<div><p>Multiple hydrocasts conducted at stations denoted in the bottle file meta data. A SeaBird CTD was used with redundant sensors for temperature and conductivity; the instrument package was owned and operated by the R/V Point Sur (Moss Landing Marine Laboratories, Moss Landing, California). All hydrocast data was processed to1-m bins. Prior to the cruise, the transmissometer was not calibrated and many data anomalies were observed; therefore, these data are suspect and not reported with the bottle data. </p>
<p>Samples were collected in the euphotic zone, or just below, to a maximum of ~60 m. Water was filtered (0.6 um pore size) for dissolved inorganic nutrient analysis (N+N, SRP, Si(OH)4), and analyzed at the University of California Santa Barbara Marine Science Institute’s Analytical Laboratory using flow injection techniques on a QuickChem 8000 analyzer (Lachat Instruments Division, Zellweger Analytics) as described in Brzezinski and Washburn (2011). Chlorophyll a was also quantified using the protocol followed in Brzezinski and Washburn (2011). ~175 – 350 mL of seawater was filtered through a 0.45 um-pore 47mm diameter HAWP Millipore filter, immediately frozen until analysis on shore (<2 weeks) via extracting with 90% acetone for 24 hours at -20C and quantification of fluorescence on a calibrated Turner Designs 10AU fluorometer with and without acidification. For biogenic silica analysis, 630 mL of seawater was filtered through a 1.2 um-pore polycarbonate filter (47 mm diameter) and frozen immediately. On shore, filters were dried and analyzed using a NaOH digestion in Teflon tubes which provide low and stable blank values (Krause et al., 2009). </p>
<p>For the cruise, three kinetic experiments were set up by filling eight 250-mL bottles and adding increasing enrichments of Si(OH)4 (to create a gradient from ambient to +20 uM). The rate of biogenic silica production in both size classes was measured using the radioisotope tracer 32Si with high specific activity (>40 kBq µmol/Si) as described in Krause et al. (2012). After incubation, samples were processed immediately as described in Krause et al. (2011). For PDMPO uptake, 48 uL of dye was added to each 250 mL bottle (e.g. 12-hr, 24-hr, ambient or enriched Si(OH)4 ) and incubated in the same conditions as the 32Si samples. After incubation, samples were processed as in McNair et al. (2015) to quantify the bulk PDMPO uptake. </p>
<p>Note: no ionophore rinse was used during the DYEatom cruise, this was added later in the McNair et al. (2015) protocol.</p></div>
Total silicic acid, gross silica production, and biomass-normalized measurements
<div><p>This dataset includes kinetic experiments, using 32Si and PDMPO, to determine the degree of Si uptake limitation by ambient silicic acid. The data include total silicic acid, gross silica production, and biomass-normalized measurements, concentrations of phosphate, nitrate+nitritie, total chlorophyll, chlorophyll a, phaeopigments, and CTD measurements (e.g. temperature, salinity).</p>
<p>PDMPO = 2-(4-pyridyl)-5-((4-(2-dimethylaminoethylaminocarbamoyl)methoxy)phenyl)oxazole</p></div>
Kinetics of Silicon uptake
<div><p>BCO-DMO Data Manager Processing Notes:<br />
* added a conventional header with dataset name, PI name, version date<br />
* modified parameter names to conform with BCO-DMO naming conventions<br />
* blank values replaced with no data value 'nd'<br />
* added minus sign to longitudes<br />
* -999 changed to "nd"<br />
* SEQ_NUM(CAST) added so it can be matched with CTD bottle and profile data<br />
* added ISO_DateTime_UTC<br />
* changed cruise value from DYEatom to PS13-12 to be consistent with CTD files<br />
* params starting 32Si changed to Si32 due to unsupported names with leading number.<br />
* data version 2018-03-12 replaces version 2017-08-17. In data version 2018-03-12 the following data parameters were added: Total_SiOH4, Si32_Rho, Si32_Vb, PDMPO_Rho</p></div>
701575
Kinetics of Silicon uptake
2017-05-26T15:50:30-04:00
2017-05-26T15:50:30-04:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:701575
Silicon uptake rates from samples collected during the R/V Point Sur cruise PS1312 along the Central California coast from June to July of 2013 (Diatom Group Si Prod project)
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Krause, J. W., Brzezinski, M. A. (2018) Silicon uptake rates from samples collected during the R/V Point Sur cruise PS1312 along the Central California coast from June to July of 2013 (Diatom Group Si Prod project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2018-03-12 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/701575 [access date]
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