http://lod.bco-dmo.org/id/dataset/692753
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2017-04-28
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Zooplankton community abundances from meter net tows on multiple cruises on RV/Savannah in the South Atlantic Bight, Mid-Continental Shelf from 2015-2017
2020-04-08
publication
2020-04-08
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-04-09
publication
https://doi.org/10.26008/1912/bco-dmo.692753.3
Marc E. Frischer
Skidaway Institute of Oceanography
principalInvestigator
Deidre M. Gibson
Hampton University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Frischer, M., Gibson, D. (2020) Zooplankton community abundances from meter net tows on multiple cruises on RV/Savannah in the South Atlantic Bight, Mid-Continental Shelf from 2015-2017. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 3) Version Date 2020-04-08 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.692753.3 [access date]
Zooplankton abundances Dataset Description: <p>This dataset includes zooplankton counts and abundances from meter net tows on multiple cruises on RV/Savannah in the South Atlantic Bight, Mid-Continental Shelf from August 2015 to December 2017.</p>
<p>Latitude and longitude data are&nbsp;included in version 3.</p> Methods and Sampling: <p>Zooplankton was collected from 31°N to 29°N aboard the R/V Savannah at the 25m and 40m isobaths using a 1M (mouth opening) 5M length 200 µm mesh cone plankton net equipped with a filtering (202 µm mesh) cod-end.&nbsp; The mouth of the net was mounted in a swivel towing frame that allowed the opening of the net to track the water current. A mechanical flow meter (General Oceanics Part# 2030RC) was mounted in the center of the net. <a href="http://dmoserv3.bco-dmo.org/data_docs/Doliolid_Diet/Frischer_Flowmeter_Zooplankton_tows.pdf" target="_blank">Flowmeter measurements for 2015 cruises (pdf)</a> The net was deployed from a gently drifting ship and the entire water column was sampled by lowering the net to near the bottom and retrieving it (oblique tow).&nbsp; Net speed was maintained at approximately 15 meters per minute. Once retrieved the contents of the net were collected on a 200 µm sieve and preserved in 60% non-denatured ethanol.</p>
<p>Samples were split twice to create four subsamples using a Folsom plankton splitter.</p>
<p>One of the subsamples was diluted to a known volume and stirred gently. Dilutions were adjusted depending on the density of zooplankton so that sufficient numbers of species were present in each sample to obtain a reliable estimate of the major taxonomic groups in each sample. Replicate aliquots from the subsample were counted under a dissecting microscope using a Bogorov counting chamber. The volume of sample counted was adjusted based on the density of zooplankton present but was generally 5 ml. A Stempel pipette was used to dispense the counted volume.</p>
<p>In the case where the identity was uncertain, representative examples of the unidentified zooplankton were identified by DNA barcoding.&nbsp; DNA extractions were performed using the DNeasy Blood &amp; Tissue Kit (Qiagen) according to manufacturer’s protocol except that samples were macerated and homogenized with Kimble/Kontes Cordless Motor, the proteinase K incubation was extended to ~24 hours (overnight) and the volume of elution buffer AE varied depending on sample size (60ul for most). For DNA quantitation and quality testing, one (1) ul of DNA was used to measure the 260/280 ratio using the Thermo Scientific™&nbsp; NanoDrop Lite Spectrophotometer.&nbsp; The presence of high molecular weight DNA in each extract was visually confirmed by agarose (1%) gel electrophoresis.&nbsp; Good quality DNA extractions, inferred by 260/280 ratio and electrophoresis, were selected for amplification by Polymerase Chain Reaction (PCR) of a fragment of the mitochondrial cytochrome oxidase subunit I (mtCOI) gene.&nbsp; A 708 base-pair mtCOI gene fragment was amplified using the consensus primer pairs LCO-1490 (5’-GGTCAACAAATCATAAAGATATTGG-3’) and HCO-2198 (5’-TAAACTTCAGGGTGACCAAAAAATCA-3’) (Folmer et al. 1994).