http://lod.bco-dmo.org/id/dataset/714942
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2017-09-15
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Feeding trials: Effects of diversity in feeding trials, conducted at Bodgea Marine Laboratory, using detritus from eelgrass (Zostera marina) genotypes (clones) as a food source and either one or a combination of invertebrate grazers
2017-09-15
publication
2017-09-15
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-08-02
publication
https://doi.org/10.1575/1912/bco-dmo.714942.1
John J. Stachowicz
University of California-Davis
principalInvestigator
Richard K. Grosberg
University of California-Davis
principalInvestigator
Susan L. Williams
University of California - Davis: Bodega Marine Laboratory
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Stachowicz, J., Grosberg, R., Williams, S. (2017) Feeding trials: Effects of diversity in feeding trials, conducted at Bodgea Marine Laboratory, using detritus from eelgrass (Zostera marina) genotypes (clones) as a food source and either one or a combination of invertebrate grazers. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2017-09-15 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.714942.1 [access date]
Feeding trials using detritus from eelgrass (Zostera marina) genotypes (clones) as a food source and either one or a combination of invertebrate grazers Dataset Description: <p>In this project, we examined the effect of eelgrass genetic and invertebrate species diversity on detrital consumption and animal survival rates in a series of laboratory experiments. This dataset contains chemical traits for individual eelgrass clones and feeding rates for each grazer type (isopods, amphipods, polychaetes).</p>
<p>Abstract:<br />
Seagrass meadows are among the world's most productive ecosystems, and as in many other systems, genetic diversity is correlated with increased production. However, only a small fraction of seagrass production is directly consumed, and instead much of the secondary production is fueled by the detrital food web. Here, we study how plant genotype influences detrital consumption. We used three common mesograzers—an amphipod,&nbsp;<em>Ampithoe lacertosa</em>, an isopod<em>, Idotea resecata</em>, and a polychaete,&nbsp;<em>Platynereis bicanaliculata</em>. Each grazer consumed eelgrass detritus at rates greater than live eelgrass or macroalgae. This detrital consumption, however, was not spread evenly over leaves shed from different eelgrass clones.&nbsp;Palatability and consumption varied because of genotype specific differences in leaf texture, secondary metabolites (phenolics), and nutritional quality (nitrogen). Further, detritus derived from some eelgrass genotypes was palatable to all grazers, while detritus from other genotypes was preferentially consumed by only one grazer species.</p>
<p>These data are illustrated in figures 2 and 3 of the manuscript:<br />
Reynolds LK, KM Chan, E Huynh, SL Williams, and JJ Stachowicz (in press) Plant genotype indentity and diversity interact with mesograzer species diversity to influence detrital consumption in eelgrass meadows.&nbsp;DOI:<a href="http://dx.doi.org/10.1111/oik.04471" target="_blank">10.1111/oik.04471</a></p> Methods and Sampling: <p>We conducted a series of food choice experiments using detritus from cultured eelgrass (Zostera marina) genotypes (clones) as a food source and either one or a combination of the following invertebrate grazers: the tube dwelling amphipod <em>Ampithoe lacertosa</em>, the free swimming isopod <em>Idotea resecata</em>, and/or the tube building polychaete <em>Platynereis bicanaliculata</em>.</p>
<p>All feeding trials were conducted by placing pre-weighed fragments of each choice (approximately 4 cm in length) in 140 mL cups (7 cm tall, 6 cm diameter) covered with a 250 um mesh cloth and submerged in a flowing seawater bath in an indoor tank. Food choices were marked using colored zip ties, and trials were terminated before any food item was reduced in size by one half. Consumption was calculated as ([H<sub>i</sub> X C<sub>f</sub>/C<sub>i</sub>] - H<sub>f</sub> ), where H<sub>i</sub> and H<sub>f</sub> were initial and final wet masses of tissue exposed to consumers, and C<sub>i</sub> and C<sub>f</sub> were initial and final masses in controls.</p>
<p>In addition to feeding trials, we grew invertebrates for one month (in similar containers and feeding trial conditions) with food sources that varied in number of seagrass clones present. Animal survival was assessed weekly, and food was replaced.</p>
<p>The chemical traits for individual eelgrass clones were also assessed. We measured the pressure required to penetrate and tear each genotype. We clamped in place below a needle (17G / 19mm length), which was held in place with a metal sleeve and which supported a cup to which dry sand was added a few milligrams at a time until the pin pierced completely through the plant tissue. The mass of the dry sand and the apparatus were then weighed to determine the mass needed to pierce the leaf (Duffy &amp; Hay 1991). Tensile strength was measured using a tensiometer. Leaf segments were clamped to a hanging balance equipped with a maximum mass indicator and pulled by hand until the leaf failed. Phenolic content was determined on an approximately 4 mg subsample using a modified Folin-Ciocalteu method (see Bolser et al. 1998). An approximately 3 mg subsample was analyzed for carbon and nitrogen concentration on a Thermo Flash EA 1112 Soil elemental analyzer.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1234345 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1234345
