http://lod.bco-dmo.org/id/dataset/684417
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2017-03-16
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Sulfide concentrations as a function of time from INSPIRE track 1 collected from 2013 to 2017 (INSPIRE Pyrite project)
2017-03-13
publication
2017-03-13
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-06-11
publication
https://doi.org/10.1575/1912/bco-dmo.684417.1
Peter Girguis
Harvard University
principalInvestigator
David Clarke
Harvard University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Girguis, P., Clarke, D. (2017) Sulfide concentrations as a function of time from INSPIRE track 1 collected from 2013 to 2017 (INSPIRE Pyrite project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2017-03-13 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.684417.1 [access date]
Sulfide concentrations from INSPIRE track 1. Dataset Description: <p>&nbsp;Sulfide concentrations from INSPIRE track 1.</p>
<p><strong>These data are associated with the paper: Picard A., et al., 2018</strong></p> Methods and Sampling: <p>Sulfide concentrations as a function of times in cultures of sulfate-reducing bacterium Desulfovibrio hydrothermalis AM13.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1344241 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1344241
completed
Peter Girguis
Harvard University
617-496-8328
Biological Laboratories, Room 3085 16 Divinity Ave
Cambridge
MA
02138-2020
USA
pgirguis@oeb.harvard.edu
pointOfContact
David Clarke
Harvard University
617-495-4140
School of Engineering and Applied Sciences 29 Oxford St., 303 Cruft Hall
Cambridge
MA
02138
clarke@seas.harvard.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
experiment_type
time
sulfide_culture1
sulfide_culture2
sulfide_culture3
sulfide_culture1_Fe
sulfide_culture2_Fe
sulfide_culture3_Fe
sulfide_culture4_Fe
sulfide_control1_slow
sulfide_control2_slow
culture_medium
Microscope
theme
None, User defined
experiment type
incubation time or duration
sulfide
sample description
featureType
BCO-DMO Standard Parameters
Raman Microscope
instrument
BCO-DMO Standard Instruments
Girguis_2013
service
Deployment Activity
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
INSPIRE Track 1: Microbial Sulfur Metabolism and its Potential for Transforming the Growth of Epitaxial Solar Cell Absorbers
https://osprey.bco-dmo.org/project/623700
INSPIRE Track 1: Microbial Sulfur Metabolism and its Potential for Transforming the Growth of Epitaxial Solar Cell Absorbers
<p>This INSPIRE award is partially funded by Biological Oceanography Program in Division of Ocean Sciences, in the Directorate of Geosciences; the Electronic and Photonic Materials Program in the Division of Materials Research, Directorate of Mathematical and Physical Sciences.</p>
<p>A simple idea motivates this project: By characterizing the mechanisms underlying pyrite film deposition by subsurface microbes living at hydrothermal vents, can approaches be developed to controllably grow high-purity pyrite films that could be used to produce low-cost photovoltaic solar cells? Recent in situ studies at hydrothermal vents have found "subsurface" microbes associated with the deposition of large crystalline metal sulfides (up to 1.1 millimeters), including iron pyrite. In laboratory incubations, vent microbes specifically deposited pyrite (FeS2), devoid of Zn, Cu and other metals that were abundant in the liquid media. Abiotic incubations did not exhibit this specificity. The investigators hypothesize that, in situ, microbes deposit pyrite via a number of potential processes, including a physiological process called extracellular electron transfer (EET), wherein microbes shuttle electrons to/from minerals. In situ, EET-enabled microbes may use conductive minerals to electrically access oxidants, and deposit pyrite on these surfaces. Vents are thus natural bioelectrochemical cells, which grow metal sulfides via microbial and abiotic electrochemical processes, though the details and mechanisms remain to be determined. This project is aimed at elucidating the mechanisms underlying microbial FeS2 pyrite bio-deposition, and assessing how microbes might be used to deposit epitaxial films for solar cells absorbers. FeS2 pyrite has been identified as prospective low cost solar absorbers because of their abundance, suitable band-gap (~0.95 eV) and high optical absorbance. Microbial pyrite film deposition at lower temperatures (<100 C) might offer a radically new, low cost approach to creating large area PV solar cells. Nothing is currently known about the mechanisms underlying microbial pyrite growth, though the large crystal sizes suggest epitaxial deposition is favored over re-nucleation implying that, once nucleated, epitaxial growth can occur. A series of experiments using natural vent microbial communities and isolates will be conducted to determine: A) environmental factors that influence bio-deposition; B) potential molecular mechanisms; C) the microstructural and electrical properties of these films; and D) whether bio-deposition by single species or consortia yields films of highest purity, size and homogeneity.</p>
