http://lod.bco-dmo.org/id/dataset/701885
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2017-06-02
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Dissolved organic carbon and amino acid data from the NBP1207 cruise in Chile during 2012
2017-06-02
publication
2017-06-02
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2021-05-28
publication
https://doi.org/10.26008/1912/bco-dmo.701885.1
Ronald Benner
University of South Carolina at Columbia
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Benner, R. (2021) Dissolved organic carbon and amino acid data from the NBP1207 cruise in Chile during 2012. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2017-06-02 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.701885.1 [access date]
Dissolved organic carbon and amino acid data from NBP1207. Dataset Description: <pre>
Dissolved organic carbon and amino acid data from NBP1207.
</pre> Methods and Sampling: <p>Methodology from&nbsp;<strong>Shen, Y., et al. (2017), Bioavailable dissolved organic matter and biological hot spots during austral winter in Antarctic waters, J. Geophys. Res. Oceans, 121, <a href="http://onlinelibrary.wiley.com/doi/10.1002/2016JC012301/abstract" target="_blank">doi:10.1002/2016JC012301</a>.</strong></p>
<p><strong>Study Area and Sample Collection</strong></p>
<p>Sampling was conducted as part of the Antarctic Marine Living Resources (AMLR) Program aboard the RVIB Nathaniel B. Palmer off the Antarctic Peninsula and South Shetland Islands (SSI) during August 2012 (Figure 1). The sampling areas are characterized by the confluence of different water masses originating from the Antarctic Circumpolar Current, Bellingshausen Sea, and Weddell Sea. A total of 110 seawater samples were collected from discrete depths (5, 10, 15, 50, 75, 100, 200, 750 m) at 25 stations within the historic AMLR research area, extending from the southern Drake Passage to the Bransfield Strait (Figure 1). A rosette sampler system with 24, 12 L bottles and a Seabird Conductivity-Temperature-Depth (CTD) instrument was used for water collections. Water samples for chlorophyll-a (chl-a) were filtered (GF/F; 0.7 mm pore size; Whatman) immediately following collection. Samples for POC measurements were collected mostly at 5 and 200 m, filtered (GF/F; Whatman; precombusted at 4508C for 5 h), and stored frozen until analysis. Water samples for analyses of DOC and amino acids were stored frozen (2808C) in 60 mL high density polyethylene screw-cap bottles immediately after collection. Hydrographic data were obtained&nbsp;from the CTD sensors and were used to determine the depth of upper mixed layer and to identify water masses (Table 1).</p>
<p><strong>Chemical Analyses</strong></p>
<p>The filters for chl-a determinations were extracted in 7 mL of methanol for 24 h, centrifuged, and measured for fluorescence using an acidification module in a Turner Trilogy fluorometer. Readings were calibrated with a 5-point calibration curve using a chl-a standard obtained from Sigma, the concentration of which was determined using a Lambda-18 spectrophotometer. Samples (GF/F filters) for POC analysis were treated with 10% v/v hydrochloric acid (HCl), dried at 608C, and analyzed using an Exeter Analytical CEC 440HA elemental analyzer.</p>
<p>Water samples for DOC and amino acid measurements were filtered through 0.2 mm pore size membranes (SuporVR -200, Life Sciences). The Supor membranes were cleaned with methanol and then rinsed thoroughly with Milli-Q UV-Plus water before use. The DOC samples were acidified to pH 2–3 with 2 mol L21 HCl. Concentrations of DOC were determined by high-temperature combustion using a Shimadzu total organic carbon TOC-V analyzer equipped with an autosampler. Milli-Q UV-Plus water and seawater reference standards were injected every sixth sample [Benner and Strom, 1993]. Blanks (Milli-Q water) were negligible and the measured concentrations of reference standards were within the reported range (41–44 mmol L21). The coefficient of variation among four injections of a given DOC sample was typically 61.1%.</p>
