http://lod.bco-dmo.org/id/dataset/710183
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2017-07-28
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Detection limits for the results of HPLC derived photopigment concentrations for bioassays done in the North Inlet Estuary - Georgetown, South Carolina during 2014 (Photomixotrophy project)
2017-07-28
publication
2017-07-28
revision
BCO-DMO Linked Data URI
2017-07-28
creation
http://lod.bco-dmo.org/id/dataset/710183
James L. Pinckney
University of South Carolina at Columbia
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Pinckney, J. L. (2017) Detection limits for the results of HPLC derived photopigment concentrations for bioassays done in the North Inlet Estuary - Georgetown, South Carolina during 2014 (Photomixotrophy project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version Final) Version Date 2017-07-28 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/710183 [access date]
Detection limits for the results of HPLC – derived photopigment concentrations for bioassays. Dataset Description: <p>Detection limits of the results of <a href="https://www.bco-dmo.org/dataset/710144" target="_blank">HPLC – derived photopigment concentrations for bioassays.</a></p> Methods and Sampling: <p>These are the detection limits for this dataset:&nbsp;<u><a href="https://www.bco-dmo.org/dataset/710144" style="margin: 0px; padding: 0px; border: 0px; font-variant-numeric: inherit; font-stretch: inherit; font-size: 13.68px; line-height: inherit; font-family: Helvetica, Arial, sans-serif; vertical-align: baseline; text-decoration-line: none; color: rgb(0, 102, 153);" target="_blank">HPLC Photopigment Data for Bioassays</a></u></p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1260134 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1260134
completed
James L. Pinckney
University of South Carolina at Columbia
803-546-0768
Baruch Institute
Columbia
SC
29208
USA
pinckney@sc.edu
pointOfContact
asNeeded
Dataset Version: Final
Unknown
Year
Month
Sample_Number
Chl_LOD
Chl_LOQ
Perid_LOD
Perid_LOQ
ButFuc19_LOD
ButFuc19_LOQ
Fuco_LOD
Fuco_LOQ
HexFuc19_LOD
HexFuc19_LOQ
Neo_LOD
Neo_LOQ
Viola_LOD
Viola_LOQ
Diad_LOD
Diad_LOQ
Allox_LOD
Allox_LOQ
Diat_LOD
Diat_LOQ
Lutein_LOD
Lutein_LOQ
Zeax_LOD
Zeax_LOQ
Gyro_LOD
Gyro_LOQ
Chl_b_LOD
Chl_b_LOQ
Chl_a_LOD
Chl_a_LOQ
Temperature-controlled autosampler (Shimadzu SIL10-A vp) with a 500 µl injection loop
Photodiode array detector (PDA, Shimadzu SPD-M10A vp; 200 to 800 nm range)
Flow Scintillation Counter (Packard Radiomatic 525a, 500 ul counting cell)
Binary Gradient Pump (Shimadzu dual LC10-AT vp and Controller SCL-10A vp)
theme
None, User defined
year
No BCO-DMO term
chl_c1_c2
peridinin
19-prime-butanoyloxyfucoxanthin
fucoxanthin
19-prime-hexanoyloxyfucoxanthin
neoxanthin
violaxanthin
diatoxanthin
alloxanthin
lutein
pigment concentration
Gyroxanthin-Diester
chlorophyll b
chlorophyll a
featureType
BCO-DMO Standard Parameters
High-Performance Liquid Chromatograph
High-Performance Liquid Chromatograph
Liquid Scintillation Counter
Pump
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Assimilation rates of dissolved organic carbon by photomixotrophic estuarine phytoplankton
https://sites.google.com/site/jaypinckney/
Assimilation rates of dissolved organic carbon by photomixotrophic estuarine phytoplankton
