http://lod.bco-dmo.org/id/dataset/670162
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2016-12-14
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Grazing experiment 7: Cellular lipid data for low-high pCO2 acclimated Rhodomonas sp. cultures, 2011-2016 (E Hux Response to pCO2 project)
2016-12-14
publication
2016-12-14
revision
BCO-DMO Linked Data URI
2016-12-14
creation
http://lod.bco-dmo.org/id/dataset/670162
M. Brady Olson
Western Washington University
principalInvestigator
Brooke Love
Western Washington University
principalInvestigator
Suzanne Strom
Western Washington University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Olson, M. Brady, Love, B., Strom, S. (2016) Grazing experiment 7: Cellular lipid data for low-high pCO2 acclimated Rhodomonas sp. cultures, 2011-2016 (E Hux Response to pCO2 project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2016-12-14 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/670162 [access date]
Cellular lipid data for low-high pCO2 acclimated Rhodomonas sp. cultures Dataset Description: <p><strong>Related Reference:&nbsp;</strong><br />
Still, Kelly Ann, Microzooplankton grazing, growth and gross growth efficiency are affected by pCO<sub>2</sub> induced changes in phytoplankton biology. (Masters Thesis) Western Washington University.&nbsp;<a href="http://cedar.wwu.edu/cgi/viewcontent.cgi?article=1490&amp;context=wwuet">http://cedar.wwu.edu/cgi/viewcontent.cgi?article=1490&amp;context=wwuet</a></p> Methods and Sampling: <p>The phytoplankton Rhodomonas sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv). Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients. Some of the culture removed was used to evaluate chemical parameters. Samples for lipid mass were collected on day 16. Samples were filtered onto muffled glass fiber filters, wrapped in muffled foil and flash frozen on dry ice. Samples were then stored at -80° C until analysis. Filters were transferred to a tissue grinder containing 1.9 ml of 1:2 v/v CHCl3: MeOH and 0.125 ml deionized water. Filters were homogenized with the solution and transferred to a clean glass culture tube and vortexed for 1 minute. Samples were then sonicated in a water bath at room temperature for 10 minutes and then centrifuged at 2000 rpm for 10 minutes. The supernatant was then transferred to another glass culture tube and 0.625 ml of CHCl3 and 0.625 ml deionized water were added and then centrifuged another 10 minutes. The samples were now separated into two phases and the bottom organic phase was collected using a Pasteur pipet and transferred to a pre-weighed glass culture tube. Samples were evaporated with nitrogen gas to dryness and weighed to determine total cellular lipid mass.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0961229 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0961229
completed
M. Brady Olson
Western Washington University
(360) 650-7400
1900 Shannon Point Rd.
Anacortes
WA
98221
United States
Brady.Olson@wwu.edu
pointOfContact
Brooke Love
Western Washington University
(360) 650-2894
516 High St. Mail Stop 9181
Bellingham
WA
98225
United States
brooke.love@wwu.edu
pointOfContact
Suzanne Strom
Western Washington University
360-293-2188
Shannon Point Marine Center 1900 Shannon Point Rd
Anacortes
WA
98221
USA
suzanne.strom@wwu.edu
pointOfContact
asNeeded
Unknown
treatment_replicate
pre_weight
weight_total
weight_lipid
vol_filt
cells_filtered_ml
cells_filtered_total
lipids_mg_cell
lipids_pg_cell
Spec20D+ spectrophotometer
theme
None, User defined
sample identification
weight
volume of water filtered
cell_concentration
count
featureType
BCO-DMO Standard Parameters
scale or balance
Spectrophotometer
instrument
BCO-DMO Standard Instruments
Lab_Olson_B
service
Deployment Activity
Bellingham, WA
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton
https://osprey.bco-dmo.org/project/517694
Planktonic interactions in a changing ocean: Biological responses of Emiliania huxleyi to elevated pCO2 and their effects on microzooplankton
<p><em><strong>Description from NSF award abstract:</strong></em><br />
The calcifying Haptophyte <em>Emiliania huxleyi </em>appears to be acutely sensitive to the rising concentration of ocean pCO2. Documented responses by <em>E. huxleyi </em>to elevated pCO2 include modifications to their calcification rate and cell size, malformation of coccoliths, elevated growth rates, increased organic carbon production, lowering of PIC:POC ratios, and elevated production of the active climate gas DMS. Changes in these parameters are mechanisms known to elicit alterations in grazing behavior by microzooplankton, the oceans dominant grazer functional group. The investigators hypothesize that modifications to the physiology and biochemistry of calcifying and non-calcifying Haptophyte <em>Emiliania huxleyi </em>in response to elevated pCO2 will precipitate alterations in microzooplankton grazing dynamics. To test this hypothesis, they will conduct controlled laboratory experiments where several strains of <em>E. huxleyi</em> are grown at several CO2 concentrations. After careful characterization of the biochemical and physiological responses of the <em>E. huxleyi</em> strains to elevated pCO2, they will provide these strains as food to several ecologically-important microzooplankton and document grazing dynamics.<em> E. huxleyi</em> is an ideal organism for the study of phytoplankton and microzooplankton responses to rising anthropogenic CO2, the effects of which in the marine environment are called ocean acidification; <em>E. huxleyi </em>is biogeochemically important, is well studied, numerous strains are in culture that exhibit variation in the parameters described above, and they are readily fed upon by ecologically important microzooplankton.</p>
