http://lod.bco-dmo.org/id/dataset/709476
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2017-07-21
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Water column data from R/V Atlantic Explorer cruise AE1409 in the Western Tropical North Atlantic from May 2014 (P Processing by Tricho project)
2017-07-20
publication
2017-07-20
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2021-05-24
publication
https://doi.org/10.26008/1912/bco-dmo.709476.1
Benjamin A.S. Van Mooy
Woods Hole Oceanographic Institution
principalInvestigator
Sonya T. Dyhrman
Lamont-Doherty Earth Observatory
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Van Mooy, B. A., Dyhrman, S. T. (2021) Water column data from R/V Atlantic Explorer cruise AE1409 in the Western Tropical North Atlantic from May 2014 (P Processing by Tricho project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2017-07-20 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.709476.1 [access date]
Water column data from cruise AE1409 Dataset Description: <p>Water column data from cruise AE1409.</p> Methods and Sampling: <p>MAGIC Soluble reactive phosphorus (SRP) concentrations.&nbsp; SRP (i.e. phosphate) was determined in seawaters samples and incubations was quantified using MAGnesium Induced Coprecipitation (MAGIC) as described by Karl and Tien (1992).</p>
<p>Total dissolved phosphorus (TDP) concentrations – TDP was determined on 0.2 um filtrates of surface water (~5 m depth) samples collected via CTD into acid-clean polycarbonate bottles. Samples were processed at the SOEST Laboratory for Analytical Biogeochemistry at the University of Hawaii, according to facility protocols.</p>
<p>Alkaline phosphatase rates - Alkaline phosphatase activity samples were obtained by placing 2-5 cleaned <em>Trichodesmium </em>colonies on 5 um PC filters, gently vacuum filtering away excess liquid, then storing in 47 mm plastic petri dishes at -20 C until analysis. Samples were processed as previously described by Dyhrman and Ruttenberg (2006) using 6,8-difluoro-4-methylumbeliferyl phosphate (DiMufP) on a Synergy H1 Hybrid plate reader using the Gen5 software package (BioTek, Winooski, VT)</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1332898 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1332898
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1332912 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1332912
completed
Benjamin A.S. Van Mooy
Woods Hole Oceanographic Institution
(508) 289 2322
Department of Marine Chemistry and Geochemistry, MS #4, Fye 117 Woods Hole Oceanographic Institution
Woods Hole
MA
02543
USA
bvanmooy@whoi.edu
pointOfContact
Sonya T. Dyhrman
Lamont-Doherty Earth Observatory
845-365-8465
102E Geoscience 61 Route 9W, PO Box 1000
Palisades
NY
10964
USA
dyhrman@ldeo.columbia.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Date
Station
Depth
Sample
SRP
MAGIC_SRP
TDP
DOP_calc
Lat
Long
Niskin bottles
AA3 Nutrient Autoanalyzer
Synergy H1 Hybrid plate reader
temperature logger (Onset)
theme
None, User defined
date
station number
depth
sample identification
SRP
Total Dissolved Phosphorus
Dissolved Organic Phosphorus
latitude
longitude
featureType
BCO-DMO Standard Parameters
Niskin bottle
Nutrient Autoanalyzer
plate reader
Water Temperature Sensor
instrument
BCO-DMO Standard Instruments
AE1409
service
Deployment Activity
Western Tropical North Atlantic
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Dissolved Phosphorus Processing by Trichodesmium Consortia: Quantitative Partitioning, Role of Microbial Coordination, and Impact on Nitrogen Fixation
https://osprey.bco-dmo.org/project/565176
Dissolved Phosphorus Processing by Trichodesmium Consortia: Quantitative Partitioning, Role of Microbial Coordination, and Impact on Nitrogen Fixation
<p><em>Description from NSF award abstract:</em><br />