&nbsp; Amplification was facilitated using a Bio-Rad T100Thermocycler with GoTaq Hot Start Green Master Mix. Cycling conditions were: 10 min at 95 °C; 35 cycles of 30 sec at 95 °C, 1 min at 47 °C and 1 min at 72 °C; and 10 min at 72 °C. PCR product quality and quantity was assessed by 2% agarose gel electrophoresis and subsequently sequenced by Eurofins Genomics. DNA sequences obtained were compared to a reference database library using the Basic Local Alignment Search Tool (BLAST) (Altschul et al. 1990) available at the National Center for Biotechnology Information (NCBI) and Bold Systems Identification engine for CO1 gene.&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1459293 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1459293
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1459510 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1459510
completed
Marc E. Frischer
Skidaway Institute of Oceanography
(912) 598-2308
10 Ocean Science Circle
Savannah
GA
31411
USA
marc.frischer@skio.usg.edu
pointOfContact
Deidre M. Gibson
Hampton University
757-727-5883
100 E. Queen Street
Hampton
VA
23668
USA
deidre.gibson@hamptonu.edu
pointOfContact
asNeeded
Dataset Version: 3
Unknown
cruise_id
date
time_tow
lat_decdeg
lon_decdeg
station_depth_water
mesh
vol_filt
cast
replicate
splits
taxon_1
taxon_2
dilution_factor
count_aliquot_1
count_aliquot_2
count_mean
count_sample
abundance_m3
ISO_DateTime_UTC
mechanical flow meter (General Oceanics Part# 2030RC)
meter net
Thermo Scientific™ NanoDrop Lite Spectrophotometer
Bio-Rad T100Thermocycler
theme
None, User defined
Cruise identfier
date
time of day
latitude
longitude
depth_w
net mesh size
volume of water filtered
cast
tow
No BCO-DMO term
taxon
count
abundance
ISO_DateTime_UTC
featureType
BCO-DMO Standard Parameters
Automated DNA Sequencer
Flow Meter
Folsom Plankton Splitter
Meter Net
Spectrophotometer
Thermal Cycler
instrument
BCO-DMO Standard Instruments
SAV-15-19
SAV-15-31
SAV-16-04
SAV-16-06
SAV-16-08
SAV-16-11
SAV-16-15
SAV-17-16
SAV-17-19
SAV-17-22
SAV-17-23
SAV-17-25
SAV-16-05
SAV-16-07
SAV-16-09
SAV-16-10
SAV-16-12
SAV-16-13
SAV-16-14
SAV-17-17
SAV-17-18
SAV-17-20
SAV-17-21
SAV-17-24
service
Deployment Activity
South Atlantic Bight, Mid-Continental Shelf
South Atlantic Bight off the coast of Georgia
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
The cryptic diet of the globally significant pelagic tunicate Dolioletta gegenbauri (Uljanin, 1884.)
https://osprey.bco-dmo.org/project/641283
The cryptic diet of the globally significant pelagic tunicate Dolioletta gegenbauri (Uljanin, 1884.)
<p><em>Project description from NSF award abstract:</em><br />
Gelatinous (soft-bodied) zooplankton can play a crucial role in food webs and in cycling of materials in the world's oceans, and it has been suggested that they may become even more important in the future. However, because they are so difficult to study, gelatinous species remain poorly understood. This is especially true for smaller filter feeding gelatinous animals such as pelagic tunicates (salps, larvaceans, and doliolids). For example, it remains unclear what and how much these abundant filter feeders eat in nature and who eats them. This project will address this large and significant knowledge gap by using a combination of new and traditional methods to investigate the diet of the gelatinous pelagic tunicate <em>Dolioletti gegenbauri</em>, a species common on productive continental shelves such as the South Atlantic Bight. This project will also help train the next generation of ocean scientists to be competent in classical biology, modern molecular biology, and ecosystem modeling. Training will also focus on increasing representation of African Americans in the future science, technology, engineering, and math (STEM) workforce.</p>
