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            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset/700974.rdf" xlink:actuate="onRequest">Environmental and physical data associated with ocean acidification microbe adaptation from 2012-2014</gmx:Anchor>
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            <gco:CharacterString>Cite this dataset as: Johnson, Z., Hunt, D. (2017) Environmental and physical data associated with ocean acidification microbe adaptation from 2012-2014. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2017-05-25 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.700974.1 [access date]</gco:CharacterString>
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        <gco:CharacterString>Environmental and physical data associated with ocean acidification microbe adaptation. Dataset Description: &amp;lt;p&amp;gt;Environmental and physical data associated with ocean acidification microbe adaptation.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;For related research, go to:&amp;amp;nbsp;&amp;lt;a href=&amp;quot;http://oceanography.ml.duke.edu/johnson/research/pico/&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;http://oceanography.ml.duke.edu/johnson/research/pico/&amp;lt;/a&amp;gt;&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;DIC:&amp;amp;nbsp;&amp;lt;/strong&amp;gt;Water was sampled using a 5 L niskin bottle centered at 1 m with a bottle length of 0.7 m.&amp;amp;nbsp; DIC was measured on mercuric chloride poisoned samples by acidification and subsequent quantification of released CO2&amp;amp;nbsp;using a CO2&amp;amp;nbsp;detector (Li-Cor 7000). DIC samples were collected following recommended procedures {Dickson et al., 2007} and measurements were calibrated against Certified Reference Materials provided by Dr. A. G. Dickson at Scripps Institution of Oceanography (SIO), University of California, San Diego (UCSD). &amp;amp;nbsp;&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;pH:&amp;amp;nbsp;&amp;lt;/strong&amp;gt;Water was sampled using a 5 L niskin bottle centered at 1 m with a bottle length of 0.7 m.&amp;amp;nbsp; pH was measured spectrophotometrically {Clayton and Byrne, 1993} in triplicate at standard temperature (25°C) immediately following collection. pH samples were collected following recommended procedures {Dickson et al., 2007}.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Secchi Depth:&amp;lt;/strong&amp;gt; Secchi depth was measured in duplicate using a 20 cm disk with four alternating white and black quadrants (Cole Parmer #EW-05492-00) by lowering the disk until no longer visible and recording the depth.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Salinity:&amp;lt;/strong&amp;gt; Water was sampled using a 5 L niskin bottle centered at 1 m with a bottle length of 0.7 m.&amp;amp;nbsp; Salinity was measured using a calibrated handheld digital refractometer (Atago PAL-06S), using a refractometer (Vista A366ATC), or using a Guideline Portasal 8410A all according to manufacturer’s instructions and calibrated against known reference materials.&amp;amp;nbsp; In situ salinity at the same depth was measured using a YSI Pro30.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Turbidity:&amp;amp;nbsp;&amp;lt;/strong&amp;gt;Turbidity was measured in duplicate on discrete samples using a calibrated handheld turbidimeter (Orion AQ4500).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Dissolved&amp;amp;nbsp;Oxygen:&amp;lt;/strong&amp;gt; Oxygen was measured optically&amp;amp;nbsp;in situ&amp;amp;nbsp;and atmospheric pressure measured near the sea surface using a calibrated probe (YSI ProODO) using manufactures recommendations.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Chlorophyll:&amp;lt;/strong&amp;gt; Water was sampled using a 5 L niskin bottle centered at 1 m with a bottle length of 0.7 m. Methods described in Johnson et al. 2010: Chlorophyll concentrations were measured by filtering 25&amp;amp;nbsp; mL of seawater sample onto a 0.22 µm pore size polycarbonate filter using gentle vacuum (&amp;amp;lt;100 mm Hg) and extracting in 100% MeOH at -20°C in the dark for &amp;amp;gt;24 h following (Holm-Hansen and Riemann, 1978).&amp;amp;nbsp; Fluorescence was measured using a Turner Designs 10-AU fluorometer following (Welschmeyer, 1994) that was calibrated against a standard chlorophyll solution (Ritchie, 2008).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Bacteria:&amp;amp;nbsp;&amp;lt;/strong&amp;gt;Bacterioplankton (i.e. ‘bacteria’) were enumerated using a FACSCalibur flow cytometer (Becton Dickinson) and populations characterized as previously described (Johnson et al., 2010).&amp;amp;nbsp; Briefly, cells were excited with a 488 nm laser (15 mW Ar) and inelastic forward (&amp;amp;lt;15°) scatter, inelastic side (90°) scatter (SSC), green (530 ± 30 nm) fluorescence, orange fluorescence (585 ± 42 nm), and red fluorescence (&amp;amp;gt; 670 nm) emissions were measured.&amp;amp;nbsp; Bacterioplankton were quantified by staining the samples with the nucleic acid stain SYBR Green –I (Molecular Probes Inc.) (Marie et al., 1997).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Nutrients:&amp;lt;/strong&amp;gt; Water was filtered through a 0.22 µm Sterivex cartridge filter,&amp;amp;nbsp; Millipore #SVGPL10RC using a peristaltic pump input line at 1 m for later nutrient analysis (NO3, NO2, PO4, SiOH4)&amp;amp;nbsp; and water was placed into duplicate HCl-cleaned HDPE bottles (VWR#414004-110) and stored at -80°C until later analysis using an Astoria-Pacific A2 autoanalyzer following the manufacturer’s recommended protocols running each replicate sample in duplicate. &amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Certified reference materials were used to verify protocols (Inorganic Ventures: QCP-NT, QCP-NUT-1, CGSI1-1).