1b:I[42426,["/_next/static/immutable/chunks/1xctgzqre-wsy.js","/_next/static/immutable/chunks/06_-wz6pdtp2b.js","/_next/static/immutable/chunks/30e52g49hg40p.js","/_next/static/immutable/chunks/02yq6tul2z-oh.js","/_next/static/immutable/chunks/10bx_ec2vj7n_.js","/_next/static/immutable/chunks/33bfn03fo8xnt.js","/_next/static/immutable/chunks/0tfjf78scu61b.js","/_next/static/immutable/chunks/31xnvej4n1bi9.js"],"default"] 1c:T6e1,

The phytoplankton Rhodomonas sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv). Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients. Some of the culture removed was used to evaluate chemical parameters.

pCO2 Rhodomonas: Samples for total alkalinity were taken on growth days 1, 3, 5, 7, 9, 11, 12, 14 and 17 and preserved with HgCl2 and stored at 4° until analysis. Alkalinity was measured by gran titration using a Titrando 888, and 0.1 N HCl titrant, in a temperature controlled titration vessel. DIC samples were filtered through a 0.2 µm nylon syringe filter on the morning of the experimental day, then stored in airtight vials at 4°C until analysis within 60 days using an Apollo SciTech DIC Analyzer AS-C3 which incorporates the LI-7000 CO2/H2O Analyzer. Other parameters were calculated with CO2sys.

pCO2 Rhodomonas and long-term grazing by Gyrodinium: Samples for total alkalinity of pre-equilibrated media were taken on growth day 11 and from the Rhodomonas plus Gyrodinium grazing treatments on day16 and preserved with HgCl2 and stored at 4° until analysis. Alkalinity was measured by gran titration using a Titrando 888, and 0.1 N HCl titrant, in a temperature controlled titration vessel. DIC samples from the long-term grazing experiment were filtered through a 0.2 µm nylon syringe filter on the morning of the experimental day, then stored in airtight vials at 4°C until analysis within 60 days using an Apollo SciTech DIC Analyzer AS-C3 which incorporates the LI-7000 CO2/H2O Analyzer. Other parameters were calculated with CO2sys.

1d:Ta3e,Description from NSF award abstract: The calcifying Haptophyte Emiliania huxleyi appears to be acutely sensitive to the rising concentration of ocean pCO2. Documented responses by E. huxleyi to elevated pCO2 include modifications to their calcification rate and cell size, malformation of coccoliths, elevated growth rates, increased organic carbon production, lowering of PIC:POC ratios, and elevated production of the active climate gas DMS. Changes in these parameters are mechanisms known to elicit alterations in grazing behavior by microzooplankton, the oceans dominant grazer functional group. The investigators hypothesize that modifications to the physiology and biochemistry of calcifying and non-calcifying Haptophyte Emiliania huxleyi in response to elevated pCO2 will precipitate alterations in microzooplankton grazing dynamics. To test this hypothesis, they will conduct controlled laboratory experiments where several strains of E. huxleyi are grown at several CO2 concentrations. After careful characterization of the biochemical and physiological responses of the E. huxleyi strains to elevated pCO2, they will provide these strains as food to several ecologically-important microzooplankton and document grazing dynamics. E. huxleyi is an ideal organism for the study of phytoplankton and microzooplankton responses to rising anthropogenic CO2, the effects of which in the marine environment are called ocean acidification; E. huxleyi is biogeochemically important, is well studied, numerous strains are in culture that exhibit variation in the parameters described above, and they are readily fed upon by ecologically important microzooplankton. The implications of changes in microzooplankton grazing for carbon cycling, specifically CaCO3 export, DMS production, nutrient regeneration in surface waters, and carbon transfer between trophic levels are profound, as this grazing, to a large degree, regulates all these processes. E. huxleyi is a model prey organism because it is one of the most biogeochemically influential global phytoplankton. It forms massive seasonal blooms, contributes significantly to marine inorganic and organic carbon cycles, is a large producer of the climatically active gas DMS, and is a source of organic matter for trophic levels both above and below itself. The planned controlled study will increase our knowledge of the mechanisms that drive patterns of change between trophic levels, thus providing a wider array of tools necessary to understand the complex nature of ocean acidification field studies, where competing variables can confound precise interpretation.6:["$","$L1b",null,{"dataset":{"_id":"670112","description":"

Related Reference:
\nStill, Kelly Ann, Microzooplankton grazing, growth and gross growth efficiency are affected by pCO₂ induced changes in phytoplankton biology. (Masters Thesis) Western Washington University. http://cedar.wwu.edu/cgi/viewcontent.cgi?article=1490&context=wwuet

\n","problem":"","acquisition":"$1c","processing":"

Data are unprocessed.