<p>This study will provide the first quantitative estimates of the in situ diet of a key continental shelf gelatinous zooplankton species, the doliolid <em>Dolioletta gegenbauri</em>. Large blooms of doliolids have the potential to control the trophic structure of shelf pelagic ecosystems by shunting primary production to the microbial food web and by limiting copepod production via the consumption of their eggs. The long-term objective is to understand the ecological role and significance of doliolids in continental shelf pelagic ecosystems, specifically the underlying processes that lead to their high level of spatial and temporal patchiness. The basic questions to be addressed here include: What do doliolids eat, in situ, at different life stages? Are early life stages of larger metazoans important components of their diets? Do doliolids act as trophic cascade agents promoting primary production and phytoplankton diversity? Because of methodological challenges, there have not yet been definitive studies addressing these fundamental questions. In this project, the investigators will conduct field-based studies that will combine state-of-the art molecular techniques with more traditional methods in zooplankton ecology to answer questions about trophic interactions. Monthly oceanographic expeditions in the South Atlantic Bight will allow the research team to study wild doliolids at different time points in their life cycle and under different plankton bloom conditions. Application of recently developed molecular diagnostic assays will enable the quantitative description of the diversity and quantity of prey consumed, unbiased by experimental manipulation. Additional experimental and theoretical modeling will allow the investigators to link these data with larger ecological significance and scale.</p>
Doliolid Diet
largerWorkCitation
project
eng; USA
biota
oceans
South Atlantic Bight, Mid-Continental Shelf; South Atlantic Bight off the coast of Georgia
-80.642
-80.2082
29.9447
31
2015-08-04
2017-12-05
South Atlantic Bight
0
BCO-DMO catalogue of parameters from Zooplankton community abundances from meter net tows on multiple cruises on RV/Savannah in the South Atlantic Bight, Mid-Continental Shelf from 2015-2017
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/692771.rdf
Name: cruise_id
Units: unitless
Description: <p>cruise identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/692773.rdf
Name: date
Units: unitless
Description: <p>local date</p>
http://lod.bco-dmo.org/id/dataset-parameter/692774.rdf
Name: time_tow
Units: unitless
Description: <p>local time of the replicate tow</p>
http://lod.bco-dmo.org/id/dataset-parameter/692775.rdf
Name: lat_decdeg
Units: decimal degrees
Description: <p>latitude; north is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/692776.rdf
Name: lon_decdeg
Units: decimal degrees
Description: <p>longitude; east is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/692777.rdf
Name: station_depth_water
Units: meters
Description: <p>sampling depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/692778.rdf
Name: mesh
Units: microns
Description: <p>mesh opening size</p>
http://lod.bco-dmo.org/id/dataset-parameter/692779.rdf
Name: vol_filt
Units: cubic meters (m^3)
Description: <p>volume of water filtered by net for a replicate tow</p>
http://lod.bco-dmo.org/id/dataset-parameter/692780.rdf
Name: cast
Units: unitless
Description: <p>cast number</p>
http://lod.bco-dmo.org/id/dataset-parameter/692781.rdf
Name: replicate
Units: unitless
Description: <p>replicate tow number; two tows were taken at each station</p>
http://lod.bco-dmo.org/id/dataset-parameter/692782.rdf
Name: splits
Units: splits
Description: <p>number of times the sample was split; fraction of sample = 1/n^2 so splits=2 means 1/4 of sample was processed. Except for splits = 0, this means the entire sample was used for the taxonomic count.</p>
http://lod.bco-dmo.org/id/dataset-parameter/692783.rdf
Name: taxon_1
Units: unitless
Description: <p>taxon 1: holoplankton or meroplankton</p>
http://lod.bco-dmo.org/id/dataset-parameter/692784.rdf
Name: taxon_2
Units: unitless
Description: <p>most specific taxonomic group identified</p>
http://lod.bco-dmo.org/id/dataset-parameter/692785.rdf
Name: dilution_factor
Units: unitless
Description: <p>sample dilution factor</p>
http://lod.bco-dmo.org/id/dataset-parameter/692786.rdf
Name: count_aliquot_1
Units: number of individuals per sample
Description: <p>first aliquot raw count from replicate sample split</p>
http://lod.bco-dmo.org/id/dataset-parameter/692787.rdf