<p>The D-enantiomer and L-enantiomer of amino acids were analyzed using an Agilent 1260 ultrahigh-performance liquid chromatography (UPLC) system equipped with a fluorescence detector (excitation: 330 nm; &nbsp;emission: 450 nm) [Shen et al., 2015]. Amino acids were determined in all samples that were filtered through Supor membranes (0.2 mm pore size) and in a subset of unfiltered samples as total dissolved amino acids (TDAA) and total particulate amino acids (TPAA), respectively. Hydrolysis and derivatization followed the procedures described by Kaiser and Benner [2005]. Briefly, water samples (100 mL) were dried and hydrolyzed using a vapor-phase technique with 6 mol L21 HCl at 1508C for 32.5 min. Amino acid enantiomers were derivatized with o-phthaldialdehyde and N-isobutyryl-L-cysteine and were separated on a Poroshell 120 EC-C18 (4.6 3 100 mm, 2.7 mm particles) column. A linear binary gradient was used starting with 100% potassium di-hydrogen phosphate (KH2PO4; 48 mmol L21, pH56.25) to 61% KH2PO4 and 39% methanol:acetonitrile (13:1, v/v) at 13.3 min, 46% KH2PO4 at 19.2 min, 40% KH2PO4 at 21.3 min, and 20% KH2PO4 at 22 min. Eighteen amino acids were included in the analysis: asparagine1aspartic acid (Asx), glutamine1glutamic acid (Glx), serine (Ser), histidine (His), glycine (Gly), threonine (Thr), b-alanine (b-Ala), arginine (Arg), alanine (Ala), c-aminobutyric acid (c-Aba), tyrosine (Tyr), valine (Val), phenylalanine (Phe), isoleucine (Ile), leucine (Leu), and lysine (Lys). Acid-catalyzed racemization was corrected according to Kaiser and Benner [2005]. This method has a limit of quantification of 0.5 nmol L21 for individual amino acids. D-enantiomers of Asx, Glx, Ser, and Ala are reported in this study.</p>
<p>Concentrations of TDAA and TPAA were determined as the total concentrations of the eighteen dissolved and particulate amino acids, respectively. DOC-normalized yields of TDAA were calculated as the percentage contributions of amino acid carbon to the total DOC, using equation (1) and being reported in units of %DOC:</p>
<p>TDAA (%DOC) = ( [TDAA-C] / [DOC] ) x 100</p>
<p>where [DOC] and [TDAA-C] are the concentrations of bulk DOC and carbon measured in the total dissolved amino acids, respectively. This calculation excluded the two nonprotein amino acids (b-Ala and c-Aba) that are thought to be byproducts of decomposition [Cowie and Hedges, 1994].</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1233373 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1233373
completed
Ronald Benner
University of South Carolina at Columbia
8037779561
715 Sumter Street Department of Biological Sciences
Columbia
SC
292080001
US
BENNER@mailbox.sc.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
sample_ID
date
time
lon
lat
potemp_C
salinity
depth
DOC_uM
TDN_uM
TDAA_nM_filtered
TDAA_pcentDOC_filtered
L_Asx_filtered
D_Asx_filtered
L_Glx_filtered
D_Glx_filtered
L_Ser_filtered
D_Ser_filtered
L_His_filtered
L_Thr_filtered
Gly_filtered
L_Arg_filtered
beta_Ala_filtered
L_Ala_filtered
gamma_Aba_filtered
D_Ala_filtered
L_Tyr_filtered
L_Val_filtered
L_Phe_filtered
L_Ileu_filtered
L_Leu_filtered
L_Lys_filtered
TAA_nM_unfiltered
TAA_unfiltered
L_Asx_unfiltered
D_Asx_unfiltered
L_Glx_unfiltered
D_Glx_unfiltered
L_Ser_unfiltered
D_Ser_unfiltered
L_His_unfiltered
L_Thr_unfiltered
Gly_unfiltered
L_Arg_unfiltered
beta_Ala_unfiltered
L_Ala_unfiltered
gamma_Aba_unfiltered
D_Ala_unfiltered
L_Tyr_unfiltered
L_Val_unfiltered
L_Phe_unfiltered
L_Ileu_unfiltered
L_Leu_unfiltered
L_Lys_unfiltered
ISO_DateTime_UTC
Seabird Conductivity-Temperature-Depth instrument
Turner Trilogy Fluorometer
Shimadzu total organic carbon TOC-V analyzer
theme
None, User defined
sample identification
date
time of day
longitude
latitude
water temperature at measurement depth
salinity
depth
dissolved organic Carbon
Total Dissolved Nitrogen
amino acid concentration
total organic Carbon
ISO_DateTime_UTC
featureType
BCO-DMO Standard Parameters
CTD Sea-Bird
Fluorometer
Total Organic Carbon Analyzer
instrument
BCO-DMO Standard Instruments
NBP1207
service
Deployment Activity
Punta Arenas, Chile
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
US Antarctic Marine Living Resources Program
https://osprey.bco-dmo.org/program/679451
US Antarctic Marine Living Resources Program
Since 1986, the NOAA Antarctic Ecosystems Research Division (AERD) has managed the U.S. AMLR Program's field studies in Antarctic waters to investigate the effects of commercial fisheries on the marine ecosystem, including effects on local seal and seabird populations. Studies conducted by U.S. AMLR/AERD researchers include: Annual research vessel survey to map prey distribution and abundance, and to measure environmental variables in a study area off the Antarctic Peninsula; Research at land stations to determine effects of fishing on pinniped and seabird populations during their reproductive cycles; and Accounting for the status and role of mesopelagic species such as myctophids, Pleurogramma, etc.