<p>The implications of changes in microzooplankton grazing for carbon cycling, specifically CaCO3 export, DMS production, nutrient regeneration in surface waters, and carbon transfer between trophic levels are profound, as this grazing, to a large degree, regulates all these processes. <em>E. huxleyi </em>is a model prey organism because it is one of the most biogeochemically influential global phytoplankton. It forms massive seasonal blooms, contributes significantly to marine inorganic and organic carbon cycles, is a large producer of the climatically active gas DMS, and is a source of organic matter for trophic levels both above and below itself. The planned controlled study will increase our knowledge of the mechanisms that drive patterns of change between trophic levels, thus providing a wider array of tools necessary to understand the complex nature of ocean acidification field studies, where competing variables can confound precise interpretation.</p>
E Hux Response to pCO2
largerWorkCitation
project
eng; USA
biota
oceans
Bellingham, WA
2016-12-14
0
BCO-DMO catalogue of parameters from Grazing experiment 7: Cellular lipid data for low-high pCO2 acclimated Rhodomonas sp. cultures, 2011-2016 (E Hux Response to pCO2 project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/670174.rdf
Name: treatment_replicate
Units: unitless
Description: <p>Sample identifier that names the treatment replicate and the day of semi-continuous culture</p>
http://lod.bco-dmo.org/id/dataset-parameter/670175.rdf
Name: pre_weight
Units: milligrams (mg)
Description: <p>weight of sample tube</p>
http://lod.bco-dmo.org/id/dataset-parameter/670176.rdf
Name: weight_total
Units: milligrams (mg)
Description: <p>weight of dried lipid sample plus the tube</p>
http://lod.bco-dmo.org/id/dataset-parameter/670177.rdf
Name: weight_lipid
Units: milligrams (mg)
Description: <p>total lipid weight</p>
http://lod.bco-dmo.org/id/dataset-parameter/670178.rdf
Name: vol_filt
Units: milliliters (ml)
Description: <p>volume of sample filtered</p>
http://lod.bco-dmo.org/id/dataset-parameter/670179.rdf
Name: cells_filtered_ml
Units: milliliters (ml)
Description: <p>concentration of cells on filter</p>
http://lod.bco-dmo.org/id/dataset-parameter/670180.rdf
Name: cells_filtered_total
Units: cells
Description: <p>the number of cells on the filter</p>
http://lod.bco-dmo.org/id/dataset-parameter/670181.rdf
Name: lipids_mg_cell
Units: milligram/cell (mg/cell)
Description: <p>weight of lipids per cell</p>
http://lod.bco-dmo.org/id/dataset-parameter/670182.rdf
Name: lipids_pg_cell
Units: picograms/cell (pg/cell)
Description: <p>weight of lipids per cell</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
826
https://datadocs.bco-dmo.org/file/933jorNSNzVDnO/expt7_lipid.csv
expt7_lipid.csv
Primary data file for dataset ID 670162
download
https://osprey.bco-dmo.org/dataset/670162/data/download
download
onLine
dataset
<p>The phytoplankton Rhodomonas sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (750ppmv), and High (1000ppmv). Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients. Some of the culture removed was used to evaluate chemical parameters. Samples for lipid mass were collected on day 16. Samples were filtered onto muffled glass fiber filters, wrapped in muffled foil and flash frozen on dry ice. Samples were then stored at -80° C until analysis. Filters were transferred to a tissue grinder containing 1.9 ml of 1:2 v/v CHCl3: MeOH and 0.125 ml deionized water. Filters were homogenized with the solution and transferred to a clean glass culture tube and vortexed for 1 minute. Samples were then sonicated in a water bath at room temperature for 10 minutes and then centrifuged at 2000 rpm for 10 minutes. The supernatant was then transferred to another glass culture tube and 0.625 ml of CHCl3 and 0.625 ml deionized water were added and then centrifuged another 10 minutes. The samples were now separated into two phases and the bottom organic phase was collected using a Pasteur pipet and transferred to a pre-weighed glass culture tube. Samples were evaporated with nitrogen gas to dryness and weighed to determine total cellular lipid mass.</p>
Specified by the Principal Investigator(s)
<p>Data are raw picogram lipid per cell.</p>
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: PI Supplied Instrument Description:Used to weigh extracted lipids Instrument Name: scale or balance Instrument Short Name: Instrument Description: Devices that determine the mass or weight of a sample. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB13/
Spec20D+ spectrophotometer
Spec20D+ spectrophotometer
PI Supplied Instrument Name: Spec20D+ spectrophotometer Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/
Deployment: Lab_Olson_B
Lab_Olson_B
WWU
laboratory
WWU
laboratory