http://lod.bco-dmo.org/id/dataset/715856
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2017-09-29
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Treatment description and growth parameters for Thalassiosira weissflogii generating d13C-AA, collected on R/V Meteor M77 in the Peruvian ocean margin from November to December 2008.
2017-09-14
publication
2017-09-14
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-03-19
publication
https://doi.org/10.1575/1912/bco-dmo.715856.1
Matthew D. McCarthy
University of California-Santa Cruz
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: McCarthy, M. (2017) Treatment description and growth parameters for Thalassiosira weissflogii generating d13C-AA, collected on R/V Meteor M77 in the Peruvian ocean margin from November to December 2008. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2017-09-14 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.715856.1 [access date]
Algal treatment data and ID abbreviations Dataset Description: <p>Treatment description and growth parameters for Thalassiosira weissflogii generating d13C-AA.</p>
<p>Temperature and irradiance values are means of outdoor conditions; the 25% and 75% quartiles for temperature were 9.8 and 11.3 deg C, and the 25% and 75% quartiles for irradiance levels were 269 and 1274 umol m-2 s-1.</p>
<p><strong>These data were published in:</strong></p>
<p>Larsen T, , L. T. Bach, R. Salvatteci, M. Ventura, Y. V. Wang, N. Andersen, and M. D. McCarthy (2015).&nbsp; Assessing the potential of amino acid d13C patterns as a carbon source tracer in marine sediments: effects of algal growth conditions and sedimentary diagenesis. (Biogeosciences, 12, 4979-4992. doi:<a href="https://www.biogeosciences.net/12/4979/2015/" target="_blank">10.5194/bg-12-4979-2015</a></p> Methods and Sampling: <p><strong>Culturing</strong></p>
<p>The marine diatom Thalassiosira weissflogii Grunow (strain CCMP 1010) was cultured in sterile filtered natural North Sea water (Schleswig-Holstein, Germany) or Baltic Sea water (Schleswig-Holstein, Germany). The medium was enriched with f/4 concentrations of macro- and micronutrients (nitrate, phosphate, silicic acid, trace metal mixture, vitamin mixture (Guillard and Ryther, 1962). All experiments were performed in sterile 2.1 L Schott Duran glass bottles. These bottles were made of borosilicate glass (filters UV radiation &lt;310 nm) except for the quartz glass bottles (pure silica without UV radiation filter) used in the UV experiment. The cultures were either incubated in climate chambers with 400 –700 nm radiation or 10 cm below water level at low tide in Kiel Fjord in May 2011. Water temperature and light irradiance data were obtained from the weather station maintained by the GEOMAR institute in Kiel, Germany. Growth conditions for the various treatments, i.e. salinity, pH, temperature, and irradiance are given in Table 1. pH values (reported on free scale) were measured &nbsp;with separate glass and reference electrodes (Metrohm) and calculated with equation 3 from DOE 2007 chapter 6b (Dickson et al., 2007) as described in (Bach et al., 2012). Cultures were inoculated with densities of 20 cells ml-1. &nbsp;Cell densities and equivalent spherical diameters were determined with a Coulter Counter (Beckman Coulter) at the beginning and the end of the experiment, respectively. When incubations ended, cells were filtered on 47 mm diameter, 5 um mesh size Nucleopore Track-Etch Membrane filters (Whatman) and frozen at -18 deg C immediately after filtration.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1131816 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1131816
completed
Matthew D. McCarthy
University of California-Santa Cruz
831-459-1533
1156 High Street
Santa Cruz
CA
95064
USA
mdmccar@ucsc.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
ID
Treatment
Temp
Light
Light_cycle
Salinity
pH_init
pH_term
Light meter
pH sensor
Salinity sensor
Water Temperature Sensor
theme
None, User defined
sample identification
treatment
temperature of sample or a generic measurement (not in-situ water temp)
irradiance