Colonies of the cyanbacterium <em>Trichodesmium</em> are responsible for a large fraction of N2 fixation in nutrient-poor, open-ocean ecosystems, ultimately fueling primary production in both <em>Trichodesmium</em> and in the broader planktonic community. However, in some parts of the ocean, the scarcity of dissolved phosphorus limits rates of <em>Trichodesmium </em>N2 fixation. <em>Trichodesmium</em> colonies employ an arsenal of strategies to mitigate the effects of phosphorus limitation, and the consortia of epibiotic bacteria in the colonies may play a significant role in phosphorus acquisition.</p>
<p>In this study, researchers from Woods Hole Oceanographic Institution and Columbia University will use metagenomic and metatranscriptomic sequencing to investigate how phosphorus metabolism is coordinated in <em>Trichodesmium </em>consortia, and to discern the role of quorum sensing in phosphorus acquisition and partitioning. Results from this study are expected to expand understanding of <em>Trichodesmium</em> from a monospecific colony whose primary function is fixing CO2 and N2 toward a unique planktonic consortium with a diverse, complex, and highly coordinated overall metabolism that exerts profound control over the cycling of inorganic and organic nutrients in the oligotrophic upper ocean.</p>
P Processing by Tricho
largerWorkCitation
project
eng; USA
oceans
Western Tropical North Atlantic
-64.9907
-52.1795
7.4618
27.8657
2014-05-10
2014-05-27
Western Tropical North Atlantic
0
BCO-DMO catalogue of parameters from Water column data from R/V Atlantic Explorer cruise AE1409 in the Western Tropical North Atlantic from May 2014 (P Processing by Tricho project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/709523.rdf
Name: Date
Units: YYYYMMDD
Description: <p>Sampling date formatted as YYYYMMDD.</p>
http://lod.bco-dmo.org/id/dataset-parameter/709524.rdf
Name: Station
Units: unitless
Description: <p>Numeric identifier for the station where the data was collected.</p>
http://lod.bco-dmo.org/id/dataset-parameter/709525.rdf
Name: Depth
Units: meters
Description: <p>Depth at which the sample was collected.</p>
http://lod.bco-dmo.org/id/dataset-parameter/709526.rdf
Name: Sample
Units: unitless
Description: <p>Numeric identifier of the sample.</p>
http://lod.bco-dmo.org/id/dataset-parameter/709527.rdf
Name: SRP
Units: micromole per liter (umol/L)
Description: <p>Soluble reactive phosphorous (SRP).</p>
http://lod.bco-dmo.org/id/dataset-parameter/709528.rdf
Name: MAGIC_SRP
Units: micromole per liter (umol/L)
Description: <p>soluble reactive phosphorus measured by magnesium-induced co-precipitation</p>
http://lod.bco-dmo.org/id/dataset-parameter/709529.rdf
Name: TDP
Units: micromole per liter (umol/L)
Description: <p>Total dissolved phosphorus.</p>
http://lod.bco-dmo.org/id/dataset-parameter/709530.rdf
Name: DOP_calc
Units: micromole per liter (umol/L)
Description: <p>Dissolved organic phosphorus.</p>
http://lod.bco-dmo.org/id/dataset-parameter/709531.rdf
Name: Lat
Units: Decimal Degrees
Description: <p>Latitude of sampling. Positive indicate North.</p>
http://lod.bco-dmo.org/id/dataset-parameter/709532.rdf
Name: Long
Units: Decimal Degrees
Description: <p>Longitude of sampling. Negative indicate West.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
6153
https://darchive.mblwhoilibrary.org/bitstream/1912/27170/1/dataset-709476_watercolumnae1409__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.709476.1
download
onLine
dataset
<p>MAGIC Soluble reactive phosphorus (SRP) concentrations.&nbsp; SRP (i.e. phosphate) was determined in seawaters samples and incubations was quantified using MAGnesium Induced Coprecipitation (MAGIC) as described by Karl and Tien (1992).</p>
<p>Total dissolved phosphorus (TDP) concentrations – TDP was determined on 0.2 um filtrates of surface water (~5 m depth) samples collected via CTD into acid-clean polycarbonate bottles. Samples were processed at the SOEST Laboratory for Analytical Biogeochemistry at the University of Hawaii, according to facility protocols.</p>
