<div><p>All experiments were approved by the University of Hawaii’s Institutional Animal Care & Use Committee (IACUC) under protocol #1045. For determination of growth, larval <em>Amphiprion </em><em>ocellaris</em> were euthanized using a solution of 0.06 g/ml Ethyl 3-aminobenzoate methanesulfonate salt (MS222) (Sigma-Aldrich Inc., Saint Louis, MO, USA; catalog no. A5040-25G), preserved in a solution of 5% formalin in seawater, and measured for total length and jaw size within one week of fixation.</p>
<p>Behavioral observations were made of predator-prey interactions between larval fish and copepod prey under conditions that were similar to the feeding conditions in the rearing tank in terms of prey numbers and fish density. For each trial, 10 <em>A. </em><em>ocellaris</em> larvae were transferred from the rearing tank into the observation chamber (18 x 18 x 10 cm aquarium made of Plexiglas) filled with 3 L of filtered seawater (Whatman filters, GF/C). The fish larvae were allowed to acclimate for 15 minutes, before adding the 3000 prey. Each experimental trial lasted for 60 minutes and prey densities declined by 25% or less during this time. During the trial, a CCTV video camera (Panasonic Corporation, Kadoma, Osaka, Japan; model WV-BP310) equipped with a Nikkor 50 mm lens (Nikon Corporation, Shinjuku, Tokyo, Japan; model 1433) recorded continuously and stored the recording on a digital high definition videocassette recorder (Sony Corporation, Minato, Tokyo, Japan; model GV-HD700). The camera lens was positioned 0.3 m from the observation chamber and the lens was focused in a plane at the center such that the field of view was 4 cm2. The container was uniformly illuminated from above with one 20-watt fluorescent light providing 1,900 lumens of light. </p>
<p>Two separate experimental series were completed: the first one focused on three larval fish ages (1, 3, and 10 dph), and offered a single prey type per experiment (nauplii, copepodites or adults). The second one recorded predatory-prey interactions for larval ages 1 to 14 dph and mixed prey fields (nauplii + copepodites + adults). Capture success was determined from video recordings of predatory interactions between larval clownfish and three developmental stage groups of the copepod <em>Parvocalanus </em><em>crassirostris</em>.</p></div>
Larval clownfish (Amphiprion ocellaris) predatory success during first 14 days post-hatch
<div><p>This dataset includes the predatory success of larval clownfish (<em>Amphiprion </em><em>ocellaris</em>) between 1 and 14 days post-hatch. They were presented with nauplii, copepodites, and adults of the copepod <em>Parvocalanus </em><em>crassirostris</em>.</p>
<p><strong>Related Reference:</strong><br />
Jackson, J. M. and Lenz, P. H. (2016). Predator-prey interactions in the plankton: larval fish feeding on evasive copepods. Scientific Reports, 6, 33585; doi: <a href="http://www.nature.com/articles/srep33585" target="_blank">10.1038/srep33585</a>.</p></div>
Larval clownfish predatory success
<div><p>For the data analysis, videos were reviewed, larval predatory strikes identified and scored as to outcome (successful/unsuccessful). In a second analysis for experiment II, predatory strikes were scored for the targeted prey type to determine selectivity. For approximately 15% of strikes, resolution, clarity or contrast was insufficient to accurately determine prey type and these were excluded from the final dataset.</p>
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions</p></div>
686907
Larval clownfish predatory success
2017-04-04T15:14:20-04:00
2017-04-04T15:14:20-04:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:686907
Larval clownfish (Amphiprion ocellaris) predatory success during first 14 days post-hatch from Lenz lab in the University of Hawaii at Manoa lab from 2012-2015 (PreyEscape project)
false
Lenz, P., Hartline, D. K. (2017) Larval clownfish (Amphiprion ocellaris) predatory success during first 14 days post-hatch from Lenz lab in the University of Hawaii at Manoa lab from 2012-2015 (PreyEscape project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2017-04-04 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/686907 [access date]
true
false
2017-04-04
HTML
https://www.bco-dmo.org/dataset/686907
text/html
Datapackage.json
Frictionless Data Package
https://www.bco-dmo.org/dataset/686907/datapackage.json
application/vnd.datapackage+json
PDF
https://www.bco-dmo.org/dataset/686907/Dataset_description.pdf
application/pdf
JSON-LD
https://www.bco-dmo.org/dataset/686907.json
application/ld+json
Turtle
https://www.bco-dmo.org/dataset/686907.ttl
text/turtle
RDF/XML
https://www.bco-dmo.org/dataset/686907.rdf
application/rdf+xml
ISO 19115-2 (NOAA Profile)
https://www.bco-dmo.org/dataset/686907/iso
application/xml
http://www.isotc211.org/2005/gmd-noaa
Dublin Core
https://www.bco-dmo.org/dataset/686907/dublin-core
application/xml
http://purl.org/dc/elements/1.1/
686907
http://lod.bco-dmo.org/id/dataset/686907
OSPREY
http://www.opengis.net/def/crs/OGC/1.3/CRS84
<http://www.opengis.net/def/crs/OGC/1.3/CRS84> POINT (-157.8197 21.3)
21.3
-157.8197
21.300000000000
-157.819700000000