http://lod.bco-dmo.org/id/dataset/675199
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2017-01-18
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Carbonate chemistry associated with copepod experiments with varying CO2 (OA-Copepod_PreyQual project)
2017-01-18
publication
2017-01-18
revision
BCO-DMO Linked Data URI
2017-01-18
creation
http://lod.bco-dmo.org/id/dataset/675199
M. Brady Olson
Western Washington University
principalInvestigator
Julie E. Keister
University of Washington
principalInvestigator
Brooke Love
Western Washington University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Olson, M. Brady, Love, B., Keister, J. E. (2017) Carbonate chemistry associated with copepod experiments with varying CO2 (OA-Copepod_PreyQual project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2017-01-18 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/675199 [access date]
Carbonate chemistry for copepod experiments Dataset Description: <p>This dataset includes carbonate chemistry data from copepod grazing and egg production experiments with varying pCO2 levels, from 2014 and 2015 studies.</p> Methods and Sampling: <p>DIC samples were very gently filtered through a GFF syringe filter on the morning of the experimental day, then stored in air tight vials which were overflowed to minimize air exposure and allowed to come to room temperature until analysis on the same day using an Apollo SciTech DIC Analyzer AS-C3 which incorporates the LI-7000 CO2/H2O Analyzer, then corrected for density based on temperature and salinity. &nbsp;Samples for pH were collected in a similar fashion to DIC, and also run on the same day after reaching 25 degrees in a temperature controlled water bath. &nbsp;Samples were carefully transferred from the vials to a jacketed 5 cm cuvette.&nbsp; pH was determined via spectrophotometric methods, with the addition of 20 uL of m-cresol blue to each sample after taking a baseline measurement.&nbsp; The ratio of the two absorbance peaks was corrected based on samples in which two dye additions were made. &nbsp;Other parameters were calculated with CO2sys.&nbsp; Some samples for total alkalinity were also run for QC purposes.&nbsp; These were preserved with HgCl2 and stored in 120 ml glass bottles with Teflon lined screw cap seals.&nbsp; Alkalinity was measured by gran titration using a Titrando 888, and 0.1 N HCl titrant, in a temperature controlled titration vessel.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1220664 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1220664
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1220381 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1220381
completed
M. Brady Olson
Western Washington University
(360) 650-7400
1900 Shannon Point Rd.
Anacortes
WA
98221
United States
Brady.Olson@wwu.edu
pointOfContact
Julie E. Keister
University of Washington
206-543-7620
School of Oceanography Box 357940
Seattle
WA
98195
United States
jkeister@uw.edu
pointOfContact
Brooke Love
Western Washington University
(360) 650-2894
516 High St. Mail Stop 9181
Bellingham
WA
98225
United States
brooke.love@wwu.edu
pointOfContact
asNeeded
Unknown
year
expt
sample
date
experiment
sample_type
treatment
temp
DIC
TA
pH
pCO2
Apollo SciTech DIC Analyzer AS-C3 with LI-7000 CO2/H2O Analyzer
Titrando 888
theme
None, User defined
year
experiment id
sample identification
date
experiment type
sample type
treatment
water temperature at measurement depth
dissolved inorganic Carbon
total alkalinity (TA)
pH
Partial pressure of CO2
featureType
BCO-DMO Standard Parameters
CO2 Analyzer
Spectrophotometer
Titrator
instrument
BCO-DMO Standard Instruments
Lab_Olson_B
service
Deployment Activity
Bellingham, WA
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=503477
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
NSF Climate Research Investment (CRI) activities that were initiated in 2010 are now included under Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES). SEES is a portfolio of activities that highlights NSF's unique role in helping society address the challenge(s) of achieving sustainability. Detailed information about the SEES program is available from NSF (https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=504707).
In recognition of the need for basic research concerning the nature, extent and impact of ocean acidification on oceanic environments in the past, present and future, the goal of the SEES: OA program is to understand (a) the chemistry and physical chemistry of ocean acidification; (b) how ocean acidification interacts with processes at the organismal level; and (c) how the earth system history informs our understanding of the effects of ocean acidification on the present day and future ocean.
Solicitations issued under this program:
NSF 10-530, FY 2010-FY2011
NSF 12-500, FY 2012
NSF 12-600, FY 2013
NSF 13-586, FY 2014
NSF 13-586 was the final solicitation that will be released for this program.