BCO-DMO Processing Notes:
\n- added conventional header with dataset name, PI name, version date
\n- modified parameter names to conform with BCO-DMO naming conventions
\n- nd (no data) was entered into all blank cells
\n- replaced spaces with underscores

\n","bcodmoProcessing":"","title":"Grazing experiment 7: Carbonate chemistry data for low-high pCO2 acclimated Rhodomonas sp. cultures and long term grazing treatments (E Hux Response to pCO2 project)","nickname":"Grazing experiment 7: pCO2 with and without grazing by Gyrodinium","dataUrl":"","currentState":"Final no updates expected","currentStateId":"7","validated":true,"restricted":false,"version":"","version_date":"2016-12-14T05:00:00.000Z","abstract":"","citation":"Olson, M. Brady, Love, B., Strom, S. (2016) Grazing experiment 7: Carbonate chemistry data for low-high pCO2 acclimated Rhodomonas sp. cultures and long term grazing treatments (E Hux Response to pCO2 project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2016-12-14 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/670112 [access date]","citationParts":{"identifier":"http://lod.bco-dmo.org/id/dataset/670112","versionDate":"2016-12-14","versionId":"","year":"2016","title":"Grazing experiment 7: Carbonate chemistry data for low-high pCO2 acclimated Rhodomonas sp. cultures and long term grazing treatments (E Hux Response to pCO2 project)","authors":"Olson, M. Brady, Love, B., Strom, S.","publisher":"Biological and Chemical Oceanography Data Management Office (BCO-DMO)."},"doi":"","release_date":"2017-12-31T05:00:00.000Z","field_dataset_location":"","temporal_extent":{"start":null,"end":null},"temporal":{"resolution":"","type":"","start":{"value":"","label":""},"end":{"value":"","label":""}},"spatial_extent":{"shape":"","center":{"latitude":0,"longitude":0},"wkt":"","north":0,"east":0,"south":0,"west":0},"dataset_type":["experimental"],"keywords":["biota","oceans"],"data_files":[{"id":"901356","title":"expt7_pCO2.csv","url":"https://datadocs.bco-dmo.org/dataset/670112/file/3YY3g1PsREY10G/expt7_pCO2.csv","showTableUrl":"https://www.bco-dmo.org/dataset/670112/data/view/901356","description":"Primary data file for dataset ID 670112","filename":"expt7_pCO2.csv","bytesize":12842,"checksum":{"value":"690f0d784e5b5b092a72f9a9fbe42d67","Algorithm":{"_id":"190","name":"MD5"}},"mimetype":"text/csv","fileType":{"_id":"192","name":"Comma Separated Values (.csv)"},"isErddap":"","date":"","isPublicDuringEmbargo":false,"primaryDataFile":true,"showTable":true,"restricted":false}],"supplemental_files":[],"roles":[{"role":{"_id":"138","name":"Principal Investigator"},"performedBy":{"_id":"51528","name":"M. Brady Olson"},"performedAtOrganization":{"_id":"50","name":"Western Washington University"}},{"role":{"_id":"140","name":"Co-Principal Investigator"},"performedBy":{"_id":"51527","name":"Brooke Love"},"performedAtOrganization":{"_id":"50","name":"Western Washington University"}},{"role":{"_id":"140","name":"Co-Principal Investigator"},"performedBy":{"_id":"50471","name":"Suzanne Strom"},"performedAtOrganization":{"_id":"50","name":"Western Washington University"}},{"role":{"_id":"128","name":"Student"},"performedBy":{"_id":"669786","name":"Kelly Ann Still"},"performedAtOrganization":{"_id":"50","name":"Western Washington University"}},{"role":{"_id":"145","name":"BCO-DMO Data Manager"},"performedBy":{"_id":"50396","name":"Nancy Copley"},"performedAtOrganization":{"_id":"191","name":"Woods Hole Oceanographic Institution"}}],"deployments":[{"_id":"521277","name":"Lab_Olson_B","synonyms":[],"dates":{"start":"2011-03-31T00:00:00.000Z","end":"2016-09-15T00:00:00.000Z"},"platform":{"_id":"54249","name":"WWU"},"chiefScientists":[]}],"funding":[{"_id":"517693","name":"OCE-0961229","fundingSource":{"_id":"355","name":"NSF Division of Ocean Sciences"},"url":"https://www.nsf.gov/awardsearch/show-award?AWD_ID=0961229"}],"instruments":[{"_id":"670122","name":"LI-7000 CO2/H2O Analyzer","description":"","type_id":"577","type_name":"LI-COR LI-7000 Gas Analyzer","type_desc":"The LI-7000 gas analyzer is a differential, single source, non-dispersive, infrared gas analyzer. It has two solid state detectors, one each for CO2 and H2O, filters at 4.255 microns and 2.595 microns respectively. CO2 is measured in the range 0-3000ppm, with an accuracy of 1 percent nominally. H2O is measured in the range 0-60 mmol per mol, with an accuracy of one 1 percent.","type_acronym":"LI-COR LI-7000"},{"_id":"670121","name":"","description":"Titrando 888","type_id":"682","type_name":"Titrator","type_desc":"Titrators are instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached.","type_acronym":"Titrator"}],"parameters":[{"_id":"670124","name":"treatment_rep_culture_day","format":"","frictionlessType":"","description":"

