-08
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-04-05
publication
https://doi.org/10.1575/1912/bco-dmo.669693.1
Amanda Spivak
Woods Hole Oceanographic Institution
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Spivak, A. (2016) Raw concentrations of individual PLFA compounds from Massachusetts from 2012-2015. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2016-12-08 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.669693.1 [access date]
Raw concentrations of individual PLFA compounds. Dataset Description: <p>We conducted two sets of&nbsp;13C label addition experiments. In one set, we evaluated how nutrient fertilization affected bacterial utilization of BMA carbon over a growing season. In the other set, we used&nbsp;13C-labelled&nbsp;S.&nbsp;alterniflora&nbsp;to assess bacterial incorporation of recently-produced macrophyte detritus.&nbsp;Data presented are raw concentrations individual PLFA compounds (ng g-1&nbsp;dry sediment) measured in surface sediments.</p> Methods and Sampling: <p><strong>Methodology from&nbsp;Spivak, AC and J Ossolinski. 2016. Limited effects of nutrient enrichment on bacterial carbon sources in salt marsh tidal creek sediments. Marine Ecology Progress Series. 544:107-130. 10.3354/meps11587</strong></p>
<p>In June, August, and October 2013, intact sediment cores were collected from the mudflats of the fertilized and reference creeks at low tide (n=3 per creek in June and October, n=6 per creek in August; 31 cm diameter x 15 cm deep). These time periods reflected late spring, late summer, and early fall conditions. Sediment cores were transported to Woods Hole Oceanographic Institution’s mesocosm system (Woods Hole, MA). Cores were placed in rectangular fiberglass tanks (2.7 m x 1.2 m x 0.8 m, l x w x d) that served as a water bath to minimize extreme fluctuations in day – night temperatures. The mesocosm system is located outside, so cores experienced ambient weather conditions.</p>
<p>Upon placement in the fiberglass tanks, the overlying water collected with each sediment core was continuously recirculated (~9 cm deep). The cores acclimated to the mesocosm system for 1 – 3 days, depending on weather, as we applied the&nbsp;13C label during sunny periods when BMA would be productive. Visible epifauna (e.g., snails, shrimp) were removed to minimize grazing on benthic microbes. At the beginning of each experiment, the overlying water column was removed and replaced with filtered water from the creek where the core was collected. We used 0.2 um filtered creek water to minimize label uptake by water column microbes and recirculated the water column to maintain well-mixed conditions. The isotopic label was added as&nbsp;13C-sodium bicarbonate (NaHCO3, 99 atom %, Sigma-Aldrich) to the water column of each core in June and October and half of the cores from each creek in August. The other half of the August cores received&nbsp;13C-labeled&nbsp;S. alterniflora&nbsp;detritus. This material was produced from a separate experiment in which living&nbsp;S. alterniflora&nbsp;plants from PIE-LTER were dosed with&nbsp;13CO2&nbsp;for 3 h (Spivak &amp; Reeve 2015). Aboveground leaves were harvested after label exposure, dried (60 deg C), and ground into a coarse powder that was evenly applied across the sediment surface.&nbsp;13C-S. alterniflora&nbsp;was only applied in August due to availability of the labeled material. From here forward, experiments receiving&nbsp;13C-NaHCO3&nbsp;or&nbsp;13C-S. alterniflora&nbsp;are referred to as BMA or&nbsp;S. alterniflora&nbsp;experiments, respectively, to reflect the autotrophic source of carbon to bacteria. On average (+/- standard error, S.E.), the cores received 11.90 +/- 0.07 mg, 10.90 +/- 0.05, and 10.97 +/- 0.09 mg&nbsp;13C from NaHCO3&nbsp;application in June, August, and October, respectively, and 3.17 +/- 0.12 mg&nbsp;13C from the&nbsp;S. alterniflora&nbsp;detritus in August. The&nbsp;13C label, as NaHCO3&nbsp;or&nbsp;S. alterniflora, was applied for four hours between 11:00 – 15:00 h before the overlying water was removed and the cores were rinsed with at least three volumes of filtered creek water to remove unused label. The four hour sampling period was based on results from a preliminary study demonstrating that this timeframe was sufficient for detecting the label in algal and bacterial lipids. After the final rinse, the overlying water column was replaced with filtered creek water and recirculated for the duration of the experiments.&nbsp;</p>