&amp;amp;nbsp; The detection limit was NO2&amp;amp;nbsp;= 0.05 µM, NO3&amp;amp;nbsp;= 0.1 µM, PO4&amp;amp;nbsp;= 0.05 µM, SiOH4&amp;amp;nbsp;= 0.2 µM).&amp;amp;nbsp; Values measured below these limits are reported as zero.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Temperature:&amp;amp;nbsp;&amp;lt;/strong&amp;gt;Water was sampled using a 5 L niskin bottle centered at 1 m with a bottle length of 0.7 m. Temperature was measured in duplicate using NIST traceable thermocouples (VWR#23609-232).&amp;amp;nbsp; In situ water temperature at the same depth was measured using a YSI Pro30. &amp;amp;nbsp;&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/535565.rdf" xlink:title="OCE-1416665" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1416665 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1416665</gmx:Anchor>
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In recognition of the need for basic research concerning the nature, extent and impact of ocean acidification on oceanic environments in the past, present and future, the goal of the SEES: OA program is to understand (a) the chemistry and physical chemistry of ocean acidification; (b) how ocean acidification interacts with processes at the organismal level; and (c) how the earth system history informs our understanding of the effects of ocean acidification on the present day and future ocean.
Solicitations issued under this program:
NSF 10-530, FY 2010-FY2011
NSF 12-500, FY 2012
NSF 12-600, FY 2013
NSF 13-586, FY 2014
NSF 13-586 was the final solicitation that will be released for this program.
PI Meetings:
1st U.S. Ocean Acidification PI Meeting(March 22-24, 2011, Woods Hole, MA)
2nd U.S. Ocean Acidification PI Meeting(Sept. 18-20, 2013, Washington, DC)
3rd U.S. Ocean Acidification PI Meeting (June 9-11, 2015, Woods Hole, MA – Tentative)
NSF media releases for the Ocean Acidification Program:
Press Release 10-186 NSF Awards Grants to Study Effects of Ocean Acidification
Discovery Blue Mussels &quot;Hang On&quot; Along Rocky Shores: For How Long?
Discovery nsf.gov - National Science Foundation (NSF) Discoveries - Trouble in Paradise: Ocean Acidification This Way Comes - US National Science Foundation (NSF)
Press Release 12-179 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: Finding New Answers Through National Science Foundation Research Grants - US National Science Foundation (NSF)
Press Release 13-102 World Oceans Month Brings Mixed News for Oysters
Press Release 13-108 nsf.gov - National Science Foundation (NSF) News - Natural Underwater Springs Show How Coral Reefs Respond to Ocean Acidification - US National Science Foundation (NSF)
Press Release 13-148 Ocean acidification: Making new discoveries through National Science Foundation research grants
Press Release 13-148 - Video nsf.gov - News - Video - NSF Ocean Sciences Division Director David Conover answers questions about ocean acidification. - US National Science Foundation (NSF)
Press Release 14-010 nsf.gov - National Science Foundation (NSF) News - Palau's coral reefs surprisingly resistant to ocean acidification - US National Science Foundation (NSF)
Press Release 14-116 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: NSF awards $11.4 million in new grants to study effects on marine ecosystems - US National Science Foundation (NSF)</gco:CharacterString>
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                            <gco:CharacterString>&lt;p&gt;&lt;em&gt;From the &lt;/em&gt;&lt;a href=&quot;http://oceanography.ml.duke.edu/johnson/research/pico/&quot; target=&quot;_blank&quot;&gt;&lt;em&gt;project website&lt;/em&gt;&lt;/a&gt;&lt;em&gt;:&lt;/em&gt;&lt;br /&gt;
Carbon dioxide is rising at ~3% per year in the atmosphere and oceans leading to increases in dissolved inorganic carbon and a reduction in pH. This trend is expected to continue for the foreseeable future and ocean pH is predicted to decrease substantially making the ocean more acidic, potentially affecting the marine ecosystem. However, coastal estuaries are highly dynamic systems that often experience dramatic changes in environmental variables over short periods of times. In this study, the investigators are measuring key variables of the marine carbon system along with other potential forcing variables and characteristics of the ecosystem that may be affected by these pH changes. The goal of this project is to determine the time-scales and magnitude of natural variability that will be superimposed on any long term trends in ocean chemistry.&lt;/p&gt;
&lt;p&gt;&lt;strong&gt;Other PICO-related projects in BCO-DMO:&lt;/strong&gt;&lt;br /&gt;
&lt;a href=&quot;https://www.bco-dmo.org/project/535566&quot; target=&quot;_blank&quot;&gt;Ocean Acidification: microbes as sentinels of adaptive responses to multiple stressors: contrasting estuarine and open ocean environments&lt;/a&gt;&lt;/p&gt;
&lt;p&gt;&lt;a href=&quot;https://www.bco-dmo.org/project/941303&quot; target=&quot;_blank&quot;&gt;Collaborative Research: BoCP-Design: A multidomain microbial consortium to interrogate organic matter decomposition in a changing ocean&lt;/a&gt;&lt;/p&gt;
