http://lod.bco-dmo.org/id/dataset/699469
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2017-05-04
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Record of d2H of dinosterol variability in down core lake sediments from Clear Lake, Palau
2017-05-04
publication
2017-05-04
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-08-02
publication
https://doi.org/10.1575/1912/bco-dmo.699469.1
Julian P. Sachs
University of Washington
principalInvestigator
Julie N. Richey
University of Washington
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Sachs, J., Richey, J. (2017) Record of d2H of dinosterol variability in down core lake sediments from Clear Lake, Palau. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2017-05-04 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.699469.1 [access date]
Record of d2H of dinosterol variability in down core lake sediments Dataset Description: <p>Record of d<sup>2</sup>H of dinosterol variability in down core lake sediments from Clear Lake, Palau.</p>
<p>These data are published in the following journal article:<br />
Richey, J. N., &amp; Sachs, J. P. (2016). Precipitation changes in the western tropical Pacific over the past millennium. Geology, 44(8), 671–674. doi:<a href="http://dx.doi.org/10.1130/g37822.1" target="_blank">10.1130/g37822.1</a>&nbsp;</p> Methods and Sampling: <p><strong>Sampling and analytical procedures:</strong><br />
Clear Lake sediments were extracted using a Dionex Accelerated Solvent Extractor (ASE 200), and the resulting total lipid extract was separated into neutral and polar fractions using column chromatography with aminopropyl gel as the stationary phase. The neutral fraction was then separated into hydrocarbon, wax ester, sterol and polar fractions via a silica gel column chromatography. Dinosterol was isolated then from the sterol fraction via reverse phase (RP)- high-performance liquid chromatography (HPLC)
.</p>
<p><strong>Instruments:</strong><br />
Dinosterol was isolated from the sterol fraction via reverse phase (RP)- high performance liquid chromatography (HPLC). An Agilent 1100 HPLC with an integrated autoinjector, quaternary pump, and fraction collector was coupled to an Agilent 1100 LC/MSD SL mass spectrometer with a multimode source that was operated in positive atmospheric pressure chemical ionization (APCI+) mode. The HPLC method used is outlined in Nelson and Sachs (2013).&nbsp;Subsequently to HPLC separation the fraction containing dinosterol was analyzed via GC-MSD to verify sufficient baseline separation. Adjacent HPLC fractions were also analyzed to ensure that no dinosterol eluted into those fractions.</p>
<p>After the dinosterol was sufficiently isolated from co-eluting compounds, the sample was injected onto a GC-irms for determination of the d<sup>2</sup>H of dinosterol.&nbsp; Hydrogen isotope determinations were made using a Finnigan Delta V Plus Isotope Ratio Mass Spectrometer (irMS) coupled to a Thermo Trace GC Ultra with a Varian VF-17ms FactorFour capillary column (60 m x 0.32 mm x 0.25 m) and a pyrolysis reactor. Samples were injected into a split/splitless inlet in splitless mode at 310 C. The oven temperature was ramped from 100 C to 220 C at a rate of 20 C/min, then at 2 C /min up to 325 C where it was held for 17 min. The carrier gas, He, was held constant at 2.6 mL/min. The pyroloysis reactor was maintained at 1400 C. Isotope values, expressed as D values, were calculated in Isodat software relative to VSMOW using a co-injection standard containing nC<sub>28</sub> nC<sub>32</sub>, nC<sub>40</sub>, and nC<sub>44</sub> of known ∂<sup>2</sup>H values (obtained from Arndt Schimmelmann, Indiana University, Bloomington, IN, USA). The measured isotope values of dinosterol were corrected for the addition of hydrogen atoms (of known D value) that occurred during acetylation. Each sample was analyzed in at least duplicate, and error bars represent standard deviations of replicate measurements.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1241247 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1241247
completed
Julian P. Sachs
University of Washington
206-221-5630
School of Oceanography Box 357940
Seattle
WA
357940
United States
jsachs@washington.edu
pointOfContact
Julie N. Richey
University of Washington
727-502-8123
600 4th Street South
St. Petersburg
FL
33701
USA
jrichey@usgs.gov
pointOfContact
asNeeded
Dataset Version: 1
Unknown
location
lat
lon
core_depth
age
dDdino
dDdino_stdev
high-performance liquid chromatography
Finnigan Delta V Plus Isotope Ratio Mass Spectrometer (irMS)
Agilent 1100 LC/MSD SL mass spectrometer
theme
None, User defined
site
latitude
longitude
depth below seafloor
age
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
High-Performance Liquid Chromatograph
Isotope-ratio Mass Spectrometer
Mass Spectrometer
instrument
BCO-DMO Standard Instruments
Palau_lakes
service
Deployment Activity
NW Pacific
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Dimensions of Biodiversity
http://www.nsf.gov/funding/pgm_summ.jsp?pims_id=503446
Dimensions of Biodiversity
(adapted from the NSF Synopsis of Program)
Dimensions of Biodiversity is a program solicitation from the NSF Directorate for Biological Sciences. FY 2010 was year one of the program. [MORE from NSF]
The NSF Dimensions of Biodiversity program seeks to characterize biodiversity on Earth by using integrative, innovative approaches to fill rapidly the most substantial gaps in our understanding. The program will take a broad view of biodiversity, and in its initial phase will focus on the integration of genetic, taxonomic, and functional dimensions of biodiversity. Project investigators are encouraged to integrate these three dimensions to understand the interactions and feedbacks among them. While this focus complements several core NSF programs, it differs by requiring that multiple dimensions of biodiversity be addressed simultaneously, to understand the roles of biodiversity in critical ecological and evolutionary processes.