Name: count_aliquot_2
Units: number of individuals per sample
Description: <p>second aliquot raw count from replicate sample split</p>
http://lod.bco-dmo.org/id/dataset-parameter/692788.rdf
Name: count_mean
Units: number of individuals per sample
Description: <p>mean raw count</p>
http://lod.bco-dmo.org/id/dataset-parameter/692789.rdf
Name: count_sample
Units: number of individuals per sample
Description: <p>calculated average abundance of individuals per sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/692790.rdf
Name: abundance_m3
Units: individuals per cubic meter of water
Description: <p>estimated abundance</p>
http://lod.bco-dmo.org/id/dataset-parameter/805204.rdf
Name: ISO_DateTime_UTC
Units: yyyy-MM-dd'T'HH:mm:ss'Z'
Description: <p>UTC date and time of tow in ISO format: yyyy-mm-ddTHH:MM:SSZ</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
774003
https://darchive.mblwhoilibrary.org/bitstream/1912/25622/1/dataset-692753_doliolid-diet-zooplankton-abundance__v3.tsv
download
https://doi.org/10.26008/1912/bco-dmo.692753.3
download
onLine
dataset
<p>Zooplankton was collected from 31°N to 29°N aboard the R/V Savannah at the 25m and 40m isobaths using a 1M (mouth opening) 5M length 200 µm mesh cone plankton net equipped with a filtering (202 µm mesh) cod-end.&nbsp; The mouth of the net was mounted in a swivel towing frame that allowed the opening of the net to track the water current. A mechanical flow meter (General Oceanics Part# 2030RC) was mounted in the center of the net. <a href="http://dmoserv3.bco-dmo.org/data_docs/Doliolid_Diet/Frischer_Flowmeter_Zooplankton_tows.pdf" target="_blank">Flowmeter measurements for 2015 cruises (pdf)</a> The net was deployed from a gently drifting ship and the entire water column was sampled by lowering the net to near the bottom and retrieving it (oblique tow).&nbsp; Net speed was maintained at approximately 15 meters per minute. Once retrieved the contents of the net were collected on a 200 µm sieve and preserved in 60% non-denatured ethanol.</p>
<p>Samples were split twice to create four subsamples using a Folsom plankton splitter.</p>
<p>One of the subsamples was diluted to a known volume and stirred gently. Dilutions were adjusted depending on the density of zooplankton so that sufficient numbers of species were present in each sample to obtain a reliable estimate of the major taxonomic groups in each sample. Replicate aliquots from the subsample were counted under a dissecting microscope using a Bogorov counting chamber. The volume of sample counted was adjusted based on the density of zooplankton present but was generally 5 ml. A Stempel pipette was used to dispense the counted volume.</p>
<p>In the case where the identity was uncertain, representative examples of the unidentified zooplankton were identified by DNA barcoding.&nbsp; DNA extractions were performed using the DNeasy Blood &amp; Tissue Kit (Qiagen) according to manufacturer’s protocol except that samples were macerated and homogenized with Kimble/Kontes Cordless Motor, the proteinase K incubation was extended to ~24 hours (overnight) and the volume of elution buffer AE varied depending on sample size (60ul for most). For DNA quantitation and quality testing, one (1) ul of DNA was used to measure the 260/280 ratio using the Thermo Scientific™&nbsp; NanoDrop Lite Spectrophotometer.&nbsp; The presence of high molecular weight DNA in each extract was visually confirmed by agarose (1%) gel electrophoresis.&nbsp; Good quality DNA extractions, inferred by 260/280 ratio and electrophoresis, were selected for amplification by Polymerase Chain Reaction (PCR) of a fragment of the mitochondrial cytochrome oxidase subunit I (mtCOI) gene.&nbsp; A 708 base-pair mtCOI gene fragment was amplified using the consensus primer pairs LCO-1490 (5’-GGTCAACAAATCATAAAGATATTGG-3’) and HCO-2198 (5’-TAAACTTCAGGGTGACCAAAAAATCA-3’) (Folmer et al. 1994).&nbsp; Amplification was facilitated using a Bio-Rad T100Thermocycler with GoTaq Hot Start Green Master Mix. Cycling conditions were: 10 min at 95 °C; 35 cycles of 30 sec at 95 °C, 1 min at 47 °C and 1 min at 72 °C; and 10 min at 72 °C. PCR product quality and quantity was assessed by 2% agarose gel electrophoresis and subsequently sequenced by Eurofins Genomics. DNA sequences obtained were compared to a reference database library using the Basic Local Alignment Search Tool (BLAST) (Altschul et al. 1990) available at the National Center for Biotechnology Information (NCBI) and Bold Systems Identification engine for CO1 gene.&nbsp;</p>