&lt;p&gt;&lt;a href=&quot;https://www.bco-dmo.org/project/941318&quot; target=&quot;_blank&quot;&gt;NSF2026: EAGER: Identifying microbes’ population-level environmental responses using Bayesian modeling&lt;/a&gt;&lt;/p&gt;</gco:CharacterString>
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                            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/project/535566.rdf" xlink:title="Project Name" xlink:actuate="onRequest">Collaborative Research: Ocean Acidification: microbes as sentinels of adaptive responses to multiple stressors: contrasting estuarine and open ocean environments</gmx:Anchor>
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                            <gco:CharacterString>&lt;p&gt;&lt;em&gt;Extracted from the NSF award abstract:&lt;/em&gt;&lt;/p&gt;
&lt;p&gt;This collaborative project by Duke University and Georgia Institute of Technology researchers will combine oceanographic and advanced molecular techniques to characterize the adaptive responses of microbial communities to multiple stressors associated with OA. In particular, microbial communities from estuarine and coastal ecosystems as well as open ocean waters will be incubated under conditions of increased acidity or temperature or both, and their activities will be measured and quantified. &lt;/p&gt;
&lt;p&gt;
Preliminary data from time-series observations of a coastal temperate estuary shows that pH, temperature and other stressors vary over multiple space and time scales, and this variability is relatively higher than that observed in open ocean waters. Based on this evidence, the guiding hypothesis of this work is that microbes in coastal ecosystems are better adapted to ocean acidification as well as multiple stressors compared to similar microbes from the open ocean. To quantify the adaptive genetic, physiological and biogeochemical responses of microbes to OA, the team's specific goals are to: (1) characterize complex natural microbial community responses to multiple stressors using factorial mesocosm manipulations, (2) assemble a detailed view of genomic and physiological (including transcriptional) adaptations to OA at the single species level using cultured model marine microbes (e.g. Prochlorococcus, Synechococcus, Vibrio) identified as responsive to stressors in whole community mesocosm experiments, and (3) assess the power of model microbial strains and mesocosm experiments to predict microbial community responses to natural OA variability in a temporally dynamic, temperate estuary and along a trophic/pH gradient from the Neuse-Pamlico Sound to the Sargasso Sea. By comparing an estuarine ecosystem to its open ocean counterpart, this study will assess the sensitivity of microbial structure and function in response to ocean acidification.&lt;/p&gt;
&lt;p&gt;This project is associated with &lt;a href=&quot;https://www.bco-dmo.org/project/2281&quot; target=&quot;_blank&quot;&gt;Pivers Island Coastal Observatory&lt;/a&gt;.&lt;/p&gt;</gco:CharacterString>
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	Name: date
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http://lod.bco-dmo.org/id/dataset-parameter/701587.rdf
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	Units: cells per milliliter
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	Units: hectopascals
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	Units: milligrams of chlorophyll a per meter cubed
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http://lod.bco-dmo.org/id/dataset-parameter/701598.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/701599.rdf
	Name: NO2B
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http://lod.bco-dmo.org/id/dataset-parameter/701600.rdf
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	Units: uM
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	Units: uM
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http://lod.bco-dmo.org/id/dataset-parameter/701603.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/701604.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/701606.rdf
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	Units: milligrams of oxygen per liter
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http://lod.bco-dmo.org/id/dataset-parameter/701612.rdf
	Name: PO4A
	Units: uM
	Description: &lt;p&gt;Inorganic nutrient concentration; Sample A&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701613.rdf
	Name: PO4B
	Units: uM
	Description: &lt;p&gt;Inorganic nutrient concentration; Sample B&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701614.rdf
	Name: PO4C
	Units: uM
	Description: &lt;p&gt;Inorganic nutrient concentration; Sample C&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701615.rdf
	Name: QbacteriaA
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701616.rdf
	Name: QbacteriaB
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701617.rdf
	Name: QBarometricPressureA
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701618.rdf
	Name: QBarometricPressureB
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701619.rdf
	Name: QChlExtractA
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701620.rdf
	Name: QChlExtractB
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701621.rdf
	Name: QDepth
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701622.rdf
	Name: QDICA
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701623.rdf
	Name: QDICB
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701624.rdf
	Name: QDICC
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701625.rdf
	Name: QNH4A
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701626.rdf