<p>Phytoplankton, traditionally viewed as primary producers at the base of aquatic food webs, provide an energy source for higher trophic levels. However, some phytoplankton species function as both primary producers and heterotrophic secondary consumers. Phytoplankton that are photosynthetically competent but also take up and assimilate organic compounds are classified as facultative mixotrophs or, more simply, photomixotrophs. Unfortunately, we currently have few estimates of the proportion of the phytoplankton community that function as photomixotrophs, their rate of secondary production, or their temporal variation in abundance. Current paradigms about trophodynamics in marine systems do not consider this potentially important alternative pathway for energy flow for phytoplankton. The implication is that we may be missing a significant, fundamental process that affects carbon cycling and trophodynamics in estuarine systems. Furthermore, changes in the DOC composition due to anthropogenic alterations may result in changes in phytoplankton community structure and possibly promote the proliferation of harmful algal bloom species. In terms of ecosystem function, even moderate rates of photomixotrophy could potentially alter our current understanding of phytoplankton productivity, overall C turnover, competitive interactions, and energy transfer in estuarine environments. This project will use a novel approach to provide quantitative measures of the in situ rates and magnitudes of facultative heterotrophy in natural, estuarine phytoplankton communities over seasonal time scales in a representative estuarine ecosystem. The project will utilize a unique 14C radiolabeling technique to quantify the in situ assimilation rates of DOC by estuarine photomixotrophs and estimate the amount of DOC converted to phytoplankton biomass by photomixotrophy over seasonal time scales. This information will provide new insights into carbon dynamics in estuaries, the contribution of DOC to estuarine food webs, and the importance of photomixotrophy in determining the structural and functional characteristics of estuarine phytoplankton communities.</p>
Photomixotrophy
largerWorkCitation
project
eng; USA
oceans
-79.318359
-79.071852
33.091684
33.452783
2017-07-28
North Inlet, SC: 33 19.5 N, 79 11W
0
BCO-DMO catalogue of parameters from Detection limits for the results of HPLC derived photopigment concentrations for bioassays done in the North Inlet Estuary - Georgetown, South Carolina during 2014 (Photomixotrophy project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/710193.rdf
Name: Year
Units: unitless
Description: <p>Year sample was taken; YYYY</p>
http://lod.bco-dmo.org/id/dataset-parameter/710194.rdf
Name: Month
Units: unitless
Description: <p>Month sample was taken</p>
http://lod.bco-dmo.org/id/dataset-parameter/710195.rdf
Name: Sample_Number
Units: unitless
Description: <p>Sample number</p>
http://lod.bco-dmo.org/id/dataset-parameter/710196.rdf
Name: Chl_LOD
Units: micrograms per liter
Description: <p>Chlorophyll c1+c2 effective limit of detection</p>
http://lod.bco-dmo.org/id/dataset-parameter/710197.rdf
Name: Chl_LOQ
Units: micrograms per liter
Description: <p>Chlorophyll c1+c2 effective limit of quantification</p>
http://lod.bco-dmo.org/id/dataset-parameter/710198.rdf
Name: Perid_LOD
Units: micrograms per liter
Description: <p>Peridinin effective limit of detection</p>
http://lod.bco-dmo.org/id/dataset-parameter/710199.rdf
Name: Perid_LOQ
Units: micrograms per liter
Description: <p>Peridinin effective limit of quanitification</p>
http://lod.bco-dmo.org/id/dataset-parameter/710200.rdf
Name: ButFuc19_LOD
Units: micrograms per liter
Description: <p>19' Butanoyloxyfucoxanthin effective limit of detection</p>
http://lod.bco-dmo.org/id/dataset-parameter/710201.rdf
Name: ButFuc19_LOQ
Units: micrograms per liter
Description: <p>19' Butanoyloxyfucoxanthin effective limit of quantification</p>
http://lod.bco-dmo.org/id/dataset-parameter/710202.rdf
Name: Fuco_LOD
Units: micrograms per liter
Description: <p>Fucoxanthin effective limit of detection</p>
http://lod.bco-dmo.org/id/dataset-parameter/710203.rdf
Name: Fuco_LOQ
Units: micrograms per liter
Description: <p>Fucoxanthin effective limit of quantification</p>
http://lod.bco-dmo.org/id/dataset-parameter/710204.rdf
Name: HexFuc19_LOD
Units: micrograms per liter
