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            <gco:CharacterString>Cite this dataset as: Olson, R., Popp, B., Drazen, J. (2017) Bulk raw isotopic data from tissue samples from non-tuna species collected in the eastern tropical Pacific Ocean onboard two NOAA research ships, David Starr Jordan and McArthur II, (cruises SWFSC1630 and SWFSC1631) in 2006. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2017-01-18 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.675211.1 [access date]</gco:CharacterString>
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        <gco:CharacterString>Bulk raw isotopic data from tissue samples from non-tuna species collected in the eastern tropical Pacific Ocean onboard two NOAA research ships Dataset Description: &amp;lt;p&amp;gt;Bulk raw isotopic data from tissue samples from two species of ommastrephid cephalopods (squids), two species of mesopelagic myctophid fishes, and two species of euphausiid crustaceans collected in the eastern tropical Pacific Ocean onboard two NOAA research ships.&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Sampling Methodology:&amp;lt;/strong&amp;gt;&amp;amp;nbsp;Zooplankton, small mesopelagic fishes, and squids were collected from July 28 to December 8, 2006 during the National Oceanic and Atmospheric Administration’s (NOAA's) &amp;lt;em&amp;gt;Stenella&amp;lt;/em&amp;gt; Abundance Research (STAR) surveys (Gerrodette et al. 2008). We defined our study area to include a subset of sample locations from the STAR surveys based on the presence of both east-west and north-south productivity gradients across the region, with greater surface chlorophyll &amp;lt;em&amp;gt;a&amp;lt;/em&amp;gt; concentrations at the eastern end of the study area and along the equator, according to published oceanographic data. Zooplankton samples were collected with a cylindrical-conical bongo net (333 um mesh), fished to 200 m approximately two hours after sunset, and the samples were frozen within one hour of collection. Specimens of euphausiid crustaceans, &amp;lt;em&amp;gt;Euphausia distinguenda&amp;lt;/em&amp;gt; (Ed) and &amp;lt;em&amp;gt;E. tenera &amp;lt;/em&amp;gt;(Et) were sorted from the thawed zooplankton samples in the laboratory. Specimens of mesopelagic myctophid fishes &amp;lt;em&amp;gt;Myctophum nitidulum&amp;lt;/em&amp;gt; (Mn) and &amp;lt;em&amp;gt;Symbolophorus reversus&amp;lt;/em&amp;gt; (Sr) were collected by dipnet at night. Specimens of the squids &amp;lt;em&amp;gt;Dosidicus gigas&amp;lt;/em&amp;gt; (Dg) and &amp;lt;em&amp;gt;Sthenoteuthis oualaniensis&amp;lt;/em&amp;gt; (So) also were collected at night, using handlines and jigs. (See Olson et al. 2010, Philbrick et al. 2001 for detailed methods).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Analytical Methodology:&amp;lt;/strong&amp;gt;&amp;amp;nbsp;Methods are described in Hetherington et al. (2016). Briefly: Isotopic analysis of bulk muscle tissue or whole animals was performed at the University of Hawaii’s Isotope Biogeochemistry Laboratory. Stable isotope values of nitrogen were determined using an on-line carbon-nitrogen analyzer coupled with an isotope ratio mass spectrometer (FinniganConFlo II/Delta-Plus). Isotope values are reported in conventional delta-notation relative to the international standards atmospheric N&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; and V-PDB, for N and C, respectively. Mean accuracy of all stable isotopic analyses was &amp;amp;lt; +/-&amp;amp;nbsp;0.1 ‰ (1 sd) based on triplicate analysis of in-house reference materials (glycine standard and tuna muscle) with known δ&amp;lt;sup&amp;gt;15&amp;lt;/sup&amp;gt;N values.&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/616068.rdf" xlink:title="OCE-1040810" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1040810 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1040810</gmx:Anchor>
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To guide program priorities, a Science Steering Committee was formed through Dr. Linda Deegan and the initial Scientific Planning Office at the Marine Biological Laboratory in Woods Hole, MA. This Committee was designed to provide scientific advice and broad direction to NOAA and NSF regarding the CAMEO program.
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&lt;p&gt;Evidence increasingly demonstrates that selective removal of marine life can induce restructuring of marine food webs. Trophic structure is the central component of mass balance models, widely used tools to evaluate fisheries in an ecosystem context. Food web structure is commonly determined by stomach contents or by bulk tissue stable isotope analyses, both of which are limited in terms of resolution and versatility. The investigators will refine a tool, Amino Acid Compound-Specific Isotopic Analyses (AA-CSIA), which can be broadly applicable for quantifying the time-integrated trophic position (TP) of consumers. Differences in source and trophic nitrogen isotopic composition for specific amino acids will provide an unambiguous and integrated measure of fractional trophic TP across multiple phyla, regardless of an animal's physiological condition or of the biogeochemical cycling at the base of the food web. AA-CSIA will allow testing of the efficacy of trophic position estimates derived from ecosystem-based models and promote the evolution of these models into decision-support tools.&lt;/p&gt;
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&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Analytical Methodology:&amp;lt;/strong&amp;gt;&amp;amp;nbsp;Methods are described in Hetherington et al. (2016). Briefly: Isotopic analysis of bulk muscle tissue or whole animals was performed at the University of Hawaii’s Isotope Biogeochemistry Laboratory. Stable isotope values of nitrogen were determined using an on-line carbon-nitrogen analyzer coupled with an isotope ratio mass spectrometer (FinniganConFlo II/Delta-Plus). Isotope values are reported in conventional delta-notation relative to the international standards atmospheric N&amp;lt;sub&amp;gt;2&amp;lt;/sub&amp;gt; and V-PDB, for N and C, respectively. Mean accuracy of all stable isotopic analyses was &amp;amp;lt; +/-&amp;amp;nbsp;0.1 ‰ (1 sd) based on triplicate analysis of in-house reference materials (glycine standard and tuna muscle) with known δ&amp;lt;sup&amp;gt;15&amp;lt;/sup&amp;gt;N values.&amp;lt;/p&amp;gt;</gco:CharacterString>
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