completed
John J. Stachowicz
University of California-Davis
530-752-1113
Department of Evolution and Ecology 1 Shields Ave
Davis
CA
95616
USA
jjstachowicz@ucdavis.edu
pointOfContact
Richard K. Grosberg
University of California-Davis
Department of Ecology and Evolution 1 Shields Ave
Davis
CA
95616
USA
rkgrosberg@ucdavis.edu
pointOfContact
Susan L. Williams
University of California - Davis: Bodega Marine Laboratory
707-875-1950
Bodega Marine Laboratory P.O. Box 247
Bodega Bay
CA
94923
USA
slwilliams@ucdavis.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Genotype
Nitrogen_pcnt
Carbon_pcnt
Tear_g
Penetrate_g
Phenolic_pcnt
Isopod_feeding_rate_mg_day
Amphipod_feeding_rate_mg_day
Polychaete_feeding_rate_mg_day
indoor tank
Thermo Flash EA 1112 Soil elemental analyzer
theme
None, User defined
sample description
Nitrogen
Elemental carbon (C)
mass
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
Aquarium
Elemental Analyzer
instrument
BCO-DMO Standard Instruments
BML_Stachowicz
service
Deployment Activity
Bodega Marine Lab
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Connecting genetic diversity to ecosystem functioning: links between genetic diversity, relatedness and trait variation in a seagrass community
https://osprey.bco-dmo.org/project/564447
Connecting genetic diversity to ecosystem functioning: links between genetic diversity, relatedness and trait variation in a seagrass community
<p>There is growing evidence that genetic variation within and among populations of key species plays an important role in marine ecosystem processes. Several experiments provide compelling evidence that the number of genotypes in an assemblage (genotypic richness) can influence critical ecosystem functions including productivity, resistance to disturbance and invasion or colonization success. However, these studies use only the number of genotypes as a measure of genetic diversity. Recent analyses of species diversity experiments show that phylogenetic diversity may be a more reliable predictor of ecosystem functioning than simply the number of species. However, such approaches have not yet been applied to understanding the effects of genetics on ecosystem functioning. While genetic relatedness within a species holds the potential to predict the outcome of intraspecific interactions, and the functioning of ecosystems that depend on those species, we currently have few data to assess the shape or strength of this relationship. The investigators will build on their own previous work, and that of others, in eelgrass (<em>Zostera marina</em>) ecosystems showing strong effects of genotypic richness on a spectrum of critical ecosystem processes. The investigators will ask whether genotypic richness, or - as in studies at the level of species diversity - genetic relatedness/distance better predicts ecosystem functioning? If genetic relatedness measures are better predictors, then what mechanisms underlie this relationship? Can genetic relatedness predict ecological relatedness?</p>
<p>Although the current focus is on eelgrass, the research should be applicable to many systems. The project will assess the relationship between genetic relatedness and phenotypic distinctiveness of a key marine foundation species and use manipulative experiments to test the relative importance of the number of genotypes in an assemblage vs. their genetic relatedness and trait diversity for ecosystem functioning. Specifically, experiments will:<br />
(1) characterize the relationship between genetic relatedness and trait similarity among individual genotypes of eelgrass, including responses to experimental warming;<br />
(2) compare the effects of genetic relatedness and trait similarity among genotypes on the outcome of intraspecific competitive interactions; and<br />
(3) test the relative effect of genetic relatedness vs. number of genotypes of eelgrass on the growth of eelgrass, its associated ecosystem functions it (e.g., primary production, nutrient dynamics, trophic transfer, habitat provision, and detrital production and decomposition).</p>
<p>Seagrass ecosystems provide important services to coastal regions including primary production, nutrient cycling, habitat for fisheries species, and erosion control. Previous studies have shown these services can be compromised by reduction in the numbers of species of grazers or genotypes, but this study will allow a more predictive approach to diversity loss by integrating the effects of multiple components of diversity and clarifying the extent to which diversity effects can be predicted by the genetic or ecological uniqueness of component genotypes.</p>
Genetic Div to Ecosys Functioning
largerWorkCitation
project
eng; USA
biota
oceans
Bodega Marine Lab
2017-09-15
0
BCO-DMO catalogue of parameters from Feeding trials: Effects of diversity in feeding trials, conducted at Bodgea Marine Laboratory, using detritus from eelgrass (Zostera marina) genotypes (clones) as a food source and either one or a combination of invertebrate grazers
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/715149.rdf
