http://lod.bco-dmo.org/id/dataset/712626
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2017-08-10
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Initial prey abundances for copepod grazing experiments in the Kaneohe Bay, HI, May-June 2013 (MEPS 2017) (EAGER: Copepod nauplii project)
2017-09-01
publication
2017-09-01
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2021-01-20
publication
https://doi.org/10.26008/1912/bco-dmo.712626.1
Erica Goetze
University of Hawaiʻi at Mānoa
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Goetze, E. (2021) Initial prey abundances for copepod grazing experiments in the Kaneohe Bay, HI, May-June 2013 (MEPS 2017) (EAGER: Copepod nauplii project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2017-09-01 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.712626.1 [access date]
Initial prey abundance and biomass: MEPS 2017 Dataset Description: Methods and Sampling: <p>From Jungbluth et al. 2017 – MEPS</p>
<p><strong>Prey size spectra and abundance (Coulter Counter)</strong></p>
<p>Initial and final time-point Coulter Counter (CC) samples were taken for prey particle spectra by gently pouring 20 ml from each incubation bottle through a 35 µm cap filter into a clean beaker, then gently back-washing the filter into the experimental bottle to return any nauplii using a small volume of 0.2 µm filtered seawater. From this subsample, triplicate 2 ml volumes were measured with a Beckman Coulter Multisizer III CC with a 100 µm orifice tube, yielding a spectrum of particle sizes from 2–35 µm ESD, as well as quantitative abundance data. These raw data were further processed in R (R Core Team 2016) to streamline binning of prey size groups, for calculations of clearance and ingestion rates, and for statistical analyses.</p>
<p>Prey ESD was converted to biovolume (BV; µm 3), then to carbon (C; pg C cell–1), using the relationship C = 0.216 × BV0.939, which applies well to taxonomically diverse protists (Menden-Deuer &amp; Lessard 2000). Averages of cell abundance and biomass were binned into 5 prey size groupings (2–5, 5–10, 10–15, 15–20, and 20–35 µm). The binned, averaged data for initial and final time points for each control and treatment bottle were used to calculate carbon ingestion (I; ng C nauplius–1 h–1) and clearance rates (F, ml nauplius–1 h–1) on each prey size group using the equations of Frost (1972).</p>
<p><strong>Photosynthetic eukaryotes (flow cytometry)</strong></p>
<p>Flow cytometry (FCM) samples (1.5 ml) for photosynthetic eukaryote (PEUK) abundance were taken from the 20 ml subsamples (as described above), preserved in 0.4% paraformaldehyde (final concentration), flash-frozen in liquid nitrogen and transferred to a –80°C freezer until processing. Preserved, frozen FCM samples were thawed in batches, stained with the DNA dye Hoechst 34442 (1 µg ml–1, final concentration) (Campbell &amp; Vaulot 1993, Monger &amp; Landry 1993), and analyzed using a Beckman-Coulter Altra flow cytometer for phytoplankton population abundances using fluorescence signals from DNA, phycoerythrin and chl a. Data were grouped into relevant populations using FlowJo (Treestar). PEUK cell abundances were converted to biomass using data from parallel microscopy samples, which showed that the eukaryotic phytoplankton in these samples were dominated by 2 to 3 µm ESD spherical cells, with an average biomass of 1.55 pg C cell–1 (biomass conversions as in Menden-Deuer &amp; Lessard 2000).</p>
<p><strong>Nano- and </strong><strong>microplankton</strong><strong> abundance and biomass (microscopy)</strong></p>
<p>Initial and final samples for nano- and microplankton abundance by epifluorescence microscopy (EPI) were preserved (0.4% paraformaldehyde, final concentration), and kept in the dark and cold (4°C) until filtered within 24 to 48 h. EPI samples (25 or 50 ml) were stained with 0.5 nM proflavin (1 to 2 h prior to filtration), then filtered onto 0.8 µm black polycarbonate filters (Midland Scientific), stained with 4’,6-diamidino-2-phenylindole (DAPI) for 2 min and mounted on a slide. These slides were frozen at –80°C until digitally imaged within 2 mo of collection.</p>
