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            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset/701579.rdf" xlink:actuate="onRequest">Silica production rates and concentrations quantified using 32Si and PDMPO using samples from the R/V Point Sur cruise PS1312 along the Central California coast from June to July of 2013 (Diatom Group Si Prod project)</gmx:Anchor>
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            <gco:CharacterString>Cite this dataset as: Krause, J. W., Brzezinski, M. A. (2018) Silica production rates and concentrations quantified using 32Si and PDMPO using samples from the R/V Point Sur cruise PS1312 along the Central California coast from June to July of 2013 (Diatom Group Si Prod project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2018-03-12 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/701579 [access date]</gco:CharacterString>
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        <gco:CharacterString>Silica production rates and concentrations quantified using 32Si and PDMPO Dataset Description: &amp;lt;p&amp;gt;This dataset includes diatom silica production rates, and concentrations quantified using 32Si and PDMPO (see below) tracers. Gross and biomass-normalized silica measurements from 12 hour and 24 hour incubations are provided as well as&amp;amp;nbsp;concentrations of phosphate, nitrate+nitritie, total chlorophyll, chlorophyll a, phaeopigments, and CTD measurements (e.g. temperature, salinity).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;PDMPO = 2-(4-pyridyl)-5-((4-(2-dimethylaminoethylaminocarbamoyl)methoxy)phenyl)oxazole&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;p&amp;gt;Multiple hydrocasts conducted at stations denoted in the bottle file meta data. &amp;amp;nbsp;A SeaBird CTD was used with redundant sensors for temperature and conductivity; the instrument package was owned and operated by the R/V Point Sur (Moss Landing Marine Laboratories, Moss Landing, California). &amp;amp;nbsp;All hydrocast data was processed to1-m bins. &amp;amp;nbsp;Prior to the cruise, the transmissometer was not calibrated and many data anomalies were observed; therefore, these data are suspect and not reported with the bottle data.&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Samples were collected in the euphotic zone, or just below, to a maximum of ~60 m. &amp;amp;nbsp;Water was filtered (0.6 um pore size) for dissolved inorganic nutrient analysis (N+N, SRP, Si(OH)4), and analyzed at the University of California Santa Barbara Marine Science Institute’s Analytical Laboratory using flow injection techniques on a QuickChem 8000 analyzer (Lachat Instruments Division, Zellweger Analytics) as described in Brzezinski and Washburn (2011). &amp;amp;nbsp;Chlorophyll a was also quantified using the protocol followed in Brzezinski and Washburn (2011). &amp;amp;nbsp;~175 – 350 mL of seawater was filtered through a 0.45 um-pore 47mm diameter HAWP Millipore filter, immediately frozen until analysis on shore (&amp;amp;lt;2 weeks) via extracting with 90% acetone for 24 hours at -20C and quantification of fluorescence on a calibrated Turner Designs 10AU fluorometer with and without acidification. &amp;amp;nbsp;For biogenic silica analysis, 630 mL of seawater was filtered through a 1.2 um-pore polycarbonate filter (47 mm diameter) and frozen immediately. &amp;amp;nbsp;On shore, filters were dried and analyzed using a NaOH digestion in Teflon tubes which provide low and stable blank values (Krause et al., 2009).&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Diatom rate processes were quantified using 32Si and PDMPO tracers. &amp;amp;nbsp;Samples were incubated in acrylic incubators cooled with continually flowing surface water under a series of neutral density screens to simulate light levels at the depth of collection. &amp;amp;nbsp;Four 250-mL bottle replicates were taken for each measurement, two for a 12-hour incubation (in daylight) and two for a 24-hour incubation; Si(OH)4 one replicate for each incubation duration was amended by +20 uM (i.e. Enrichment or “E” samples). &amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Note: no ionophore rinse was used during the DYEatom cruise, this was added later in the McNair et al. (2015) protocol.&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/550824.rdf" xlink:title="OCE-1155663" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1155663 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1155663</gmx:Anchor>
