http://lod.bco-dmo.org/id/dataset/684199
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2017-03-13
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Animal condition data from Pleuromamma xiphias (Atlantic Ocean, AMT24) (Plankton Population Genetics project)
2017-03-13
publication
2017-03-13
revision
BCO-DMO Linked Data URI
2017-03-13
creation
http://lod.bco-dmo.org/id/dataset/684199
Erica Goetze
University of Hawaiʻi at Mānoa
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Goetze, E. (2017) Animal condition data from Pleuromamma xiphias (Atlantic Ocean, AMT24) (Plankton Population Genetics project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2017-03-13 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/684199 [access date]
P. xiphias AMT24 condition Dataset Description: <p>This dataset includes animal condition&nbsp;data for individual Pleuromamma xiphias specimens collected across ocean provinces&nbsp;on Atlantic Meridional Transect Cruise 24 (AMT24) in October&nbsp;2014. Reported measurements include copepod prosome length (PL), wet and dry&nbsp;weight, carbon and nitrogen content, delta-15N and delta-13C stable isotope values, C:N ratio, and condition factor index (CFI).</p>
<p>Dataset release date is approximate.</p> Methods and Sampling: <p><strong>Field collection.</strong> Adults of Pleuromamma xiphias were collected on the Atlantic Meridional Transect Cruise 24 (AMT24), between 4 Oct and 28 Oct 2014 (Fig. 1, Table S1). Animals from 20 stations along the cruise transect between 31°N and 43°S were included in this study. Oblique bongo tows (200 mm nets) were conducted between on average 310 m and the sea surface. Upon recovery at the surface, plankton from one net was diluted into chilled seawater, and sorted immediately. &nbsp;At some stations, plankton was split quantitatively in a Folsom plankton splitter prior to sorting. Adult females of Pleuromamma xiphias were sorted live under the microscope, flash frozen and stored in liquid nitrogen at sea, followed by transfer to -80°C in the laboratory. Only adult females were included in this study, in order to ensure comparable measurements across the oceanographic transect. &nbsp;Adult males have larger body size than females (Bradford-Grieve 1999), and are expected to have higher carbon and nitrogen content as well as dry weight relative to females.</p>
<p>Cryopreserved Pleuromamma xiphias females were stored on ice during all initial handling steps, with 5-10 animals processed at a time. Prosome length (mm) of each animal was measured in right lateral view.&nbsp; Individual females were then blotted dry and transferred to pre-weighed tin capsules for wet weight and dry weight measurements. Dry weight: copepods were individually weighed using a Mettler Toledo UMX2 ultramicrobalance, dried at 60°C for 24-72 hours, and weighed again. &nbsp;</p>
<p>Carbon and Nitrogen content: One or two individual copepods were included in elemental and isotope analyses, depending on dry weight.&nbsp; The C content, N content and bulk isotope composition of P. xiphias was determined using a Costech elemental combustion system (Model 4010) coupled to a ThermoFinnigan Delta Plus XP Isotope Ratio Mass Spectrometer (IRMS) through a Conflo IV interface.&nbsp; Isotope values are reported in standard δ-notation relative to the atmospheric N2 standard (AIR) as δ15N (‰) = [(15N:14Nsample / 15N:14NAIR) - 1] x 1000 or relative to Vienna Pee Dee Belemnite (VPDB) as δ13C (‰) = [(13C:12Csample / 13C:12CVPDB) - 1] x 1000.&nbsp; To ensure accuracy, glycine and ground tuna reference samples with well-characterized δ15N values were analyzed every 10 samples.&nbsp; The standard deviation of samples analyzed in duplicate was ≤ 0.2‰. Body size and weight measurements were made on a total of 237 animals, with 106 carbon and nitrogen content and isotopic measurements made across the transect.</p>
<p>Condition index (CFI) defined as CFI = 1000 x DW/(PL)3 (Ikeda et al. 2006). Stable isotope values measured on single individuals or in pools of N=2 (as noted in the SIA identifier column).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1338959 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1338959
completed
Erica Goetze
University of Hawaiʻi at Mānoa
808-956-7156
Department of Oceanography, University of Hawaii at Manoa 1000 Pope Road
Honolulu
HI
96822
United States
egoetze@hawaii.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
sample
cruise_id
station
lat
lon
date
wet_wgt_mg
dry_wgt_mg
len_prosome
CFI
water_content
SIA_id
delta_15N
delta_13C
wgt_animal_mg
N_animal_ug
C_animal_ug
C_to_N
Costech elemental combustion system (Model 4010)
ThermoFinnigan Delta Plus XP Isotope Ratio Mass Spectrometer (IRMS)
Mettler Toledo UMX2 ultramicrobalance
theme
None, User defined
sample identification
Cruise identfier
station number
latitude
longitude
date
weight
dry_wgt
length
No BCO-DMO term
d15N measured in biota
d13C measured in biota
total organic Nitrogen
total organic Carbon
Carbon to Nitrogen ratio (C:N)
featureType
BCO-DMO Standard Parameters
Elemental Analyzer
