http://lod.bco-dmo.org/id/dataset/683072
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2017-02-24
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Environmental data collected in marine lakes in Palau in 2010 from small boats
2017-02-24
publication
2017-02-24
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-08-06
publication
https://doi.org/10.1575/1912/bco-dmo.683072.1
John Michael Beman
University of California-Merced
principalInvestigator
Michael N. Dawson
University of California-Merced
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Beman, J., Dawson, M. (2017) Environmental data collected in marine lakes in Palau in 2010 from small boats. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2017-02-24 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.683072.1 [access date]
Environmental data collected in marine lakes in Palau in 2010 Dataset Description: <p>Environmental data collected in marine lakes in Palau in 2010.</p> Methods and Sampling: <p>Refer to the following paper for complete methodology:<br />
Meyerhof, M. et al. 2016.&nbsp;Microbial community diversity, structure and assembly across oxygen gradients in meromictic marine lakes, Palau. Environmental Microbiology. doi:<a href="http://dx.doi.org/10.1111/1462-2920.13416" target="_blank">10.1111/1462-2920.13416</a></p>
<p>In summary (extracted from above paper):<br />
We studied five meromictic lakes in Palau: Spooky Lake (SLM), Goby Lake (GLK), Ongeim’l Tketau Lake (OTM, known colloquially as Jellyfish Lake), Clear Lake (CLM) and Ngermeuangel Lake (NLK). One holomictic lake (Mekeald Lake; MLN) and an ocean site at the German Channel on the southwestern side of the islands (OS-GC) were also sampled for comparison. We vertically profiled dissolved oxygen (DO), temperature, pH, chlorophyll fluorescence and salinity/conductivity using a HydroLab DS5 (Hach Company, Loveland, CO, USA) and sampled three depth layers within each meromictic lake: the mixolimnion (0–5 m depth), the monimolimnion (5–20 m depth) and intermediate depths near the chemocline; these intermediate depths ranged from 1 to 15 m, depending on the depth of the chemocline within the individual lakes. We sampled comparable depths at MLN (5–20 m) and OS-GC (5–30 m). For QPCR analysis of functional genes (dsrA, amoA and nirS) and specific functional groups, as well as analysis dissolved nutrient concentrations, we collected additional samples above and below the chemocline to capture abrupt transitions in biogeochemical conditions across this interface.</p>
<p>Samples were collected from small boats using a horizontal, 2.5 L GoFlo bottle (General Oceanics, Miami, FL, USA), transferred to 1 L polycarbonate bottles, and stored in the dark during transit to the Coral Reef Research Foundation laboratory in Koror, Palau. Water samples were filtered using a peristaltic pump and 0.22 um Durapore PVDF hydrophilic filters (Millipore, Billerica, MA, USA). Filters were immediately frozen in 800 uL Sucrose-Tris-EDTA (STE) lysis buffer (750 mM sucrose, 20 mM EDTA, 400 mM NaCl and 50 mM Tris) in 2 mL Lysing Matrix E tubes (MP Biomedicals, Solon, OH, USA), and stored at -20 C until transport to the United States, where they were stored at -80 C until extraction (Dry ice and liquid nitrogen are not readily available in Palau.)</p>
<p>During sample filtration, 50 mL of filtrate was collected in high density polyethylene bottles for subsequent nutrient analysis at the University of California, Santa Barbara (UCSB) Marine Analytical Laboratory. Samples were analyzed for ammonium (UCSB MAL analytical method for ammonium, see below; Diamond and Huberty, 1996), nitrite (Environmental Protection Agency (EPA) Method 353.2; Schroeder, 1997), nitrite+nitrate (EPA Method 353.2; Diamond, 1997) and phosphate (EPA Method 365.1; Huberty and Diamond, 1998), on a Lachat QuikChem 8000 Flow Injection Analyzer (Hach Company, Loveland, CO, USA). A handful of samples containing large concentrations of sulfide were not analyzed for nitrate, as sulfide damages the cadmium reduction column. For ammonium analysis, each sample was injected into a flowing carrier stream through an injection valve, and then merged with an alkaline solution stream; the produced ammonia was diffused through a hydrophobic, gas-permeable membrane into a recipient stream containing a pH indicator. Colour change occurs in the indicator solution due to an increase in pH, and the concentration of ammonia was determined spectrophotometrically based on absorption at 570 nm. For all analyses, a mid-range check standard bracketed every 20 samples to verify the accuracy of the measurements, and samples that were detected outside of the standards’ range were diluted 1:10 and reanalyzed. Detection limits were 0.10 uM for phosphate, 0.10 uM for nitrite, 0.20 uM for nitrite+nitrate and 0.10 uM for ammonium.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1241255 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1241255