No BCO-DMO term
salinity
pH
featureType
BCO-DMO Standard Parameters
Light Meter
pH Sensor
Salinity Sensor
Water Temperature Sensor
instrument
BCO-DMO Standard Instruments
M77
service
Deployment Activity
Peruvian ocean margin
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
The Use of Nitrogen Isotopes of Amino Acids To Understand Marine Sedimentary 15N Records
https://osprey.bco-dmo.org/project/704684
The Use of Nitrogen Isotopes of Amino Acids To Understand Marine Sedimentary 15N Records
<p>The bioavailability of nutrients plays a crucial role in oceanic biological productivity, the carbon cycle, and climate change. The global ocean inventory of nitrogen (N) is determined by the balance of N-fixation (sources) and denitrification (sinks). In this three-year project, a researcher from the University of California, Santa Cruz, will focus on developing compound-specific N isotope (d15N) analysis of amino acids as a new tool for understanding N source and transformation of organic matter in paleo-reservoirs. The offsets in the isotopic ratios of individual amino acid groups may yield information about trophic transfer, heterotrophic microbial reworking, and autotrophic versus heterotrophic sources. By measuring and comparing the bulk and amino acid d15N in size-fractioned samples from plankton tows, sediments traps, and multi-cores in oxic and suboxic depositional environments, the researcher will: (1) Provide a proxy of the d15N of average exported photoautotrophic organic matter; and (2) Provide a new level of detail into sedimentary organic N degradation and preservation.</p>
<p>Broader impacts:<br />
This project will improve understanding of the fundamental underpinnings and behaviors of d15N amino acid patterns and how they behave in contrasting sedimentary environments, while also developing a potential paleoceanographic proxy. Funding will support a graduate student and undergraduate research at the institution. The researcher will also conduct community outreach in the form of a workshop/tutorial on the proxy development.</p>
Amino Acid Sediment 15N
largerWorkCitation
project
eng; USA
oceans
Peruvian ocean margin
2008-11-01
2008-12-31
California Margin , Santa Barbara Basin , CA current system, Eastern Tropical Pacific
0
BCO-DMO catalogue of parameters from Treatment description and growth parameters for Thalassiosira weissflogii generating d13C-AA, collected on R/V Meteor M77 in the Peruvian ocean margin from November to December 2008.
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/715867.rdf
Name: ID
Units: unitless
Description: <p>Treatment ID</p>
http://lod.bco-dmo.org/id/dataset-parameter/715868.rdf
Name: Treatment
Units: unitless
Description: <p>Treatment description</p>
http://lod.bco-dmo.org/id/dataset-parameter/715869.rdf
Name: Temp
Units: celsius
Description: <p>Temperature. Temperature values marked with (1) are means of outdoor conditions</p>
http://lod.bco-dmo.org/id/dataset-parameter/715870.rdf
Name: Light
Units: umol/square meter/second
Description: <p>Irradiance. Irradiance values marked with (2) are means of outdoor conditions.</p>
http://lod.bco-dmo.org/id/dataset-parameter/715871.rdf
Name: Light_cycle
Units: D/L (h)
Description: <p>Light cycle conditions</p>
http://lod.bco-dmo.org/id/dataset-parameter/715872.rdf
Name: Salinity
Units: PSU
Description: <p>Salinity</p>
http://lod.bco-dmo.org/id/dataset-parameter/715873.rdf
Name: pH_init
Units: pH
Description: <p>pH initial value; free scale at 17 degrees Celsius</p>
http://lod.bco-dmo.org/id/dataset-parameter/715874.rdf
Name: pH_term
Units: pH
Description: <p>pH term. Value; free scale at 17 degrees Celsius</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
671
https://darchive.mblwhoilibrary.org/bitstream/1912/23868/1/dataset-715856_algal-treatments__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.715856.1
download
onLine
dataset
<p><strong>Culturing</strong></p>
