<div><p>All samples were processed at UCSB in the Passow Laboratory. Samples for the carbonate system, DOC, and cell concentration were collected first to forestall changes due to bacterial activity and cell sinking. All carbonate system samples were overfilled by a minimum of 50% volume into acid rinsed borosilicate glass bottles leaving ~1% headspace and fixed with 130µL of saturated mercuric chloride solution (Dickson et al., 2007). All samples were stored at 2 degrees C until analysis. DOC samples were gravity-filtered through precombusted 0.2µm GF/F filters into combusted glass vials and acidified with 60µL of 4N HCl. Samples were collected in duplicate for each replicate tank. All samples were refrigerated until analysis. Approximately 20ml of well-mixed background water was collected and fixed with buffered Formalin (10%) to determine cell concentrations. Background tank water was then sampled for inorganic nutrients, POC, TEP, and biogenic silica (bSi). Inorganic nitrate, nitrite, phosphate, and silicate were collected into 20ml HDPE vials and frozen until analysis. Samples for POC were filtered onto precombusted 0.4 GF/F filters, dried at 60ºC for 24h and stored in a desiccator until analysis. TEP samples were filtered onto 0.4lm polycarbonate filters and stained with Alcian Blue following Passow and Alldredge (1995). The Alcian Blue dye was calibrated using Gum Xanthum equivalents per liter. Samples for bSi were filtered through 0.6lm polycarbonate filters and frozen until analysis.</p>
<p>Once the background water was sampled, aggregates were collected from the bottom of the rolling tanks and transferred with minimal surrounding seawater to acid washed 1L polycarbonate bottles. The aggregate slurry was then mixed gently and measured for cell concentration, POC, TEP, and bSi following the same methods outlined above. Due to the time required to sample each tank, samples for both background water and aggregate slurry were sampled for one replicate before moving on to the next. The entire sampling process took approximately 4h per treatment. Tanks were opened and sampled in a 2 degrees C temperature controlled room to slow bacterial remineralization throughout the sampling process.</p></div>
Experiment with the diatom Chaetoceros sp. on the impact of temperature, light climate, and carbonate chemistry on TEP production and aggregation processes
<div><p>In this experiment, we used five-liter rolling tanks to address the question of whether elevated pCO2, temperature, and light climate simulating a future climate scenario will increase the aggregation potential for a phytoplankton clone representing the diatom genus, Chaetoceros. Bloom development, TEP production, and aggregation were monitored over an eight-day period to observe how simulated future ocean conditions may influence bloom dynamics for this species compared to the species’ optimal growth condition.</p>
<p>A freshly isolated species of the phytoplankter genus Chaetoceros (10-50um cell length) was used was isolated in June of 2014 in the Eastern Pacific CCS (38.700N 123.671W). In culture, Chaetoceros sp. grew in f/2 media, over a temperature gradient of 12-25 ºC and light climate ranging from 70-400 µmol m-2s-1.</p>
<p>Two experimental treatments were used to assess the impacts of increased light, temperature, and pCO2 stress on the processes of DIC uptake, TEP production, and aggregation. For each treatment, 12 gas-tight polycarbonate rolling tanks were exposed to a single combination of light climate, temperature, and pCO2 representing either optimal or future conditions. Rolling tanks were constructed and maintained to establish solid body rotation. Target temperature (13 °C) and light intensity (100 µmol m-2s-1) for the optimal treatment were determined in the pre-experimental phase with the addition of present-day levels of pCO2 (400 ppm). In the treatment representing predicted increases in stratification, warming, and elevated pCO2, target future conditions were 18 ºC, 200 µmol m-2s-1, and 800 ppm.</p></div>
Data set 4: TEP production and aggregation of Chaetoceros sp. as a function of light climate, pCO2, and temperature
<div><p>All data were processed using R: version 3.2.4 (2016-03-10) -- "Very Secure Dishes" Copyright (C) 2016 The R Foundation for Statistical Computing Platform: x86_64-apple-darwin13.4.0 (64-bit)</p>
<p><strong>BCO-DMO Processing Notes:</strong></p>
<p>Replaced "NA" with "nd" to be compatible with the BCO-DMO system.</p></div>
715125
Data set 4: TEP production and aggregation of Chaetoceros sp. as a function of light climate, pCO2, and temperature
2017-09-15T15:15:13-04:00
2017-09-15T15:15:13-04:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:715125
Experiment with the diatom Chaetoceros sp. on the impact of temperature, light climate, and carbonate chemistry on TEP production and aggregation processes from May 2015 (OA - Effects of High CO2 project)
false
Jones, J., Passow, U. (2017) Experiment with the diatom Chaetoceros sp. on the impact of temperature, light climate, and carbonate chemistry on TEP production and aggregation processes from May 2015 (OA - Effects of High CO2 project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2017-08-28) Version Date 2017-08-28 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/715125 [access date]
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2017-08-28
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