http://lod.bco-dmo.org/id/dataset/715977
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2017-09-29
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Mean d15N of individual amino acids and bulk organic matter for five plankton size fractions
2017-09-14
publication
2017-09-14
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-03-19
publication
https://doi.org/10.1575/1912/bco-dmo.715977.1
Matthew D. McCarthy
University of California-Santa Cruz
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: McCarthy, M. (2017) Mean d15N of individual amino acids and bulk organic matter for five plankton size fractions. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2017-09-14 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.715977.1 [access date]
Mean d15N of individual amino acids and bulk organic matter for five plankton size fractions Dataset Description: <p>&nbsp;Mean d15N of individual amino acids and bulk organic matter for five plankton size fractions.</p> Methods and Sampling: <p><strong>Sampling</strong></p>
<p>Plankton samples were obtained during Leg 8 of the Malaspina-2010 expedition on R/V Sarmiento de Gamboa (January-March 2011), on a transect predominantly along 24ºN, between the Canary Island and Florida. Briefly, plankton samples were collected by vertical tows of a microplankton net (40 µm mesh size) and a mesoplankton net (200 µm mesh size) through the upper 200 m of the water column. Sampling was between 10:00 and 16:00 h GMT. Plankton was separated into five size fractions (40–200, 200–500, 500–1000, 1000–2000 and 2000 µm) by gentle filtration of the samples by a graded series of nylon sieves (2000, 1000, 500, 200 and 40 µm). Large gelatinous organisms were removed before filtration. Aliquots for each size fraction were collected on pre-weighed glass-fiber filters, dried (60ºC, 48 h) and stored in a desiccator before determination of biomass (dry weight), carbon and nitrogen content and natural abundance of stable carbon and nitrogen isotopes ashore. Nominal values of the individual size of organisms in each size fraction were estimated as the geometric mean of the values defining each size interval and expressed as carbon content (µg C) in a logarithmic scale</p>
<p><strong>Bulk δ15N analysis</strong></p>
<p>After determination of dry weight, finely ground aliquots of each size fraction were packed in tin capsules for elemental and stable isotope analysis by conversion into CO2 and N2 in an elemental analyzer (Carlo Erba CHNSO 1108) coupled to an isotope-ratio mass-spectrometer (Finnigan Mat Delta Plus).</p>
<p><strong>Compound-specific amino acid δ15N analysis</strong></p>
<p>Samples for CSI-AA were selected to span gradients in 15Nbulk values. We&nbsp;chose plankton from four sampling stations in each of the three zones (eastern, central and western regions).&nbsp; Individual samples were then pooled (quantitatively, so that each subsample was represented equally in the final composite) to have enough material in each size fraction for CSI-AA. In total 15 samples in the transect were chosen for CSI-AA. Approximately 1 mg of total dry plankton material was then hydrolyzed for subsequent analysis.</p>
<p>The δ15N values of individual AAs were measured via GC-IRMS, after 6 N HCl acid hydrolysis and the formation of TFA ester derivatives following previously published methods.&nbsp; Briefly, amino acids were liberated by hydrolysis (6 N HCl, 20 hr at 110uC) under nitrogen, and TFA derivatives subsequently prepared from free AA: isopropyl esters were made with a 1:5 mixture of Acetyl Chloride (AcCl):2-propanol (110uC, 60 minutes), and then acylated using a 1:3 mixture of Dichloromethane:Trifluroacetyl acetate (DCM:TFAA) (100uC, 15 minutes). Derivatized AAs were dissolved in DCM to a final ratio of approximately 2 mg of original dry sample to 250 ml DCM. After derivatization, samples were analyzed by a thermos Trace Ultra gas chromatograph coupled to a Finnegan Delta-Plus isotope ratio mass spectrometer (GC-IRMS). AAs were separated using a 50 m, 0.32 ID Hewlett Packard Ultra-1 column with 1 mm film thickness.&nbsp; AAs were measured based on n = 4 injections, and the average mean deviations for individual AA d15N measurements across all sample replicates was 0.5%.</p>