Description: <p>19' Hexanoyloxyfucoxathin effective limit of detection</p>
http://lod.bco-dmo.org/id/dataset-parameter/710205.rdf
Name: HexFuc19_LOQ
Units: micrograms per liter
Description: <p>19' Hexanoyloxyfucoxathin effective limit of quantification</p>
http://lod.bco-dmo.org/id/dataset-parameter/710206.rdf
Name: Neo_LOD
Units: micrograms per liter
Description: <p>Neoxanthin effective limit of detection</p>
http://lod.bco-dmo.org/id/dataset-parameter/710207.rdf
Name: Neo_LOQ
Units: micrograms per liter
Description: <p>Neoxanthin effective limit of quantification</p>
http://lod.bco-dmo.org/id/dataset-parameter/710208.rdf
Name: Viola_LOD
Units: micrograms per liter
Description: <p>Violaxanthin effective limit of detection</p>
http://lod.bco-dmo.org/id/dataset-parameter/710209.rdf
Name: Viola_LOQ
Units: micrograms per liter
Description: <p>Violaxanthin effective limit of quantification</p>
http://lod.bco-dmo.org/id/dataset-parameter/710210.rdf
Name: Diad_LOD
Units: micrograms per liter
Description: <p>Diatoxanthin effective limit of detection</p>
http://lod.bco-dmo.org/id/dataset-parameter/710211.rdf
Name: Diad_LOQ
Units: micrograms per liter
Description: <p>Diatoxanthin effective limit of quantification</p>
http://lod.bco-dmo.org/id/dataset-parameter/710212.rdf
Name: Allox_LOD
Units: micrograms per liter
Description: <p>Alloxanthin effective limit of detection</p>
http://lod.bco-dmo.org/id/dataset-parameter/710213.rdf
Name: Allox_LOQ
Units: micrograms per liter
Description: <p>Alloxanthin effective limit of quantification</p>
http://lod.bco-dmo.org/id/dataset-parameter/710214.rdf
Name: Diat_LOD
Units: micrograms per liter
Description: <p>Diatoxanthin effective limit of detection</p>
http://lod.bco-dmo.org/id/dataset-parameter/710215.rdf
Name: Diat_LOQ
Units: micrograms per liter
Description: <p>Diatoxanthin effective limit of quantification</p>
http://lod.bco-dmo.org/id/dataset-parameter/710216.rdf
Name: Lutein_LOD
Units: micrograms per liter
Description: <p>Lutein effective limit of detection</p>
http://lod.bco-dmo.org/id/dataset-parameter/710217.rdf
Name: Lutein_LOQ
Units: micrograms per liter
Description: <p>Lutein effective limit of quantification</p>
http://lod.bco-dmo.org/id/dataset-parameter/710218.rdf
Name: Zeax_LOD
Units: micrograms per liter
Description: <p>Zeaxanthin effective limit of detection</p>
http://lod.bco-dmo.org/id/dataset-parameter/710219.rdf
Name: Zeax_LOQ
Units: micrograms per liter
Description: <p>Zeaxanthin effective limit of quantification</p>
http://lod.bco-dmo.org/id/dataset-parameter/710220.rdf
Name: Gyro_LOD
Units: micrograms per liter
Description: <p>Gyroxanthin effective limit of detection</p>
http://lod.bco-dmo.org/id/dataset-parameter/710221.rdf
Name: Gyro_LOQ
Units: micrograms per liter
Description: <p>Gyroxanthin effective limit of quantification</p>
http://lod.bco-dmo.org/id/dataset-parameter/710222.rdf
Name: Chl_b_LOD
Units: micrograms per liter
Description: <p>Chlorophyll b effective limit of detection</p>
http://lod.bco-dmo.org/id/dataset-parameter/710223.rdf
Name: Chl_b_LOQ
Units: micrograms per liter
Description: <p>Chlorophyll b effective limit of quantification</p>
http://lod.bco-dmo.org/id/dataset-parameter/710224.rdf
Name: Chl_a_LOD
Units: micrograms per liter
Description: <p>Chlorophyll a effective limit of detection</p>
http://lod.bco-dmo.org/id/dataset-parameter/710225.rdf
Name: Chl_a_LOQ
Units: micrograms per liter
Description: <p>Chlorophyll a effective limit of quantification</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
23909
https://datadocs.bco-dmo.org/file/4WW2jRXfzDKp30/detection_limits.csv
detection_limits.csv
Primary data file for dataset ID 710183
download
https://osprey.bco-dmo.org/dataset/710183/data/download
download
onLine
dataset
