Grazing experiment 6: Cellular lipid data for low-high pCO2 acclimated Rhodomonas sp. cultures, 2011-2016 (E Hux Response to pCO2 project)

Website: https://www.bco-dmo.org/dataset/669863
Data Type: experimental
Version:
Version Date: 2016-12-12

Project
» Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton (E Hux Response to pCO2)
ContributorsAffiliationRole
Olson, Brady M.Western Washington University (WWU)Principal Investigator
Love, BrookeWestern Washington University (WWU)Co-Principal Investigator
Strom, SuzanneWestern Washington University (WWU)Co-Principal Investigator
Still, Kelly AnnWestern Washington University (WWU)Student
Copley, NancyWoods Hole Oceanographic Institution (WHOI BCO-DMO)BCO-DMO Data Manager


Dataset Description

Related Reference: 
Still, Kelly Ann, Microzooplankton grazing, growth and gross growth efficiency are affected by pCO2 induced changes in phytoplankton biology. (Masters Thesis) Western Washington University. http://cedar.wwu.edu/cgi/viewcontent.cgi?article=1490&context=wwuet


Methods & Sampling

The phytoplankton Rhodomonas sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv).  Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients.  Some of the culture removed was used to evaluate chemical parameters.  Samples for lipid mass were collected on day 16.  Samples were filtered onto muffled glass fiber filters, wrapped in muffled foil and flash frozen on dry ice.  Samples were then stored at    -80° C until analysis.  Filters were transferred to a tissue grinder containing 1.9 ml of 1:2 v/v CHCl3: MeOH and 0.125 ml deionized water.  Filters were homogenized with the solution and transferred to a clean glass culture tube and vortexed for 1 minute.  Samples were then sonicated in a water bath at room temperature for 10 minutes and then centrifuged at 2000 rpm for 10 minutes.  The supernatant was then transferred to another glass culture tube and 0.625 ml of CHCl3 and 0.625 ml deionized water were added and then centrifuged another 10 minutes.  The samples were now separated into two phases and the bottom organic phase was collected using a Pasteur pipet and transferred to a pre-weighed glass culture tube.  Samples were evaporated with nitrogen gas to dryness and weighed to determine total cellular lipid mass.


Data Processing Description

Data are raw picogram lipid per cell.

BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions


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Data Files

File
expt6_lipid.csv
(Comma Separated Values (.csv), 804 bytes)
MD5:2d5669d87f3571626dc349ba63e8f8d5
Primary data file for dataset ID 669863

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Parameters

ParameterDescriptionUnits
treatment_replicateSample identifier that names the treatment replicate and the day of semi-continuous culture unitless
pre_weightweight of sample tube milligrams (mg)
weight_totalweight of dried lipid sample plus the tube milligrams (mg)
weight_lipidtotal lipid weight milligrams (mg)
vol_filtvolume of sample filtered milliliters (ml)
cells_filtered_mlconcentration of cells on filter milliliters (ml)
cells_filtered_totalthe number of cells on the filter cells
lipids_mg_cellweight of lipids per cell milligram/cell (mg/cell)
lipids_pg_cellweight of lipids per cell picograms/cell (pg/cell)


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Instruments

Dataset-specific Instrument Name
Spec20D+ spectrophotometer
Generic Instrument Name
Spectrophotometer
Generic Instrument Description
An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples.

Dataset-specific Instrument Name
Generic Instrument Name
scale
Dataset-specific Description
Used to weigh extracted lipids
Generic Instrument Description
An instrument used to measure weight or mass.


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Deployments

Lab_Olson_B

Website
Platform
WWU
Start Date
2011-03-31
End Date
2016-09-15
Description
laboratory experiments


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Project Information

Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton (E Hux Response to pCO2)


Description from NSF award abstract:
The calcifying Haptophyte Emiliania huxleyi appears to be acutely sensitive to the rising concentration of ocean pCO2. Documented responses by E. huxleyi to elevated pCO2 include modifications to their calcification rate and cell size, malformation of coccoliths, elevated growth rates, increased organic carbon production, lowering of PIC:POC ratios, and elevated production of the active climate gas DMS. Changes in these parameters are mechanisms known to elicit alterations in grazing behavior by microzooplankton, the oceans dominant grazer functional group. The investigators hypothesize that modifications to the physiology and biochemistry of calcifying and non-calcifying Haptophyte Emiliania huxleyi in response to elevated pCO2 will precipitate alterations in microzooplankton grazing dynamics. To test this hypothesis, they will conduct controlled laboratory experiments where several strains of E. huxleyi are grown at several CO2 concentrations. After careful characterization of the biochemical and physiological responses of the E. huxleyi strains to elevated pCO2, they will provide these strains as food to several ecologically-important microzooplankton and document grazing dynamics. E. huxleyi is an ideal organism for the study of phytoplankton and microzooplankton responses to rising anthropogenic CO2, the effects of which in the marine environment are called ocean acidification; E. huxleyi is biogeochemically important, is well studied, numerous strains are in culture that exhibit variation in the parameters described above, and they are readily fed upon by ecologically important microzooplankton.

The implications of changes in microzooplankton grazing for carbon cycling, specifically CaCO3 export, DMS production, nutrient regeneration in surface waters, and carbon transfer between trophic levels are profound, as this grazing, to a large degree, regulates all these processes. E. huxleyi is a model prey organism because it is one of the most biogeochemically influential global phytoplankton. It forms massive seasonal blooms, contributes significantly to marine inorganic and organic carbon cycles, is a large producer of the climatically active gas DMS, and is a source of organic matter for trophic levels both above and below itself. The planned controlled study will increase our knowledge of the mechanisms that drive patterns of change between trophic levels, thus providing a wider array of tools necessary to understand the complex nature of ocean acidification field studies, where competing variables can confound precise interpretation.



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Funding

Funding SourceAward
NSF Division of Ocean Sciences (NSF OCE)

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