<div><p>Collection of Orbicella faveolata larvae was made at Alligator Reef (24°48.7710 N, 80°40.1670 W) and Looe Key (24°32.6930 N, 81°24.5620 W) in 2011. Initial rearing was done at Keys Marine Laboratory (KML), Long Key, FL. Settled corals were then maintained at University of Miami Experimental Hatchery (Virginia Key, Miami, FL). <em>Symbiodinium</em> cultures originated from highly concentrated, isoclonal reference cultures maintained at the University at Buffalo – SUNY (BURR Culture Collection: <a href="http://www.nsm.buffalo.edu/Bio/burr/" target="_blank">http://www.nsm.buffalo.edu/Bio/burr/</a>). The cultures used in the experiments were maintained at the Keys Marine Lab (Florida) in f/2 medium (Guillard 1975), at ~27°C, under a 14:10 h light:dark regime.</p>
<p>Measures of photophysiology for recruits harboring either S. microadriaticum or S. minutum were determined using an imaging-PAM (WALZ Mess- und Regeltechnik, Germany). All fluorescence parameters were described and abbreviated according to Cosgrove and Borowitzka (2010) and Enriquez and Borowitzka (2010). For the first 6 months following settlement (age in months 1-6) analyses were run monthly on one designated tile per tank (n = 4 tiles). We measured the effective quantum yield (delta Fv/Fm0', the efficiency of photochemistry in the light-adapted state) for six randomly selected recruits from each tile repeated monthly for 6 months. Data were analyzed using a two-way ANOVA, with symbiont treatment and time as factors and tile nested within treatment.</p>
<p>Additionally, RLC (rapid light curves) analysis was performed on 2-month-old recruits. RLC analysis measured deltaFv/ Fm' as a function of increasing irradiance, in this case across eight steps of increasing PAR (0, 11, 21, 36, 56, 81, 111, and 146 umol photons/m2/s), each of which was followed by a saturating pulse. RLC analysis examines the way in which algae use light to drive photosynthesis, by converting deltaFv/ Fm' into a measure of the rate of electron transport through the photosynthetic chain (rETR). Using SigmaPlot (Systat Software, San Jose, CA, USA), we fitted a hyperbolic tangent function (Platt et al. 1980) to the PAR versus rETR data for each of 48 recruits (2 Symbiodinium treatments x 4 replicate tanks x 6 polyps), and calculated the maximum light utilization coefficient (alpha, the slope of the linear phase at subsaturating light). We used a two-tailed Student's t-test to test for a significant effect of Symbiodinium treatment on alpha.</p></div>
McIlroy_2016: Maximum quantum yield for Orbicella faveolata recruits
<div><p>Maximum quantum yield data for <em>Orbicella faveolata</em> recruits inoculated with either <em>Symbiodinium microadriaticum</em> or <em>Symbiodinium minutum </em>and maintained in the laboratory for monitoring. These data were used in McIlroy et al (2016).</p>
<p><strong>Methods, data processing, and results reported in:</strong><br />
McIlroy SE, Gillette P, Cunning R, Klueter A, Capo T, Baker AC, Coffroth MA (2016) The effects of Symbiodinium (Pyrrhophyta) identity on growth, survivorship, and thermal tolerance of newly settled coral recruits. Journal of Phycology 52:1114–1124. DOI: <a href="http://onlinelibrary.wiley.com/doi/10.1111/jpy.12471/epdf" target="_blank">10.1111/jpy.12471</a></p>
<p><strong>Related Datasets:</strong><br /><a href="https://www.bco-dmo.org/dataset/714350">McIlroy_2016: Growth of Orbicella faveolata recruits</a><br /><a href="https://www.bco-dmo.org/dataset/714395">McIlroy_2016: Effective quantum yield for Orbicella faveolata recruits</a><br /><a href="https://www.bco-dmo.org/dataset/714433">McIlroy_2016: Symbionts genotypes in Orbicella faveolata</a></p></div>
McIlroy_2016: Maximum quantum yield for Orbicella faveolata recruits
<div><p><strong>BCO-DMO Processing:</strong><br />
- original file: DATASET_McIlroyetal2016_Orbicella Maximum Quantum Yield.xlsx<br />
- modified conventional header with dataset name, PI name, version date, reference information<br />
- changed parameter names to be BCO-DMO compatible</p></div>
714412
McIlroy_2016: Maximum quantum yield for Orbicella faveolata recruits
2017-09-07T16:39:25-04:00
2017-09-07T16:39:25-04:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:714412
Laboratory results for maximum quantum yield data for Orbicella faveolata recruits inoculated with either Symbiodinium microadriaticum or S. minutum (McIlroy,J. Phycology, 2016) (SymBioSys project)
false
Coffroth, M. A. (2017) Laboratory results for maximum quantum yield data for Orbicella faveolata recruits inoculated with either Symbiodinium microadriaticum or S. minutum (McIlroy,J. Phycology, 2016) (SymBioSys project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2017-09-07 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/714412 [access date]
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2017-09-07
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