<div><p>This data set is associated with PI Douglas Bartlett (NSF OCE-1536776) and Schmidt Ocean Institute R/V Falkor cruise FK141215. The cruise occurred December 15-21, 2014 in the Challenger Deep within the territorial waters of the Federated States of Micronesia. During this cruise the Leggo lander was deployed multiple times and drops 1 and 3 recovered seawater samples that were analyzed. Additional details can be found at: <a href="https://schmidtocean.org/cruise/expanding-mariana-trench-perspectives/" target="_blank">https://schmidtocean.org/cruise/expanding-mariana-trench-perspectives/</a> and <a href="https://scripps.ucsd.edu/labs/dbartlett/contact/challenger-deep-cruise-2014/" target="_blank">https://scripps.ucsd.edu/labs/dbartlett/contact/challenger-deep-cruise-2014/</a></p>
<p><strong>Leggo Lander Drop 1:</strong><br />
Time (in Guam) deployed/recovered: December 16, 9:00/19:26.<br />
Position at deployment: 11° 21.9836 N 142° 25.9533 E, middle section of the Challenger Deep.<br />
Greatest depth of dive: approximately ~10,900 m.<br /><em>In situ</em> temperature on seafloor: 2.6°C.<br />
Notes: This drop recovered seawater samples from about a meter off the seafloor. This included a 3 L Niskin bottle of seawater and ~ 150 mls of seawater collected in a pressure-retaining seawater sampler. The PRS sampler held more than 81% of the in situ pressure. </p>
<p><strong>PRS data:</strong><br />
The culture independent identification of the bacteria present in the pressure-retaining seawater (PRS) sampler deployed during Leggo drop 1. This involved cell sorting, multiple displacement amplification, and 16S rRNA gene PCR and sequencing. More complete details are described in:</p>
<p>Leon-Zayas, R., Novotny, M., Podell, S., Shepard, C. M., Berkenpas, E., Nikolenko, S., Pevzner, P., Lasken, R. S. and Bartlett, D. H. 2015. Single cells within the Puerto Rico Trench suggest hadal adaptation of microbial lineages. Appl. Environ. Microbiol. 81: 8265-8276. doi:<a href="https://dx.doi.org/10.1128/AEM.01659-15" target="_blank">10.1128/AEM.01659-15</a></p>
<p><strong>Colony Identification:</strong><br />
Data from the identification of bacteria cultured from the Leggo drop 1 and 3 Niskin bottles are available as a <a href="https://datadocs.bco-dmo.org/docs/bartlett/mariana_perspectives/data_docs/colony_identification.txt" target="_blank">supplemental file</a> (.txt). These identifications were performed using standard methods associated with PCR amplification of the 16S rRNA gene followed by dideoxy sequencing at Retrogen Inc. </p></div>
Culture-independent identification of bacteria present in the pressure-retaining seawater (PRS) sampler deployed during Leggo drop 1
<div><p>Culture-independent identification of bacteria present in the pressure-retaining seawater (PRS) sampler deployed during Leggo drop 1.</p></div>
PRS bacteria identification
<div><p>The only processing of the data is that the 16S rRNA gene sequences has been examined using BLAST: <a href="https://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome" target="_blank">https://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome</a></p>
<p>BCO-DMO Processing:<br />
-modified parameter names to conform with BCO-DMO naming conventions;<br />
-replaced spaces with underscores;<br />
-replaced '-' with 'nd' (no data);<br />
-added dates, times, locations from metadata form.</p></div>
684362
PRS bacteria identification
2017-03-14T15:34:20-04:00
2017-03-14T15:34:20-04:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:684362
Culture-independent identification of bacteria present in the pressure-retaining seawater (PRS) sampler deployed during Leggo drop 1 from R/V Falkor cruise FK141215 in the Challenger Deep, Mariana Trench in December 2014
Culture-independent identification of bacteria present in the pressure-retaining seawater (PRS) sampler deployed during Leggo drop 1.
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Bartlett, D. (2017) Culture-independent identification of bacteria present in the pressure-retaining seawater (PRS) sampler deployed during Leggo drop 1 from R/V Falkor cruise FK141215 in the Challenger Deep, Mariana Trench in December 2014. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2017-03-13 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.684362.1 [access date]
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