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                  <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/project/550825.rdf" xlink:title="Project" xlink:actuate="onRequest">Group-Specific Diatom Silica Production in a Coastal Upwelling System</gmx:Anchor>
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                            <gco:CharacterString>&lt;p&gt;This study will examine the distribution of silica production among diatom species, using a novel combination of existing approaches, to evaluate the contributions of specific species or genera to total diatom silica production. Specific hypotheses regarding the distribution of silica production among species of diatoms will be tested by exploiting the strong contrasts in diatom community structure and silica production between a coastal upwelling system and in an oligotrophic subtropical gyre. Several lines of evidence support the idea that the diatoms responsible for the majority of silica production shifts from the most numerically abundant species in coastal systems to relatively rare, but very large, cells in offshore oligotrophic environments. This shift alters the role of diatoms in regional food webs and because many processes determining the role of phytoplankton groups in biogeochemical cycles are a function of cell size, such a shift has strong implications for regional differences in the contribution of diatoms to upper-ocean carbon cycling and the biological pump. &lt;/p&gt;
&lt;p&gt;This study also seeks to understand of the role of silicon limitation in regulating diatom silica production at the species level. Si limitation of silica production has been detected in every system examined to date, ranging from the high Si waters of the Antarctic, to coastal upwelling systems and the oligotrophic subtropical gyres. Field studies of Si limitation are rarely accompanied by examination of the species present. When studies do have taxonomic data the lack of information on the performance of individual species makes it impossible to allocate the measured rates among cells, potentially leading to erroneous conclusions about the contribution of specific diatom groups to community composite rates.&lt;/p&gt;
&lt;p&gt;The project will test five hypotheses. Each is related to the general theme of using species-specific data to improve understanding of the factors regulating diatoms' role in marine food webs. By combining bulk measures of silica production using the radioisotope 32Si with quantitative measures of silicon deposition rates by individual cells using the fluorescent probe 2-(4-pyridyl)-5((4-(2-dimethylaminoethyl-aminocarbamoyl)-methoxy)phenyl)oxazole, or PDMPO, the following will be determined: species-specific diatom contributions to total community silica production, regional differences in the distribution of silica production among diatom species as a function of cell size, species-specific kinetic parameters governing the ability of species to compete for dissolved silicon, and whether dominance of a particular diatom group or species can be explained by knowledge of their capacity to utilize Si and their numerical abundance (as opposed to other factors such as grazing or limitation by other nutrients).&lt;/p&gt;</gco:CharacterString>
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                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/project/550825.rdf" xlink:title="Project Acronym" xlink:actuate="onRequest">Diatom Group Si Prod</gmx:Anchor>
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        <gmd:MD_TopicCategoryCode>oceans</gmd:MD_TopicCategoryCode>
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            <gco:CharacterString>Central California Coast</gco:CharacterString>
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                <gco:Decimal>-123.969</gco:Decimal>
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              <gmd:eastBoundLongitude>
                <gco:Decimal>-121.981</gco:Decimal>
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              <gmd:southBoundLatitude>
                <gco:Decimal>36.455</gco:Decimal>
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              <gmd:northBoundLatitude>