Isotope-ratio Mass Spectrometer
scale or balance
instrument
BCO-DMO Standard Instruments
AMT24
service
Deployment Activity
Immingham, United Kingdom to Punta Arenas, Chile
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Basin-scale genetics of marine zooplankton
https://osprey.bco-dmo.org/project/537991
Basin-scale genetics of marine zooplankton
<p><em>Description from NSF award abstract:</em><br />
Marine zooplankton show strong ecological responses to climate change, but little is known about their capacity for evolutionary response. Many authors have assumed that the evolutionary potential of zooplankton is limited. However, recent studies provide circumstantial evidence for the idea that selection is a dominant evolutionary force acting on these species, and that genetic isolation can be achieved at regional spatial scales in pelagic habitats. This RAPID project will take advantage of a unique opportunity for basin-scale transect sampling through participation in the Atlantic Meridional Transect (AMT) cruise in 2014. The cruise will traverse more than 90 degrees of latitude in the Atlantic Ocean and include boreal-temperate, subtropical and tropical waters. Zooplankton samples will be collected along the transect, and mitochondrial and microsatellite markers will be used to identify the geographic location of strong genetic breaks within three copepod species. Bayesian and coalescent analytical techniques will test if these regions act as dispersal barriers. The physiological condition of animals collected in distinct ocean habitats will be assessed by measurements of egg production (at sea) as well as body size (condition index), dry weight, and carbon and nitrogen content. The PI will test the prediction that ocean regions that serve as dispersal barriers for marine holoplankton are areas of poor-quality habitat for the target species, and that this is a dominant mechanism driving population genetic structure in oceanic zooplankton.</p>
<p>Note: This project is funded by an NSF RAPID award. This RAPID grant supported the shiptime costs, and all the sampling reported in the <a href="http://dmoserv3.whoi.edu/data_docs/Goetze/AMT24_cruise/GOETZE_AMT24_Cruise_Report.pdf" target="_blank">AMT24 zooplankton ecology cruise report (PDF)</a>.</p>
<p>Online science outreach blog at: <a href="https://atlanticplankton.wordpress.com" target="_blank">https://atlanticplankton.wordpress.com</a></p>
Plankton Population Genetics
largerWorkCitation
project
eng; USA
biota
oceans
Immingham, United Kingdom to Punta Arenas, Chile
-37.137
-25.015
-43.02
31.87
2014-10-02
2014-10-28
Atlantic Ocean, 46 N - 46 S
0
BCO-DMO catalogue of parameters from Animal condition data from Pleuromamma xiphias (Atlantic Ocean, AMT24) (Plankton Population Genetics project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/684281.rdf
Name: sample
Units: unitless
Description: <p>sample identifier: incrementing number of individuals included in the study</p>
http://lod.bco-dmo.org/id/dataset-parameter/684282.rdf
Name: cruise_id
Units: unitless
Description: <p>cruise identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/684283.rdf
Name: station
Units: unitless
Description: <p>station identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/684284.rdf
Name: lat
Units: decimal degrees
Description: <p>latitude; north is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/684285.rdf
Name: lon
Units: decimal degrees
Description: <p>longitude; east is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/684286.rdf
Name: date
Units: unitless
Description: <p>collection date (UTC) formatted as yyyy-mm-dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/684287.rdf
Name: wet_wgt_mg
Units: milligrams (mg)
Description: <p>wet weight</p>
http://lod.bco-dmo.org/id/dataset-parameter/684288.rdf
Name: dry_wgt_mg
Units: milligrams (mg)
Description: <p>dry weight</p>
http://lod.bco-dmo.org/id/dataset-parameter/684289.rdf
Name: len_prosome
Units: millimeters (mm)
Description: <p>prosome Length measured in right lateral view</p>
http://lod.bco-dmo.org/id/dataset-parameter/684290.rdf
Name: CFI
Units: unitless
Description: <p>Condition Factor Index: CFI = 1000 x DW/(PL)3 -- (Ikeda et al. 2006)</p>
http://lod.bco-dmo.org/id/dataset-parameter/684291.rdf
Name: water_content
Units: unitless
Description: <p>percent water content</p>
http://lod.bco-dmo.org/id/dataset-parameter/684292.rdf
Name: SIA_id
Units: unitless
Description: <p>Stable Isotope Analysis identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/684293.rdf
Name: delta_15N
Units: unitless
Description: <p>delta 15N; the ratio of stable isotopes 15N:14N (o/oo vs. AIR)</p>
http://lod.bco-dmo.org/id/dataset-parameter/684294.rdf
Name: delta_13C
Units: unitless
Description: <p>delta 13C; the ratio of stable isotopes 13C:12C</p>
http://lod.bco-dmo.org/id/dataset-parameter/684295.rdf
Name: wgt_animal_mg
Units: milligrams (mg)
Description: <p>wet weight per specimen</p>
http://lod.bco-dmo.org/id/dataset-parameter/684296.rdf
Name: N_animal_ug
Units: micrograms (ug)
Description: <p>Nitrogen content per animal</p>