Specified by the Principal Investigator(s)
<p>The abundance of each zooplankton group was estimated accounting for all sample dilution and extrapolated based on the calculated initial net sampling volume.&nbsp; No further data processing was involved.&nbsp; All data was reviewed by at least one other expert.&nbsp; If outliers were identified all calculations were confirmed and in cases where errors were not obvious the sample was re-counted.</p>
<p><strong>BCO-DMO Processing Notes:<br />
Version 1 [</strong><strong>2017-04-28]: (2015 data)</strong><br />
- added&nbsp;conventional&nbsp;header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- blank values were replaced with no data value 'nd'<br />
- added columns for cruise id, station, date, time, lat, lon, volume filtered, cast, replicate, splits, taxon_1<br />
<strong>Version 2 [2020-02-24] (2015-2017 data)</strong><br />
<span style="background-color:rgb(255, 255, 255); color:rgb(51, 51, 51); font-family:sans-serif,arial,verdana,trebuchet ms; font-size:13px">- added&nbsp;conventional&nbsp;header with dataset name, PI name, version date</span><br />
<span style="background-color:rgb(255, 255, 255); color:rgb(51, 51, 51); font-family:sans-serif,arial,verdana,trebuchet ms; font-size:13px">- modified parameter names to conform with BCO-DMO naming conventions</span><br />
<span style="background-color:rgb(255, 255, 255); color:rgb(51, 51, 51); font-family:sans-serif,arial,verdana,trebuchet ms; font-size:13px">- blank and&nbsp;ND values were replaced with&nbsp;'nd'<br />
- date was converted to yyyy-mm-dd and ISO_DateTime_UTC was added<br />
- "Jekyll&nbsp; Is" was replaced with "Jekyll Is" (one space between words)<br />
- replaced blanks with zeroes in 'splits'&nbsp;column</span><br />
<strong>Version 3 [2020-04-08] (2015-2017 data; includes lats/lons)</strong><br />
- added&nbsp;conventional&nbsp;header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- blanks, NO DATA, and&nbsp;ND values were replaced with&nbsp;'nd'<br />
- date was converted to yyyy-mm-dd and ISO_DateTime_UTC was added<br />
- latitude and longitude were converted from degrees, minutes, seconds to decimal degrees.<br />
&nbsp;</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: Instrument Name: Automated DNA Sequencer Instrument Short Name:Automated Sequencer Instrument Description: A DNA sequencer is an instrument that determines the order of deoxynucleotides in deoxyribonucleic acid sequences.
mechanical flow meter (General Oceanics Part# 2030RC)
mechanical flow meter (General Oceanics Part# 2030RC)
PI Supplied Instrument Name: mechanical flow meter (General Oceanics Part# 2030RC) PI Supplied Instrument Description:Used to measure volume of water passing through the plankton net. Instrument Name: Flow Meter Instrument Short Name:Flow Meter Instrument Description: General term for a sensor that quantifies the rate at which fluids (e.g. water or air) pass through sensor packages, instruments, or sampling devices. A flow meter may be mechanical, optical, electromagnetic, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/388/
PI Supplied Instrument Name: PI Supplied Instrument Description:Used to split plankton samples into equivalent subsamples. Instrument Name: Folsom Plankton Splitter Instrument Short Name:Folsom Splitter Instrument Description: A device for sub-sampling of plankton and ichthyoplankton samples by splitting, developed by Dr. Folsom of the Scripps Institute of Oceanography. Ideally suited for splitting plankton samples with minimal debris. A measured volume of plankton sample is placed in the undivided section of the drum. This is rotated 120 degrees to divide the stirred sample with a separating blade. Standard Methods suggests splitting until a subsample of 200-500 individuals is obtained.