<p>Under these conditions, we determined δ15N values for 12 AAs: glutamic acid + glutamine (Glx), aspartic acid + asparagine (Asp), alanine (Ala), Isoleucine (Ile), Leucine (Leu), Proline (Pro), valine (Val), glycine (Gly), serine (Ser), Lysine (Lys), phenylalanine (Phe), and Threonine (Thr). Each AA was run four times on the GC-IRMS..&nbsp; AA values were categorized and presented in 3 groups, based on their relative 15N values changes with trophic transfer: the source AAs (Gly, Ser, Lys, Phe), the trophic AAs (Glx, Asp, Ala, Ile, Leu, Pro and Val), and one “metabolic” AA (Thr).</p>
<p><strong>Trophic position and ΣV&nbsp;</strong></p>
<p>To calculate CSI-AA based TP of plankton we used the most widely used current equation and TEF value, based on the isotopic offset between Glx and Phe:</p>
<p>TP&nbsp; = (δ15NGlx – δ15NPhe – 3.4)/7.6 +1</p>
<p>where δ15NGlx and δ15NPhe are measured values, +3.4‰ is the assumed isotopic difference between the Glx and Phe in primary producers, and +7.6‰ is the assumed 15N enrichment in Glx relative to Phe with each trophic transfer from food source to consumer (TEF value). The standard errors in the estimation of TP, computed by propagation of analytical error in the individual AA determinations, did not exceed 0.1 TP.</p>
<p>The δ15N value of total hydrolysable AAs (δ15NTHAA) is used as a proxy for total protein δ15N value, and was estimated as the molar-weighted average of individual δ15N values:</p>
<p>δ15NTHAA = Σ (δ15NAA * mol% AA)</p>
<p>where δ15NAA is the δ15N value of each individual AA measured and mol%AA is the molar percentage contribution of each AA. In our study we used the δ15N value of each individual AA and mol%AA were obtained from Lehman (2009).</p>
<p>The degradation index ΣV is a measure of the relative resynthesis of the original autotrophic AA pool in detritus or different organisms (plankton size fractions, in our case) was for each size individual fraction sample as the mean deviation of δ15N of individual trophic amino acid, from their average:</p>
<p>ΣV&nbsp; = Σ (AAi – Avg trp) / n</p>
<p>Where AAi were individual δ15N amino acid values, Avg trp is the average value and n the total number of trophic amino acids.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1131816 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1131816
completed
Matthew D. McCarthy
University of California-Santa Cruz
831-459-1533
1156 High Street
Santa Cruz
CA
95064
USA
mdmccar@ucsc.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Zone
plankton_size_fraction
Bulk_15N
Bulk_15N_significant_difference
trophicAA_Glx
trophicAA_Glx_significant_difference
trophicAA_Asp
trophicAA_Asp_significant_difference
trophicAA_Ala
trophicAA_Ala_significant_difference
trophicAA_Ile
trophicAA_Ile_significant_difference
trophicAA_Leu
trophicAA_Leu_significant_difference
trophicAA_Pro
trophicAA_Pro_significant_difference
trophicAA_Val
trophicAA_Val_significant_difference
sourceAA_Gly
sourceAA_Gly_significant_difference
sourceAA_Ser
sourceAA_Ser_significant_difference
sourceAA_Lys
sourceAA_Lys_significant_difference
sourceAA_Phe
sourceAA_Phe_significant_difference
metabAA_Thr
metabAA_Thr_significant_difference
CSI_AA_TP
CSI_AA_TP_significant_difference
CSI_AA_sigmaV
CSI_AA_sigmaV_significant_difference
PDZ Europa ANCA-GSL elemental analyzer
Thermo Trace GOLD GC
PDZ Europa 20-20 isotope ratio mass spectrometer
MAT 253 isotope ratio mass spectrometer (IRMS)
Light meter
pH sensor
Salinity sensor
Water Temperature Sensor
theme
None, User defined
site description
cell_size
d15N
amino acid concentration
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
Elemental Analyzer
Gas Chromatograph
Isotope-ratio Mass Spectrometer
Isotope-ratio Mass Spectrometer
Light Meter
pH Sensor
Salinity Sensor
Water Temperature Sensor
instrument
BCO-DMO Standard Instruments
Malaspina_2011
service
Deployment Activity
Subtropical North Atlantic Ocean
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
The Use of Nitrogen Isotopes of Amino Acids To Understand Marine Sedimentary 15N Records
https://osprey.bco-dmo.org/project/704684
The Use of Nitrogen Isotopes of Amino Acids To Understand Marine Sedimentary 15N Records
