<div><p>Cultures of Emiliania huxleyi were obtained from the National Center for Marine Algae and Microbiota at Bigelow Laboratories (all CCMP strains), or from Dr. D. Iglesias-Rodriguez at UC Santa Barbara (strain NEZH) and maintained in the Strom laboratory at Shannon Point Marine Center.</p>
<p>Batch cultures were grown in 50-100 ml volumes of seawater (salinity = 30) amended with f/50 nutrients, at a temperature of 15 deg C and an irradiance of 140-300 umol photons m-2 s-1 on a 12L:12D light cycle. Replicate cultures (n=3) were subsampled for chemical measurements at cell densities of 1.4 to 2.7 x 10^5 cells ml-1 (DMSP) and 3.6 to 12.8 x 10^5 cells ml-1 (H2O2). Different chemical and size measurements reported for a given strain were made over the course of several separate experiments.</p>
<p>Dimethylsulfoniopropionate (DMSP) contained within E. huxleyi cells was measured using a Shimadzu GC-14A gas chromatograph and flame photometric detection, following the methods of Wolfe et al. (2002 J. Phycol. 38: 948-960). Cells were captured on 25 mm glass fiber filters (effective pore size 0.7 um) and placed into 3 ml 5N NaOH for hydrolysis. Method was standardized using ultrapure DMSP-Cl (standard range 0.625 to 50 nM; r2 ≥0.998).</p>
<p>Hydrogen peroxide (H2O2) released into the dissolved phase by E. huxleyi was measured using the Amplex Red – horseradish peroxidase method, using a kit from Molecular Probes (now part of Thermo Fisher Scientific) according to kit directions and to Suggett et al. (2008 J Phycol 44: 948-956). Fluorescent reaction product was quantified in a BioTek Synergy M plate reader (565 nm excitation, 585 nm emission). True reagent blanks were obtained by catalase treatment of E. huxleyi culture filtrate (50 U ml-1, 45 min, room temperature) following Shaked et al. (2010 Environ Sci Technol 44: 3238-3244). Method was standardized using ultrapure H2O2 (standard range 0.025 to 0.5 uM; r2 = 0.98)</p>
<p>E. huxleyi cell size was obtained by imaging live cells (n = 23-29) at 1000x magnification on a Leica DM5500 B microscope, and sizing them with associated image analysis software. Calcification (i.e. whether a strain harbored coccoliths) was also confirmed during microscopy. Note that the sample of strain CCMP3266 used for size measurement comprised a mixture of calcifying and non-calcifying cells.</p></div>
Cell size and chemical characteristics of five strains of coccolithophore Emiliania huxleyi.
<div><p>Cell size and chemical characteristics (H2O2 production, DMSP content) of five strains of coccolithophore <em>Emiliania huxleyi</em>, grown in laboratory culture</p></div>
Emiliania size and chemical characteristics
<div><p><strong>BCO-DMO Data Processing Notes:</strong></p>
<p>-Data compiled into one table from multiple spreadsheets<br />
-Replaced all blank cells with "nd"</p></div>
684883
Emiliania size and chemical characteristics
2017-03-20T12:51:34-04:00
2017-03-20T12:51:34-04:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:684883
Cell size and chemical characteristics of five strains of coccolithophore Emiliania huxleyi (Protist signaling project)
Cell size and chemical characteristics of five strains of coccolithophore Emiliania huxleyi (Protist signaling project)
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Strom, S. (2017) Cell size and chemical characteristics of five strains of coccolithophore Emiliania huxleyi (Protist signaling project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2017-03-20 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.684883.1 [access date]
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2017-03-20
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