<p>Digital images of the slides were taken using a color camera (Olympus U-LH100HGAPO) attached to an epifluorescence microscope (Olympus Model BX51 TRF, 400× total magnification), and the software program MicrofireTM (Optronics). For each slide, 3 sequential digital images were taken of 30 random fields, using 3 different excitation/emission filters; one each to illuminate chl a/proflavin (EX450-480; DM500, EM≥515), phycoerythrin (primarily due to Synechococcus), and DNA (EX330-385, DM400, EM&gt;420) fluorescence. Living cells were distinguished from dead cells and debris by the presence of nuclei, and autotrophic and heterotrophic cells were distinguished by the presence of chl a.</p>
<p>Images were analyzed by sizing, counting, and identifying autotrophic and heterotrophic cells 2–10 µm in size until &gt;100 cells were characterized. &nbsp;To evaluate the large (&gt;10 µm) cell abundance and biomass, all &gt;10 µm cells on ¼ of one randomly selected control and treatment slide from each experiment were counted, identified as an autotroph or heterotroph, and measured using a calibrated ocular micrometer. Cell dimensions were used to estimate biovolume (oblate spheroid), and converted to biomass (Menden-Deuer &amp; Lessard 2000). Diatoms &gt;10 µm were also quantified, but they were never abundant during our experiments (see Table 2).</p>
<p>Ciliate biomass and abundance was estimated by inverted microscopy on samples pre-screened through a &lt;35 µm mesh, preserved with a 1/20 dilution of acid Lugol’s solution (Throndsen 1978), and kept in the dark at room temperature until analysis (~1 yr later) by the Utermöhl technique (Sherr &amp; Sherr 1993). Aliquots of 28 ml from randomly selected control and experimental treatments were settled and their entire contents examined (18 to 87 total cells in sample volume, median 55) with a Zeiss inverted microscope (400× magnification), with digital images taken (Moticam camera and software) for subsequent dimensional analyses. The measured length and width of each cell was converted to biovolume based on the appropriate geometric shapes, and converted to carbon biomass (Menden-Deuer &amp; Lessard 2000).</p>
<p><strong>Chl a determinations</strong></p>
<p>For chl a, triplicate 305 ml samples were filtered onto GF/Fs (Whatman), flash-frozen (LN2), and kept at –80°C freezer until measurements were made 4 mo later. Chl a (and phaeopigment) was measured using a Turner Designs (model 10AU) fluorometer, using the standard extraction and acidification technique (Yentsch &amp; Menzel 1963, Strickland &amp; Parsons 1972).</p>
<p>For complete methodology, see the Supplemental Files section.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1255697 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1255697
completed
Erica Goetze
University of Hawaiʻi at Mānoa
808-956-7156
Department of Oceanography, University of Hawaii at Manoa 1000 Pope Road
Honolulu
HI
96822
United States
egoetze@hawaii.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
experiment
date_local
lat
lon
prey_quant_method
prey_type_size_range
initial_cells
initial_biomass
Beckman Coulter Multisizer III Coulter Counter
Beckman Coulter Altra flow cytometer
theme
None, User defined
experiment id
date_local
latitude
longitude
sampling_method
length_range
abundance
wet_weight_biomass
featureType
BCO-DMO Standard Parameters
Coulter Counter
Flow Cytometer
Fluorescence Microscope
Inverted Microscope
Turner Designs Fluorometer 10-AU
instrument
BCO-DMO Standard Instruments
Goetze_2012-2013
service
Deployment Activity
Kaneohe Bay, HI
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
EAGER: New molecular methods for studying copepod nauplii in the field
https://osprey.bco-dmo.org/project/473049
EAGER: New molecular methods for studying copepod nauplii in the field
<p><em>Description from NSF Award Abstract:</em><br />