<p>The marine diatom Thalassiosira weissflogii Grunow (strain CCMP 1010) was cultured in sterile filtered natural North Sea water (Schleswig-Holstein, Germany) or Baltic Sea water (Schleswig-Holstein, Germany). The medium was enriched with f/4 concentrations of macro- and micronutrients (nitrate, phosphate, silicic acid, trace metal mixture, vitamin mixture (Guillard and Ryther, 1962). All experiments were performed in sterile 2.1 L Schott Duran glass bottles. These bottles were made of borosilicate glass (filters UV radiation &lt;310 nm) except for the quartz glass bottles (pure silica without UV radiation filter) used in the UV experiment. The cultures were either incubated in climate chambers with 400 –700 nm radiation or 10 cm below water level at low tide in Kiel Fjord in May 2011. Water temperature and light irradiance data were obtained from the weather station maintained by the GEOMAR institute in Kiel, Germany. Growth conditions for the various treatments, i.e. salinity, pH, temperature, and irradiance are given in Table 1. pH values (reported on free scale) were measured &nbsp;with separate glass and reference electrodes (Metrohm) and calculated with equation 3 from DOE 2007 chapter 6b (Dickson et al., 2007) as described in (Bach et al., 2012). Cultures were inoculated with densities of 20 cells ml-1. &nbsp;Cell densities and equivalent spherical diameters were determined with a Coulter Counter (Beckman Coulter) at the beginning and the end of the experiment, respectively. When incubations ended, cells were filtered on 47 mm diameter, 5 um mesh size Nucleopore Track-Etch Membrane filters (Whatman) and frozen at -18 deg C immediately after filtration.</p>
Specified by the Principal Investigator(s)
<p>Thermo-Finnigan Isodat software and Microsoft Excel 2013.</p>
<p><strong>BCO-DMO Processing:</strong></p>
<p>- changed column names to comply with BCO-DMO standards<br />
- captured superscript numbers by changing them to (1) and (2) where appropriate<br />
- changed degree symbol to "deg"<br />
- deleted blank columns<br />
- combined pH headers to create pH columns pH_init and pH_term</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Light meter
Light meter
PI Supplied Instrument Name: Light meter PI Supplied Instrument Description:Used to measure irradiance Instrument Name: Light Meter Instrument Short Name:Light Meter Instrument Description: Light meters are instruments that measure light intensity. Common units of measure for light intensity are umol/m2/s or uE/m2/s (micromoles per meter squared per second or microEinsteins per meter squared per second). (example: LI-COR 250A)
pH sensor
pH sensor
PI Supplied Instrument Name: pH sensor PI Supplied Instrument Description:Used to measure pH Instrument Name: pH Sensor Instrument Short Name:pH Sensor Instrument Description: An instrument that measures the hydrogen ion activity in solutions.
The overall concentration of hydrogen ions is inversely related to its pH. The pH scale ranges from 0 to 14 and indicates whether acidic (more H+) or basic (less H+).
Salinity sensor
Salinity sensor
PI Supplied Instrument Name: Salinity sensor PI Supplied Instrument Description:Used to measure salinity Instrument Name: Salinity Sensor Instrument Short Name:Salinity Sensor Instrument Description: Category of instrument that simultaneously measures electrical conductivity and temperature in the water column to provide temperature and salinity data. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/350/
Water Temperature Sensor
Water Temperature Sensor
PI Supplied Instrument Name: Water Temperature Sensor PI Supplied Instrument Description:Used to measure temperature Instrument Name: Water Temperature Sensor Instrument Short Name:Water Temp Sensor Instrument Description: General term for an instrument that measures the temperature of the water with which it is in contact (thermometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/134/
Cruise: M77
M77
Community Standard Description
International Council for the Exploration of the Sea
R/V Meteor
vessel
M77
Ralph Schneider
University of Konstanz
https://www.ldf.uni-hamburg.de/meteor/wochenberichte/wochenberichte-meteor/m77/m77-2-scr.pdf
Report describing M77
Community Standard Description
International Council for the Exploration of the Sea
R/V Meteor
vessel