|Passow, Uta||University of California-Santa Barbara (UCSB-MSI)||Principal Investigator|
|Copley, Nancy||Woods Hole Oceanographic Institution (WHOI BCO-DMO)||BCO-DMO Data Manager|
Experiments were conducted to investigate the combined effect of light and temperature changes on the growth rate (mu) of Thalassiosira pseudonana CCMP 1335. T. pseudonana was grown in artificial seawater (ASW) (Kester et al.1967), enriched as in f/2 (Guillard 1975). Each of five experiments per series was conducted at a different temperature (13.4; 18.5; 24.4; 26.5; 22.5 C). At each temperature, cultures were kept on a 12-hour light 12-hour dark cycle under eight light intensities ranging from 35 umol/m^2/S to 140 umol/m^2/s. Optical density measurements (OD680 and OD720), dark-adapted Instantaneous Chlorophyll Fluorescence (F0) and the quantum yield (QY=Fv/Fm, where Fv is the maximal variable fluorescence and Fm is the maximal fluorescence intensity) and cell concentrations were determined daily at the end of the dark period. Cell counts were conducted in a hemocytometer on a microscope.
To measure dark adapted instantaneous chlorophyll fluorescence (F0) and quantum yield (QY=Fv/Fm, where Fv is the maximal variable fluorescence and Fm is the maximal fluorescence intensity) dark adaptation time needs to be determined. Thalassiosira pseudonana CCMP 1335 is extremely sensitive to small amounts of even green or red light and a successful curve can only be conducted in complete darkness. Dark adaptation times were tested over a 30-minutes period.
BCO-DMO Processing Notes:
- added conventional header with dataset name, PI name, version date
- modified parameter names to conform with BCO-DMO naming conventions
|minutes||elapsed time between measurements||minutes|
|F0_replicate1||Instantaneous chlorophyll fluorescence (F0)- replicate 1||relative units|
|F0_replicate2||Instantaneous chlorophyll fluorescence (F0) - replicate 2||relative units|
|F0_replicate3||Instantaneous chlorophyll fluorescence (F0) - replicate 1||relative units|
|Dataset-specific Instrument Name|| |
Z985 Cuvette Aquapen (Qubit Systems)
|Generic Instrument Name|| |
|Dataset-specific Description|| |
Used to measure instantaneous chlorophyll fluorescence (F0). AquaPen settings: f = 30, F=71, A = 50.
|Generic Instrument Description|| |
A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.