PI Meetings:
1st U.S. Ocean Acidification PI Meeting(March 22-24, 2011, Woods Hole, MA)
2nd U.S. Ocean Acidification PI Meeting(Sept. 18-20, 2013, Washington, DC)
3rd U.S. Ocean Acidification PI Meeting (June 9-11, 2015, Woods Hole, MA – Tentative)
NSF media releases for the Ocean Acidification Program:
Press Release 10-186 NSF Awards Grants to Study Effects of Ocean Acidification
Discovery Blue Mussels "Hang On" Along Rocky Shores: For How Long?
Discovery nsf.gov - National Science Foundation (NSF) Discoveries - Trouble in Paradise: Ocean Acidification This Way Comes - US National Science Foundation (NSF)
Press Release 12-179 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: Finding New Answers Through National Science Foundation Research Grants - US National Science Foundation (NSF)
Press Release 13-102 World Oceans Month Brings Mixed News for Oysters
Press Release 13-108 nsf.gov - National Science Foundation (NSF) News - Natural Underwater Springs Show How Coral Reefs Respond to Ocean Acidification - US National Science Foundation (NSF)
Press Release 13-148 Ocean acidification: Making new discoveries through National Science Foundation research grants
Press Release 13-148 - Video nsf.gov - News - Video - NSF Ocean Sciences Division Director David Conover answers questions about ocean acidification. - US National Science Foundation (NSF)
Press Release 14-010 nsf.gov - National Science Foundation (NSF) News - Palau's coral reefs surprisingly resistant to ocean acidification - US National Science Foundation (NSF)
Press Release 14-116 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: NSF awards $11.4 million in new grants to study effects on marine ecosystems - US National Science Foundation (NSF)
SEES-OA
largerWorkCitation
program
Impacts on copepod populations mediated by changes in prey quality
https://osprey.bco-dmo.org/project/2218
Impacts on copepod populations mediated by changes in prey quality
<p>Research shows that ocean acidification (OA) has physiological consequences for individual organisms, even those lacking calcium carbonate skeletal structures. However, this existing research does not adequately address how OA effects to individuals are linked across trophic levels. Pelagic copepods are critical players in most marine biogeochemical cycles. Their consumption of phytoplankton and microzooplankton is the primary mechanism by which bacterial and phytoplankton production is transferred to higher trophic levels. Despite their high abundance and ecological importance, copepods have received little research attention concerning OA. The few extant studies focused on direct acute effects to copepods (e.g. egg hatching, survival) under elevated pCO2, and few significant effects have been observed at predicted future pCO2. However, there is increasing recognition that OA significantly affects their phytoplankton prey, including elevating growth rates, increasing cell sizes, altering nutrient uptake and ratios, and chemical composition. Because copepod grazing, egg production, and hatching success all can vary with these prey characteristics, OA mediated changes in phytoplankton quality may be an important indirect mechanism through which OA acts on copepod populations and, ultimately, marine food webs.</p>
<p>This study that will advance our understanding of how copepod populations may be affected by OA, specifically through OA induced changes in phytoplankton quality. Our core objective is to determine how changes in phytoplankton physiology and biochemistry (e.g. lipid composition) affect copepod egg production, hatching, and ontogenetic development of nauplii. We will also include a subset of experiments to test whether OA affects copepod reproductive output independent of changes to prey. To achieve these research goals, the diatom, <em>Ditylum brightwellii</em>, and dinoflagellate, <em>Prorocentrum micans</em>, will be cultured semi-continuously under several pCO2 concentrations, during which time we will characterize changes in their physiology and biochemistry. The copepods, <em>Calanus pacificus</em>, a large, high lipid-bearing marine species, and <em>Acartia clausi</em>, a smaller, low lipid-bearing estuarine species, will be maintained across varying pCO2 concentrations and fed these pCO2-acclimated prey, and their grazing and reproductive capability quantified. The copepods and phytoplankton used in this study will be collected from the Salish Sea, a region already experiencing periods of high pCO2/H+ (>1000 ppm, pH 7.5) on varying timescales. Therefore, this research addresses a question of how future climate change may impact marine ecosystems, but also is relevant to pCO2/H+ variability presently experienced in coastal environments.</p>
OA-Copepod_PreyQual
largerWorkCitation
project
eng; USA
oceans
Bellingham, WA
2017-01-18
Puget Sound, Salish Sea
0