Additional research and activities conducted by the AERD in support of the AMLR Program's objectives include: Monitoring commercial fishing vessels to determine catch statistics, by‑catch amount and composition, and occurrence of marine mammal and seabird interactions; Conducting research on the improvement of field methodologies, effects of instrumentation, degree of data biases, and occurrence of sampling errors; Collaborate with international partners, including participation in their field programs; and Providing leadership and advice to CCAMLR's Commission and Scientific Committee and its Working Groups.
AMLR
largerWorkCitation
program
The Microbial Carbon Pump and Bacterial Carbon Sequestration in the Ocean
https://osprey.bco-dmo.org/project/679585
The Microbial Carbon Pump and Bacterial Carbon Sequestration in the Ocean
<p>NSF Award Abstract:</p>
<p>The Biological Pump has long been recognized as an important sink for atmospheric CO2 concentrations through the sequestration of carbon in ocean sediments. The contributions of bacteria to the sequestration of carbon in the ocean reservoir of refractory dissolved organic carbon (RDOC) have only recently been recognized. This process, conceptualized as the Microbial Carbon Pump (MCP), also appears to play a significant role in carbon sequestration and the regulation of atmospheric CO2.</p>
<p>In this project, researchers at the University of South Carolina at Columbia will build upon the framework of the MCP to address the following hypotheses: 1) The production and composition of bacterial DOC vary with biological (e.g. community structure) and physicochemical (e.g. nutrients) parameters in the ocean, 2) Bacteria sequester carbon in semi-labile DOC (SDOC) that persists for years to decades in mesopelagic waters, 3) Microbial communities in mesopelagic and bathypelagic waters have the metabolic potential to produce and degrade bacterial DOC. Naturally-occurring bacterial DOC in these waters is very resistant to microbial degradation, 4) Heterotrophic bacteria are the predominant source of bacterial SDOC and RDOC. These hypotheses will be addressed using a combination of field (NW Pacific Ocean and Sargasso Sea) and experimental approaches to investigate spatial variability in the production and decomposition of bacterial DOC and to relate these processes to community structure and environmental conditions. This study will provide estimates of the reservoirs of bacterial SDOC and RDOC in the ocean and critical insights about the ocean carbon cycle and the regulation of atmospheric CO2 concentrations. It will also lay a foundation for addressing how the MCP and bacterial carbon sequestration will respond to climate change.</p>
<p>Broader Impacts: This project will strengthen collaborations between the PI's laboratory and Dr. Hiroshi Ogawa's laboratory at the Atmosphere and Ocean Research Institute in Japan. This collaboration includes participation on a Japanese-sponsored expedition to the North Pacific Ocean aboard the R/V Hakuro maru. Additionally, it will support a postdoctoral associate and a doctoral student who will participate on research expeditions to the NW Pacific Ocean and Sargasso Sea. Their research will focus on the MCP, and they will present their findings at national conferences in years 2 and 3.</p>
MCP
largerWorkCitation
project
eng; USA
oceans
Punta Arenas, Chile
-62.5061
-57.5446
-60.0544
-53.9594
2012-08-06
2012-08-14
South Shetland Islands off the Antarctic Peninsula
0
BCO-DMO catalogue of parameters from Dissolved organic carbon and amino acid data from the NBP1207 cruise in Chile during 2012
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/701900.rdf
Name: sample_ID
Units: unitless
Description: <p>Sample ID</p>
http://lod.bco-dmo.org/id/dataset-parameter/701901.rdf
Name: date
Units: unitless
Description: <p>Sampling date; YYYY/MM/DD</p>
http://lod.bco-dmo.org/id/dataset-parameter/701902.rdf
Name: time
Units: unitless
Description: <p>Sampling time; HH:MM:SS</p>
http://lod.bco-dmo.org/id/dataset-parameter/701903.rdf
Name: lon
Units: decimal degrees
Description: <p>Longitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/701904.rdf
Name: lat
Units: decimal degrees
Description: <p>Latitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/701905.rdf
Name: potemp_C
Units: celsius
Description: <p>CTD Potential Temp sensor 90</p>
http://lod.bco-dmo.org/id/dataset-parameter/701906.rdf
Name: salinity
Units: PSU
Description: <p>CTD Salinity</p>