	Name: QNH4B
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701627.rdf
	Name: QNH4C
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701628.rdf
	Name: QNO2A
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701629.rdf
	Name: QNO2B
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701630.rdf
	Name: QNO2C
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701631.rdf
	Name: QNO3A
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701632.rdf
	Name: QNO3B
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701633.rdf
	Name: QNO3C
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701634.rdf
	Name: QOxygenA
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701635.rdf
	Name: QOxygenB
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701636.rdf
	Name: QOxygenSaturationA
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701637.rdf
	Name: QOxygenSaturationB
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701638.rdf
	Name: QpHT25A
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701639.rdf
	Name: QpHT25B
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701640.rdf
	Name: QpHT25C
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701641.rdf
	Name: QPO4A
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701642.rdf
	Name: QPO4B
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701643.rdf
	Name: QPO4C
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701644.rdf
	Name: QSalinityAtagoA
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701645.rdf
	Name: QSalinityAtagoB
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701646.rdf
	Name: QSalinityPortasalA
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701647.rdf
	Name: QSalinityPortasalB
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701648.rdf
	Name: QSalinityPro30A
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701649.rdf
	Name: QSalinityPro30B
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701650.rdf
	Name: QSalinitySpyGlassA
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701651.rdf
	Name: QSalinitySpyGlassB
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701652.rdf
	Name: QSecchiDepthA
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701653.rdf
	Name: QSecchiDepthB
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701654.rdf
	Name: QSiOH4A
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701655.rdf
	Name: QSiOH4B
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701656.rdf
	Name: QSiOH4C
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701657.rdf
	Name: QTempBottleA
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701658.rdf
	Name: QTempBottleB
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701659.rdf
	Name: QTempPro30ProbeA
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701660.rdf
	Name: QTempPro30ProbeB
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701661.rdf
	Name: QTurbidityA
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701662.rdf
	Name: QTurbidityB
	Units: unitless
	Description: &lt;p&gt;Quality score: 1=excellent (no known issues); 2=suspect; 3=poor (known reason to suspect data)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701663.rdf
	Name: SalinityAtagoA
	Units: PSU
	Description: &lt;p&gt;Salinity measurement by instrument; Sample A&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701664.rdf
	Name: SalinityAtagoB
	Units: PSU
	Description: &lt;p&gt;Salinity measurement by instrument; Sample B&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701665.rdf
	Name: SalinityPortasalA
	Units: PSU
	Description: &lt;p&gt;Salinity measurement by instrument; Sample A&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701666.rdf
	Name: SalinityPortasalB
	Units: PSU
	Description: &lt;p&gt;Salinity measurement by instrument; Sample B&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701667.rdf
	Name: SalinityPro30A
	Units: PSU
	Description: &lt;p&gt;Salinity measurement by instrument; Sample A&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701668.rdf
	Name: SalinityPro30B
	Units: PSU
	Description: &lt;p&gt;Salinity measurement by instrument; Sample B&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701669.rdf
	Name: SalinitySpyGlassA
	Units: PSU
	Description: &lt;p&gt;Salinity measurement by instrument; Sample A&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701670.rdf
	Name: SalinitySpyGlassB
	Units: PSU
	Description: &lt;p&gt;Salinity measurement by instrument; Sample B&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701671.rdf
	Name: SecchiDepthA
	Units: meters
	Description: &lt;p&gt;Secchi depth; Sample A&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701672.rdf
	Name: SecchiDepthB
	Units: meters
	Description: &lt;p&gt;Secchi depth; Sample B&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701673.rdf
	Name: SiOH4A
	Units: uM
	Description: &lt;p&gt;Inorganic nutrient concentration; Sample A&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701674.rdf
	Name: SiOH4B
	Units: uM
	Description: &lt;p&gt;Inorganic nutrient concentration; Sample B&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701675.rdf
	Name: SiOH4C
	Units: uM
	Description: &lt;p&gt;Inorganic nutrient concentration; Sample C&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701676.rdf
	Name: TempBottleA
	Units: Celsius
	Description: &lt;p&gt;Temperature; Sample A&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701677.rdf
	Name: TempBottleB
	Units: Celsius
	Description: &lt;p&gt;Temperature; Sample B&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701678.rdf