<p>Sediment samples for organic matter composition were collected by placing a hard plastic sleeve around a polyvinyl chloride (PVC) corer (5 cm diameter x 15 cm deep) and then removing the corer. The plastic sleeve remained in place to maintain the integrity of the sediment column and mark the core location (Spivak 2015). The top 0.5 cm of each core was collected into pre-combusted vials and frozen (-80 deg C) until analysis for total organic carbon and nitrogen content and stable isotopes (d13C, d15N) and lipid biomarker composition. Adjacent samples for benthic chlorophyll were collected with smaller cores (1.5 cm diameter x 1 cm deep) into glass vials and frozen (-20 deg C) until analysis. Additional sediment cores for organic matter composition and benthic chlorophyll were collected 4, 8, 24, and 48 h after the&nbsp;13C-labeled NaHCO3&nbsp;was applied in June, August, and October and 4, 8, 24, and 144h after the&nbsp;13C-labeled&nbsp;S. alterniflora&nbsp;was applied in August.&nbsp;</p>
<p>Lipid biomarker compounds were extracted using a modified Bligh and Dyer (1959) method. Sediment samples were extracted with a chloroform : methylene chloride : phosphate buffer saline mixture (2:1:0.8, v:v:v) using a microwave-accelerated reaction system (MARS6); samples were heated to 80 deg C for 10 min with continuous stirring. Following extraction, samples were partitioned and the organic phase was removed. The total lipid extract was concentrated under N2&nbsp;and samples were separated on silica gel columns by eluting with chloroform, acetone (F1/2), and methanol (F3) (Guckert et al. 1985). The F3 (phospholipids) was dried under N2&nbsp;and saponified with 0.5 M NaOH at 70 deg C for 4 h. Saponified samples were acidified and extracted three times with hexane. The extract was methylated with acidic methanol (95:5 methanol: HCl) and heated overnight at 70 deg C to form fatty acid methyl esters (FAME). Samples were analyzed with an Agilent 7890 gas chromatograph with an effluent split ~70:30 between a 5975C mass spectrometer and a flame ionization detector. Peaks were separated on an Agilent DB-5 ms column (60 m, 0.25 mm inner diameter, 0.25 um film). FAME concentrations were quantified using methyl heneicosanoate as an internal standard. FAs are designated A:BwC, where A is the number of carbon atoms, B is the number of double bonds, and C is the position of the first double bond from the aliphatic ‘w’ end of the molecule. The prefixes ‘i’ and ‘a’ refer to iso and anteiso methyl branched FAs and indicate whether the methyl group is attached to the penultimate or antepenulttimate carbon atoms (Bianchi &amp; Canuel 2011).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1233678 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1233678
completed
Amanda Spivak
Woods Hole Oceanographic Institution
706-542-5709
Marine Science Rm. 164 Department of Marine Science
Athens
GA
30602
United States
aspivak@uga.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
month
estuary
timepoint
experiment
c12
i13
a13
c13
i14
c14
i15
a15
c15
i16
c16
me10_16
i17
a17
c17
c18
i19
a19
c19
c20_5w3
c20_4w6
c20
c21
unsatC22
c22_6w3
c22_5
c22
c23
c24
c26
c28
totalFA
Flame ionization detector
Agilent 7890 gas chromatograph
5975C mass spectrometer
Core
theme
None, User defined
month of year
site
time of day
treatment
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
Flame Ionization Detector
Gas Chromatograph
Mass Spectrometer
Push Corer
instrument
BCO-DMO Standard Instruments
Spivak_2012
service
Deployment Activity
Rowley and Woods Hole, MA
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Eutrophication Effects on Sediment Metabolism and Benthic Algal-bacterial Coupling: An Application of Novel Techniques in a LTER Estuary
https://osprey.bco-dmo.org/project/529583
Eutrophication Effects on Sediment Metabolism and Benthic Algal-bacterial Coupling: An Application of Novel Techniques in a LTER Estuary
<p><em>Extracted from the NSF award abstract:</em><br />