http://lod.bco-dmo.org/id/dataset-parameter/701907.rdf
Name: depth
Units: meters
Description: <p>Sampling depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/701908.rdf
Name: DOC_uM
Units: umol/L
Description: <p>Dissolved organic carbon</p>
http://lod.bco-dmo.org/id/dataset-parameter/701909.rdf
Name: TDN_uM
Units: umol/L
Description: <p>Total dissolved nitrogen</p>
http://lod.bco-dmo.org/id/dataset-parameter/701910.rdf
Name: TDAA_nM_filtered
Units: nmol/L
Description: <p>Total dissolved amino acids; Samples were filtered through Supor membranes (0.2-um pore size) before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701911.rdf
Name: TDAA_pcentDOC_filtered
Units: percent
Description: <p>Percentage of DOC that is measured as TDAA; Samples were filtered through Supor membranes (0.2-um pore size) before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701912.rdf
Name: L_Asx_filtered
Units: nmol/L
Description: <p>Dissolved L-asparagine + L-aspartic acid; Samples were filtered through Supor membranes (0.2-um pore size) before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701913.rdf
Name: D_Asx_filtered
Units: nmol/L
Description: <p>Dissolved D-asparagine + D-aspartic acid; Samples were filtered through Supor membranes (0.2-um pore size) before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701914.rdf
Name: L_Glx_filtered
Units: nmol/L
Description: <p>Dissolved L-glutamine + L-glutamic acid; Samples were filtered through Supor membranes (0.2-um pore size) before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701915.rdf
Name: D_Glx_filtered
Units: nmol/L
Description: <p>Dissolved D-glutamine + D-glutamic acid; Samples were filtered through Supor membranes (0.2-um pore size) before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701916.rdf
Name: L_Ser_filtered
Units: nmol/L
Description: <p>Dissolved L-serine; Samples were filtered through Supor membranes (0.2-um pore size) before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701917.rdf
Name: D_Ser_filtered
Units: nmol/L
Description: <p>Dissolved D-serine; Samples were filtered through Supor membranes (0.2-um pore size) before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701918.rdf
Name: L_His_filtered
Units: nmol/L
Description: <p>Dissolved L-histidine; Samples were filtered through Supor membranes (0.2-um pore size) before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701919.rdf
Name: L_Thr_filtered
Units: nmol/L
Description: <p>Dissolved L-threonine; Samples were filtered through Supor membranes (0.2-um pore size) before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701920.rdf
Name: Gly_filtered
Units: nmol/L
Description: <p>Dissolved glycine; Samples were filtered through Supor membranes (0.2-um pore size) before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701921.rdf
Name: L_Arg_filtered
Units: nmol/L
Description: <p>Dissolved L-arginine; Samples were filtered through Supor membranes (0.2-um pore size) before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701922.rdf
Name: beta_Ala_filtered
Units: nmol/L
Description: <p>Dissolved beta-alanine; Samples were filtered through Supor membranes (0.2-um pore size) before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701923.rdf
Name: L_Ala_filtered
Units: nmol/L
Description: <p>Dissolved L-alanine; Samples were filtered through Supor membranes (0.2-um pore size) before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701924.rdf
Name: gamma_Aba_filtered
Units: nmol/L
Description: <p>Dissolved gamma-aminobutyric acid; Samples were filtered through Supor membranes (0.2-um pore size) before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701925.rdf
Name: D_Ala_filtered
Units: nmol/L
Description: <p>Dissolved D-alanine; Samples were filtered through Supor membranes (0.2-um pore size) before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701926.rdf
Name: L_Tyr_filtered
Units: nmol/L
Description: <p>Dissolved L-tyrosine; Samples were filtered through Supor membranes (0.2-um pore size) before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701927.rdf
Name: L_Val_filtered
Units: nmol/L
Description: <p>Dissolved L-valine; Samples were filtered through Supor membranes (0.2-um pore size) before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701928.rdf
Name: L_Phe_filtered
Units: nmol/L
Description: <p>Dissolved L-phenylalanine; Samples were filtered through Supor membranes (0.2-um pore size) before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701929.rdf
Name: L_Ileu_filtered