<p>The bioavailability of nutrients plays a crucial role in oceanic biological productivity, the carbon cycle, and climate change. The global ocean inventory of nitrogen (N) is determined by the balance of N-fixation (sources) and denitrification (sinks). In this three-year project, a researcher from the University of California, Santa Cruz, will focus on developing compound-specific N isotope (d15N) analysis of amino acids as a new tool for understanding N source and transformation of organic matter in paleo-reservoirs. The offsets in the isotopic ratios of individual amino acid groups may yield information about trophic transfer, heterotrophic microbial reworking, and autotrophic versus heterotrophic sources. By measuring and comparing the bulk and amino acid d15N in size-fractioned samples from plankton tows, sediments traps, and multi-cores in oxic and suboxic depositional environments, the researcher will: (1) Provide a proxy of the d15N of average exported photoautotrophic organic matter; and (2) Provide a new level of detail into sedimentary organic N degradation and preservation.</p>
<p>Broader impacts:<br />
This project will improve understanding of the fundamental underpinnings and behaviors of d15N amino acid patterns and how they behave in contrasting sedimentary environments, while also developing a potential paleoceanographic proxy. Funding will support a graduate student and undergraduate research at the institution. The researcher will also conduct community outreach in the form of a workshop/tutorial on the proxy development.</p>
Amino Acid Sediment 15N
largerWorkCitation
project
eng; USA
oceans
Subtropical North Atlantic Ocean
-24
-24
24
24
2011-01-01
2011-03-31
California Margin , Santa Barbara Basin , CA current system, Eastern Tropical Pacific
0
BCO-DMO catalogue of parameters from Mean d15N of individual amino acids and bulk organic matter for five plankton size fractions
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/715992.rdf
Name: Zone
Units: unitless
Description: <p>Location where plankton size fractions were analyzed; The West Central or Eastern Zone.</p>
http://lod.bco-dmo.org/id/dataset-parameter/715993.rdf
Name: plankton_size_fraction
Units: microns
Description: <p>Plankton size fraction range</p>
http://lod.bco-dmo.org/id/dataset-parameter/715994.rdf
Name: Bulk_15N
Units: per mil
Description: <p>15N/14N isotopic ratio total sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/715995.rdf
Name: Bulk_15N_significant_difference
Units: per mil
Description: <p>Significant difference; The significant differences between fractions for each zone are indicated with differenent letters (a b and c); Cases with no letters indicate no significant differences within the zones sampled.</p>
http://lod.bco-dmo.org/id/dataset-parameter/715996.rdf
Name: trophicAA_Glx
Units: per mil
Description: <p>Gln+Glu d15N value</p>
http://lod.bco-dmo.org/id/dataset-parameter/715997.rdf
Name: trophicAA_Glx_significant_difference
Units: per mil
Description: <p>Significant difference; The significant differences between fractions for each zone are indicated with differenent letters (a b and c); Cases with no letters indicate no significant differences within the zones sampled.</p>
http://lod.bco-dmo.org/id/dataset-parameter/715998.rdf
Name: trophicAA_Asp
Units: per mil
Description: <p>Aspartic Acid + Asparagine d15N value</p>
http://lod.bco-dmo.org/id/dataset-parameter/715999.rdf
Name: trophicAA_Asp_significant_difference
Units: per mil
Description: <p>Significant difference; The significant differences between fractions for each zone are indicated with differenent letters (a b and c); Cases with no letters indicate no significant differences within the zones sampled.</p>
http://lod.bco-dmo.org/id/dataset-parameter/716000.rdf
Name: trophicAA_Ala
Units: per mil
Description: <p>Alanine d15N value</p>
http://lod.bco-dmo.org/id/dataset-parameter/716001.rdf
Name: trophicAA_Ala_significant_difference
Units: per mil
Description: <p>Significant difference; The significant differences between fractions for each zone are indicated with differenent letters (a b and c); Cases with no letters indicate no significant differences within the zones sampled.</p>
http://lod.bco-dmo.org/id/dataset-parameter/716002.rdf
Name: trophicAA_Ile
Units: per mil
Description: <p>Isoleucine d15N value</p>
http://lod.bco-dmo.org/id/dataset-parameter/716003.rdf
Name: trophicAA_Ile_significant_difference
Units: per mil
Description: <p>Significant difference; The significant differences between fractions for each zone are indicated with differenent letters (a b and c); Cases with no letters indicate no significant differences within the zones sampled.</p>
http://lod.bco-dmo.org/id/dataset-parameter/716004.rdf
Name: trophicAA_Leu
Units: per mil
Description: <p>Leucine d15N value</p>
http://lod.bco-dmo.org/id/dataset-parameter/716005.rdf
Name: trophicAA_Leu_significant_difference
Units: per mil
