http://lod.bco-dmo.org/id/dataset/712388
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2017-08-08
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Imaging pulse amplitude modulator fluorometer data collected during Acropora cervicornis experiments at Summerland Key, Florida from July to September of 2016
2017-08-08
publication
2017-08-08
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-10-18
publication
https://doi.org/10.1575/1912/bco-dmo.712388.1
Erinn M. Muller
Mote Marine Laboratory
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Muller, E. (2017) Imaging pulse amplitude modulator fluorometer data collected during Acropora cervicornis experiments at Summerland Key, Florida from July to September of 2016. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2017-08-08 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.712388.1 [access date]
Imaging Pulse Amplitude Modulator Fluorometer Data Dataset Description: <p>This dataset contains&nbsp;Imaging Pulse Amplitude Modulator (IPAM) Fluorometer data collected during various Acropora cervicornis treatment experiments. The data include photosynthetic&nbsp;maximum yield (mY), maximum electron transport rate (mETR), the irradiance at which maximum ETR is reached (mPAR), and the initial slope of the ETR curve (alpha). The experiments were conducted in tanks at&nbsp;Summerland Key, Florida (24.6616,-81.4538) between 2016-07-12 and&nbsp;2016-09-09 with corals from a&nbsp;nursery located near Looe Key Reef&nbsp;(24.5636, -81.2786).&nbsp;</p>
<p>The tank numbers listed in this dataset&nbsp;are the same tanks included in the following datasets:</p>
<p><a href="http://https://www.bco-dmo.org/dataset/712367" target="_blank">Acropora cervicornis buoyant weight</a><br />
<a href="https://www.bco-dmo.org/dataset/712377" target="_blank">Acropora cervicornis calcification rates</a><br />
<a href="https://www.bco-dmo.org/dataset/712366" target="_blank">Acropora cervicornis photosynthesis and respiration rates</a></p>
<p><br />
&nbsp;</p> Methods and Sampling: <p>Corals from different treatment scenarios were IPAMed every other week for the duration of the two-month long experiment. All fragments were subjected to a light curve where the initial pulse represents the max Yield after dark acclimation and the subsequent electron transport rate (ETR) values are recorded after the corals were exposed to increasing light intensities over time.&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1452538 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1452538
completed
Erinn M. Muller
Mote Marine Laboratory
941-388-4441 x 310
1600 Ken Thompson Parkway
Sarasota
FL
34241
USA
emuller@mote.org
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Date
Genotype
Tank
mYield
mETR
mPAR
alpha
IPAM MAXI/L Fluorometer with the IMAG-K6 camera
theme
None, User defined
date
sample description
tank
photosynthetic rate
Electron Transport Rate (ETR)
PAR
alpha
featureType
BCO-DMO Standard Parameters
Fluorometer
instrument
BCO-DMO Standard Instruments
Muller_Looe_Key_Reef_Acropora
service
Deployment Activity
Looe Key Reef
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
CAREER: Applying phenotypic variability to identify resilient Acropora cervicornis genotypes in the Florida Keys
https://osprey.bco-dmo.org/project/642850
CAREER: Applying phenotypic variability to identify resilient Acropora cervicornis genotypes in the Florida Keys
<p><em>NSF Award Abstract:</em><br />
Caribbean staghorn coral was one of the most common corals within reefs of the Florida Keys several decades ago. Over the last 40 years disease, bleaching, overfishing and habitat degradation caused a 95% reduction of the population. Staghorn coral is now listed as threatened under the U.S. Endangered Species Act of 1973. Within the past few years, millions of dollars have been invested for the purpose of restoring the population of staghorn coral within Florida and the U.S. Virgin Islands. Significant effort has been placed on maintaining and propagating corals of known genotypes within coral nurseries for the purpose of outplanting. However, little is known about the individual genotypes that are currently being outplanted from nurseries onto coral reefs. Are the genotypes being used for outplanting resilient enough to survive the three major stressors affecting the population in the Florida Keys: disease, high water temperatures, and ocean acidification? The research within the present study will be the first step in answering this critically important question. The funded project will additionally develop a research-based afterschool program with K-12 students in the Florida Keys and U.S. Virgin Islands that emphasizes an inquiry-based curriculum, STEM research activities, and peer-to-peer mentoring. The information from the present study will help scientists predict the likelihood of species persistence within the lower Florida Keys under future climate-change and ocean-acidification scenarios. Results of this research will also help guide restoration efforts throughout Florida and the Caribbean, and lead to more informative, science-based restoration activities.</p>
<p><em>Acropora cervicornis</em> dominated shallow-water reefs within the Florida Keys for at least the last half a million years, but the population has recently declined due to multiple stressors. Understanding the current population level of resilience to three major threats - disease outbreaks, high water temperatures, and ocean acidification conditions - is critical for the preservation of this threatened species. Results from the present study will answer the primary research question: will representative genotypes from the lower Florida Keys provide enough phenotypic variation for this threatened species to survive in the future? The present proposal will couple controlled laboratory challenge experiments with field data and modeling applications, and collaborate with local educators to fulfill five objectives: 1) identify <em>A. cervicornis</em> genotypes resistant to disease, 2) identify <em>A. cervicornis</em> genotypes resilient to high water temperature and ocean acidification conditions, 3) quantify how high water temperature and ocean acidification conditions impact disease dynamics on <em>A. cervicornis</em>; 4) determine tradeoffs in life-history traits because of resilience factors; and 5) apply a trait-based model, which will predict genotypic structure of a population under different environmental scenarios.</p>