completed
John Michael Beman
University of California-Merced
650-823-7925
5200 North Lake Road
Merced
CA
95343
USA
jmbeman@gmail.com
pointOfContact
Michael N. Dawson
University of California-Merced
209-228-4056
School of Natural Sciences 5200 North Lake Road
Merced
CA
95343
United States
mdawson@ucmerced.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
sample
lake
date
lat
lon
depth
temp
cond
sal
O2_diss
pH
chl
phosphate
nitrite
ammonium
nitrate
Lachat QuikChem 8000 Flow Injection Analyzer
GoFlo bottle
HydroLab DS5
theme
None, User defined
sample identification
site
date
latitude
longitude
depth
water temperature at measurement depth
conductivity
salinity
dissolved Oxygen
pH
No BCO-DMO term
reactive phosphorus (PO4)
Nitrite
Ammonium
Nitrate
featureType
BCO-DMO Standard Parameters
Flow Injection Analyzer
GO-FLO Bottle
Hydrolab Series 5 probes
instrument
BCO-DMO Standard Instruments
Palau_lakes
service
Deployment Activity
NW Pacific
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Dimensions of Biodiversity
http://www.nsf.gov/funding/pgm_summ.jsp?pims_id=503446
Dimensions of Biodiversity
(adapted from the NSF Synopsis of Program)
Dimensions of Biodiversity is a program solicitation from the NSF Directorate for Biological Sciences. FY 2010 was year one of the program. [MORE from NSF]
The NSF Dimensions of Biodiversity program seeks to characterize biodiversity on Earth by using integrative, innovative approaches to fill rapidly the most substantial gaps in our understanding. The program will take a broad view of biodiversity, and in its initial phase will focus on the integration of genetic, taxonomic, and functional dimensions of biodiversity. Project investigators are encouraged to integrate these three dimensions to understand the interactions and feedbacks among them. While this focus complements several core NSF programs, it differs by requiring that multiple dimensions of biodiversity be addressed simultaneously, to understand the roles of biodiversity in critical ecological and evolutionary processes.
Dimensions of Biodiversity
largerWorkCitation
program
Do Parallel Patterns Arise from Parallel Processes?
http://marinelakes.ucmerced.edu/
Do Parallel Patterns Arise from Parallel Processes?
<p>This project will survey the taxonomic, genetic, and functional diversity of the organisms found in marine lakes, and investigate the processes that cause gains and losses in this biodiversity. Marine lakes formed as melting ice sheets raised sea level after the last glacial maximum and flooded hundreds of inland valleys around the world. Inoculated with marine life from the surrounding sea and then isolated to varying degrees for the next 6,000 to 15,000 years, these marine lakes provide multiple, independent examples of how environments and interactions between species can drive extinction and speciation. Researchers will survey the microbes, algae, invertebrates, and fishes present in 40 marine lakes in Palau and Papua, and study how diversity has changed over time by retrieving the remains of organisms preserved in sediments on the lake bottoms. The project will test whether the number of species, the diversity of functional roles played by organisms, and the genetic diversity within species increase and decrease in parallel; whether certain species can greatly curtail diversity by changing the environment; whether the size of a lake determines its biodiversity; and whether the processes that control diversity in marine organisms are similar to those that operate on land.</p>
<p>Because biodiversity underlies the ecosystem services on which society depends, society has a great interest in understanding the processes that generate and retain biodiversity in nature. This project will also help conserve areas of economic importance. Marine lakes in the study region are important for tourism, and researchers will work closely with governmental and non-governmental conservation and education groups and with diving and tourism businesses to raise awareness of the value and threats to marine lakes in Indonesia and Palau.</p>
PaPaPro
largerWorkCitation
project
eng; USA
oceans
NW Pacific
134.269367
134.50855
7.1165
7.32285
2010-08-21