	Name: TempPro30ProbeA
	Units: Celsius
	Description: &lt;p&gt;Temperature from Pro 30 Probe; Sample A&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701679.rdf
	Name: TempPro30ProbeB
	Units: Celsius
	Description: &lt;p&gt;Temperature from Pro 30 Probe; Sample B&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701680.rdf
	Name: TurbidityA
	Units: Nephelometric turbidity units (NTU)
	Description: &lt;p&gt;Turbidity; Sample A&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701681.rdf
	Name: TurbidityB
	Units: Nephelometric turbidity units (NTU)
	Description: &lt;p&gt;Turbidity; Sample B&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/701682.rdf
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                <gco:CharacterString>&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;DIC:&amp;amp;nbsp;&amp;lt;/strong&amp;gt;Water was sampled using a 5 L niskin bottle centered at 1 m with a bottle length of 0.7 m.&amp;amp;nbsp; DIC was measured on mercuric chloride poisoned samples by acidification and subsequent quantification of released CO2&amp;amp;nbsp;using a CO2&amp;amp;nbsp;detector (Li-Cor 7000). DIC samples were collected following recommended procedures {Dickson et al., 2007} and measurements were calibrated against Certified Reference Materials provided by Dr. A. G. Dickson at Scripps Institution of Oceanography (SIO), University of California, San Diego (UCSD). &amp;amp;nbsp;&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;pH:&amp;amp;nbsp;&amp;lt;/strong&amp;gt;Water was sampled using a 5 L niskin bottle centered at 1 m with a bottle length of 0.7 m.&amp;amp;nbsp; pH was measured spectrophotometrically {Clayton and Byrne, 1993} in triplicate at standard temperature (25°C) immediately following collection. pH samples were collected following recommended procedures {Dickson et al., 2007}.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Secchi Depth:&amp;lt;/strong&amp;gt; Secchi depth was measured in duplicate using a 20 cm disk with four alternating white and black quadrants (Cole Parmer #EW-05492-00) by lowering the disk until no longer visible and recording the depth.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Salinity:&amp;lt;/strong&amp;gt; Water was sampled using a 5 L niskin bottle centered at 1 m with a bottle length of 0.7 m.&amp;amp;nbsp; Salinity was measured using a calibrated handheld digital refractometer (Atago PAL-06S), using a refractometer (Vista A366ATC), or using a Guideline Portasal 8410A all according to manufacturer’s instructions and calibrated against known reference materials.&amp;amp;nbsp; In situ salinity at the same depth was measured using a YSI Pro30.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Turbidity:&amp;amp;nbsp;&amp;lt;/strong&amp;gt;Turbidity was measured in duplicate on discrete samples using a calibrated handheld turbidimeter (Orion AQ4500).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Dissolved&amp;amp;nbsp;Oxygen:&amp;lt;/strong&amp;gt; Oxygen was measured optically&amp;amp;nbsp;in situ&amp;amp;nbsp;and atmospheric pressure measured near the sea surface using a calibrated probe (YSI ProODO) using manufactures recommendations.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Chlorophyll:&amp;lt;/strong&amp;gt; Water was sampled using a 5 L niskin bottle centered at 1 m with a bottle length of 0.7 m. Methods described in Johnson et al. 2010: Chlorophyll concentrations were measured by filtering 25&amp;amp;nbsp; mL of seawater sample onto a 0.22 µm pore size polycarbonate filter using gentle vacuum (&amp;amp;lt;100 mm Hg) and extracting in 100% MeOH at -20°C in the dark for &amp;amp;gt;24 h following (Holm-Hansen and Riemann, 1978).&amp;amp;nbsp; Fluorescence was measured using a Turner Designs 10-AU fluorometer following (Welschmeyer, 1994) that was calibrated against a standard chlorophyll solution (Ritchie, 2008).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Bacteria:&amp;amp;nbsp;&amp;lt;/strong&amp;gt;Bacterioplankton (i.e. ‘bacteria’) were enumerated using a FACSCalibur flow cytometer (Becton Dickinson) and populations characterized as previously described (Johnson et al., 2010).&amp;amp;nbsp; Briefly, cells were excited with a 488 nm laser (15 mW Ar) and inelastic forward (&amp;amp;lt;15°) scatter, inelastic side (90°) scatter (SSC), green (530 ± 30 nm) fluorescence, orange fluorescence (585 ± 42 nm), and red fluorescence (&amp;amp;gt; 670 nm) emissions were measured.&amp;amp;nbsp; Bacterioplankton were quantified by staining the samples with the nucleic acid stain SYBR Green –I (Molecular Probes Inc.) (Marie et al., 1997).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Nutrients:&amp;lt;/strong&amp;gt; Water was filtered through a 0.22 µm Sterivex cartridge filter,&amp;amp;nbsp; Millipore #SVGPL10RC using a peristaltic pump input line at 1 m for later nutrient analysis (NO3, NO2, PO4, SiOH4)&amp;amp;nbsp; and water was placed into duplicate HCl-cleaned HDPE bottles (VWR#414004-110) and stored at -80°C until later analysis using an Astoria-Pacific A2 autoanalyzer following the manufacturer’s recommended protocols running each replicate sample in duplicate. &amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Certified reference materials were used to verify protocols (Inorganic Ventures: QCP-NT, QCP-NUT-1, CGSI1-1).&amp;amp;nbsp; The detection limit was NO2&amp;amp;nbsp;= 0.05 µM, NO3&amp;amp;nbsp;= 0.1 µM, PO4&amp;amp;nbsp;= 0.05 µM, SiOH4&amp;amp;nbsp;= 0.2 µM).&amp;amp;nbsp; Values measured below these limits are reported as zero.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Temperature:&amp;amp;nbsp;&amp;lt;/strong&amp;gt;Water was sampled using a 5 L niskin bottle centered at 1 m with a bottle length of 0.7 m. Temperature was measured in duplicate using NIST traceable thermocouples (VWR#23609-232).&amp;amp;nbsp; In situ water temperature at the same depth was measured using a YSI Pro30. &amp;amp;nbsp;&amp;lt;/p&amp;gt;</gco:CharacterString>