This project will address how rates of benthic microalgal production respond to eutrophication and geomorphological changes in human-impacted tidal creeks. Excess nutrient loading increases benthic algal biomass and likely stimulates production rates but the magnitude of nutrient and geomorphological effects on rates of production is unknown. Will changes in benthic algal productivity affect algal-bacterial coupling? Furthermore, how is algal-bacterial coupling affected by geomorphological changes, which may be exacerbated by excess nutrient loading but can also occur in pristine marshes?</p>
<p>This project will take advantage of the infrastructure of the TIDE project, a long-term saltmarsh eutrophication experiment at the Plum Island Ecosystem - Long Term Ecological Research site in Northeastern Massachusetts. Specifically, the PIs will measure benthic metabolism and examine algal- bacterial coupling in fertilized and ambient nutrient tidal creeks in the first field season. The following field season, they will compare sediment metabolism and carbon dynamics on slumped tidal creek walls (i.e. areas where low marsh has collapsed into the tidal creek) to that on the bottom of tidal creeks. In both years, gross and net production will be determined using an innovative triple oxygen isotope technique and traditional dissolved oxygen and inorganic carbon flux measurements. Comparisons between these methods will be useful in informing studies of sediment metabolism. Lipid biomarkers will be used to characterize the sources of organic matter to creek sediments, and stable isotope analysis of bacterial specific biomarkers to identify the sources of organic carbon utilized by sediment bacteria. The biomarkers will reveal whether sediment bacteria use organic matter substrates, such as benthic microalgal carbon, selectively or in proportion to availability. Overall, results from the proposed study will provide important information about how sediment carbon dynamics in shallow tidal creeks respond to long term eutrophication. Furthermore, findings will enhance understanding of the role of tidal creeks in coastal biogeochemistry.</p>
Benthic_PP_at_TIDE
largerWorkCitation
project
eng; USA
oceans
Rowley and Woods Hole, MA
2016-12-08
Plum Island Estuary, Rowley Massachusetts
0
BCO-DMO catalogue of parameters from Raw concentrations of individual PLFA compounds from Massachusetts from 2012-2015.
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/669701.rdf
Name: month
Units: unitless
Description: <p>Month samples were collected; mm</p>
http://lod.bco-dmo.org/id/dataset-parameter/669702.rdf
Name: estuary
Units: unitless
Description: <p>The core originiated from Sweeny or West tidal creeks</p>
http://lod.bco-dmo.org/id/dataset-parameter/669703.rdf
Name: timepoint
Units: unitless
Description: <p>Timepoint refers to when the sample was collected before (PL) or after the 13C-isotope label was added; HH:MM</p>
http://lod.bco-dmo.org/id/dataset-parameter/669704.rdf
Name: experiment
Units: unitless
Description: <p>Experiment refers to whether the 13C label was applied as benthic microalgae (BMA) or Spartina alterniflora (salt) detritus.</p>
http://lod.bco-dmo.org/id/dataset-parameter/669705.rdf
Name: c12
Units: percentage
Description: <p>Concentration of a combination of algae and microbes; short chain fatty acid</p>
http://lod.bco-dmo.org/id/dataset-parameter/669706.rdf
Name: i13
Units: percentage
Description: <p>iso methyl branced FA; indicates whether the methyl group is attached to the penultimate carbon atom</p>
http://lod.bco-dmo.org/id/dataset-parameter/669707.rdf
Name: a13
Units: percentage
Description: <p>anteiso methyl branced FAs; indicates whether methyl group is attached to the antepenultimate carbon atom</p>
http://lod.bco-dmo.org/id/dataset-parameter/669708.rdf
Name: c13
Units: percentage
Description: <p>Concentration of a combination of algae and microbes; short chain fatty acid</p>
http://lod.bco-dmo.org/id/dataset-parameter/669709.rdf
Name: i14
Units: percentage
Description: <p>iso methyl branced FA; indicates whether the methyl group is attached to the penultimate carbon atom</p>
http://lod.bco-dmo.org/id/dataset-parameter/669710.rdf
Name: c14
Units: percentage
Description: <p>Conentration of carbon isotope</p>
http://lod.bco-dmo.org/id/dataset-parameter/669711.rdf
Name: i15
Units: percentage
Description: <p>iso methyl branced FA; indicates whether the methyl group is attached to the penultimate carbon atom</p>
