http://lod.bco-dmo.org/id/dataset/712366
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2017-08-08
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Acropora cervicornis photosynthesis and respiration rates under different pH and temperature treatments from experiments at Summerland Key, Florida in September of 2016
2017-08-08
publication
2017-08-08
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-10-18
publication
https://doi.org/10.1575/1912/bco-dmo.712366.1
Erinn M. Muller
Mote Marine Laboratory
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Muller, E. (2017) Acropora cervicornis photosynthesis and respiration rates under different pH and temperature treatments from experiments at Summerland Key, Florida in September of 2016. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2017-08-08 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.712366.1 [access date]
Photosynthesis and Respiration rates of Acropora cervicornis Dataset Description: <p>This dataset contains oxygen change rates under dark and light conditions from 12&nbsp;Acropora cervicornis genotypes previously exposed to different pH and temperature treatments. The coral surface area during photosynthesis and respiration experiments is included.&nbsp; The experiments were conducted in tanks at&nbsp;Summerland Key, Florida (24.6616,-81.4538) between 2016-09-02 and 2016-09-10 with corals from a&nbsp;nursery located near Looe Key Reef&nbsp;(24.5636, -81.2786).</p> Methods and Sampling: <p>Physiological Methods</p>
<p>Photosynthesis, respiration, and calcification measurements were performed on each fragment using 300 mL temperature-controlled respirometry chambers filled with seawater from the treatment aquaria that was continuously stirred with a magnetic stir bar.&nbsp; The chambers were used to assess the rates of respiration (Rd) in the dark and rates of photosynthesis (Pn) and calcification in the light.&nbsp; Light was supplied by a series of blue and red LEDs with adjustable intensity (150 uMol quanta m-2 sec-1).&nbsp;&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1452538 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1452538
completed
Erinn M. Muller
Mote Marine Laboratory
941-388-4441 x 310
1600 Ken Thompson Parkway
Sarasota
FL
34241
USA
emuller@mote.org
pointOfContact
asNeeded
Dataset Version: 1
Unknown
date
O2_rate_dark
O2_rate_light
genotype
tank
temp
pH
area
optodes (Oxy-4, Presense, Germany)
Mettler Toledo SevenGo Pro
YSI Pro 2030
theme
None, User defined
date
oxygen respiration rates from in situ incubation
sample description
tank
water temperature at measurement depth
pH
surface_area
featureType
BCO-DMO Standard Parameters
Optode
pH Sensor
YSI Professional Plus Multi-Parameter Probe
instrument
BCO-DMO Standard Instruments
Muller_Looe_Key_Reef_Acropora
service
Deployment Activity
Looe Key Reef
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
CAREER: Applying phenotypic variability to identify resilient Acropora cervicornis genotypes in the Florida Keys
https://osprey.bco-dmo.org/project/642850
CAREER: Applying phenotypic variability to identify resilient Acropora cervicornis genotypes in the Florida Keys
<p><em>NSF Award Abstract:</em><br />
Caribbean staghorn coral was one of the most common corals within reefs of the Florida Keys several decades ago. Over the last 40 years disease, bleaching, overfishing and habitat degradation caused a 95% reduction of the population. Staghorn coral is now listed as threatened under the U.S. Endangered Species Act of 1973. Within the past few years, millions of dollars have been invested for the purpose of restoring the population of staghorn coral within Florida and the U.S. Virgin Islands. Significant effort has been placed on maintaining and propagating corals of known genotypes within coral nurseries for the purpose of outplanting. However, little is known about the individual genotypes that are currently being outplanted from nurseries onto coral reefs. Are the genotypes being used for outplanting resilient enough to survive the three major stressors affecting the population in the Florida Keys: disease, high water temperatures, and ocean acidification? The research within the present study will be the first step in answering this critically important question. The funded project will additionally develop a research-based afterschool program with K-12 students in the Florida Keys and U.S. Virgin Islands that emphasizes an inquiry-based curriculum, STEM research activities, and peer-to-peer mentoring. The information from the present study will help scientists predict the likelihood of species persistence within the lower Florida Keys under future climate-change and ocean-acidification scenarios. Results of this research will also help guide restoration efforts throughout Florida and the Caribbean, and lead to more informative, science-based restoration activities.</p>
<p><em>Acropora cervicornis</em> dominated shallow-water reefs within the Florida Keys for at least the last half a million years, but the population has recently declined due to multiple stressors. Understanding the current population level of resilience to three major threats - disease outbreaks, high water temperatures, and ocean acidification conditions - is critical for the preservation of this threatened species. Results from the present study will answer the primary research question: will representative genotypes from the lower Florida Keys provide enough phenotypic variation for this threatened species to survive in the future? The present proposal will couple controlled laboratory challenge experiments with field data and modeling applications, and collaborate with local educators to fulfill five objectives: 1) identify <em>A. cervicornis</em> genotypes resistant to disease, 2) identify <em>A. cervicornis</em> genotypes resilient to high water temperature and ocean acidification conditions, 3) quantify how high water temperature and ocean acidification conditions impact disease dynamics on <em>A. cervicornis</em>; 4) determine tradeoffs in life-history traits because of resilience factors; and 5) apply a trait-based model, which will predict genotypic structure of a population under different environmental scenarios.</p>