<p>Alkaline phosphatase rates - Alkaline phosphatase activity samples were obtained by placing 2-5 cleaned <em>Trichodesmium </em>colonies on 5 um PC filters, gently vacuum filtering away excess liquid, then storing in 47 mm plastic petri dishes at -20 C until analysis. Samples were processed as previously described by Dyhrman and Ruttenberg (2006) using 6,8-difluoro-4-methylumbeliferyl phosphate (DiMufP) on a Synergy H1 Hybrid plate reader using the Gen5 software package (BioTek, Winooski, VT)</p>
Specified by the Principal Investigator(s)
<p>BCO-DMO Processing:<br />
Added conventional header with dataset name, PI name, version date.<br />
Modified parameter names to conform with BCO-DMO naming conventions.<br />
Re-formatted date from m/dd/yyyy to yyyymmdd.<br />
Replaced spaces and / with underscores.<br />
Added coordinate information to the data from the station list provided by the PI.&nbsp;</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Niskin bottles
Niskin bottles
PI Supplied Instrument Name: Niskin bottles PI Supplied Instrument Description:Water samples for whole community analyses were collected from Niskin bottles deployed on a rosette with a CTD. Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
AA3 Nutrient Autoanalyzer
AA3 Nutrient Autoanalyzer
PI Supplied Instrument Name: AA3 Nutrient Autoanalyzer PI Supplied Instrument Description:Samples were processed at the SOEST Laboratory for Analytical Biogeochemistry at the University of Hawaii and analyzed for phosphate via the AA3 autoanalyzer.
More information about the Phosphorous measurements can be found at the SOEST Laboratory Webiste. Instrument Name: Nutrient Autoanalyzer Instrument Short Name:Nutrient Autoanalyzer Instrument Description: Nutrient Autoanalyzer is a generic term used when specific type, make and model were not specified. In general, a Nutrient Autoanalyzer is an automated flow-thru system for doing nutrient analysis (nitrate, ammonium, orthophosphate, and silicate) on seawater samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/
Synergy H1 Hybrid plate reader
Synergy H1 Hybrid plate reader
PI Supplied Instrument Name: Synergy H1 Hybrid plate reader PI Supplied Instrument Description:Samples were processed as previously described by Dyhrman and Ruttenberg (2006) using 6,8-difluoro-4-methylumbeliferyl phosphate (DiMufP) on a Synergy H1 Hybrid plate reader using the Gen5 software package (BioTek, Winooski, VT).
Reference:
Dyhrman, S.T., Ruttenberg, K.C., 2006. Presence and regulation of alkaline phosphatase activity in eukaryotic phytoplankton from the coastal ocean: Implications for dissolved organic phosphorus remineralization,. Limnol. Oceanogr. 51, 1381-1390. Instrument Name: plate reader Instrument Short Name: Instrument Description: Plate readers (also known as microplate readers) are laboratory instruments designed to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. Sample reactions can be assayed in 6-1536 well format microtiter plates. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 uL per well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µL per well. Common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization. From: http://en.wikipedia.org/wiki/Plate_reader, 2014-09-0-23.
temperature logger (Onset)
temperature logger (Onset)
PI Supplied Instrument Name: temperature logger (Onset) PI Supplied Instrument Description:Temperature in the incubators was occasionally monitored with a waterproof temperature logger (Onset). Instrument Name: Water Temperature Sensor Instrument Short Name:Water Temp Sensor Instrument Description: General term for an instrument that measures the temperature of the water with which it is in contact (thermometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/134/
Cruise: AE1409
AE1409
R/V Atlantic Explorer
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantic Explorer
vessel
AE1409
Benjamin A.S. Van Mooy
Woods Hole Oceanographic Institution
R/V Atlantic Explorer
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantic Explorer
vessel