                <gco:Decimal>38.265</gco:Decimal>
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                  <gml:beginPosition>2013-06-27</gml:beginPosition>
                  <gml:endPosition>2013-07-05</gml:endPosition>
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                      <gco:CharacterString>In-, and off-shore, between Monterey Bay and Bodega Bay, CA</gco:CharacterString>
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            <gco:CharacterString>BCO-DMO catalogue of parameters from Silica production rates and concentrations quantified using 32Si and PDMPO using samples from the R/V Point Sur cruise PS1312 along the Central California coast from June to July of 2013 (Diatom Group Si Prod project)</gco:CharacterString>
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    <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/affiliation/191.rdf" xlink:actuate="onRequest">Biological and Chemical Oceanography Data Management Office (BCO-DMO)</gmx:Anchor>
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				    <gco:CharacterString>508-289-2009</gco:CharacterString>
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            http://lod.bco-dmo.org/id/dataset-parameter/730735.rdf
	Name: CAST_NUM
	Units: unitless
	Description: &lt;p&gt;CTD cast number&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730736.rdf
	Name: Cruise
	Units: unitless
	Description: &lt;p&gt;Cruise identifier&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730737.rdf
	Name: Latitude
	Units: unitless
	Description: &lt;p&gt;Latitude in decimal degrees&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730738.rdf
	Name: Longitude
	Units: unitless
	Description: &lt;p&gt;Longitude in decimal degrees&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730739.rdf
	Name: Event_num
	Units: unitless
	Description: &lt;p&gt;Event Number (YYYYMMDDHHHH; Zulu)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730740.rdf
	Name: Activity
	Units: unitless
	Description: &lt;p&gt;CTD number&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730741.rdf
	Name: Date_Local
	Units: unitless
	Description: &lt;p&gt;Date (local) in format dd-mmm-yy&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730742.rdf
	Name: Date_Zulu
	Units: unitless
	Description: &lt;p&gt;Time (local) in format hh:mm&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730743.rdf
	Name: Time_Local
	Units: unitless
	Description: &lt;p&gt;Date (UTC) in format dd-mmm-yy&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730744.rdf
	Name: Time_Zulu
	Units: unitless
	Description: &lt;p&gt;Time (UTC) in format hh:mm&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730745.rdf
	Name: ISO_DateTime_UTC
	Units: unitless
	Description: &lt;p&gt;ISO timestamp based on the ISO 8601:2004(E) standard in format YYYY-mm-ddTHH:MMZ (UTC)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730746.rdf
	Name: Station
	Units: unitless
	Description: &lt;p&gt;Station name&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730747.rdf
	Name: Bottom_Depth
	Units: meters
	Description: &lt;p&gt;Bottom depth&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730748.rdf
	Name: Actual_Depth
	Units: meters
	Description: &lt;p&gt;Depth of sample. Mid-point of niskin bottle at depth of collection (Depth sensor minus 0.5 m)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730749.rdf
	Name: Bottle_num
	Units: unitless
	Description: &lt;p&gt;Bottle number&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730750.rdf
	Name: Irradiance_percent
	Units: percent (%)
	Description: &lt;p&gt;Percentage of irradiance; measured just below the surface&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730751.rdf
	Name: Phosphate
	Units: micromoles per liter (umol/L)
	Description: &lt;p&gt;Autoanalyzer soluble reactive phosphorus&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730752.rdf
	Name: Nitrate_plus_Nitrite
	Units: micromoles per liter (umol/L)
	Description: &lt;p&gt;Autoanalyzer nitrate+nitrite&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730753.rdf
	Name: total_chl
	Units: micrograms per liter (ug/L)
	Description: &lt;p&gt;Total chlorophyll&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730754.rdf
	Name: chl_a
	Units: micrograms per liter (ug/L)