Name: Genotype
Units: unitless
Description: <p>Individual clone</p>
http://lod.bco-dmo.org/id/dataset-parameter/715150.rdf
Name: Nitrogen_pcnt
Units: percent (%)
Description: <p>Detrital leaf nitrogen content</p>
http://lod.bco-dmo.org/id/dataset-parameter/715151.rdf
Name: Carbon_pcnt
Units: percent (%)
Description: <p>Detrital leaf carbon content</p>
http://lod.bco-dmo.org/id/dataset-parameter/715152.rdf
Name: Tear_g
Units: grams (g)
Description: <p>The mass recordered by a tensiometer reqired to break a detrial leaf</p>
http://lod.bco-dmo.org/id/dataset-parameter/715153.rdf
Name: Penetrate_g
Units: grams (g)
Description: <p>The mass needed to push a needle through a detrial leaf</p>
http://lod.bco-dmo.org/id/dataset-parameter/715154.rdf
Name: Phenolic_pcnt
Units: percent (%)
Description: <p>Phenolic concentration measured by the Folin Ciocalteu method</p>
http://lod.bco-dmo.org/id/dataset-parameter/715155.rdf
Name: Isopod_feeding_rate_mg_day
Units: milligrams per day (mg/day)
Description: <p>Isopod (Idotea rescata) feeding rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/715156.rdf
Name: Amphipod_feeding_rate_mg_day
Units: milligrams per day (mg/day)
Description: <p>Amphipod (Amphithoe lacertosa) feeding rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/715157.rdf
Name: Polychaete_feeding_rate_mg_day
Units: milligrams per day (mg/day)
Description: <p>Polychaete (Platynereis bicanicalulata) feeding rate</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
492
https://darchive.mblwhoilibrary.org/bitstream/1912/24397/1/dataset-714942_feeding-trials__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.714942.1
download
onLine
dataset
<p>We conducted a series of food choice experiments using detritus from cultured eelgrass (Zostera marina) genotypes (clones) as a food source and either one or a combination of the following invertebrate grazers: the tube dwelling amphipod <em>Ampithoe lacertosa</em>, the free swimming isopod <em>Idotea resecata</em>, and/or the tube building polychaete <em>Platynereis bicanaliculata</em>.</p>
<p>All feeding trials were conducted by placing pre-weighed fragments of each choice (approximately 4 cm in length) in 140 mL cups (7 cm tall, 6 cm diameter) covered with a 250 um mesh cloth and submerged in a flowing seawater bath in an indoor tank. Food choices were marked using colored zip ties, and trials were terminated before any food item was reduced in size by one half. Consumption was calculated as ([H<sub>i</sub> X C<sub>f</sub>/C<sub>i</sub>] - H<sub>f</sub> ), where H<sub>i</sub> and H<sub>f</sub> were initial and final wet masses of tissue exposed to consumers, and C<sub>i</sub> and C<sub>f</sub> were initial and final masses in controls.</p>
<p>In addition to feeding trials, we grew invertebrates for one month (in similar containers and feeding trial conditions) with food sources that varied in number of seagrass clones present. Animal survival was assessed weekly, and food was replaced.</p>
<p>The chemical traits for individual eelgrass clones were also assessed. We measured the pressure required to penetrate and tear each genotype. We clamped in place below a needle (17G / 19mm length), which was held in place with a metal sleeve and which supported a cup to which dry sand was added a few milligrams at a time until the pin pierced completely through the plant tissue. The mass of the dry sand and the apparatus were then weighed to determine the mass needed to pierce the leaf (Duffy &amp; Hay 1991). Tensile strength was measured using a tensiometer. Leaf segments were clamped to a hanging balance equipped with a maximum mass indicator and pulled by hand until the leaf failed. Phenolic content was determined on an approximately 4 mg subsample using a modified Folin-Ciocalteu method (see Bolser et al. 1998). An approximately 3 mg subsample was analyzed for carbon and nitrogen concentration on a Thermo Flash EA 1112 Soil elemental analyzer.</p>
Specified by the Principal Investigator(s)
<p>BCO-DMO Processing:<br />
- separated original Excel file into 3 datasets: feeding trials, biodiversity experiments, invertebrate survival;<br />
- modified parameter names to conform with BCO-DMO naming conventions: replaced % with pcnt, replaced spaces with underscores.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
indoor tank
indoor tank
PI Supplied Instrument Name: indoor tank PI Supplied Instrument Description:Pre-weighed fragments of eelgrass were covered with a 250 um mesh cloth and submerged in a flowing seawater bath in an indoor tank. Instrument Name: Aquarium Instrument Short Name:Aquarium Instrument Description: Aquarium - a vivarium consisting of at least one transparent side in which water-dwelling plants or animals are kept
Thermo Flash EA 1112 Soil elemental analyzer
Thermo Flash EA 1112 Soil elemental analyzer
PI Supplied Instrument Name: Thermo Flash EA 1112 Soil elemental analyzer PI Supplied Instrument Description:Carbon and nitrogen concentrations were measured on a Thermo Flash EA 1112 Soil elemental analyzer. Instrument Name: Elemental Analyzer Instrument Short Name: Instrument Description: Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/
Deployment: BML_Stachowicz
BML_Stachowicz
lab Bodega Marine Laboratory
laboratory
BML_Stachowicz
John J. Stachowicz
University of California-Davis
lab Bodega Marine Laboratory
laboratory