              </gmd:description>
              <gmd:source>
                <gmd:LI_Source>
                  <gmd:sourceCitation>
                    <gmd:CI_Citation>
                      <gmd:title>
                        <gco:CharacterString>Specified by the Principal Investigator(s)</gco:CharacterString>
                      </gmd:title>
                      <gmd:date gco:nilReason="unknown"/>
                    </gmd:CI_Citation>
                  </gmd:sourceCitation>
                </gmd:LI_Source>
              </gmd:source>
            </gmd:LI_ProcessStep>
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          <gmd:processStep xlink:title="Data Processing Description">
            <gmd:LI_ProcessStep>
              <gmd:description>
                <gco:CharacterString>&amp;lt;p&amp;gt;Quality Scores (Q) as follows: 1=excellent (no known issues), 2=suspect, 3=poor (known reason to suspect data)&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Nutrients:&amp;amp;nbsp;&amp;lt;/strong&amp;gt;Samples that had a mean concentration (mean of replicated samples) below the nominal detection limit are reported as zero. &amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Bacteria:&amp;amp;nbsp;&amp;lt;/strong&amp;gt;Cells counts were normalized to volume sampled to determined cells per mL.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Chlorophyll:&amp;lt;/strong&amp;gt;&amp;amp;nbsp;&amp;amp;gt;0.22 um referred to as “total” or simply “chlorophyll”&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;BCO-DMO Data Processing Notes:&amp;lt;/strong&amp;gt;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;- replaced NaN with nd&amp;lt;br /&amp;gt;
- added ISO DateTime column&amp;lt;br /&amp;gt;
- separated date and time into two columns&amp;lt;/p&amp;gt;</gco:CharacterString>
              </gmd:description>
              <gmd:source>
                <gmd:LI_Source>
                  <gmd:sourceCitation>
                    <gmd:CI_Citation>
                      <gmd:title>
                        <gco:CharacterString>Specified by the Principal Investigator(s)</gco:CharacterString>
                      </gmd:title>
                      <gmd:date gco:nilReason="unknown"/>
                    </gmd:CI_Citation>
                  </gmd:sourceCitation>
                </gmd:LI_Source>
              </gmd:source>
            </gmd:LI_ProcessStep>
          </gmd:processStep>
        </gmd:LI_Lineage>
      </gmd:lineage>
   </gmd:DQ_DataQuality>
  </gmd:dataQualityInfo>
  <gmd:metadataMaintenance>
    <gmd:MD_MaintenanceInformation>
      <gmd:maintenanceAndUpdateFrequency>
        <gmd:MD_MaintenanceFrequencyCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#MD_MaintenanceFrequencyCode" codeListValue="asNeeded" codeSpace="009">asNeeded</gmd:MD_MaintenanceFrequencyCode>
      </gmd:maintenanceAndUpdateFrequency>
      <gmd:maintenanceNote>
        <gco:CharacterString>7.x-1.1</gco:CharacterString>
      </gmd:maintenanceNote>
      <gmd:contact>
        <gmd:CI_ResponsibleParty>
  <gmd:organisationName>
    <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/affiliation/191.rdf" xlink:actuate="onRequest">Biological and Chemical Oceanography Data Management Office (BCO-DMO)</gmx:Anchor>
  </gmd:organisationName>
  <gmd:contactInfo>
    <gmd:CI_Contact>
		  <gmd:phone>
		    <gmd:CI_Telephone>
				  <gmd:voice>
				    <gco:CharacterString>Unavailable</gco:CharacterString>
				  </gmd:voice>
				  <gmd:facsimile>
				    <gco:CharacterString>508-289-2009</gco:CharacterString>
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		    <gmd:CI_Address>
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				    <gco:CharacterString>WHOI MS#36</gco:CharacterString>
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				  <gmd:city>
				    <gco:CharacterString>Woods Hole</gco:CharacterString>
				  </gmd:city>
				  <gmd:administrativeArea>
				    <gco:CharacterString>MA</gco:CharacterString>
				  </gmd:administrativeArea>
				  <gmd:postalCode>
				    <gco:CharacterString>02543</gco:CharacterString>
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				  <gmd:country>
				    <gco:CharacterString>USA</gco:CharacterString>
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				  <gmd:electronicMailAddress>
				    <gco:CharacterString>info@bco-dmo.org</gco:CharacterString>
				  </gmd:electronicMailAddress>
		    </gmd:CI_Address>
		  </gmd:address>
      <gmd:onlineResource>
          <gmd:CI_OnlineResource>
            <gmd:linkage>
              <gmd:URL>http://www.bco-dmo.org</gmd:URL>
            </gmd:linkage>
          </gmd:CI_OnlineResource>
        </gmd:onlineResource>
		  <gmd:hoursOfService>
        <gco:CharacterString>Monday - Friday 8:00am - 5:00pm</gco:CharacterString>
      </gmd:hoursOfService>
		  <gmd:contactInstructions>
		    <gco:CharacterString>For questions regarding this resource, please contact BCO-DMO via the email address provided.</gco:CharacterString>
		  </gmd:contactInstructions>
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  </gmd:contactInfo>