<p>The project is both highly-integrated and transformative. It is relevant to our understanding of microbial sulfur cycling, as little is known about how microbes mediate crystalline pyrite formation and the degree to which this influences sulfur isotope geochemistry. Molecular studies will be used to interrogate relevant microbial metabolic processes and constrain the possible mechanisms of pyrite film growth, which is critical to advancing our ability to grow FeS2 films for device applications. Understanding the effects of substrate crystallography and electrical conductivity on the growth morphology will further inform our knowledge of microbial pyrite deposition. Notably, this research differ from existing biomimetic approaches. The studies are not focused on crystal growth via tethered peptides or synthetic extracellular matrices. Rather, they aim to advance our understanding of natural biodeposition, use the insights gained to grow pyrite materials and devices.</p>
<p> </p>
INSPIRE_Pyrite
largerWorkCitation
project
eng; USA
oceans
2013-01-01
2017-12-31
0
BCO-DMO catalogue of parameters from Sulfide concentrations as a function of time from INSPIRE track 1 collected from 2013 to 2017 (INSPIRE Pyrite project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/684621.rdf
Name: experiment_type
Units: unitless
Description: <p>Abiotic or biotic experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/684622.rdf
Name: time
Units: hours
Description: <p>Time in hours</p>
http://lod.bco-dmo.org/id/dataset-parameter/684623.rdf
Name: sulfide_culture1
Units: micromolar (mM)
Description: <p>Sulfide concentrations as a function of time in cultures of AM13 with trace Fe(II)</p>
http://lod.bco-dmo.org/id/dataset-parameter/684624.rdf
Name: sulfide_culture2
Units: micromolar (mM)
Description: <p>Sulfide concentrations as a function of time in cultures of AM13 with trace Fe(II)</p>
http://lod.bco-dmo.org/id/dataset-parameter/684625.rdf
Name: sulfide_culture3
Units: micromolar (mM)
Description: <p>Sulfide concentrations as a function of time in cultures of AM13 with trace Fe(II)</p>
http://lod.bco-dmo.org/id/dataset-parameter/684626.rdf
Name: sulfide_culture1_Fe
Units: micromolar (mM)
Description: <p>Sulfide concentrations as a function of time in AM13 cultures containing 4 mM Fe(II)</p>
http://lod.bco-dmo.org/id/dataset-parameter/684627.rdf
Name: sulfide_culture2_Fe
Units: micromolar (mM)
Description: <p>Sulfide concentrations as a function of time in AM13 cultures containing 4 mM Fe(II)</p>
http://lod.bco-dmo.org/id/dataset-parameter/684628.rdf
Name: sulfide_culture3_Fe
Units: micromolar (mM)
Description: <p>Sulfide concentrations as a function of time in AM13 cultures containing 4 mM Fe(II)</p>
http://lod.bco-dmo.org/id/dataset-parameter/684629.rdf
Name: sulfide_culture4_Fe
Units: micromolar (mM)
Description: <p>Sulfide concentrations as a function of time in AM13 cultures containing 4 mM Fe(II)</p>
http://lod.bco-dmo.org/id/dataset-parameter/684630.rdf
Name: sulfide_control1_slow
Units: micromolar (mM)
Description: <p>Sulfide added as a function of time in the medium</p>
http://lod.bco-dmo.org/id/dataset-parameter/684631.rdf
Name: sulfide_control2_slow
Units: micromolar (mM)
Description: <p>Sulfide added as a function of time in the medium</p>
http://lod.bco-dmo.org/id/dataset-parameter/684632.rdf
Name: culture_medium
Units: unitless
Description: <p>Description of culture medium</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
8154
https://darchive.mblwhoilibrary.org/bitstream/1912/24246/1/dataset-684417_sulfide-concentrations__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.684417.1
download
onLine
dataset
<p>Sulfide concentrations as a function of times in cultures of sulfate-reducing bacterium Desulfovibrio hydrothermalis AM13.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Data Processing Notes:</strong></p>
<p>- compiled multiple spreadsheets of sulfide concentrations into one table.<br />
- replaced blank cells with nd.<br />
- reformatted column names to comply with BCO-DMO standards.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Microscope
Microscope
PI Supplied Instrument Name: Microscope PI Supplied Instrument Description:Used to analyze cultures Instrument Name: Raman Microscope Instrument Short Name: Instrument Description: The Raman microscope is a laser-based microscopic device used to perform Raman spectroscopy. The Raman microscope begins with a standard optical microscope, and adds an excitation laser, laser rejection filters, a spectrometer or monochromator, and an optical sensitive detector such as a charge-coupled device (CCD), or photomultiplier tube, (PMT). One example is the XploRA confocal Raman microscope (information from the manufacturer).
Deployment: Girguis_2013
Girguis_2013
lab Harvard
laboratory
lab Harvard
laboratory