BCO-DMO catalogue of parameters from Carbonate chemistry associated with copepod experiments with varying CO2 (OA-Copepod_PreyQual project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/675217.rdf
Name: year
Units: unitless
Description: <p>experiment year</p>
http://lod.bco-dmo.org/id/dataset-parameter/675218.rdf
Name: expt
Units: unitless
Description: <p>experiment number</p>
http://lod.bco-dmo.org/id/dataset-parameter/675219.rdf
Name: sample
Units: unitless
Description: <p>sample code used in experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/675220.rdf
Name: date
Units: unitless
Description: <p>date sample was taken</p>
http://lod.bco-dmo.org/id/dataset-parameter/675221.rdf
Name: experiment
Units: unitless
Description: <p>copepod egg production experiment for the associated chemical sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/675222.rdf
Name: sample_type
Units: unitless
Description: <p>chemical sample from: Algae for feeding copepods; Female jars in CO2 chambers; Blank jars with no copepods in CO2 chambers; Tanks used for storing equilibrated media; Development jars with copepod eggs; or Grazing bottles with copepods having algae grazing measured.</p>
http://lod.bco-dmo.org/id/dataset-parameter/675223.rdf
Name: treatment
Units: unitless
Description: <p>The CO2 treatment for the given sample type.</p>
http://lod.bco-dmo.org/id/dataset-parameter/675224.rdf
Name: temp
Units: degrees Celsius
Description: <p>Temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/675225.rdf
Name: DIC
Units: micromoles/kilogram (umol/kg)
Description: <p>Dissolved inorganic carbon</p>
http://lod.bco-dmo.org/id/dataset-parameter/675226.rdf
Name: TA
Units: equivalents/kilogram(ueq/kg)
Description: <p>Total alkalinity</p>
http://lod.bco-dmo.org/id/dataset-parameter/675227.rdf
Name: pH
Units: unitless
Description: <p>pH of sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/675228.rdf
Name: pCO2
Units: microatmospheres (uatm)
Description: <p>measured pCO2 of sample</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
67496
https://datadocs.bco-dmo.org/file/EKKyEl0hWNMOxG/carb_chem.csv
carb_chem.csv
Primary data file for dataset ID 675199
download
https://osprey.bco-dmo.org/dataset/675199/data/download
download
onLine
dataset
<p>DIC samples were very gently filtered through a GFF syringe filter on the morning of the experimental day, then stored in air tight vials which were overflowed to minimize air exposure and allowed to come to room temperature until analysis on the same day using an Apollo SciTech DIC Analyzer AS-C3 which incorporates the LI-7000 CO2/H2O Analyzer, then corrected for density based on temperature and salinity. &nbsp;Samples for pH were collected in a similar fashion to DIC, and also run on the same day after reaching 25 degrees in a temperature controlled water bath. &nbsp;Samples were carefully transferred from the vials to a jacketed 5 cm cuvette.&nbsp; pH was determined via spectrophotometric methods, with the addition of 20 uL of m-cresol blue to each sample after taking a baseline measurement.&nbsp; The ratio of the two absorbance peaks was corrected based on samples in which two dye additions were made. &nbsp;Other parameters were calculated with CO2sys.&nbsp; Some samples for total alkalinity were also run for QC purposes.&nbsp; These were preserved with HgCl2 and stored in 120 ml glass bottles with Teflon lined screw cap seals.&nbsp; Alkalinity was measured by gran titration using a Titrando 888, and 0.1 N HCl titrant, in a temperature controlled titration vessel.</p>
Specified by the Principal Investigator(s)
<p>Unprocessed raw data.</p>
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- combined data for all 2014 and 2015 grazing experiments<br />
- added columns for year and experiment<br />
- replaced spaces with underscore&nbsp;</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Apollo SciTech DIC Analyzer AS-C3 with LI-7000 CO2/H2O Analyzer
Apollo SciTech DIC Analyzer AS-C3 with LI-7000 CO2/H2O Analyzer
PI Supplied Instrument Name: Apollo SciTech DIC Analyzer AS-C3 with LI-7000 CO2/H2O Analyzer PI Supplied Instrument Description:To measure dissolved inorganic carbon (DIC) Instrument Name: CO2 Analyzer Instrument Short Name:CO2 Analyzer Instrument Description: Measures atmospheric carbon dioxide (CO2) concentration. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/382/
PI Supplied Instrument Name: PI Supplied Instrument Description:To measure pH Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/
Titrando 888
Titrando 888
PI Supplied Instrument Name: Titrando 888 PI Supplied Instrument Description:To measure alkalinity Instrument Name: Titrator Instrument Short Name:Titrator Instrument Description: Titrators are instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB12/
Deployment: Lab_Olson_B
Lab_Olson_B
WWU
laboratory
WWU
laboratory