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        <gco:CharacterString>Bulk raw isotopic data from tissue samples from three species of tunas collected in the eastern tropical Pacific Ocean onboard commercial tuna purse-seine vessels Dataset Description: &amp;lt;p&amp;gt;Bulk raw isotopic data from tissue samples from three species of tunas collected in the eastern tropical Pacific Ocean onboard commercial tuna purse-seine vessels.&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Sampling Methodology: &amp;lt;/strong&amp;gt;Three species of tuna, yellowfin (Ta.; &amp;lt;em&amp;gt;Thunnus albacares&amp;lt;/em&amp;gt;), skipjack (Kp.; &amp;lt;em&amp;gt;Katsuwonus pelamis&amp;lt;/em&amp;gt;), and bigeye (To.; &amp;lt;em&amp;gt;Thunnus obesus&amp;lt;/em&amp;gt;) tunas, were sampled year-round during 2003-2005 by observers of the Inter-American Tropical Tuna Commission onboard purse-seine fishing vessels. Samples of dorsal white muscle were taken from each fish adjacent to the second dorsal fin. Fish of uniform size were used for analysis: skipjack tuna 450-550 mm, yellowfin tuna 500-700 mm, and bigeye tuna 450-550 mm. All samples were stored frozen until further processing in the laboratory.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Analytical Methodology: &amp;lt;/strong&amp;gt;Methods are described in Hetherington et al. (2016). Briefly: Isotopic analysis of bulk muscle tissue of the tunas was performed at the University of Hawaii’s Isotope Biogeochemistry Laboratory. Stable isotope values of nitrogen were determined using an on-line carbon-nitrogen analyzer coupled with an isotope ratio mass spectrometer (FinniganConFlo II/Delta-Plus). Isotope values are reported in conventional delta-notation relative to the international standards, atmospheric N&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; and V-PDB, for N and C, respectively. Mean accuracy of all stable isotopic analyses was &amp;amp;lt; +/-&amp;amp;nbsp;0.1 ‰ (1 sd) based on triplicate analysis of in-house reference materials (glycine standard and tuna muscle) with known δ&amp;lt;sup&amp;gt;15&amp;lt;/sup&amp;gt;N values.&amp;lt;/p&amp;gt;</gco:CharacterString>
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&lt;p&gt;Evidence increasingly demonstrates that selective removal of marine life can induce restructuring of marine food webs. Trophic structure is the central component of mass balance models, widely used tools to evaluate fisheries in an ecosystem context. Food web structure is commonly determined by stomach contents or by bulk tissue stable isotope analyses, both of which are limited in terms of resolution and versatility. The investigators will refine a tool, Amino Acid Compound-Specific Isotopic Analyses (AA-CSIA), which can be broadly applicable for quantifying the time-integrated trophic position (TP) of consumers. Differences in source and trophic nitrogen isotopic composition for specific amino acids will provide an unambiguous and integrated measure of fractional trophic TP across multiple phyla, regardless of an animal's physiological condition or of the biogeochemical cycling at the base of the food web. AA-CSIA will allow testing of the efficacy of trophic position estimates derived from ecosystem-based models and promote the evolution of these models into decision-support tools.&lt;/p&gt;
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&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Analytical Methodology: &amp;lt;/strong&amp;gt;Methods are described in Hetherington et al. (2016). Briefly: Isotopic analysis of bulk muscle tissue of the tunas was performed at the University of Hawaii’s Isotope Biogeochemistry Laboratory. Stable isotope values of nitrogen were determined using an on-line carbon-nitrogen analyzer coupled with an isotope ratio mass spectrometer (FinniganConFlo II/Delta-Plus). Isotope values are reported in conventional delta-notation relative to the international standards, atmospheric N&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; and V-PDB, for N and C, respectively. Mean accuracy of all stable isotopic analyses was &amp;amp;lt; +/-&amp;amp;nbsp;0.1 ‰ (1 sd) based on triplicate analysis of in-house reference materials (glycine standard and tuna muscle) with known δ&amp;lt;sup&amp;gt;15&amp;lt;/sup&amp;gt;N values.&amp;lt;/p&amp;gt;</gco:CharacterString>
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              <gmd:MD_ProgressCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MD_ProgressCode" codeListValue="completed"/>
            </gmi:status>
            <gmi:type>
              <gmi:MI_OperationTypeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MI_OperationTypeCode" codeListValue="real"/>
            </gmi:type>
            <gmi:parentOperation gco:nilReason="inapplicable"/>
            <gmi:platform>
  <gmi:MI_Platform>
    <gmi:identifier>
      <gmd:MD_Identifier>
        <gmd:code>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/54110.rdf"
           xlink:actuate="onRequest">Fishing Vessels</gmx:Anchor>
        </gmd:code>
      </gmd:MD_Identifier>
    </gmi:identifier>
    <gmi:description>
      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/54110.rdf" xlink:title="Fishing Vessels" xlink:actuate="onRequest">vessel</gmx:Anchor>
    </gmi:description>
    <gmi:instrument gco:nilReason="unknown"/>
  </gmi:MI_Platform>
</gmi:platform>
            </gmi:MI_Operation>
      </gmi:operation><gmi:platform>
  <gmi:MI_Platform>
    <gmi:identifier>
      <gmd:MD_Identifier>
        <gmd:code>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/54110.rdf"
           xlink:actuate="onRequest">Fishing Vessels</gmx:Anchor>
        </gmd:code>
      </gmd:MD_Identifier>
    </gmi:identifier>
    <gmi:description>
      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/54110.rdf" xlink:title="Fishing Vessels" xlink:actuate="onRequest">vessel</gmx:Anchor>
    </gmi:description>
    <gmi:instrument gco:nilReason="unknown"/>
  </gmi:MI_Platform>
</gmi:platform>
          </gmi:MI_AcquisitionInformation>
  </gmi:acquisitionInformation>
</gmi:MI_Metadata>