Units: nmol/L
Description: <p>Dissloved L-isoleucine; Samples were filtered through Supor membranes (0.2-um pore size) before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701930.rdf
Name: L_Leu_filtered
Units: nmol/L
Description: <p>Dissolved L-leucine; Samples were filtered through Supor membranes (0.2-um pore size) before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701931.rdf
Name: L_Lys_filtered
Units: nmol/L
Description: <p>Dissolved L-lysine; Samples were filtered through Supor membranes (0.2-um pore size) before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701932.rdf
Name: TAA_nM_unfiltered
Units: nmol/L
Description: <p>Total amino acids (i.e. Total dissolved + total particulate amino acids); Samples were NOT filtered before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701933.rdf
Name: TAA_unfiltered
Units: nmol/L
Description: <p>Percentage of total organic carbon that is measured as TAA; Samples were NOT filtered before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701934.rdf
Name: L_Asx_unfiltered
Units: nmol/L
Description: <p>Total L-asparagine + L-aspartic acid; Samples were NOT filtered before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701935.rdf
Name: D_Asx_unfiltered
Units: nmol/L
Description: <p>Total D-asparagine + D-aspartic acid; Samples were NOT filtered before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701936.rdf
Name: L_Glx_unfiltered
Units: nmol/L
Description: <p>Total L-glutamine + L-glutamic acid; Samples were NOT filtered before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701937.rdf
Name: D_Glx_unfiltered
Units: nmol/L
Description: <p>Total D-glutamine + D-glutamic acid; Samples were NOT filtered before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701938.rdf
Name: L_Ser_unfiltered
Units: nmol/L
Description: <p>Total L-serine; Samples were NOT filtered before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701939.rdf
Name: D_Ser_unfiltered
Units: nmol/L
Description: <p>Total D-serine; Samples were NOT filtered before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701940.rdf
Name: L_His_unfiltered
Units: nmol/L
Description: <p>Total L-histidine; Samples were NOT filtered before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701941.rdf
Name: L_Thr_unfiltered
Units: nmol/L
Description: <p>Total L-threonine; Samples were NOT filtered before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701942.rdf
Name: Gly_unfiltered
Units: nmol/L
Description: <p>Total glycine; Samples were NOT filtered before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701943.rdf
Name: L_Arg_unfiltered
Units: nmol/L
Description: <p>Total L-arginine; Samples were NOT filtered before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701944.rdf
Name: beta_Ala_unfiltered
Units: nmol/L
Description: <p>Total beta-alanine; Samples were NOT filtered before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701945.rdf
Name: L_Ala_unfiltered
Units: nmol/L
Description: <p>Total L-alanine; Samples were NOT filtered before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701946.rdf
Name: gamma_Aba_unfiltered
Units: nmol/L
Description: <p>Total gamma-aminobutyric acid; Samples were NOT filtered before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701947.rdf
Name: D_Ala_unfiltered
Units: nmol/L
Description: <p>Total D-alanine; Samples were NOT filtered before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701948.rdf
Name: L_Tyr_unfiltered
Units: nmol/L
Description: <p>Total L-tyrosine; Samples were NOT filtered before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701949.rdf
Name: L_Val_unfiltered
Units: nmol/L
Description: <p>Total L-valine; Samples were NOT filtered before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701950.rdf
Name: L_Phe_unfiltered
Units: nmol/L
Description: <p>Total L-phenylalanine; Samples were NOT filtered before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701951.rdf
Name: L_Ileu_unfiltered
Units: nmol/L
Description: <p>Dissloved L-isoleucine; Samples were NOT filtered before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701952.rdf
Name: L_Leu_unfiltered
Units: nmol/L
Description: <p>Total L-leucine; Samples were NOT filtered before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701953.rdf
Name: L_Lys_unfiltered
Units: nmol/L
Description: <p>Total L-lysine; Samples were NOT filtered before amino acid analysis</p>