2010-09-03
Western Pacific; Palau; Indonesia (West Papua)
0
BCO-DMO catalogue of parameters from Environmental data collected in marine lakes in Palau in 2010 from small boats
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/683079.rdf
Name: sample
Units: unitless
Description: <p>Sample identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/683080.rdf
Name: lake
Units: unitless
Description: <p>Lake name</p>
http://lod.bco-dmo.org/id/dataset-parameter/683081.rdf
Name: date
Units: unitless
Description: <p>Date sampled, formatted as yyyy-mm-dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/683082.rdf
Name: lat
Units: decimal degrees
Description: <p>Latitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/683083.rdf
Name: lon
Units: decimal degrees
Description: <p>Longitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/683084.rdf
Name: depth
Units: meters (m)
Description: <p>Depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/683085.rdf
Name: temp
Units: degrees Celsius
Description: <p>Temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/683086.rdf
Name: cond
Units: milliSiemens per centimeter (mS/cm)
Description: <p>Conductivity</p>
http://lod.bco-dmo.org/id/dataset-parameter/683087.rdf
Name: sal
Units: ppt
Description: <p>Salinity</p>
http://lod.bco-dmo.org/id/dataset-parameter/683088.rdf
Name: O2_diss
Units: micromolar (uM)
Description: <p>Dissolved oxygen</p>
http://lod.bco-dmo.org/id/dataset-parameter/683089.rdf
Name: pH
Units: unitless
Description: <p>pH</p>
http://lod.bco-dmo.org/id/dataset-parameter/683090.rdf
Name: chl
Units: micrograms per liter (ug/L)
Description: <p>Chlorophyll</p>
http://lod.bco-dmo.org/id/dataset-parameter/683091.rdf
Name: phosphate
Units: micromolar (uM)
Description: <p>Phosphate</p>
http://lod.bco-dmo.org/id/dataset-parameter/683092.rdf
Name: nitrite
Units: micromolar (uM)
Description: <p>Nitrite</p>
http://lod.bco-dmo.org/id/dataset-parameter/683093.rdf
Name: ammonium
Units: micromolar (uM)
Description: <p>Ammonium</p>
http://lod.bco-dmo.org/id/dataset-parameter/683094.rdf
Name: nitrate
Units: micromolar (uM)
Description: <p>Nitrate</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2340
https://darchive.mblwhoilibrary.org/bitstream/1912/24423/1/dataset-683072_palau-environmental-2010__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.683072.1
download
onLine
dataset
<p>Refer to the following paper for complete methodology:<br />
Meyerhof, M. et al. 2016.&nbsp;Microbial community diversity, structure and assembly across oxygen gradients in meromictic marine lakes, Palau. Environmental Microbiology. doi:<a href="http://dx.doi.org/10.1111/1462-2920.13416" target="_blank">10.1111/1462-2920.13416</a></p>
<p>In summary (extracted from above paper):<br />
We studied five meromictic lakes in Palau: Spooky Lake (SLM), Goby Lake (GLK), Ongeim’l Tketau Lake (OTM, known colloquially as Jellyfish Lake), Clear Lake (CLM) and Ngermeuangel Lake (NLK). One holomictic lake (Mekeald Lake; MLN) and an ocean site at the German Channel on the southwestern side of the islands (OS-GC) were also sampled for comparison. We vertically profiled dissolved oxygen (DO), temperature, pH, chlorophyll fluorescence and salinity/conductivity using a HydroLab DS5 (Hach Company, Loveland, CO, USA) and sampled three depth layers within each meromictic lake: the mixolimnion (0–5 m depth), the monimolimnion (5–20 m depth) and intermediate depths near the chemocline; these intermediate depths ranged from 1 to 15 m, depending on the depth of the chemocline within the individual lakes. We sampled comparable depths at MLN (5–20 m) and OS-GC (5–30 m). For QPCR analysis of functional genes (dsrA, amoA and nirS) and specific functional groups, as well as analysis dissolved nutrient concentrations, we collected additional samples above and below the chemocline to capture abrupt transitions in biogeochemical conditions across this interface.</p>
<p>Samples were collected from small boats using a horizontal, 2.5 L GoFlo bottle (General Oceanics, Miami, FL, USA), transferred to 1 L polycarbonate bottles, and stored in the dark during transit to the Coral Reef Research Foundation laboratory in Koror, Palau. Water samples were filtered using a peristaltic pump and 0.22 um Durapore PVDF hydrophilic filters (Millipore, Billerica, MA, USA). Filters were immediately frozen in 800 uL Sucrose-Tris-EDTA (STE) lysis buffer (750 mM sucrose, 20 mM EDTA, 400 mM NaCl and 50 mM Tris) in 2 mL Lysing Matrix E tubes (MP Biomedicals, Solon, OH, USA), and stored at -20 C until transport to the United States, where they were stored at -80 C until extraction (Dry ice and liquid nitrogen are not readily available in Palau.)</p>