meter net
meter net
PI Supplied Instrument Name: meter net PI Supplied Instrument Description:1M (mouth opening) 5M length 200 µm mesh cone plankton net equipped with a filtering (202 µm mesh) cod-end. The mouth of the net was mounted in a swivel towing frame that allowed the opening of the net to track the water current. A mechanical flow meter (General Oceanics Part# 2030RC) was mounted in the center of the net. Instrument Name: Meter Net Instrument Short Name:Meter Net Instrument Description: A meter net is a plankton net with a one meter diameter opening and a mesh size of .333 mm, towed horizontally, obliquely or vertically, also known as a Ring Net. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/22/
Thermo Scientific™ NanoDrop Lite Spectrophotometer
Thermo Scientific™ NanoDrop Lite Spectrophotometer
PI Supplied Instrument Name: Thermo Scientific™ NanoDrop Lite Spectrophotometer PI Supplied Instrument Description:Used for DNA quantitation and quality testing. Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/
Bio-Rad T100Thermocycler
Bio-Rad T100Thermocycler
PI Supplied Instrument Name: Bio-Rad T100Thermocycler Instrument Name: Thermal Cycler Instrument Short Name:Thermal Cycler Instrument Description: A thermal cycler or "thermocycler" is a general term for a type of laboratory apparatus, commonly used for performing polymerase chain reaction (PCR), that is capable of repeatedly altering and maintaining specific temperatures for defined periods of time. The device has a thermal block with holes where tubes with the PCR reaction mixtures can be inserted. The cycler then raises and lowers the temperature of the block in discrete, pre-programmed steps. They can also be used to facilitate other temperature-sensitive reactions, including restriction enzyme digestion or rapid diagnostics.
(adapted from http://serc.carleton.edu/microbelife/research_methods/genomics/pcr.html)
Cruise: SAV-15-19
SAV-15-19
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-15-19
Marc E. Frischer
Skidaway Institute of Oceanography
Cruise: SAV-15-31
SAV-15-31
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-15-31
Marc E. Frischer
Skidaway Institute of Oceanography
Cruise: SAV-16-04
SAV-16-04
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-16-04
Marc E. Frischer
Skidaway Institute of Oceanography
Cruise: SAV-16-06
SAV-16-06
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-16-06
Peter Lee
College of Charleston - Hollings Marine Lab
Cruise: SAV-16-08
SAV-16-08
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-16-08
Marc E. Frischer
Skidaway Institute of Oceanography
Cruise: SAV-16-11
SAV-16-11
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-16-11
Marc E. Frischer
Skidaway Institute of Oceanography
Cruise: SAV-16-15
SAV-16-15
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-16-15
Marc E. Frischer
Skidaway Institute of Oceanography
Cruise: SAV-17-16
SAV-17-16
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-17-16
James T. Hollibaugh
University of Georgia
Cruise: SAV-17-19
SAV-17-19
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-17-19
Marc E. Frischer
Skidaway Institute of Oceanography
Cruise: SAV-17-22
SAV-17-22
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-17-22
Marc E. Frischer
Skidaway Institute of Oceanography
Cruise: SAV-17-23
SAV-17-23
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-17-23
Marc E. Frischer
Skidaway Institute of Oceanography
Cruise: SAV-17-25
SAV-17-25
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-17-25
Marc E. Frischer
Skidaway Institute of Oceanography
Cruise: SAV-16-05
SAV-16-05
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-16-05
Elizabeth Harvey
Skidaway Institute of Oceanography
Cruise: SAV-16-07
SAV-16-07
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-16-07
Martial Taillefert
Georgia Institute of Technology
Cruise: SAV-16-09
SAV-16-09
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-16-09
Steven Hall
University of North Carolina - Wilmington
Cruise: SAV-16-10
SAV-16-10
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-16-10
Gustav-Adolf Paffenhofer
Skidaway Institute of Oceanography
Cruise: SAV-16-12
SAV-16-12
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-16-12
Renato Castelao
Skidaway Institute of Oceanography
Cruise: SAV-16-13
SAV-16-13
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-16-13
Leslie Sautter
College of Charleston
Cruise: SAV-16-14
SAV-16-14
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-16-14
Warren Mitchell
Cruise: SAV-17-17
SAV-17-17
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-17-17
Zeb Schobernd
Cruise: SAV-17-18
SAV-17-18
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-17-18
Nathan Bacheler
NOAA Southeast Fisheries Science Center
Cruise: SAV-17-20
SAV-17-20
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-17-20
Marc E. Frischer
Skidaway Institute of Oceanography
Cruise: SAV-17-21
SAV-17-21
R/V Savannah
Community Standard Description
R/V Savannah
vessel
SAV-17-21
Elizabeth Harvey
Cruise: SAV-17-24
SAV-17-24
R/V Savannah
Community Standard Description
R/V Savannah
vessel
R/V Savannah
Community Standard Description
R/V Savannah
vessel