http://lod.bco-dmo.org/id/dataset-parameter/701954.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>Date/Time (UTC) ISO formatted</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
22340
https://darchive.mblwhoilibrary.org/bitstream/1912/27181/1/dataset-701885_dissolved-organic-carbon-and-amino-acids__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.701885.1
download
onLine
dataset
<p>Methodology from&nbsp;<strong>Shen, Y., et al. (2017), Bioavailable dissolved organic matter and biological hot spots during austral winter in Antarctic waters, J. Geophys. Res. Oceans, 121, <a href="http://onlinelibrary.wiley.com/doi/10.1002/2016JC012301/abstract" target="_blank">doi:10.1002/2016JC012301</a>.</strong></p>
<p><strong>Study Area and Sample Collection</strong></p>
<p>Sampling was conducted as part of the Antarctic Marine Living Resources (AMLR) Program aboard the RVIB Nathaniel B. Palmer off the Antarctic Peninsula and South Shetland Islands (SSI) during August 2012 (Figure 1). The sampling areas are characterized by the confluence of different water masses originating from the Antarctic Circumpolar Current, Bellingshausen Sea, and Weddell Sea. A total of 110 seawater samples were collected from discrete depths (5, 10, 15, 50, 75, 100, 200, 750 m) at 25 stations within the historic AMLR research area, extending from the southern Drake Passage to the Bransfield Strait (Figure 1). A rosette sampler system with 24, 12 L bottles and a Seabird Conductivity-Temperature-Depth (CTD) instrument was used for water collections. Water samples for chlorophyll-a (chl-a) were filtered (GF/F; 0.7 mm pore size; Whatman) immediately following collection. Samples for POC measurements were collected mostly at 5 and 200 m, filtered (GF/F; Whatman; precombusted at 4508C for 5 h), and stored frozen until analysis. Water samples for analyses of DOC and amino acids were stored frozen (2808C) in 60 mL high density polyethylene screw-cap bottles immediately after collection. Hydrographic data were obtained&nbsp;from the CTD sensors and were used to determine the depth of upper mixed layer and to identify water masses (Table 1).</p>
<p><strong>Chemical Analyses</strong></p>
<p>The filters for chl-a determinations were extracted in 7 mL of methanol for 24 h, centrifuged, and measured for fluorescence using an acidification module in a Turner Trilogy fluorometer. Readings were calibrated with a 5-point calibration curve using a chl-a standard obtained from Sigma, the concentration of which was determined using a Lambda-18 spectrophotometer. Samples (GF/F filters) for POC analysis were treated with 10% v/v hydrochloric acid (HCl), dried at 608C, and analyzed using an Exeter Analytical CEC 440HA elemental analyzer.</p>
<p>Water samples for DOC and amino acid measurements were filtered through 0.2 mm pore size membranes (SuporVR -200, Life Sciences). The Supor membranes were cleaned with methanol and then rinsed thoroughly with Milli-Q UV-Plus water before use. The DOC samples were acidified to pH 2–3 with 2 mol L21 HCl. Concentrations of DOC were determined by high-temperature combustion using a Shimadzu total organic carbon TOC-V analyzer equipped with an autosampler. Milli-Q UV-Plus water and seawater reference standards were injected every sixth sample [Benner and Strom, 1993]. Blanks (Milli-Q water) were negligible and the measured concentrations of reference standards were within the reported range (41–44 mmol L21). The coefficient of variation among four injections of a given DOC sample was typically 61.1%.</p>
<p>The D-enantiomer and L-enantiomer of amino acids were analyzed using an Agilent 1260 ultrahigh-performance liquid chromatography (UPLC) system equipped with a fluorescence detector (excitation: 330 nm; &nbsp;emission: 450 nm) [Shen et al., 2015]. Amino acids were determined in all samples that were filtered through Supor membranes (0.2 mm pore size) and in a subset of unfiltered samples as total dissolved amino acids (TDAA) and total particulate amino acids (TPAA), respectively. Hydrolysis and derivatization followed the procedures described by Kaiser and Benner [2005]. Briefly, water samples (100 mL) were dried and hydrolyzed using a vapor-phase technique with 6 mol L21 HCl at 1508C for 32.5 min. Amino acid enantiomers were derivatized with o-phthaldialdehyde and N-isobutyryl-L-cysteine and were separated on a Poroshell 120 EC-C18 (4.6 3 100 mm, 2.7 mm particles) column. A linear binary gradient was used starting with 100% potassium di-hydrogen phosphate (KH2PO4; 48 mmol L21, pH56.25) to 61% KH2PO4 and 