<p>During sample filtration, 50 mL of filtrate was collected in high density polyethylene bottles for subsequent nutrient analysis at the University of California, Santa Barbara (UCSB) Marine Analytical Laboratory. Samples were analyzed for ammonium (UCSB MAL analytical method for ammonium, see below; Diamond and Huberty, 1996), nitrite (Environmental Protection Agency (EPA) Method 353.2; Schroeder, 1997), nitrite+nitrate (EPA Method 353.2; Diamond, 1997) and phosphate (EPA Method 365.1; Huberty and Diamond, 1998), on a Lachat QuikChem 8000 Flow Injection Analyzer (Hach Company, Loveland, CO, USA). A handful of samples containing large concentrations of sulfide were not analyzed for nitrate, as sulfide damages the cadmium reduction column. For ammonium analysis, each sample was injected into a flowing carrier stream through an injection valve, and then merged with an alkaline solution stream; the produced ammonia was diffused through a hydrophobic, gas-permeable membrane into a recipient stream containing a pH indicator. Colour change occurs in the indicator solution due to an increase in pH, and the concentration of ammonia was determined spectrophotometrically based on absorption at 570 nm. For all analyses, a mid-range check standard bracketed every 20 samples to verify the accuracy of the measurements, and samples that were detected outside of the standards’ range were diluted 1:10 and reanalyzed. Detection limits were 0.10 uM for phosphate, 0.10 uM for nitrite, 0.20 uM for nitrite+nitrate and 0.10 uM for ammonium.</p>
Specified by the Principal Investigator(s)
<p>BCO-DMO Processing:<br />
-modified parameter names to conform with BCO-DMO naming conventions;<br />
-formatted date to yyyy-mm-dd.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Lachat QuikChem 8000 Flow Injection Analyzer
Lachat QuikChem 8000 Flow Injection Analyzer
PI Supplied Instrument Name: Lachat QuikChem 8000 Flow Injection Analyzer PI Supplied Instrument Description:Samples were analyzed for ammonium, nitrite, nitrite+nitrate, and phosphate on a Lachat QuikChem 8000 Flow Injection Analyzer (Hach Company, Loveland, CO, USA). Instrument Name: Flow Injection Analyzer Instrument Short Name:FIA Instrument Description: An instrument that performs flow injection analysis. Flow injection analysis (FIA) is an approach to chemical analysis that is accomplished by injecting a plug of sample into a flowing carrier stream. FIA is an automated method in which a sample is injected into a continuous flow of a carrier solution that mixes with other continuously flowing solutions before reaching a detector. Precision is dramatically increased when FIA is used instead of manual injections and as a result very specific FIA systems have been developed for a wide array of analytical techniques. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB36/
GoFlo bottle
GoFlo bottle
PI Supplied Instrument Name: GoFlo bottle PI Supplied Instrument Description:Samples were collected from small boats using a horizontal, 2.5 L GoFlo bottle (General Oceanics, Miami, FL, USA). Instrument Name: GO-FLO Bottle Instrument Short Name:GO-FLO Instrument Description: GO-FLO bottle cast used to collect water samples for pigment, nutrient, plankton, etc. The GO-FLO sampling bottle is specially designed to avoid sample contamination at the surface, internal spring contamination, loss of sample on deck (internal seals), and exchange of water from different depths. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/30/
HydroLab DS5
HydroLab DS5
PI Supplied Instrument Name: HydroLab DS5 PI Supplied Instrument Description:Dissolved oxygen (DO), temperature, pH, chlorophyll fluorescence and salinity/conductivity were measured using a HydroLab DS5 Multiparameter Data Sonde (Hach Company, Loveland, CO, USA). Instrument Name: Hydrolab Series 5 probes Instrument Short Name:HydroLab DS5 Instrument Description: Multi-parameter probes that can measure from 12 (MS5) to 16 (DS5 and DS5X) parameters simultaneously. Measurements include temperature, depth, conductivity, salinity, specific conductance, TDS, pH, ORP, dissolved oxygen, turbidity, chlorophyll a, blue-green algae, Rhodamine WT, ammonium, nitrate, chloride, PAR and total dissolved gases. These probes can be deployed at depths up to 200 m and can be used in continuous monitoring programs.
Cruise: Palau_lakes
Palau_lakes
Small boats - CRRF
vessel
Palau_lakes
Michael N. Dawson
University of California-Merced
Small boats - CRRF
vessel