<p>These are the detection limits for this dataset:&nbsp;<u><a href="https://www.bco-dmo.org/dataset/710144" style="margin: 0px; padding: 0px; border: 0px; font-variant-numeric: inherit; font-stretch: inherit; font-size: 13.68px; line-height: inherit; font-family: Helvetica, Arial, sans-serif; vertical-align: baseline; text-decoration-line: none; color: rgb(0, 102, 153);" target="_blank">HPLC Photopigment Data for Bioassays</a></u></p>
Specified by the Principal Investigator(s)
<p>Methodology from <a href="https://www.researchgate.net/profile/James_Pinckney/publication/265999187_The_USC_HPLC_Method_-Technical_Description/links/55266d0d0cf2628d5afdf397/The-USC-HPLC-Method-Technical-Description.pdf" target="_blank">Pickney, J.L.: The USC HPLC Method - Technical Description.pdf</a></p>
<p><strong>BCO-DMO Data Processing Notes:</strong></p>
<p>-Reformatted column names to comply with BCO-DMO standards.<br />
-Data were originally separated by month (September and November) into two spreadsheets. Data were combined into one file and the columns "month" and "year" were added.<br />
-Colors and headers were removed.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Temperature-controlled autosampler (Shimadzu SIL10-A vp) with a 500 µl injection loop
Temperature-controlled autosampler (Shimadzu SIL10-A vp) with a 500 µl injection loop
PI Supplied Instrument Name: Temperature-controlled autosampler (Shimadzu SIL10-A vp) with a 500 µl injection loop PI Supplied Instrument Description:Used in HPLC Analysis Instrument Name: High-Performance Liquid Chromatograph Instrument Short Name:HPLC Instrument Description: A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB11/
Photodiode array detector (PDA, Shimadzu SPD-M10A vp; 200 to 800 nm range)
Photodiode array detector (PDA, Shimadzu SPD-M10A vp; 200 to 800 nm range)
PI Supplied Instrument Name: Photodiode array detector (PDA, Shimadzu SPD-M10A vp; 200 to 800 nm range) PI Supplied Instrument Description:Used in HPLC analysis Instrument Name: High-Performance Liquid Chromatograph Instrument Short Name:HPLC Instrument Description: A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB11/
Flow Scintillation Counter (Packard Radiomatic 525a, 500 ul counting cell)
Flow Scintillation Counter (Packard Radiomatic 525a, 500 ul counting cell)
PI Supplied Instrument Name: Flow Scintillation Counter (Packard Radiomatic 525a, 500 ul counting cell) PI Supplied Instrument Description:Used to measure chl a Instrument Name: Liquid Scintillation Counter Instrument Short Name:LSC Instrument Description: Liquid scintillation counting is an analytical technique which is defined by the incorporation of the radiolabeled analyte into uniform distribution with a liquid chemical medium capable of converting the kinetic energy of nuclear emissions into light energy. Although the liquid scintillation counter is a sophisticated laboratory counting system used the quantify the activity of particulate emitting (ß and a) radioactive samples, it can also detect the auger electrons emitted from 51Cr and 125I samples.
Liquid scintillation counters are instruments assaying alpha and beta radiation by quantitative detection of visible light produced by the passage of rays or particles through a suitable scintillant incorporated into the sample. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB21/
Binary Gradient Pump (Shimadzu dual LC10-AT vp and Controller SCL-10A vp)
Binary Gradient Pump (Shimadzu dual LC10-AT vp and Controller SCL-10A vp)
PI Supplied Instrument Name: Binary Gradient Pump (Shimadzu dual LC10-AT vp and Controller SCL-10A vp) PI Supplied Instrument Description:Used in HPLC analysis Instrument Name: Pump Instrument Short Name: Instrument Description: A pump is a device that moves fluids (liquids or gases), or sometimes slurries, by mechanical action. Pumps can be classified into three major groups according to the method they use to move the fluid: direct lift, displacement, and gravity pumps