	Description: &lt;p&gt;Chlorophyll a&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730755.rdf
	Name: phaeo
	Units: micrograms per liter (ug/L)
	Description: &lt;p&gt;Phaeopigment&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730756.rdf
	Name: Total_SiOH4
	Units: micromoles per liter (umol/L)
	Description: &lt;p&gt;Manual dissolved silicic acid (Si(OH)4) concentration&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730757.rdf
	Name: bSiO2
	Units: micromoles of silicon per liter (umol Si/L)
	Description: &lt;p&gt;Biogenic silica (µmol Si/L)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730758.rdf
	Name: Si32_Rho_12hr
	Units: micromoles of silicon per liter per day (umol Si/L/d)
	Description: &lt;p&gt;12-hr incubation, Silicon-32-measured Gross biogenic silica production (Greek letter Rho)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730759.rdf
	Name: Si32_Vb_12hr
	Units: per day (d-1)
	Description: &lt;p&gt;12-hr incubation, Silicon-32-measured Biomass-normalized biogenic silica production (Vb)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730760.rdf
	Name: Si32_RhoE_12hr
	Units: micromoles of silicon per liter per day (umol Si/L/d)
	Description: &lt;p&gt;12-hr incubation, Silicon-32-measured Gross biogenic silica production +20µmol Si/L concentration (Greek letter RhoE)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730761.rdf
	Name: Si32_VbE_12hr
	Units: per day (d-1)
	Description: &lt;p&gt;12-hr incubation, Silicon-32-measured Biomass-normalized biogenic silica production +20µmol Si/L concentration (VbE)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730762.rdf
	Name: Si32_Rho_24hr
	Units: micromoles of silicon per liter per day (umol Si/L/d)
	Description: &lt;p&gt;24-hr incubation, Silicon-32-measured Gross biogenic silica production (Greek letter Rho)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730763.rdf
	Name: Si32_Vb_24hr
	Units: per day (d-1)
	Description: &lt;p&gt;24-hr incubation, Silicon-32-measured Biomass-normalized biogenic silica production (Vb)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730764.rdf
	Name: Si32_RhoE_24hr
	Units: micromoles of silicon per liter per day (umol Si/L/d)
	Description: &lt;p&gt;24-hr incubation, Silicon-32-measured Gross biogenic silica production +20µmol Si/L concentration (Greek letter RhoE)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730765.rdf
	Name: Si32_VbE_24hr
	Units: per day (d-1)
	Description: &lt;p&gt;24-hr incubation, Silicon-32-measured Biomass-normalized biogenic silica production +20µmol Si/L concentration (VbE)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730766.rdf
	Name: PDMPO_Rho_12hr
	Units: nanomoles of PDMPO per liter per day (nmol PDMPO/L/d)
	Description: &lt;p&gt;12-hr incubation, PDMPO-measured Gross biogenic silica production (Greek letter Rho)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730767.rdf
	Name: PDMPO_RhoE_12hr
	Units: nanomoles of PDMPO per liter per day (nmol PDMPO/L/d)
	Description: &lt;p&gt;12-hr incubation, PDMPO-measured Gross biogenic silica production +20µmol Si/L concentration (Greek letter RhoE)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730768.rdf
	Name: PDMPO_Rho_24hr
	Units: nanomoles of PDMPO per liter per day (nmol PDMPO/L/d)
	Description: &lt;p&gt;24-hr incubation, PDMPO-measured Gross biogenic silica production (Greek letter Rho)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730769.rdf
	Name: PDMPO_RhoE_24hr
	Units: nanomoles of PDMPO per liter per day (nmol PDMPO/L/d)
	Description: &lt;p&gt;24-hr incubation, PDMPO-measured Gross biogenic silica production +20µmol Si/L concentration (Greek letter RhoE)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730770.rdf
	Name: Temperature0
	Units: degrees Celsius
	Description: &lt;p&gt;Primary temperature from CTD (Seasave parameter T090C)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730771.rdf
	Name: Temperature1
	Units: degrees Celsius
	Description: &lt;p&gt;Secondary temperature from CTD (Seasave parameter T190C)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730772.rdf
	Name: Conductivity0
	Units: Siemens per meter (S/m)
	Description: &lt;p&gt;Primary conductivity from CTD (Seasave parameter C0S/m)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730773.rdf
	Name: Conductivity1
	Units: Siemens per meter (S/m)
	Description: &lt;p&gt;Secondary conductivity from CTD (Seasave parameter C1S/m)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730774.rdf