http://lod.bco-dmo.org/id/dataset-parameter/669712.rdf
Name: a15
Units: percentage
Description: <p>anteiso methyl branced FAs; indicates whether methyl group is attached to the antepenultimate carbon atom</p>
http://lod.bco-dmo.org/id/dataset-parameter/669713.rdf
Name: c15
Units: percentage
Description: <p>Concentration of carbon isotope</p>
http://lod.bco-dmo.org/id/dataset-parameter/669714.rdf
Name: i16
Units: percentage
Description: <p>iso methyl branced FA; indicates whether the methyl group is attached to the penultimate carbon atom</p>
http://lod.bco-dmo.org/id/dataset-parameter/669715.rdf
Name: c16
Units: percentage
Description: <p>Concentration of sulfate reducing bacteria</p>
http://lod.bco-dmo.org/id/dataset-parameter/669716.rdf
Name: me10_16
Units: percentage
Description: <p>Concentration of sulfate reducing bacteria</p>
http://lod.bco-dmo.org/id/dataset-parameter/669717.rdf
Name: i17
Units: percentage
Description: <p>iso methyl branced FA; indicates whether the methyl group is attached to the penultimate carbon atom</p>
http://lod.bco-dmo.org/id/dataset-parameter/669718.rdf
Name: a17
Units: percentage
Description: <p>anteiso methyl branced FAs; indicates whether methyl group is attached to the antepenultimate carbon atom</p>
http://lod.bco-dmo.org/id/dataset-parameter/669719.rdf
Name: c17
Units: percentage
Description: <p>Concentration of carbon isotope</p>
http://lod.bco-dmo.org/id/dataset-parameter/669720.rdf
Name: c18
Units: percentage
Description: <p>Concentration of carbon isotope</p>
http://lod.bco-dmo.org/id/dataset-parameter/669721.rdf
Name: i19
Units: percentage
Description: <p>iso methyl branced FA; indicates whether the methyl group is attached to the penultimate carbon atom</p>
http://lod.bco-dmo.org/id/dataset-parameter/669722.rdf
Name: a19
Units: percentage
Description: <p>anteiso methyl branced FAs; indicates whether methyl group is attached to the antepenultimate carbon atom</p>
http://lod.bco-dmo.org/id/dataset-parameter/669723.rdf
Name: c19
Units: percentage
Description: <p>Concentration of carbon isotope</p>
http://lod.bco-dmo.org/id/dataset-parameter/669724.rdf
Name: c20_5w3
Units: percentage
Description: <p>Isotopic composition of compounds and subclasses representing algae; polyunsaturated fatty acids</p>
http://lod.bco-dmo.org/id/dataset-parameter/669725.rdf
Name: c20_4w6
Units: percentage
Description: <p>Isotopic composition of compounds and subclasses representing algae; polyunsaturated fatty acids</p>
http://lod.bco-dmo.org/id/dataset-parameter/669726.rdf
Name: c20
Units: percentage
Description: <p>Concentration of carbon isotope</p>
http://lod.bco-dmo.org/id/dataset-parameter/669727.rdf
Name: c21
Units: percentage
Description: <p>Concentration of carbon isotope</p>
http://lod.bco-dmo.org/id/dataset-parameter/669728.rdf
Name: unsatC22
Units: percentage
Description: <p>Concentration of unsaturated fatty acids</p>
http://lod.bco-dmo.org/id/dataset-parameter/669729.rdf
Name: c22_6w3
Units: percentage
Description: <p>Isotopic composition of compounds and subclasses representing algae; polyunsaturated fatty acids</p>
http://lod.bco-dmo.org/id/dataset-parameter/669730.rdf
Name: c22_5
Units: percentage
Description: <p>Isotopic composition of compounds and subclasses representing algae; polyunsaturated fatty acids</p>
http://lod.bco-dmo.org/id/dataset-parameter/669731.rdf
Name: c22
Units: percentage
Description: <p>Concentration of carbon isotope</p>
http://lod.bco-dmo.org/id/dataset-parameter/669732.rdf
Name: c23
Units: percentage
Description: <p>Concentration of carbon isotope</p>
http://lod.bco-dmo.org/id/dataset-parameter/669733.rdf
Name: c24
Units: percentage
Description: <p>Concentration of carbon isotope</p>
http://lod.bco-dmo.org/id/dataset-parameter/669734.rdf
Name: c26
Units: percentage
Description: <p>Concentration of carbon isotope</p>
http://lod.bco-dmo.org/id/dataset-parameter/669735.rdf
Name: c28
Units: percentage
Description: <p>Concentration of carbon isotope</p>
http://lod.bco-dmo.org/id/dataset-parameter/669736.rdf
Name: totalFA
Units: percentage
Description: <p>Total fatty acid concentration</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
40123
https://darchive.mblwhoilibrary.org/bitstream/1912/23976/1/dataset-669693_sediment-plfa-concentrations__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.669693.1