Description: <p>Significant difference; The significant differences between fractions for each zone are indicated with differenent letters (a b and c); Cases with no letters indicate no significant differences within the zones sampled.</p>
http://lod.bco-dmo.org/id/dataset-parameter/716006.rdf
Name: trophicAA_Pro
Units: per mil
Description: <p>Proline d15N value</p>
http://lod.bco-dmo.org/id/dataset-parameter/716007.rdf
Name: trophicAA_Pro_significant_difference
Units: per mil
Description: <p>Significant difference; The significant differences between fractions for each zone are indicated with differenent letters (a b and c); Cases with no letters indicate no significant differences within the zones sampled.</p>
http://lod.bco-dmo.org/id/dataset-parameter/716008.rdf
Name: trophicAA_Val
Units: per mil
Description: <p>Valine d15N value</p>
http://lod.bco-dmo.org/id/dataset-parameter/716009.rdf
Name: trophicAA_Val_significant_difference
Units: per mil
Description: <p>Significant difference; The significant differences between fractions for each zone are indicated with differenent letters (a b and c); Cases with no letters indicate no significant differences within the zones sampled.</p>
http://lod.bco-dmo.org/id/dataset-parameter/716010.rdf
Name: sourceAA_Gly
Units: per mil
Description: <p>Glycine d15N value</p>
http://lod.bco-dmo.org/id/dataset-parameter/716011.rdf
Name: sourceAA_Gly_significant_difference
Units: per mil
Description: <p>Significant difference; The significant differences between fractions for each zone are indicated with differenent letters (a b and c); Cases with no letters indicate no significant differences within the zones sampled.</p>
http://lod.bco-dmo.org/id/dataset-parameter/716012.rdf
Name: sourceAA_Ser
Units: per mil
Description: <p>Serine d15N value</p>
http://lod.bco-dmo.org/id/dataset-parameter/716013.rdf
Name: sourceAA_Ser_significant_difference
Units: per mil
Description: <p>Significant difference; The significant differences between fractions for each zone are indicated with differenent letters (a b and c); Cases with no letters indicate no significant differences within the zones sampled.</p>
http://lod.bco-dmo.org/id/dataset-parameter/716014.rdf
Name: sourceAA_Lys
Units: per mil
Description: <p>Lysine d15N value</p>
http://lod.bco-dmo.org/id/dataset-parameter/716015.rdf
Name: sourceAA_Lys_significant_difference
Units: per mil
Description: <p>Significant difference; The significant differences between fractions for each zone are indicated with differenent letters (a b and c); Cases with no letters indicate no significant differences within the zones sampled.</p>
http://lod.bco-dmo.org/id/dataset-parameter/716016.rdf
Name: sourceAA_Phe
Units: per mil
Description: <p>Phenylalanine d15N value</p>
http://lod.bco-dmo.org/id/dataset-parameter/716017.rdf
Name: sourceAA_Phe_significant_difference
Units: per mil
Description: <p>Significant difference; The significant differences between fractions for each zone are indicated with differenent letters (a b and c); Cases with no letters indicate no significant differences within the zones sampled.</p>
http://lod.bco-dmo.org/id/dataset-parameter/716018.rdf
Name: metabAA_Thr
Units: per mil
Description: <p>Threonine d15N value</p>
http://lod.bco-dmo.org/id/dataset-parameter/716019.rdf
Name: metabAA_Thr_significant_difference
Units: per mil
Description: <p>Significant difference; The significant differences between fractions for each zone are indicated with differenent letters (a b and c); Cases with no letters indicate no significant differences within the zones sampled.</p>
http://lod.bco-dmo.org/id/dataset-parameter/716020.rdf
Name: CSI_AA_TP
Units: unitless
Description: <p>Trophic Position</p>
http://lod.bco-dmo.org/id/dataset-parameter/716021.rdf
Name: CSI_AA_TP_significant_difference
Units: unitless
Description: <p>Significant difference; The significant differences between fractions for each zone are indicated with differenent letters (a b and c); Cases with no letters indicate no significant differences within the zones sampled.</p>
http://lod.bco-dmo.org/id/dataset-parameter/716022.rdf
Name: CSI_AA_sigmaV
Units: unitless
Description: <p>CSIAA Degradation Parameter</p>
http://lod.bco-dmo.org/id/dataset-parameter/716023.rdf
Name: CSI_AA_sigmaV_significant_difference
Units: unitless