The most abundant metazoans in the open sea are often the earliest developmental stages of copepods, their nauplii. Nauplii remain under-studied due to the limitations of conventional techniques and an historical emphasis on studying the larger mesozooplankton. However, there is increasing recognition that nauplii play important roles in food web dynamics, and considerable evidence that nauplii may be important trophic intermediaries between microbial and classical food webs due to their high abundance, high weight-specific ingestion rates, and ability to feed on relatively small particles. This team of investigators is developing a novel molecular approach to studying diverse populations of nauplii in mixed field samples based on quantitative Polymerase Chain Reaction (qPCR). They propose to complete development and validation of this qPCR-based technique for enumeration of nauplii, and evaluate its utility in the field. The specific objectives of this research are to identify and reduce technical and biological sources of error in the methodology, determine the accuracy of the method across a range of environmental conditions, and complete one paired field experiment that compares the grazing impact of naupliar and protozoan micro-grazers in a model subtropical coastal ecosystem.</p>
<p><strong>Note:</strong> This project is funded by an NSF EAGER award.</p>
<p><em>Related publications:</em><br />
Jungbluth, M.J., Goetze, E., and Lenz, P.H. 2013. Measuring copepod naupliar abundance in a subtropical bay using quantitative PCR. Marine Biology, 160: 3125-3141. doi: <a href="http://dx.doi.org/10.1007/s00227-013-2300-y" target="_blank">10.1007/s00227-013-2300-y</a></p>
<p>Jungbluth, M.J., and Lenz, P.H. 2013. Copepod diversity in a subtropical bay based on a fragment of the mitochondrial COI gene. Journal of Plankton Research, 35(3): 630-643. doi: <a href="http://dx.doi.org/10.1093/plankt/fbt015" target="_blank">10.1093/plankt/fbt015</a></p>
EAGER: Copepod nauplii
largerWorkCitation
project
eng; USA
biota
oceans
Kaneohe Bay, HI
-157.78
-157.78
21.432
21.432
2013-05-27
2013-06-05
Kaneohe Bay, Oahu, Hawaii
0
BCO-DMO catalogue of parameters from Initial prey abundances for copepod grazing experiments in the Kaneohe Bay, HI, May-June 2013 (MEPS 2017) (EAGER: Copepod nauplii project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/712643.rdf
Name: experiment
Units: unitless
Description: <p>experiment number</p>
http://lod.bco-dmo.org/id/dataset-parameter/712644.rdf
Name: date_local
Units: unitless
Description: <p>local date formatted as yyyy-mm-dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/712645.rdf
Name: lat
Units: decimal degrees
Description: <p>latitude; north is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/712646.rdf
Name: lon
Units: decimal degrees
Description: <p>longitude; east is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/712647.rdf
Name: prey_quant_method
Units: unitless
Description: <p>Method used to determine abundance or biomass of prey:<br />
CC=Coulter counter measured prey;<br />
CHL=Chlorophyll a (<35 µm);<br />
FCM=Flow cytometry measured;<br />
EPI=Epifluorescence microscopy measured;<br />
INVERTED=Lugol’s preserved and inverted microscopy measured</p>
http://lod.bco-dmo.org/id/dataset-parameter/712648.rdf
Name: prey_type_size_range
Units: micrometers
Description: <p>type or size range of prey items:<br />
PEUK=photosynthetic eukaryote<br />
AUT=autotrophic<br />
HET=heterotrophic<br />
SYN=Synechococcus<br />
PRO=Prochlorococcus<br />
HBACT=Heterotrophic bacteria</p>
http://lod.bco-dmo.org/id/dataset-parameter/712649.rdf
Name: initial_cells
Units: cells/milliliter
Description: <p>initial concentration of prey cells</p>
http://lod.bco-dmo.org/id/dataset-parameter/712650.rdf
Name: initial_biomass
Units: micrograms Carbon/Liter or micrograms Chlorophyll/Liter
Description: <p>initial biomass of prey cells measured as either carbon or chlorophyll</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
4365
https://darchive.mblwhoilibrary.org/bitstream/1912/26599/1/dataset-712626_initial-prey-abundance-and-biomass-meps-2017__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.712626.1
download
onLine
dataset
<p>From Jungbluth et al. 2017 – MEPS</p>
<p><strong>Prey size spectra and abundance (Coulter Counter)</strong></p>
<p>Initial and final time-point Coulter Counter (CC) samples were taken for prey particle spectra by gently pouring 20 ml from each incubation bottle through a 35 µm cap filter into a clean beaker, then gently back-washing the filter into the experimental bottle to return any nauplii using a small volume of 0.2 µm filtered seawater. From this subsample, triplicate 2 ml volumes were measured with a Beckman Coulter Multisizer III CC with a 100 µm orifice tube, yielding a spectrum of particle sizes from 2–35 µm ESD, as well as quantitative abundance data. These raw data were further processed in R (R Core Team 2016) to streamline binning of prey size groups, for calculations of clearance and ingestion rates, and for statistical analyses.</p>
