http://lod.bco-dmo.org/id/dataset/675242
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2017-01-18
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Carbon and nitrogen content of Rhodomonas cells grown with varying CO2 levels for copepod experiments, 2015 (OA-Copepod_PreyQual project)
2017-01-18
publication
2017-01-18
revision
BCO-DMO Linked Data URI
2017-01-18
creation
http://lod.bco-dmo.org/id/dataset/675242
M. Brady Olson
Western Washington University
principalInvestigator
Julie E. Keister
University of Washington
principalInvestigator
Brooke Love
Western Washington University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Olson, M. Brady, Love, B., Keister, J. E. (2017) Carbon and nitrogen content of Rhodomonas cells grown with varying CO2 levels for copepod experiments, 2015 (OA-Copepod_PreyQual project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2017-01-18 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/675242 [access date]
Rhodomonas carbon and nitrogen content Dataset Description: <p>This dataset includes carbon and nitrogen content data from Rhodomonas used in copepod experiments with varying pCO2 levels, from 2015.</p> Methods and Sampling: <p>The phytoplankton Rhodomonas sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (800ppmv), and High (1000ppmv).&nbsp; Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients.&nbsp; Some of the culture removed was used to evaluate chemical parameters.&nbsp; Samples for particulate cellular carbon and nitrogen were taken by gently vacuum filtering 100 ml from each pCO2 treatment replicate onto 21 mm muffled glass fiber (GF/F) filters.&nbsp; After filtration, filters were removed and placed in tin boats.&nbsp; Samples and controls (media blanks, filter blanks and capsule blanks) were placed in a drying oven for 24 hours at 60 °C, after which time they were removed and placed in a desiccator until analysis. Tin boats containing the filters and controls were folded into pellets, and then combusted using a Micro Cube elemental analyzer interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer at the UC Davis Stable Isotope Facility.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1220664 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1220664
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1220381 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1220381
completed
M. Brady Olson
Western Washington University
(360) 650-7400
1900 Shannon Point Rd.
Anacortes
WA
98221
United States
Brady.Olson@wwu.edu
pointOfContact
Julie E. Keister
University of Washington
206-543-7620
School of Oceanography Box 357940
Seattle
WA
98195
United States
jkeister@uw.edu
pointOfContact
Brooke Love
Western Washington University
(360) 650-2894
516 High St. Mail Stop 9181
Bellingham
WA
98225
United States
brooke.love@wwu.edu
pointOfContact
asNeeded
Unknown
temp
treatment
date
TOC_ug
TON_ug
C_per_cell_pg
C_to_N
Micro Cube elemental analyzer
PDZ Europa 20-20 isotope ratio mass spectrometer
theme
None, User defined
water temperature at measurement depth
treatment
date
total organic Carbon
total organic Nitrogen
Carbon to Nitrogen ratio (C:N)
featureType
BCO-DMO Standard Parameters
CHN Elemental Analyzer
Isotope-ratio Mass Spectrometer
instrument
BCO-DMO Standard Instruments
Lab_Olson_B
service
Deployment Activity
Bellingham, WA
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=503477
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
NSF Climate Research Investment (CRI) activities that were initiated in 2010 are now included under Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES). SEES is a portfolio of activities that highlights NSF's unique role in helping society address the challenge(s) of achieving sustainability. Detailed information about the SEES program is available from NSF (https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=504707).
In recognition of the need for basic research concerning the nature, extent and impact of ocean acidification on oceanic environments in the past, present and future, the goal of the SEES: OA program is to understand (a) the chemistry and physical chemistry of ocean acidification; (b) how ocean acidification interacts with processes at the organismal level; and (c) how the earth system history informs our understanding of the effects of ocean acidification on the present day and future ocean.
Solicitations issued under this program:
NSF 10-530, FY 2010-FY2011
NSF 12-500, FY 2012
NSF 12-600, FY 2013
NSF 13-586, FY 2014
NSF 13-586 was the final solicitation that will be released for this program.