Resilient Acerv
largerWorkCitation
project
eng; USA
oceans
Looe Key Reef
-81.453789
-81.2786
24.5636
24.661603
2016-09-02
2016-09-10
Florida Keys, Summerland Key, FL 24.563595°, -81.278572°
0
BCO-DMO catalogue of parameters from Acropora cervicornis photosynthesis and respiration rates under different pH and temperature treatments from experiments at Summerland Key, Florida in September of 2016
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/712424.rdf
Name: date
Units: unitless
Description: <p>Day the measurement took place in format yyyy-mm-dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/712425.rdf
Name: O2_rate_dark
Units: micromoles of oxygen per hour (umol/h)
Description: <p>Rate of change (slope) in oxygen levels as coral sat in the dark for 60 minutes</p>
http://lod.bco-dmo.org/id/dataset-parameter/712426.rdf
Name: O2_rate_light
Units: micromoles of oxygen per hour (umol/h)
Description: <p>Rate of change (slope) in oxygen levels as coral sat in light conditions for 60 minutes</p>
http://lod.bco-dmo.org/id/dataset-parameter/712427.rdf
Name: genotype
Units: unitless
Description: <p>Genotype number of the coral animal for each fragment</p>
http://lod.bco-dmo.org/id/dataset-parameter/712428.rdf
Name: tank
Units: unitless
Description: <p>Tank number that held the particular coral fragment</p>
http://lod.bco-dmo.org/id/dataset-parameter/712429.rdf
Name: temp
Units: Celsius
Description: <p>Treatment temperature level</p>
http://lod.bco-dmo.org/id/dataset-parameter/712430.rdf
Name: pH
Units: unitless
Description: <p>Treatment pH level; ambient = 8.1 pH; hCO2 = 7.7 pH</p>
http://lod.bco-dmo.org/id/dataset-parameter/712431.rdf
Name: area
Units: centimeters squared (cm2)
Description: <p>Surface area of the coral</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
11230
https://darchive.mblwhoilibrary.org/bitstream/1912/24703/1/dataset-712366_acropora-cervicornis-photosynthesis-and-respiration-rates__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.712366.1
download
onLine
dataset
<p>Physiological Methods</p>
<p>Photosynthesis, respiration, and calcification measurements were performed on each fragment using 300 mL temperature-controlled respirometry chambers filled with seawater from the treatment aquaria that was continuously stirred with a magnetic stir bar.&nbsp; The chambers were used to assess the rates of respiration (Rd) in the dark and rates of photosynthesis (Pn) and calcification in the light.&nbsp; Light was supplied by a series of blue and red LEDs with adjustable intensity (150 uMol quanta m-2 sec-1).&nbsp;&nbsp;</p>
Specified by the Principal Investigator(s)
<div>Photosynthesis and Respiration - The concentration of dissolved oxygen (O2, umol L-1) was continuously measured inside the chambers using optrodes (Oxy-4, Presense, Germany) to determine Rd (oxygen consumption) and Pn (oxygen production) in umol O2 cm-2 h-1. &nbsp;Rd rates were measured during incubation in complete darkness (60 minutes). &nbsp;Subsequently, light intensity was increased to 100 umol photons m-2 s-1 and Pn rates were measured (60 minutes). &nbsp; Rd and Pn were calculated as: Pn (or Rd) O2 = (sO2 x V)/S where sO2 is the slope of the linear regression line for change in O2 over time (umol L-1 h-1), V is the volume of the chamber (l) and S is the surface area of the fragment (cm2).&nbsp;</div>
<div>&nbsp;</div>
<div><strong>BCO-DMO Data Manager Processing Notes:</strong><br />
* added a conventional header with dataset name, PI name, version date<br />
* modified parameter names to conform with BCO-DMO naming conventions</div>
<div>* renamed dark..umol.O2.h. light..umol.O2.h. to "O2_rate_dark" and "O2_rate_light" due to characer restrictions (no periods).</div>
<div>* rounded O2_rate dark and light to three decimal places<br />
* "NA" and "na" changed to "nd" for "no data"</div>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
optodes (Oxy-4, Presense, Germany)
optodes (Oxy-4, Presense, Germany)
PI Supplied Instrument Name: optodes (Oxy-4, Presense, Germany) Instrument Name: Optode Instrument Short Name: Instrument Description: An optode or optrode is an optical sensor device that optically measures a specific substance usually with the aid of a chemical transducer.
Mettler Toledo SevenGo Pro
Mettler Toledo SevenGo Pro
PI Supplied Instrument Name: Mettler Toledo SevenGo Pro Instrument Name: pH Sensor Instrument Short Name:pH Sensor Instrument Description: An instrument that measures the hydrogen ion activity in solutions.
The overall concentration of hydrogen ions is inversely related to its pH. The pH scale ranges from 0 to 14 and indicates whether acidic (more H+) or basic (less H+).
YSI Pro 2030
YSI Pro 2030
PI Supplied Instrument Name: YSI Pro 2030 PI Supplied Instrument Description:Temperature, salinity, and dissolved oxygen measured with YSI Pro 2030. Instrument Name: YSI Professional Plus Multi-Parameter Probe Instrument Short Name:YSI ProPlus Instrument Description: The YSI Professional Plus handheld multiparameter meter provides for the measurement of a variety of combinations for dissolved oxygen, conductivity, specific conductance, salinity, resistivity, total dissolved solids (TDS), pH, ORP, pH/ORP combination, ammonium (ammonia), nitrate, chloride and temperature. More information from the manufacturer.
Deployment: Muller_Looe_Key_Reef_Acropora
Muller_Looe_Key_Reef_Acropora
Mote Offshore Coral Nursery
island
Muller_Looe_Key_Reef_Acropora
Erinn M. Muller
Mote Marine Laboratory
Mote Offshore Coral Nursery
island