http://lod.bco-dmo.org/id/dataset/712027
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2017-08-03
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Chemical data associated with field collections from the Gulf of Maine, Nauset Marsh Estuary System, and Long Island Sound (Alexandrium isotopes project)
2017-08-03
publication
2017-08-03
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-03-20
publication
https://doi.org/10.1575/1912/bco-dmo.712027.1
Dr Juliette L. Smith
Virginia Institute of Marine Science
principalInvestigator
Donald M. Anderson
Woods Hole Oceanographic Institution
principalInvestigator
Dr Deana L. Erdner
University of Texas - Marine Science Institute
principalInvestigator
Dr James W. McClelland
University of Texas - Marine Science Institute
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Smith, J., Anderson, D., Erdner, D., McClelland, J. (2017) Chemical data associated with field collections from the Gulf of Maine, Nauset Marsh Estuary System, and Long Island Sound (Alexandrium isotopes project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2017-08-03 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.712027.1 [access date]
Chemical data associated with field collections. Dataset Description: <p>Chemical data associated with field collections.</p> Methods and Sampling: <p><strong>Stable Isotope Methods for d15N-NO3-, d18O-NO3-, d15N-POM, and d15N-NH4+: </strong>All filters, glassware, and glass vials used for isotope analysis were pre-combusted for 4 hours at 450C and plastic bottles were acid washed in a 10% HCl bath overnight, to eliminate any crossover N. In preparation for stable isotope analysis, raw seawater was filtered onto a glass fiber filter (25-mm diameter, Whatman GF/D, Cat #: 1823010) to separate particulate organic matter (POM) and dissolved fractions (NO3- and NH4+). Filters were transferred into 20-ml glass vials (Fisher, catalog #05-719-117) and frozen at -20C until isotope analysis of POM (15NPOM). Filtrate, 200 ml, for isotope analysis of dissolved NH4+ (15NNH4+) was processed using a modified version of the NH4+ diffusion method of Holmes (1998), whereby polypropylene membrane filters (25 mm, lot #: 151579, Sterlitech) replaced Teflon filters (Hannon and Bohlke 2008). Isotope analysis of NH4+ and POM was performed on a Finnigan-MAT DeltaPlus Isotope Ratio Monitoring Mass Spectrometer coupled with a Carlo Erba NC 2500 Elemental Analyzer (Model 1108) (Organic Mass Spectrometry Facility, WHOI). Depending on the expected amount of N per sample, the instrument was configured for the typical range of detection, 0.5 to 5 moles N, or modified at the EA combustion furnace to reach a lower detection limit, 0.15 umoles N (Houghton et al. 2000, Holtvoeth et al. 2005, 2006, York et al. 2007). The precision of 15N measurements on this instrument was 0.17‰. Filtrate, 20 ml for N and O stable isotope analysis of dissolved nitrate (15NNO3-) was transferred into duplicate 30-ml HDPE bottles and analyzed by bacteria denitrification assay using ThermoFinnigan GasBench + PreCon trace gas concentration system interfaced to a ThermoScientific Delta V Plus isotope-ratio mass spectrometer (Bremen, Germany) (UC Davis Stable Isotope Facility).</p>
<p><strong>Methods for nutrient quantification:</strong> Dissolved nutrient samples were filtered through a glass fiber filter (25-mm diameter, Whatman GF/F, CAT No. 1825-025). The filtrate was collected in acid-washed 20-ml scintillation vials for nutrient analysis and then frozen at -20 deg C until analysis. A SEAL AA3 four-channel segmented flow analyzer (Nutrient Chemical Facility, Woods Hole Oceanographic Institution, WHOI) was used to quantify NH4+, NO3-/NO2-, Silicate, and PO43- in the GF/F-filtered medium using standard methods.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1505604 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1505604
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1232835 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1232835