Resilient Acerv
largerWorkCitation
project
eng; USA
biota
Looe Key Reef
-81.453789
-81.2786
24.5636
24.661603
2016-07-12
2016-09-09
Florida Keys, Summerland Key, FL 24.563595°, -81.278572°
0
BCO-DMO catalogue of parameters from Imaging pulse amplitude modulator fluorometer data collected during Acropora cervicornis experiments at Summerland Key, Florida from July to September of 2016
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/712439.rdf
Name: Date
Units: unitless
Description: <p>Day the measurement took place in format yyyy-mm-dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/712440.rdf
Name: Genotype
Units: unitless
Description: <p>Genotype number of the coral animal for each fragment</p>
http://lod.bco-dmo.org/id/dataset-parameter/712441.rdf
Name: Tank
Units: unitless
Description: <p>Tank number that held the particular coral fragment</p>
http://lod.bco-dmo.org/id/dataset-parameter/712442.rdf
Name: mYield
Units: unitless but represents the proportion of electrons being used for photosynthesis; potential values range from 0 - 1
Description: <p>Photosynthetic yield after dark acclimation</p>
http://lod.bco-dmo.org/id/dataset-parameter/712443.rdf
Name: mETR
Units: micromoles of electrons per meter per second (umol electrons/m/s)
Description: <p>Maximum level of electron transport rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/712444.rdf
Name: mPAR
Units: micromoles of photons per meter per second (umol photons/m/s)
Description: <p>Level of photosynthetically active radiation (PAR) at maximum electron transfer rate (ETR)</p>
http://lod.bco-dmo.org/id/dataset-parameter/712445.rdf
Name: alpha
Units: micromoles of electrons per meter per second (umol electrons/m/s)
Description: <p>Slope of the electron transport rate (ETR); units represent the change in ETR over increasing values of PAR</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
44079
https://darchive.mblwhoilibrary.org/bitstream/1912/24704/1/dataset-712388_imaging-pulse-amplitude-modulator-fluorometer-data__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.712388.1
download
onLine
dataset
<p>Corals from different treatment scenarios were IPAMed every other week for the duration of the two-month long experiment. All fragments were subjected to a light curve where the initial pulse represents the max Yield after dark acclimation and the subsequent electron transport rate (ETR) values are recorded after the corals were exposed to increasing light intensities over time.&nbsp;</p>
Specified by the Principal Investigator(s)
<p>Data Processing:&nbsp;<br />
We derived four key parameters from the IPAM photophysiology data set: maximum yield (mY), maximum electron transport rate (mETR), the irradiance at which maximum ETR is reached (mPAR), and the initial slope of the ETR curve (alpha). Only mY was transformed, using a skewed and heavy-tailed LambertWxN back-transform to induce normality, though the lower and upper tails still remain heavier and lighter than the normal distribution respectively.&nbsp;<br />
PAM dataset parameters: Maximum electron transport rate (mETR) was estimated using a linear search algorithm that used the slopes between adjacent (PAR, ETR) data points to determine the first local critical point (maximum or plateau). The algorithm first searches for three data points between which the slope changes sign. If no sign change is detected, the algorithm then looks for largest set of two data points between which the slope is less than a user defined threshold (default is 0.1). In the former case, the middle point is chosen as the (mPAR, mETR) coordinate, while in the later case the second endpoint is used. If neither search yields a solution, the threshold is increased by 0.01 and the two linear searches are repeated, until a solution is found. The slope alpha is calculated with a similar algorithm that searches all PAR levels x such that min(PAR) = 55 &lt; x &lt; mPAR for pairs of points between which the ratio of the local slope to the initial slope (slope between (0,0) and first non-zero datapoint) is less than a user defined threshold (default is 0.1). If not solution is found, the threshold is increased by 0.01 and the search is repeated. Once a pair of points i and i+1 have been identified, all data points with PAR levels less than that of point i are used to calculate the slope. &nbsp;If the threshold increases to greater than 0.25, the algorithm terminates by returning the slope of all points with PAR &lt; mPAR. Both algorithms return NA when given zero vectors and are both implemented in R.&nbsp;</p>
<p><strong>BCO-DMO Data Manager Processing Notes:</strong><br />
* added a conventional header with dataset name, PI name, version date<br />
* modified parameter names to conform with BCO-DMO naming conventions<br />
* alpha values rounded to three decimal places</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
IPAM MAXI/L Fluorometer with the IMAG-K6 camera
IPAM MAXI/L Fluorometer with the IMAG-K6 camera
PI Supplied Instrument Name: IPAM MAXI/L Fluorometer with the IMAG-K6 camera Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/
Deployment: Muller_Looe_Key_Reef_Acropora
Muller_Looe_Key_Reef_Acropora
Mote Offshore Coral Nursery
island
Muller_Looe_Key_Reef_Acropora
Erinn M. Muller
Mote Marine Laboratory
Mote Offshore Coral Nursery
island