  <gmd:role>
    <gmd:CI_RoleCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#CI_RoleCode" codeListValue="pointOfContact"  codeSpace="007">pointOfContact</gmd:CI_RoleCode>
  </gmd:role>
</gmd:CI_ResponsibleParty>
      </gmd:contact>
    </gmd:MD_MaintenanceInformation>
  </gmd:metadataMaintenance>
  <gmi:acquisitionInformation>
    <gmi:MI_AcquisitionInformation>
    <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/542498.rdf" xlink:title="Bottle" xlink:actuate="onRequest">HDPE bottle</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>HDPE bottle</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: HDPE bottle PI Supplied Instrument Description:Used in nutrient analysis Instrument Name: Bottle Instrument Short Name:Bottle   Instrument Description: A container, typically made of glass or plastic and with a narrow neck, used for storing drinks or other liquids.</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/491476.rdf" xlink:title="CO2 Analyzer" xlink:actuate="onRequest">Li-Cor 7000</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>Li-Cor 7000</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Li-Cor 7000 PI Supplied Instrument Description:Used to sample DIC Instrument Name: CO2 Analyzer Instrument Short Name:CO2 Analyzer   Instrument Description: Measures atmospheric carbon dioxide (CO2) concentration. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/382/</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/685040.rdf" xlink:title="digital thermometer" xlink:actuate="onRequest">NIST traceable thermocouples</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>NIST traceable thermocouples</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: NIST traceable thermocouples PI Supplied Instrument Description:Used to measure temperature Instrument Name: digital thermometer Instrument Short Name:   Instrument Description: An instrument that measures temperature digitally.</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/484.rdf" xlink:title="Fluorometer" xlink:actuate="onRequest">10-AU Turner Designs Fluorometer</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>10-AU Turner Designs Fluorometer</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: 10-AU Turner Designs Fluorometer PI Supplied Instrument Description:Used to measure fluorescence Instrument Name: Fluorometer Instrument Short Name:Fluorometer   Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/413.rdf" xlink:title="Niskin bottle" xlink:actuate="onRequest">Niskin</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>Niskin</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Niskin PI Supplied Instrument Description:Used to collect water samples Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle   Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/558.rdf" xlink:title="Nutrient Autoanalyzer" xlink:actuate="onRequest">Astoria-Pacific A2 autoanalyzer</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>Astoria-Pacific A2 autoanalyzer</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Astoria-Pacific A2 autoanalyzer PI Supplied Instrument Description:Used in nutrient analysis Instrument Name: Nutrient Autoanalyzer Instrument Short Name:Nutrient Autoanalyzer   Instrument Description: Nutrient Autoanalyzer is a generic term used when specific type, make and model were not specified.  In general, a Nutrient Autoanalyzer is an automated flow-thru system for doing nutrient analysis (nitrate, ammonium, orthophosphate, and silicate) on seawater samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/705.rdf" xlink:title="Oxygen Sensor" xlink:actuate="onRequest">YSI ProODO</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>YSI ProODO</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: YSI ProODO PI Supplied Instrument Description:Used to measure dissolved oxygen Instrument Name: Oxygen Sensor Instrument Short Name:Dissolved Oxygen Sensor   Instrument Description: An electronic device that measures the proportion of oxygen (O2) in the gas or liquid being analyzed</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/726.rdf" xlink:title="Pump" xlink:actuate="onRequest">Peristaltic pump</gmx:Anchor>
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          <gmi:type>
            <gco:CharacterString>Peristaltic pump</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Peristaltic pump PI Supplied Instrument Description:Used in nutrient analysis Instrument Name: Pump Instrument Short Name:   Instrument Description: A pump is a device that moves fluids (liquids or gases), or sometimes slurries, by mechanical action. Pumps can be classified into three major groups according to the method they use to move the fluid: direct lift, displacement, and gravity pumps</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/679.rdf" xlink:title="Refractometer" xlink:actuate="onRequest">Atago PAL-06S Digital Refractometer</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>Atago PAL-06S Digital Refractometer</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Atago PAL-06S Digital Refractometer PI Supplied Instrument Description:Used to sample salinity Instrument Name: Refractometer Instrument Short Name:Refractometer   Instrument Description: A refractometer is a laboratory or field device for the measurement of an index of refraction (refractometry). The index of refraction is calculated from Snell's law and can be calculated from the composition of the material using the Gladstone-Dale relation.