download
onLine
dataset
<p><strong>Methodology from&nbsp;Spivak, AC and J Ossolinski. 2016. Limited effects of nutrient enrichment on bacterial carbon sources in salt marsh tidal creek sediments. Marine Ecology Progress Series. 544:107-130. 10.3354/meps11587</strong></p>
<p>In June, August, and October 2013, intact sediment cores were collected from the mudflats of the fertilized and reference creeks at low tide (n=3 per creek in June and October, n=6 per creek in August; 31 cm diameter x 15 cm deep). These time periods reflected late spring, late summer, and early fall conditions. Sediment cores were transported to Woods Hole Oceanographic Institution’s mesocosm system (Woods Hole, MA). Cores were placed in rectangular fiberglass tanks (2.7 m x 1.2 m x 0.8 m, l x w x d) that served as a water bath to minimize extreme fluctuations in day – night temperatures. The mesocosm system is located outside, so cores experienced ambient weather conditions.</p>
<p>Upon placement in the fiberglass tanks, the overlying water collected with each sediment core was continuously recirculated (~9 cm deep). The cores acclimated to the mesocosm system for 1 – 3 days, depending on weather, as we applied the&nbsp;13C label during sunny periods when BMA would be productive. Visible epifauna (e.g., snails, shrimp) were removed to minimize grazing on benthic microbes. At the beginning of each experiment, the overlying water column was removed and replaced with filtered water from the creek where the core was collected. We used 0.2 um filtered creek water to minimize label uptake by water column microbes and recirculated the water column to maintain well-mixed conditions. The isotopic label was added as&nbsp;13C-sodium bicarbonate (NaHCO3, 99 atom %, Sigma-Aldrich) to the water column of each core in June and October and half of the cores from each creek in August. The other half of the August cores received&nbsp;13C-labeled&nbsp;S. alterniflora&nbsp;detritus. This material was produced from a separate experiment in which living&nbsp;S. alterniflora&nbsp;plants from PIE-LTER were dosed with&nbsp;13CO2&nbsp;for 3 h (Spivak &amp; Reeve 2015). Aboveground leaves were harvested after label exposure, dried (60 deg C), and ground into a coarse powder that was evenly applied across the sediment surface.&nbsp;13C-S. alterniflora&nbsp;was only applied in August due to availability of the labeled material. From here forward, experiments receiving&nbsp;13C-NaHCO3&nbsp;or&nbsp;13C-S. alterniflora&nbsp;are referred to as BMA or&nbsp;S. alterniflora&nbsp;experiments, respectively, to reflect the autotrophic source of carbon to bacteria. On average (+/- standard error, S.E.), the cores received 11.90 +/- 0.07 mg, 10.90 +/- 0.05, and 10.97 +/- 0.09 mg&nbsp;13C from NaHCO3&nbsp;application in June, August, and October, respectively, and 3.17 +/- 0.12 mg&nbsp;13C from the&nbsp;S. alterniflora&nbsp;detritus in August. The&nbsp;13C label, as NaHCO3&nbsp;or&nbsp;S. alterniflora, was applied for four hours between 11:00 – 15:00 h before the overlying water was removed and the cores were rinsed with at least three volumes of filtered creek water to remove unused label. The four hour sampling period was based on results from a preliminary study demonstrating that this timeframe was sufficient for detecting the label in algal and bacterial lipids. After the final rinse, the overlying water column was replaced with filtered creek water and recirculated for the duration of the experiments.&nbsp;</p>
<p>Sediment samples for organic matter composition were collected by placing a hard plastic sleeve around a polyvinyl chloride (PVC) corer (5 cm diameter x 15 cm deep) and then removing the corer. The plastic sleeve remained in place to maintain the integrity of the sediment column and mark the core location (Spivak 2015). The top 0.5 cm of each core was collected into pre-combusted vials and frozen (-80 deg C) until analysis for total organic carbon and nitrogen content and stable isotopes (d13C, d15N) and lipid biomarker composition. Adjacent samples for benthic chlorophyll were collected with smaller cores (1.5 cm diameter x 1 cm deep) into glass vials and frozen (-20 deg C) until analysis. Additional sediment cores for organic matter composition and benthic chlorophyll were collected 4, 8, 24, and 48 h after the&nbsp;13C-labeled NaHCO3&nbsp;was applied in June, August, and October and 4, 8, 24, and 144h after the&nbsp;13C-labeled&nbsp;S. alterniflora&nbsp;was applied in August.&nbsp;</p>