completed
Dr Juliette L. Smith
Virginia Institute of Marine Science
804-684-7289
PO Box 1346
Gloucester Point
VA
23062
USA
jlsmith@vims.edu
pointOfContact
Donald M. Anderson
Woods Hole Oceanographic Institution
508-289-2351
Woods Hole Oceanographic Institution Department of Biology - MS #32
Woods Hole
MA
02543
USA
danderson@whoi.edu
pointOfContact
Dr Deana L. Erdner
University of Texas - Marine Science Institute
361-749-6719
750 Channel View Dr.
Port Aransas
TX
78373
USA
derdner@utexas.edu
pointOfContact
Dr James W. McClelland
University of Texas - Marine Science Institute
361-749-6756
750 Channel View Dr.
Port Aransas
TX
78373
USA
jimm@utexas.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Date
Sample_ID
Description
Depth
d15N_NO3
d18O_NO3
d15N_NH4
d15N_POM
NO3
NH4
PO4
Silicate
SEAL AA3 four-channel segmented flow analyzer
Carlo Erba NC 2500 Elemental Analyzer (Model 1108)
ThermoFinnigan GasBench + PreCon trace gas concentration system
Finnigan-MAT DeltaPlus Isotope Ratio Monitoring Mass Spectrometer
ThermoScientific Delta V Plus isotope-ratio mass spectrometer
theme
None, User defined
date
sample identification
brief description
depth
d15N
d18O_NO3
Nitrate
Ammonium
reactive phosphorus (PO4)
Silicate (reactive silica)
featureType
BCO-DMO Standard Parameters
Continuous Flow Analyzer
Elemental Analyzer
Gas Analyzer
Isotope-ratio Mass Spectrometer
Isotope-ratio Mass Spectrometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Identification of nitrogen sources for toxic Alexandrium blooms using a novel species-specific tracer, d15N-saxitoxin
http://www.vims.edu/research/departments/eaah/programs/aquatic_toxinology/research/isotope_project.php
Collaborative Research: Identification of nitrogen sources for toxic Alexandrium blooms using a novel species-specific tracer, d15N-saxitoxin
<p><em>NSF award abstract:</em><br />
The US and other countries throughout the world are affected by harmful algal blooms (HABs) that negatively impact human health, marine ecosystems, fisheries resources, and local economies. Anthropogenic nutrient loadings have been linked to expanding HAB incidence, but the relationship is site- and organism-specific, and is still poorly understood. The main challenge in this regard is to determine the relative importance of natural versus anthropogenic nutrient sources in the development of an individual HAB species. Given the diverse nature of the planktonic assemblage in which HABs occur, and the lack of appropriate measurement techniques, this is exceedingly difficult to accomplish.</p>
<p>In this project, research teams at the Woods Hole Oceanographic Institution and University of Texas at Austin will take a novel approach to this challenge: They use use the nitrogen isotopic signature (del15N) of a species-specific HAB toxin to identify the nitrogen source and chemical form that promotes cell growth and toxin production. The bloom-forming dinoflagellate Alexandrium fundyense and its class of bioactive compounds, saxitoxins (STXs), are an ideal model system as STXs are nitrogen-rich and are typically only produced by a single species in mixed plankton assemblages. The guiding overall hypothesis is that the isotopic signature of a HAB-specific toxin can be used to discriminate between anthropogenic and natural sources of N and provide more details than bulk material del15N on the source, chemical form, and processing of N that lead to blooms of a particular toxic species. This hypothesis is based on the principle that human and animal waste in groundwater and sewage become 15N-enriched and inorganic fertilizers 15N-depleted, relative to natural sources of N in catchment waters. While the use of the isotopic ratio del15N of bulk biomass to identify nitrogen sources to coastal waters is a widely accepted practice, this use of a toxin as a species-specific tracer or marker is new and will provide details on the explicit source, chemical form, and processing of nitrogen that results in blooms of a particular HAB species.</p>