	Name: Salinity0
	Units: Practical Salinity Units (PSU)
	Description: &lt;p&gt;Primary salinity from CTD (Seasave parameter Sal00)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730775.rdf
	Name: Salinity1
	Units: Practical Salinity Units (PSU)
	Description: &lt;p&gt;Secondary sailinity from CTD (Seasave parameter Sal11)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730776.rdf
	Name: Density0
	Units: kilograms per meter cubed (kg/m3)
	Description: &lt;p&gt;Primary sigma-theta from CTD (Seasave parameter Sigma/e00)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730777.rdf
	Name: Density1
	Units: kilograms per meter cubed (kg/m3)
	Description: &lt;p&gt;Primary sigma-theta from CTD (Seasave parameter Sigma/e11)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730778.rdf
	Name: Fluorescence
	Units: milligrams per meter cubed (mg/m3)
	Description: &lt;p&gt;Fluorescence from Wet Labs ECO-AFL sensor on CTD (Seasave parameter FlECO/AFL)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730779.rdf
	Name: SBE_Oxygen
	Units: micromoles per kilogram (umol/kg)
	Description: &lt;p&gt;Dissolved Oxygen from CTD (Seasave parameter Sbeox0Mm/Kg)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730780.rdf
	Name: PAR
	Units: microeinsteins per square meter per second (uE/m2/s)
	Description: &lt;p&gt;Photosynthetically Active Radiation (PAR) from CTD (Seasave parameter Par)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730781.rdf
	Name: Surface_PAR
	Units: microeinsteins per square meter per second (uE/m2/s)
	Description: &lt;p&gt;Surface Photosynthetically Active Radiation (PAR) from CTD (Seasave parameter Spar)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/730782.rdf
	Name: CTD_file
	Units: unitless
	Description: &lt;p&gt;File name of the CTD profile data (.asc) file associated with this sample (contains cast number in name)&lt;/p&gt; 
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&amp;lt;p&amp;gt;Samples were collected in the euphotic zone, or just below, to a maximum of ~60 m. &amp;amp;nbsp;Water was filtered (0.6 um pore size) for dissolved inorganic nutrient analysis (N+N, SRP, Si(OH)4), and analyzed at the University of California Santa Barbara Marine Science Institute’s Analytical Laboratory using flow injection techniques on a QuickChem 8000 analyzer (Lachat Instruments Division, Zellweger Analytics) as described in Brzezinski and Washburn (2011). &amp;amp;nbsp;Chlorophyll a was also quantified using the protocol followed in Brzezinski and Washburn (2011). &amp;amp;nbsp;~175 – 350 mL of seawater was filtered through a 0.45 um-pore 47mm diameter HAWP Millipore filter, immediately frozen until analysis on shore (&amp;amp;lt;2 weeks) via extracting with 90% acetone for 24 hours at -20C and quantification of fluorescence on a calibrated Turner Designs 10AU fluorometer with and without acidification. &amp;amp;nbsp;For biogenic silica analysis, 630 mL of seawater was filtered through a 1.2 um-pore polycarbonate filter (47 mm diameter) and frozen immediately. &amp;amp;nbsp;On shore, filters were dried and analyzed using a NaOH digestion in Teflon tubes which provide low and stable blank values (Krause et al., 2009).&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Diatom rate processes were quantified using 32Si and PDMPO tracers. &amp;amp;nbsp;Samples were incubated in acrylic incubators cooled with continually flowing surface water under a series of neutral density screens to simulate light levels at the depth of collection. &amp;amp;nbsp;Four 250-mL bottle replicates were taken for each measurement, two for a 12-hour incubation (in daylight) and two for a 24-hour incubation; Si(OH)4 one replicate for each incubation duration was amended by +20 uM (i.e. Enrichment or “E” samples). &amp;amp;nbsp;&amp;lt;/p&amp;gt;

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* Dataset version 2018-03-12 replaces version&amp;amp;nbsp;2017-08-17.&amp;amp;nbsp; In version 2018-03-12 the following data parameters were added:&amp;amp;nbsp;Total_SiOH4,bSiO2,Si32_Rho_12hr,Si32_Vb_12hr,Si32_RhoE_12hr,Si32_VbE_12hr,Si32_Rho_24hr,Si32_Vb_24hr,Si32_RhoE_24hr,Si32_VbE_24hr,PDMPO_Rho_12hr,PDMPO_RhoE_12hr,PDMPO_Rho_24hr,PDMPO_RhoE_24hr&amp;lt;/p&amp;gt;</gco:CharacterString>
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