<p>Lipid biomarker compounds were extracted using a modified Bligh and Dyer (1959) method. Sediment samples were extracted with a chloroform : methylene chloride : phosphate buffer saline mixture (2:1:0.8, v:v:v) using a microwave-accelerated reaction system (MARS6); samples were heated to 80 deg C for 10 min with continuous stirring. Following extraction, samples were partitioned and the organic phase was removed. The total lipid extract was concentrated under N2&nbsp;and samples were separated on silica gel columns by eluting with chloroform, acetone (F1/2), and methanol (F3) (Guckert et al. 1985). The F3 (phospholipids) was dried under N2&nbsp;and saponified with 0.5 M NaOH at 70 deg C for 4 h. Saponified samples were acidified and extracted three times with hexane. The extract was methylated with acidic methanol (95:5 methanol: HCl) and heated overnight at 70 deg C to form fatty acid methyl esters (FAME). Samples were analyzed with an Agilent 7890 gas chromatograph with an effluent split ~70:30 between a 5975C mass spectrometer and a flame ionization detector. Peaks were separated on an Agilent DB-5 ms column (60 m, 0.25 mm inner diameter, 0.25 um film). FAME concentrations were quantified using methyl heneicosanoate as an internal standard. FAs are designated A:BwC, where A is the number of carbon atoms, B is the number of double bonds, and C is the position of the first double bond from the aliphatic ‘w’ end of the molecule. The prefixes ‘i’ and ‘a’ refer to iso and anteiso methyl branched FAs and indicate whether the methyl group is attached to the penultimate or antepenulttimate carbon atoms (Bianchi &amp; Canuel 2011).</p>
Specified by the Principal Investigator(s)
<p>The file includes raw data only.</p>
<p><strong>BCO-DMO Data Processing Notes:</strong></p>
<p><span style="color:rgb(0, 0, 0); font-family:helvetica,arial,sans-serif; font-size:14.4px">-reformatted column names to comply with BCO-DMO standards.</span><br />
<span style="color:rgb(0, 0, 0); font-family:helvetica,arial,sans-serif; font-size:14.4px">-displayed months numerically</span></p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Flame ionization detector
Flame ionization detector
PI Supplied Instrument Name: Flame ionization detector PI Supplied Instrument Description:Analyzed samples Instrument Name: Flame Ionization Detector Instrument Short Name:FID Instrument Description: A flame ionization detector (FID) is a scientific instrument that measures the concentration of organic species in a gas stream. It is frequently used as a detector in gas chromatography. Standalone FIDs can also be used in applications such as landfill gas monitoring, fugitive emissions monitoring and internal combustion engine emissions measurement in stationary or portable instruments.
Agilent 7890 gas chromatograph
Agilent 7890 gas chromatograph
PI Supplied Instrument Name: Agilent 7890 gas chromatograph PI Supplied Instrument Description:Analyzed samples Instrument Name: Gas Chromatograph Instrument Short Name:Gas Chromatograph Instrument Description: Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB02/
5975C mass spectrometer
5975C mass spectrometer
PI Supplied Instrument Name: 5975C mass spectrometer PI Supplied Instrument Description:Analyzed samples Instrument Name: Mass Spectrometer Instrument Short Name:Mass Spec Instrument Description: General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Core
Core
PI Supplied Instrument Name: Core PI Supplied Instrument Description:Used to collect core samples Instrument Name: Push Corer Instrument Short Name: Instrument Description: Capable of being performed in numerous environments, push coring is just as it sounds. Push coring is simply pushing the core barrel (often an aluminum or polycarbonate tube) into the sediment by hand. A push core is useful in that it causes very little disturbance to the more delicate upper layers of a sub-aqueous sediment.
Description obtained from: http://web.whoi.edu/coastal-group/about/how-we-work/field-methods/coring/
Deployment: Spivak_2012
Spivak_2012
shoreside Massachusetts
shoreside
shoreside Massachusetts
shoreside