<p>Broader Impacts: This project addresses fundamental issues underlying the most widespread of all HAB poisoning syndromes, paralytic shellfish poisoning (PSP), a major form of shellfish poisoning that affects countries throughout the world. Project results can also assist in policy decisions about pollution control and other bloom mitigation strategies, and can be applied to a range of HAB species - those that produce saxitoxins, as well as those that produce other toxins that are nitrogen rich. Project results will be broadly disseminated through scientific papers, presentations at workshops, domestic and international conferences, and departmental seminars, and discussions with the media.</p>
Alexandrium-isotopes
largerWorkCitation
project
eng; USA
oceans
-73.772282
-67.672347
40.816797
44.235499
2014-03-12
2015-06-18
Gulf of Maine, Nauset Marsh Estuary System (Cape Cod Seashore), Northport Huntington Bay Complex (Long Island Sound)
0
BCO-DMO catalogue of parameters from Chemical data associated with field collections from the Gulf of Maine, Nauset Marsh Estuary System, and Long Island Sound (Alexandrium isotopes project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/712044.rdf
Name: Date
Units: unitless
Description: <p>Date sample was taken; YYYY/MM/DD</p>
http://lod.bco-dmo.org/id/dataset-parameter/712045.rdf
Name: Sample_ID
Units: unitless
Description: <p>Sample ID; short name/abbreviation</p>
http://lod.bco-dmo.org/id/dataset-parameter/712046.rdf
Name: Description
Units: unitless
Description: <p>Full description of site and station where sample was taken</p>
http://lod.bco-dmo.org/id/dataset-parameter/712047.rdf
Name: Depth
Units: meters
Description: <p>Depth that sample was taken</p>
http://lod.bco-dmo.org/id/dataset-parameter/712048.rdf
Name: d15N_NO3
Units: per mil (0/00)
Description: <p>Stable isotope value; d15N - NO3</p>
http://lod.bco-dmo.org/id/dataset-parameter/712049.rdf
Name: d18O_NO3
Units: per mil (0/00)
Description: <p>Stable isotope value; d18O - NO3</p>
http://lod.bco-dmo.org/id/dataset-parameter/712050.rdf
Name: d15N_NH4
Units: per mil (0/00)
Description: <p>Stable isotope value; d15N - NH4</p>
http://lod.bco-dmo.org/id/dataset-parameter/712051.rdf
Name: d15N_POM
Units: per mil (0/00)
Description: <p>Stable isotope value; d15N - POM</p>
http://lod.bco-dmo.org/id/dataset-parameter/712052.rdf
Name: NO3
Units: micromoles per liter
Description: <p>Dissolved nutrient concentration; NO3-</p>
http://lod.bco-dmo.org/id/dataset-parameter/712053.rdf
Name: NH4
Units: micromoles per liter
Description: <p>Dissolved nutrient concentration; NH4</p>
http://lod.bco-dmo.org/id/dataset-parameter/712054.rdf
Name: PO4
Units: micromoles per liter
Description: <p>Dissolved nutrient concentration; PO4</p>
http://lod.bco-dmo.org/id/dataset-parameter/712055.rdf
Name: Silicate
Units: micromoles per liter
Description: <p>Dissolved nutrient concentration; Silicate</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
4849
https://darchive.mblwhoilibrary.org/bitstream/1912/23893/1/dataset-712027_chemical-data__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.712027.1
download
onLine
dataset
<p><strong>Stable Isotope Methods for d15N-NO3-, d18O-NO3-, d15N-POM, and d15N-NH4+: </strong>All filters, glassware, and glass vials used for isotope analysis were pre-combusted for 4 hours at 450C and plastic bottles were acid washed in a 10% HCl bath overnight, to eliminate any crossover N. In preparation for stable isotope analysis, raw seawater was filtered onto a glass fiber filter (25-mm diameter, Whatman GF/D, Cat #: 1823010) to separate particulate organic matter (POM) and dissolved fractions (NO3- and NH4+). Filters were transferred into 20-ml glass vials (Fisher, catalog #05-719-117) and frozen at -20C until isotope analysis of POM (15NPOM). Filtrate, 200 ml, for isotope analysis of dissolved NH4+ (15NNH4+) was processed using a modified version of the NH4+ diffusion method of Holmes (1998), whereby polypropylene membrane filters (25 mm, lot #: 