Dimensions of Biodiversity
largerWorkCitation
program
Do Parallel Patterns Arise from Parallel Processes?
http://marinelakes.ucmerced.edu/
Do Parallel Patterns Arise from Parallel Processes?
<p>This project will survey the taxonomic, genetic, and functional diversity of the organisms found in marine lakes, and investigate the processes that cause gains and losses in this biodiversity. Marine lakes formed as melting ice sheets raised sea level after the last glacial maximum and flooded hundreds of inland valleys around the world. Inoculated with marine life from the surrounding sea and then isolated to varying degrees for the next 6,000 to 15,000 years, these marine lakes provide multiple, independent examples of how environments and interactions between species can drive extinction and speciation. Researchers will survey the microbes, algae, invertebrates, and fishes present in 40 marine lakes in Palau and Papua, and study how diversity has changed over time by retrieving the remains of organisms preserved in sediments on the lake bottoms. The project will test whether the number of species, the diversity of functional roles played by organisms, and the genetic diversity within species increase and decrease in parallel; whether certain species can greatly curtail diversity by changing the environment; whether the size of a lake determines its biodiversity; and whether the processes that control diversity in marine organisms are similar to those that operate on land.</p>
<p>Because biodiversity underlies the ecosystem services on which society depends, society has a great interest in understanding the processes that generate and retain biodiversity in nature. This project will also help conserve areas of economic importance. Marine lakes in the study region are important for tourism, and researchers will work closely with governmental and non-governmental conservation and education groups and with diving and tourism businesses to raise awareness of the value and threats to marine lakes in Indonesia and Palau.</p>
PaPaPro
largerWorkCitation
project
eng; USA
oceans
NW Pacific
134.3594
134.3594
7.153166667
7.153166667
2017-05-04
Western Pacific; Palau; Indonesia (West Papua)
0
BCO-DMO catalogue of parameters from Record of d2H of dinosterol variability in down core lake sediments from Clear Lake, Palau
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/699729.rdf
Name: location
Units: unitless
Description: <p>Name of the sampling location</p>
http://lod.bco-dmo.org/id/dataset-parameter/699730.rdf
Name: lat
Units: decimal degrees
Description: <p>Latitude of the location</p>
http://lod.bco-dmo.org/id/dataset-parameter/699731.rdf
Name: lon
Units: decimal degrees
Description: <p>Longitude of the location</p>
http://lod.bco-dmo.org/id/dataset-parameter/699732.rdf
Name: core_depth
Units: centimeters (cm)
Description: <p>Depth below sediment-water interface</p>
http://lod.bco-dmo.org/id/dataset-parameter/699733.rdf
Name: age
Units: unitless
Description: <p>Calendar age of sample in year A.D.</p>
http://lod.bco-dmo.org/id/dataset-parameter/699734.rdf
Name: dDdino
Units: per mil
Description: <p>d2Hdino: hydrogen isotopic composition of dinosterol</p>
http://lod.bco-dmo.org/id/dataset-parameter/699735.rdf
Name: dDdino_stdev
Units: per mil
Description: <p>1 sigma standard deviation on d2Hdino</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
4642
https://darchive.mblwhoilibrary.org/bitstream/1912/24413/1/dataset-699469_clear-lake-800-yr-reconstruction__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.699469.1
download
onLine
dataset
<p><strong>Sampling and analytical procedures:</strong><br />
Clear Lake sediments were extracted using a Dionex Accelerated Solvent Extractor (ASE 200), and the resulting total lipid extract was separated into neutral and polar fractions using column chromatography with aminopropyl gel as the stationary phase. The neutral fraction was then separated into hydrocarbon, wax ester, sterol and polar fractions via a silica gel column chromatography. Dinosterol was isolated then from the sterol fraction via reverse phase (RP)- high-performance liquid chromatography (HPLC)
.</p>
<p><strong>Instruments:</strong><br />