PI Meetings:
1st U.S. Ocean Acidification PI Meeting(March 22-24, 2011, Woods Hole, MA)
2nd U.S. Ocean Acidification PI Meeting(Sept. 18-20, 2013, Washington, DC)
3rd U.S. Ocean Acidification PI Meeting (June 9-11, 2015, Woods Hole, MA – Tentative)
NSF media releases for the Ocean Acidification Program:
Press Release 10-186 NSF Awards Grants to Study Effects of Ocean Acidification
Discovery Blue Mussels "Hang On" Along Rocky Shores: For How Long?
Discovery nsf.gov - National Science Foundation (NSF) Discoveries - Trouble in Paradise: Ocean Acidification This Way Comes - US National Science Foundation (NSF)
Press Release 12-179 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: Finding New Answers Through National Science Foundation Research Grants - US National Science Foundation (NSF)
Press Release 13-102 World Oceans Month Brings Mixed News for Oysters
Press Release 13-108 nsf.gov - National Science Foundation (NSF) News - Natural Underwater Springs Show How Coral Reefs Respond to Ocean Acidification - US National Science Foundation (NSF)
Press Release 13-148 Ocean acidification: Making new discoveries through National Science Foundation research grants
Press Release 13-148 - Video nsf.gov - News - Video - NSF Ocean Sciences Division Director David Conover answers questions about ocean acidification. - US National Science Foundation (NSF)
Press Release 14-010 nsf.gov - National Science Foundation (NSF) News - Palau's coral reefs surprisingly resistant to ocean acidification - US National Science Foundation (NSF)
Press Release 14-116 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: NSF awards $11.4 million in new grants to study effects on marine ecosystems - US National Science Foundation (NSF)
SEES-OA
largerWorkCitation
program
Impacts on copepod populations mediated by changes in prey quality
https://osprey.bco-dmo.org/project/2218
Impacts on copepod populations mediated by changes in prey quality
<p>Research shows that ocean acidification (OA) has physiological consequences for individual organisms, even those lacking calcium carbonate skeletal structures. However, this existing research does not adequately address how OA effects to individuals are linked across trophic levels. Pelagic copepods are critical players in most marine biogeochemical cycles. Their consumption of phytoplankton and microzooplankton is the primary mechanism by which bacterial and phytoplankton production is transferred to higher trophic levels. Despite their high abundance and ecological importance, copepods have received little research attention concerning OA. The few extant studies focused on direct acute effects to copepods (e.g. egg hatching, survival) under elevated pCO2, and few significant effects have been observed at predicted future pCO2. However, there is increasing recognition that OA significantly affects their phytoplankton prey, including elevating growth rates, increasing cell sizes, altering nutrient uptake and ratios, and chemical composition. Because copepod grazing, egg production, and hatching success all can vary with these prey characteristics, OA mediated changes in phytoplankton quality may be an important indirect mechanism through which OA acts on copepod populations and, ultimately, marine food webs.</p>
<p>This study that will advance our understanding of how copepod populations may be affected by OA, specifically through OA induced changes in phytoplankton quality. Our core objective is to determine how changes in phytoplankton physiology and biochemistry (e.g. lipid composition) affect copepod egg production, hatching, and ontogenetic development of nauplii. We will also include a subset of experiments to test whether OA affects copepod reproductive output independent of changes to prey. To achieve these research goals, the diatom, <em>Ditylum brightwellii</em>, and dinoflagellate, <em>Prorocentrum micans</em>, will be cultured semi-continuously under several pCO2 concentrations, during which time we will characterize changes in their physiology and biochemistry. The copepods, <em>Calanus pacificus</em>, a large, high lipid-bearing marine species, and <em>Acartia clausi</em>, a smaller, low lipid-bearing estuarine species, will be maintained across varying pCO2 concentrations and fed these pCO2-acclimated prey, and their grazing and reproductive capability quantified. The copepods and phytoplankton used in this study will be collected from the Salish Sea, a region already experiencing periods of high pCO2/H+ (>1000 ppm, pH 7.5) on varying timescales. Therefore, this research addresses a question of how future climate change may impact marine ecosystems, but also is relevant to pCO2/H+ variability presently experienced in coastal environments.</p>