<p>Prey ESD was converted to biovolume (BV; µm 3), then to carbon (C; pg C cell–1), using the relationship C = 0.216 × BV0.939, which applies well to taxonomically diverse protists (Menden-Deuer &amp; Lessard 2000). Averages of cell abundance and biomass were binned into 5 prey size groupings (2–5, 5–10, 10–15, 15–20, and 20–35 µm). The binned, averaged data for initial and final time points for each control and treatment bottle were used to calculate carbon ingestion (I; ng C nauplius–1 h–1) and clearance rates (F, ml nauplius–1 h–1) on each prey size group using the equations of Frost (1972).</p>
<p><strong>Photosynthetic eukaryotes (flow cytometry)</strong></p>
<p>Flow cytometry (FCM) samples (1.5 ml) for photosynthetic eukaryote (PEUK) abundance were taken from the 20 ml subsamples (as described above), preserved in 0.4% paraformaldehyde (final concentration), flash-frozen in liquid nitrogen and transferred to a –80°C freezer until processing. Preserved, frozen FCM samples were thawed in batches, stained with the DNA dye Hoechst 34442 (1 µg ml–1, final concentration) (Campbell &amp; Vaulot 1993, Monger &amp; Landry 1993), and analyzed using a Beckman-Coulter Altra flow cytometer for phytoplankton population abundances using fluorescence signals from DNA, phycoerythrin and chl a. Data were grouped into relevant populations using FlowJo (Treestar). PEUK cell abundances were converted to biomass using data from parallel microscopy samples, which showed that the eukaryotic phytoplankton in these samples were dominated by 2 to 3 µm ESD spherical cells, with an average biomass of 1.55 pg C cell–1 (biomass conversions as in Menden-Deuer &amp; Lessard 2000).</p>
<p><strong>Nano- and </strong><strong>microplankton</strong><strong> abundance and biomass (microscopy)</strong></p>
<p>Initial and final samples for nano- and microplankton abundance by epifluorescence microscopy (EPI) were preserved (0.4% paraformaldehyde, final concentration), and kept in the dark and cold (4°C) until filtered within 24 to 48 h. EPI samples (25 or 50 ml) were stained with 0.5 nM proflavin (1 to 2 h prior to filtration), then filtered onto 0.8 µm black polycarbonate filters (Midland Scientific), stained with 4’,6-diamidino-2-phenylindole (DAPI) for 2 min and mounted on a slide. These slides were frozen at –80°C until digitally imaged within 2 mo of collection.</p>
<p>Digital images of the slides were taken using a color camera (Olympus U-LH100HGAPO) attached to an epifluorescence microscope (Olympus Model BX51 TRF, 400× total magnification), and the software program MicrofireTM (Optronics). For each slide, 3 sequential digital images were taken of 30 random fields, using 3 different excitation/emission filters; one each to illuminate chl a/proflavin (EX450-480; DM500, EM≥515), phycoerythrin (primarily due to Synechococcus), and DNA (EX330-385, DM400, EM&gt;420) fluorescence. Living cells were distinguished from dead cells and debris by the presence of nuclei, and autotrophic and heterotrophic cells were distinguished by the presence of chl a.</p>
<p>Images were analyzed by sizing, counting, and identifying autotrophic and heterotrophic cells 2–10 µm in size until &gt;100 cells were characterized. &nbsp;To evaluate the large (&gt;10 µm) cell abundance and biomass, all &gt;10 µm cells on ¼ of one randomly selected control and treatment slide from each experiment were counted, identified as an autotroph or heterotroph, and measured using a calibrated ocular micrometer. Cell dimensions were used to estimate biovolume (oblate spheroid), and converted to biomass (Menden-Deuer &amp; Lessard 2000). Diatoms &gt;10 µm were also quantified, but they were never abundant during our experiments (see Table 2).</p>
<p>Ciliate biomass and abundance was estimated by inverted microscopy on samples pre-screened through a &lt;35 µm mesh, preserved with a 1/20 dilution of acid Lugol’s solution (Throndsen 1978), and kept in the dark at room temperature until analysis (~1 yr later) by the Utermöhl technique (Sherr &amp; Sherr 1993). Aliquots of 28 ml from randomly selected control and experimental treatments were settled and their entire contents examined (18 to 87 total cells in sample volume, median 55) with a Zeiss inverted microscope (400× magnification), with digital images taken (Moticam camera and software) for subsequent dimensional analyses. The measured length and width of each cell was converted to biovolume based on the appropriate geometric shapes, and converted to carbon biomass (Menden-Deuer &amp; Lessard 2000).</p>