Dinosterol was isolated from the sterol fraction via reverse phase (RP)- high performance liquid chromatography (HPLC). An Agilent 1100 HPLC with an integrated autoinjector, quaternary pump, and fraction collector was coupled to an Agilent 1100 LC/MSD SL mass spectrometer with a multimode source that was operated in positive atmospheric pressure chemical ionization (APCI+) mode. The HPLC method used is outlined in Nelson and Sachs (2013).&nbsp;Subsequently to HPLC separation the fraction containing dinosterol was analyzed via GC-MSD to verify sufficient baseline separation. Adjacent HPLC fractions were also analyzed to ensure that no dinosterol eluted into those fractions.</p>
<p>After the dinosterol was sufficiently isolated from co-eluting compounds, the sample was injected onto a GC-irms for determination of the d<sup>2</sup>H of dinosterol.&nbsp; Hydrogen isotope determinations were made using a Finnigan Delta V Plus Isotope Ratio Mass Spectrometer (irMS) coupled to a Thermo Trace GC Ultra with a Varian VF-17ms FactorFour capillary column (60 m x 0.32 mm x 0.25 m) and a pyrolysis reactor. Samples were injected into a split/splitless inlet in splitless mode at 310 C. The oven temperature was ramped from 100 C to 220 C at a rate of 20 C/min, then at 2 C /min up to 325 C where it was held for 17 min. The carrier gas, He, was held constant at 2.6 mL/min. The pyroloysis reactor was maintained at 1400 C. Isotope values, expressed as D values, were calculated in Isodat software relative to VSMOW using a co-injection standard containing nC<sub>28</sub> nC<sub>32</sub>, nC<sub>40</sub>, and nC<sub>44</sub> of known ∂<sup>2</sup>H values (obtained from Arndt Schimmelmann, Indiana University, Bloomington, IN, USA). The measured isotope values of dinosterol were corrected for the addition of hydrogen atoms (of known D value) that occurred during acetylation. Each sample was analyzed in at least duplicate, and error bars represent standard deviations of replicate measurements.</p>
Specified by the Principal Investigator(s)
<p>BCO-DMO Processing:<br />
- modified parameter names to conform to BCO-DMO naming conventions;<br />
- replaced "NaN" with "nd".</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
high-performance liquid chromatography
high-performance liquid chromatography
PI Supplied Instrument Name: high-performance liquid chromatography PI Supplied Instrument Description:Dinosterol was isolated from the sterol fraction via reverse phase (RP)- high performance liquid chromatography (HPLC). An Agilent 1100 HPLC with an integrated autoinjector, quaternary pump, and fraction collector was coupled to an Agilent 1100 LC/MSD SL mass spectrometer with a multimode source that was operated in positive atmospheric pressure chemical ionization (APCI+) mode. Instrument Name: High-Performance Liquid Chromatograph Instrument Short Name:HPLC Instrument Description: A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB11/
Finnigan Delta V Plus Isotope Ratio Mass Spectrometer (irMS)
Finnigan Delta V Plus Isotope Ratio Mass Spectrometer (irMS)
PI Supplied Instrument Name: Finnigan Delta V Plus Isotope Ratio Mass Spectrometer (irMS) PI Supplied Instrument Description:Hydrogen isotope determinations were made using a Finnigan Delta V Plus Isotope Ratio Mass Spectrometer (irMS) coupled to a Thermo Trace GC Ultra with a Varian VF-17ms FactorFour capillary column (60 m x 0.32 mm x 0.25 m) and a pyrolysis reactor. Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Agilent 1100 LC/MSD SL mass spectrometer
Agilent 1100 LC/MSD SL mass spectrometer
PI Supplied Instrument Name: Agilent 1100 LC/MSD SL mass spectrometer PI Supplied Instrument Description:Dinosterol was isolated from the sterol fraction via reverse phase (RP)- high performance liquid chromatography (HPLC). An Agilent 1100 HPLC with an integrated autoinjector, quaternary pump, and fraction collector was coupled to an Agilent 1100 LC/MSD SL mass spectrometer with a multimode source that was operated in positive atmospheric pressure chemical ionization (APCI+) mode. Instrument Name: Mass Spectrometer Instrument Short Name:Mass Spec Instrument Description: General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Cruise: Palau_lakes
Palau_lakes
Small boats - CRRF
vessel
Palau_lakes
Michael N. Dawson
University of California-Merced
Small boats - CRRF
vessel