Treatment replicate that names the sample and the day of semi-continuous culture

","units":{"_id":"","name":"unitless"},"type":{"_id":"953","name":"replicate","description":"

an identifier used to distinguish between one or more replicate samples

"}},{"_id":"670125","name":"alkalinity","format":"","frictionlessType":"","description":"

total alkalinity of the culture material removed

","units":{"_id":"","name":"micromoles/kilogram (umol/kg)"},"type":{"_id":"1224","name":"TALK","description":"

Total alkalinity

"}},{"_id":"670126","name":"DIC","format":"","frictionlessType":"","description":"

dissolved inorganic carbon

","units":{"_id":"","name":"micromoles/kilogram (umol/kg)"},"type":{"_id":"1187","name":"DIC","description":"

Dissolved Inorganic Carbon

"}},{"_id":"670127","name":"pCO2","format":"","frictionlessType":"","description":"

Partial pressure of carbon dioxide in the water body by computation from pH and alkalinity

","units":{"_id":"","name":"parts per million by volume (ppmv)"},"type":{"_id":"1991","name":"pCO2","description":"

Partial pressure of carbon dioxide {pCO2} in the water body by computation from pH and alkalinity.

"}},{"_id":"670128","name":"description","format":"","frictionlessType":"","description":"

description of the sub-dataset: Rhodomoas: no grazers; Rhodomonas_and_Gyrodinium: grazers present

","units":{"_id":"","name":"unitless"},"type":{"_id":"1741","name":"sample_descrip","description":"

text description or other traits of the sample collected

"}}],"related_publications":[],"related_datasets":[],"services":[{"grouping":"","items":[{"name":"View Data","description":"An in-browser view of the primary tabular data file","url":"https://www.bco-dmo.org/dataset/670112/data/view","contentType":"text/html","actionType":"view"}]},{"grouping":"Metadata","items":[{"name":"HTML","description":"A simple HTML page of the dataset metadata","url":"https://www.bco-dmo.org/dataset/670112/description","contentType":"text/html","actionType":"view"},{"name":"PDF","description":"A PDF of the HTML dataset metadata","url":"https://www.bco-dmo.org/dataset/670112/Dataset_description.pdf","contentType":"application/pdf","actionType":"download"},{"name":"ISO 19115-2 (NOAA Profile)","description":"","url":"https://www.bco-dmo.org/dataset/670112/iso","contentType":"application/xml","actionType":"download"}]}],"programs":[],"projects":[{"_id":"517694","name":"Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton","acronym":"E Hux Response to pCO2","description":"$1d"}],"archives":[],"license":{"name":"Creative Commons Attribution 4.0","id":"CC-BY-4.0","uri":"https://creativecommons.org/licenses/by/4.0/","spdxUri":"https://spdx.org/licenses/CC-BY-4.0"},"metrics":{"views":{"count":38},"downloads":{"count":332}}},"fileId":"901356"}]