<p><strong>Chl a determinations</strong></p>
<p>For chl a, triplicate 305 ml samples were filtered onto GF/Fs (Whatman), flash-frozen (LN2), and kept at –80°C freezer until measurements were made 4 mo later. Chl a (and phaeopigment) was measured using a Turner Designs (model 10AU) fluorometer, using the standard extraction and acidification technique (Yentsch &amp; Menzel 1963, Strickland &amp; Parsons 1972).</p>
<p>For complete methodology, see the Supplemental Files section.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- reformatted date from d-Mon-yy to yyyy-mm-dd</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Beckman Coulter Multisizer III Coulter Counter
Beckman Coulter Multisizer III Coulter Counter
PI Supplied Instrument Name: Beckman Coulter Multisizer III Coulter Counter PI Supplied Instrument Description:100 µm orifice tube Instrument Name: Coulter Counter Instrument Short Name: Instrument Description: An apparatus for counting and sizing particles suspended in electrolytes. It is used for cells, bacteria, prokaryotic cells and virus particles. A typical Coulter counter has one or more microchannels that separate two chambers containing electrolyte solutions.
from https://en.wikipedia.org/wiki/Coulter_counter
Beckman Coulter Altra flow cytometer
Beckman Coulter Altra flow cytometer
PI Supplied Instrument Name: Beckman Coulter Altra flow cytometer Instrument Name: Flow Cytometer Instrument Short Name:Flow Cytometer Instrument Description: Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/
PI Supplied Instrument Name: Instrument Name: Fluorescence Microscope Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB06/
PI Supplied Instrument Name: Instrument Name: Inverted Microscope Instrument Short Name: Instrument Description: An inverted microscope is a microscope with its light source and condenser on the top, above the stage pointing down, while the objectives and turret are below the stage pointing up. It was invented in 1850 by J. Lawrence Smith, a faculty member of Tulane University (then named the Medical College of Louisiana).
Inverted microscopes are useful for observing living cells or organisms at the bottom of a large container (e.g. a tissue culture flask) under more natural conditions than on a glass slide, as is the case with a conventional microscope. Inverted microscopes are also used in micromanipulation applications where space above the specimen is required for manipulator mechanisms and the microtools they hold, and in metallurgical applications where polished samples can be placed on top of the stage and viewed from underneath using reflecting objectives.
The stage on an inverted microscope is usually fixed, and focus is adjusted by moving the objective lens along a vertical axis to bring it closer to or further from the specimen. The focus mechanism typically has a dual concentric knob for coarse and fine adjustment. Depending on the size of the microscope, four to six objective lenses of different magnifications may be fitted to a rotating turret known as a nosepiece. These microscopes may also be fitted with accessories for fitting still and video cameras, fluorescence illumination, confocal scanning and many other applications. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB05/
PI Supplied Instrument Name: Instrument Name: Turner Designs Fluorometer 10-AU Instrument Short Name:Turner Fluorometer 10-AU Instrument Description: The Turner Designs 10-AU Field Fluorometer is used to measure Chlorophyll fluorescence. The 10AU Fluorometer can be set up for continuous-flow monitoring or discrete sample analyses. A variety of compounds can be measured using application-specific optical filters available from the manufacturer (read more from Turner Designs, turnerdesigns.com, Sunnyvale, CA, USA). Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0393/
Deployment: Goetze_2012-2013
Goetze_2012-2013
lab UHawaii_SOEST
laboratory
Goetze_2012-2013
Erica Goetze
University of Hawaiʻi at Mānoa
lab UHawaii_SOEST
laboratory