http://lod.bco-dmo.org/id/dataset-parameter/684297.rdf
Name: C_animal_ug
Units: micrograms (ug)
Description: <p>Carbon content per animal</p>
http://lod.bco-dmo.org/id/dataset-parameter/684298.rdf
Name: C_to_N
Units: unitless
Description: <p>Carbon to Nitrogen ratio</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://osprey.bco-dmo.org/dataset/684199/data/download
download
onLine
dataset
<p><strong>Field collection.</strong> Adults of Pleuromamma xiphias were collected on the Atlantic Meridional Transect Cruise 24 (AMT24), between 4 Oct and 28 Oct 2014 (Fig. 1, Table S1). Animals from 20 stations along the cruise transect between 31°N and 43°S were included in this study. Oblique bongo tows (200 mm nets) were conducted between on average 310 m and the sea surface. Upon recovery at the surface, plankton from one net was diluted into chilled seawater, and sorted immediately. &nbsp;At some stations, plankton was split quantitatively in a Folsom plankton splitter prior to sorting. Adult females of Pleuromamma xiphias were sorted live under the microscope, flash frozen and stored in liquid nitrogen at sea, followed by transfer to -80°C in the laboratory. Only adult females were included in this study, in order to ensure comparable measurements across the oceanographic transect. &nbsp;Adult males have larger body size than females (Bradford-Grieve 1999), and are expected to have higher carbon and nitrogen content as well as dry weight relative to females.</p>
<p>Cryopreserved Pleuromamma xiphias females were stored on ice during all initial handling steps, with 5-10 animals processed at a time. Prosome length (mm) of each animal was measured in right lateral view.&nbsp; Individual females were then blotted dry and transferred to pre-weighed tin capsules for wet weight and dry weight measurements. Dry weight: copepods were individually weighed using a Mettler Toledo UMX2 ultramicrobalance, dried at 60°C for 24-72 hours, and weighed again. &nbsp;</p>
<p>Carbon and Nitrogen content: One or two individual copepods were included in elemental and isotope analyses, depending on dry weight.&nbsp; The C content, N content and bulk isotope composition of P. xiphias was determined using a Costech elemental combustion system (Model 4010) coupled to a ThermoFinnigan Delta Plus XP Isotope Ratio Mass Spectrometer (IRMS) through a Conflo IV interface.&nbsp; Isotope values are reported in standard δ-notation relative to the atmospheric N2 standard (AIR) as δ15N (‰) = [(15N:14Nsample / 15N:14NAIR) - 1] x 1000 or relative to Vienna Pee Dee Belemnite (VPDB) as δ13C (‰) = [(13C:12Csample / 13C:12CVPDB) - 1] x 1000.&nbsp; To ensure accuracy, glycine and ground tuna reference samples with well-characterized δ15N values were analyzed every 10 samples.&nbsp; The standard deviation of samples analyzed in duplicate was ≤ 0.2‰. Body size and weight measurements were made on a total of 237 animals, with 106 carbon and nitrogen content and isotopic measurements made across the transect.</p>
<p>Condition index (CFI) defined as CFI = 1000 x DW/(PL)3 (Ikeda et al. 2006). Stable isotope values measured on single individuals or in pools of N=2 (as noted in the SIA identifier column).</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- converted latitude and longitude to decimal degrees<br />
- re-formatted date from m/d/yyyy to yyyy-mm-dd</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Costech elemental combustion system (Model 4010)
Costech elemental combustion system (Model 4010)
PI Supplied Instrument Name: Costech elemental combustion system (Model 4010) PI Supplied Instrument Description:Used to measure the C and N content of the copepods Instrument Name: Elemental Analyzer Instrument Short Name: Instrument Description: Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/
ThermoFinnigan Delta Plus XP Isotope Ratio Mass Spectrometer (IRMS)
ThermoFinnigan Delta Plus XP Isotope Ratio Mass Spectrometer (IRMS)
PI Supplied Instrument Name: ThermoFinnigan Delta Plus XP Isotope Ratio Mass Spectrometer (IRMS) PI Supplied Instrument Description:Coupled to the Elemental Analyzer through a Conflo IV interface to measure isotope values of individual copepods. Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Mettler Toledo UMX2 ultramicrobalance
Mettler Toledo UMX2 ultramicrobalance
PI Supplied Instrument Name: Mettler Toledo UMX2 ultramicrobalance PI Supplied Instrument Description:Used to weigh individual copepods Instrument Name: scale or balance Instrument Short Name: Instrument Description: Devices that determine the mass or weight of a sample. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB13/
Cruise: AMT24
AMT24
RRS James Clark Ross
Community Standard Description
International Council for the Exploration of the Sea
RRS James Clark Ross
vessel
AMT24
Dr Tim Smyth
Plymouth Marine Laboratory
http://dmoserv3.bco-dmo.org/data_docs/Goetze/AMT24_cruise/AMT24_jr303_cruise_report.pdf
Report describing AMT24
RRS James Clark Ross
Community Standard Description
International Council for the Exploration of the Sea
RRS James Clark Ross
vessel