39% methanol:acetonitrile (13:1, v/v) at 13.3 min, 46% KH2PO4 at 19.2 min, 40% KH2PO4 at 21.3 min, and 20% KH2PO4 at 22 min. Eighteen amino acids were included in the analysis: asparagine1aspartic acid (Asx), glutamine1glutamic acid (Glx), serine (Ser), histidine (His), glycine (Gly), threonine (Thr), b-alanine (b-Ala), arginine (Arg), alanine (Ala), c-aminobutyric acid (c-Aba), tyrosine (Tyr), valine (Val), phenylalanine (Phe), isoleucine (Ile), leucine (Leu), and lysine (Lys). Acid-catalyzed racemization was corrected according to Kaiser and Benner [2005]. This method has a limit of quantification of 0.5 nmol L21 for individual amino acids. D-enantiomers of Asx, Glx, Ser, and Ala are reported in this study.</p>
<p>Concentrations of TDAA and TPAA were determined as the total concentrations of the eighteen dissolved and particulate amino acids, respectively. DOC-normalized yields of TDAA were calculated as the percentage contributions of amino acid carbon to the total DOC, using equation (1) and being reported in units of %DOC:</p>
<p>TDAA (%DOC) = ( [TDAA-C] / [DOC] ) x 100</p>
<p>where [DOC] and [TDAA-C] are the concentrations of bulk DOC and carbon measured in the total dissolved amino acids, respectively. This calculation excluded the two nonprotein amino acids (b-Ala and c-Aba) that are thought to be byproducts of decomposition [Cowie and Hedges, 1994].</p>
Specified by the Principal Investigator(s)
<p>Processing description from&nbsp;<strong>Shen, Y., et al. (2017), Bioavailable dissolved organic matter and biological hot spots during austral winter in Antarctic waters, J. Geophys. Res. Oceans, 121,&nbsp;<a href="http://onlinelibrary.wiley.com/doi/10.1002/2016JC012301/abstract" target="_blank">doi:10.1002/2016JC012301</a>.</strong></p>
<p>The significance of least squares linear regression analyses between variables was determined using the enter approach in SPSS 20.0 (IBM Statistical Package for the Social Sciences Inc.). Normality of residuals was tested using a Kolmogorov-Smirnov test (two-tailed, a50.05). Statistical differences between variables were analyzed using the nonparametric Mann-Whitney U test (two-tailed, a50.05), which makes no assumptions of equal group size and normality of data distribution.</p>
<p><strong>BCO-DMO Data Processing Description:</strong></p>
<p>- reformatted column names to comply with BCO-DMO standards<br />
- filled all empty cells with "nd"<br />
- added ISO_DateTime_UTC column to meet community standards</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Seabird Conductivity-Temperature-Depth instrument
Seabird Conductivity-Temperature-Depth instrument
PI Supplied Instrument Name: Seabird Conductivity-Temperature-Depth instrument PI Supplied Instrument Description:Used for water collections Instrument Name: CTD Sea-Bird Instrument Short Name:CTD Sea-Bird Instrument Description: A Conductivity, Temperature, Depth (CTD) sensor package from SeaBird Electronics. This instrument designation is used when specific make and model are not known or when a more specific term is not available in the BCO-DMO vocabulary. Refer to the dataset-specific metadata for more information about the specific CTD used. More information from: http://www.seabird.com/ Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/130/
Turner Trilogy Fluorometer
Turner Trilogy Fluorometer
PI Supplied Instrument Name: Turner Trilogy Fluorometer PI Supplied Instrument Description:Used to measure fluorescence Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/
Shimadzu total organic carbon TOC-V analyzer
Shimadzu total organic carbon TOC-V analyzer
PI Supplied Instrument Name: Shimadzu total organic carbon TOC-V analyzer PI Supplied Instrument Description:Used to measure DOC Instrument Name: Total Organic Carbon Analyzer Instrument Short Name:TOC analyzer Instrument Description: A unit that accurately determines the carbon concentrations of organic compounds typically by detecting and measuring its combustion product (CO2). See description document at: http://bcodata.whoi.edu/LaurentianGreatLakes_Chemistry/bs116.pdf Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/
Cruise: NBP1207
NBP1207
Community Standard Description
International Council for the Exploration of the Sea
RVIB Nathaniel B. Palmer
vessel
NBP1207
George Watters
National Oceanic and Atmospheric Administration - Southwest Fisheries Science Center
Community Standard Description
International Council for the Exploration of the Sea
RVIB Nathaniel B. Palmer
vessel