OA-Copepod_PreyQual
largerWorkCitation
project
eng; USA
biota
oceans
Bellingham, WA
2017-01-18
Puget Sound, Salish Sea
0
BCO-DMO catalogue of parameters from Carbon and nitrogen content of Rhodomonas cells grown with varying CO2 levels for copepod experiments, 2015 (OA-Copepod_PreyQual project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/675254.rdf
Name: temp
Units: degrees Celsius
Description: <p>Temperature at which the Rhodomonas were grown</p>
http://lod.bco-dmo.org/id/dataset-parameter/675255.rdf
Name: treatment
Units: unitless
Description: <p>CO2 level for growth conditions (ambient = 400; moderate = 800; high = 1200)</p>
http://lod.bco-dmo.org/id/dataset-parameter/675256.rdf
Name: date
Units: unitless
Description: <p>date of sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/675257.rdf
Name: TOC_ug
Units: micrograms
Description: <p>Total micrograms of Carbon in sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/675258.rdf
Name: TON_ug
Units: micrograms
Description: <p>Total micrograms of Nitrogen in sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/675259.rdf
Name: C_per_cell_pg
Units: picograms
Description: <p>picograms of Carbon per cell in sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/675260.rdf
Name: C_to_N
Units: dimensionless
Description: <p>ratio of Carbon to Nitrogen in sample</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
3143
https://datadocs.bco-dmo.org/file/VJJWqmQt2EjJZ/Rhodo_CN.csv
Rhodo_CN.csv
Primary data file for dataset ID 675242
download
https://osprey.bco-dmo.org/dataset/675242/data/download
download
onLine
dataset
<p>The phytoplankton Rhodomonas sp. CCMP 755 was grown semi-continuously in atmosphere controlled chambers at three different CO2 treatment concentrations; Ambient (400ppmv), Moderate (800ppmv), and High (1000ppmv).&nbsp; Cultures were diluted daily starting day 4 with pre-equilibrated media containing f/50 nutrients.&nbsp; Some of the culture removed was used to evaluate chemical parameters.&nbsp; Samples for particulate cellular carbon and nitrogen were taken by gently vacuum filtering 100 ml from each pCO2 treatment replicate onto 21 mm muffled glass fiber (GF/F) filters.&nbsp; After filtration, filters were removed and placed in tin boats.&nbsp; Samples and controls (media blanks, filter blanks and capsule blanks) were placed in a drying oven for 24 hours at 60 °C, after which time they were removed and placed in a desiccator until analysis. Tin boats containing the filters and controls were folded into pellets, and then combusted using a Micro Cube elemental analyzer interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer at the UC Davis Stable Isotope Facility.</p>
Specified by the Principal Investigator(s)
<p>The data are unprocessed.</p>
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- replaced spaces with underscore&nbsp;</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Micro Cube elemental analyzer
Micro Cube elemental analyzer
PI Supplied Instrument Name: Micro Cube elemental analyzer Instrument Name: CHN Elemental Analyzer Instrument Short Name:CHN_EA Instrument Description: A CHN Elemental Analyzer is used for the determination of carbon, hydrogen, and nitrogen content in organic and other types of materials, including solids, liquids, volatile, and viscous samples.
PDZ Europa 20-20 isotope ratio mass spectrometer
PDZ Europa 20-20 isotope ratio mass spectrometer
PI Supplied Instrument Name: PDZ Europa 20-20 isotope ratio mass spectrometer Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Deployment: Lab_Olson_B
Lab_Olson_B
WWU
laboratory
WWU
laboratory