In optics the refractive index (or index of refraction) n of a substance (optical medium) is a dimensionless number that describes how light, or any other radiation, propagates through that medium.</gco:CharacterString>
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        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
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            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/667.rdf" xlink:title="Spectrometer" xlink:actuate="onRequest">Spectrometer</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>Spectrometer</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Spectrometer PI Supplied Instrument Description:Used to sample pH Instrument Name: Spectrometer Instrument Short Name:Spectrometer   Instrument Description: A spectrometer is an optical instrument used to measure properties of light over a specific portion of the electromagnetic spectrum. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0460/</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/721.rdf" xlink:title="Turbidity Meter" xlink:actuate="onRequest">Orion AQ4500</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>Orion AQ4500</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Orion AQ4500 PI Supplied Instrument Description:Used to analyze turbidity Instrument Name: Turbidity Meter Instrument Short Name:Turbidity Meter   Instrument Description: A turbidity meter measures the clarity of a water sample. A beam of light is shown through a water sample. The turbidity, or its converse clarity, is read on a numerical scale. Turbidity determined by this technique is referred to as the nephelometric method from the root meaning &quot;cloudiness&quot;. This word is used to form the name of the unit of turbidity, the NTU (Nephelometric Turbidity Unit). The meter reading cannot be used to compare the turbidity of different water samples unless the instrument is calibrated. Description from: http://www.gvsu.edu/wri/education/instructor-s-manual-turbidity-10.htm
(One example is the Orion AQ4500 Turbidimeter)</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:operation>
          <gmi:MI_Operation>
            <gmi:description>
              <gco:CharacterString>Deployment: PICO_1-301</gco:CharacterString>
            </gmi:description>
            <gmi:identifier>
              <gmd:MD_Identifier>
                <gmd:code>
                  <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/deployment/59063.rdf" xlink:title="Deployment" xlink:actuate="onRequest">PICO_1-301</gmx:Anchor>
                </gmd:code>
              </gmd:MD_Identifier>
            </gmi:identifier>
            <gmi:status>
              <gmd:MD_ProgressCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MD_ProgressCode" codeListValue="completed"/>
            </gmi:status>
            <gmi:type>
              <gmi:MI_OperationTypeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MI_OperationTypeCode" codeListValue="real"/>
            </gmi:type>
            <gmi:parentOperation gco:nilReason="inapplicable"/>
            <gmi:platform>
  <gmi:MI_Platform>
    <gmi:identifier>
      <gmd:MD_Identifier>
        <gmd:code>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/54275.rdf"
           xlink:actuate="onRequest">Duke University Marine Lab</gmx:Anchor>
        </gmd:code>
      </gmd:MD_Identifier>
    </gmi:identifier>
    <gmi:description>
      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/54275.rdf" xlink:title="Duke University Marine Lab" xlink:actuate="onRequest">dock</gmx:Anchor>
    </gmi:description>
    <gmi:instrument gco:nilReason="unknown"/>
  </gmi:MI_Platform>
</gmi:platform>
            <gmi:plan>
              <gmi:MI_Plan>
                <gmi:status>
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                <gmi:citation>
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                      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/deployment/59063.rdf" xlink:title="Deployment" xlink:actuate="onRequest">PICO_1-301</gmx:Anchor>
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            </gmi:MI_Operation>
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          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/54275.rdf"
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    <gmi:description>
      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/54275.rdf" xlink:title="Duke University Marine Lab" xlink:actuate="onRequest">dock</gmx:Anchor>
    </gmi:description>
    <gmi:instrument gco:nilReason="unknown"/>
  </gmi:MI_Platform>
</gmi:platform>
          </gmi:MI_AcquisitionInformation>
  </gmi:acquisitionInformation>
</gmi:MI_Metadata>