151579, Sterlitech) replaced Teflon filters (Hannon and Bohlke 2008). Isotope analysis of NH4+ and POM was performed on a Finnigan-MAT DeltaPlus Isotope Ratio Monitoring Mass Spectrometer coupled with a Carlo Erba NC 2500 Elemental Analyzer (Model 1108) (Organic Mass Spectrometry Facility, WHOI). Depending on the expected amount of N per sample, the instrument was configured for the typical range of detection, 0.5 to 5 moles N, or modified at the EA combustion furnace to reach a lower detection limit, 0.15 umoles N (Houghton et al. 2000, Holtvoeth et al. 2005, 2006, York et al. 2007). The precision of 15N measurements on this instrument was 0.17‰. Filtrate, 20 ml for N and O stable isotope analysis of dissolved nitrate (15NNO3-) was transferred into duplicate 30-ml HDPE bottles and analyzed by bacteria denitrification assay using ThermoFinnigan GasBench + PreCon trace gas concentration system interfaced to a ThermoScientific Delta V Plus isotope-ratio mass spectrometer (Bremen, Germany) (UC Davis Stable Isotope Facility).</p>
<p><strong>Methods for nutrient quantification:</strong> Dissolved nutrient samples were filtered through a glass fiber filter (25-mm diameter, Whatman GF/F, CAT No. 1825-025). The filtrate was collected in acid-washed 20-ml scintillation vials for nutrient analysis and then frozen at -20 deg C until analysis. A SEAL AA3 four-channel segmented flow analyzer (Nutrient Chemical Facility, Woods Hole Oceanographic Institution, WHOI) was used to quantify NH4+, NO3-/NO2-, Silicate, and PO43- in the GF/F-filtered medium using standard methods.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Data Processing Notes:</strong></p>
<p>-Reformatted column names to comply with BCO-DMO standards.<br />
-Removed first header line that contained descriptive metadata. This was added into the appropriate parameter descriptions.<br />
-Dates were reformatted from mm/dd/yy to yyyy/mm/dd.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
SEAL AA3 four-channel segmented flow analyzer
SEAL AA3 four-channel segmented flow analyzer
PI Supplied Instrument Name: SEAL AA3 four-channel segmented flow analyzer PI Supplied Instrument Description:Used to quantify NH4+, NO3-/NO2-, Silicate, and PO43- Instrument Name: Continuous Flow Analyzer Instrument Short Name:CFA Instrument Description: A sample is injected into a flowing carrier solution passing rapidly through small-bore tubing.
Carlo Erba NC 2500 Elemental Analyzer (Model 1108)
Carlo Erba NC 2500 Elemental Analyzer (Model 1108)
PI Supplied Instrument Name: Carlo Erba NC 2500 Elemental Analyzer (Model 1108) PI Supplied Instrument Description:Used to perform isotope analysis of NH4+ and POM Instrument Name: Elemental Analyzer Instrument Short Name: Instrument Description: Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/
ThermoFinnigan GasBench + PreCon trace gas concentration system
ThermoFinnigan GasBench + PreCon trace gas concentration system
PI Supplied Instrument Name: ThermoFinnigan GasBench + PreCon trace gas concentration system PI Supplied Instrument Description:Used to analyze N and O stable isotopes Instrument Name: Gas Analyzer Instrument Short Name:Gas Analyzer Instrument Description: Gas Analyzers - Instruments for determining the qualitative and quantitative composition of gas mixtures.
Finnigan-MAT DeltaPlus Isotope Ratio Monitoring Mass Spectrometer
Finnigan-MAT DeltaPlus Isotope Ratio Monitoring Mass Spectrometer
PI Supplied Instrument Name: Finnigan-MAT DeltaPlus Isotope Ratio Monitoring Mass Spectrometer PI Supplied Instrument Description:Used to perform isotope analysis of NH4+ and POM Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
ThermoScientific Delta V Plus isotope-ratio mass spectrometer
ThermoScientific Delta V Plus isotope-ratio mass spectrometer
PI Supplied Instrument Name: ThermoScientific Delta V Plus isotope-ratio mass spectrometer PI Supplied Instrument Description:Used to analyze N and O stable isotopes Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/