Description: <p>Significant difference; The significant differences between fractions for each zone are indicated with differenent letters (a b and c); Cases with no letters indicate no significant differences within the zones sampled.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
3984
https://darchive.mblwhoilibrary.org/bitstream/1912/23864/1/dataset-715977_atlantic-zooplankton-aa-15n-values__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.715977.1
download
onLine
dataset
<p><strong>Sampling</strong></p>
<p>Plankton samples were obtained during Leg 8 of the Malaspina-2010 expedition on R/V Sarmiento de Gamboa (January-March 2011), on a transect predominantly along 24ºN, between the Canary Island and Florida. Briefly, plankton samples were collected by vertical tows of a microplankton net (40 µm mesh size) and a mesoplankton net (200 µm mesh size) through the upper 200 m of the water column. Sampling was between 10:00 and 16:00 h GMT. Plankton was separated into five size fractions (40–200, 200–500, 500–1000, 1000–2000 and 2000 µm) by gentle filtration of the samples by a graded series of nylon sieves (2000, 1000, 500, 200 and 40 µm). Large gelatinous organisms were removed before filtration. Aliquots for each size fraction were collected on pre-weighed glass-fiber filters, dried (60ºC, 48 h) and stored in a desiccator before determination of biomass (dry weight), carbon and nitrogen content and natural abundance of stable carbon and nitrogen isotopes ashore. Nominal values of the individual size of organisms in each size fraction were estimated as the geometric mean of the values defining each size interval and expressed as carbon content (µg C) in a logarithmic scale</p>
<p><strong>Bulk δ15N analysis</strong></p>
<p>After determination of dry weight, finely ground aliquots of each size fraction were packed in tin capsules for elemental and stable isotope analysis by conversion into CO2 and N2 in an elemental analyzer (Carlo Erba CHNSO 1108) coupled to an isotope-ratio mass-spectrometer (Finnigan Mat Delta Plus).</p>
<p><strong>Compound-specific amino acid δ15N analysis</strong></p>
<p>Samples for CSI-AA were selected to span gradients in 15Nbulk values. We&nbsp;chose plankton from four sampling stations in each of the three zones (eastern, central and western regions).&nbsp; Individual samples were then pooled (quantitatively, so that each subsample was represented equally in the final composite) to have enough material in each size fraction for CSI-AA. In total 15 samples in the transect were chosen for CSI-AA. Approximately 1 mg of total dry plankton material was then hydrolyzed for subsequent analysis.</p>
<p>The δ15N values of individual AAs were measured via GC-IRMS, after 6 N HCl acid hydrolysis and the formation of TFA ester derivatives following previously published methods.&nbsp; Briefly, amino acids were liberated by hydrolysis (6 N HCl, 20 hr at 110uC) under nitrogen, and TFA derivatives subsequently prepared from free AA: isopropyl esters were made with a 1:5 mixture of Acetyl Chloride (AcCl):2-propanol (110uC, 60 minutes), and then acylated using a 1:3 mixture of Dichloromethane:Trifluroacetyl acetate (DCM:TFAA) (100uC, 15 minutes). Derivatized AAs were dissolved in DCM to a final ratio of approximately 2 mg of original dry sample to 250 ml DCM. After derivatization, samples were analyzed by a thermos Trace Ultra gas chromatograph coupled to a Finnegan Delta-Plus isotope ratio mass spectrometer (GC-IRMS). AAs were separated using a 50 m, 0.32 ID Hewlett Packard Ultra-1 column with 1 mm film thickness.&nbsp; AAs were measured based on n = 4 injections, and the average mean deviations for individual AA d15N measurements across all sample replicates was 0.5%.</p>
<p>Under these conditions, we determined δ15N values for 12 AAs: glutamic acid + glutamine (Glx), aspartic acid + asparagine (Asp), alanine (Ala), Isoleucine (Ile), Leucine (Leu), Proline (Pro), valine (Val), glycine (Gly), serine (Ser), Lysine (Lys), phenylalanine (Phe), and Threonine (Thr). Each AA was run four times on the GC-IRMS..&nbsp; AA values were categorized and presented in 3 groups, based on their relative 15N values changes with trophic transfer: the source AAs (Gly, Ser, Lys, Phe), the trophic AAs (Glx, Asp, Ala, Ile, Leu, Pro and Val), and one “metabolic” AA (Thr).</p>
<p><strong>Trophic position and ΣV&nbsp;</strong></p>
<p>To calculate CSI-AA based TP of plankton we used the most widely used current equation and TEF value, based on the isotopic offset between Glx and Phe:</p>
<p>TP&nbsp; = (δ15NGlx – δ15NPhe – 3.4)/7.6 +1</p>
<p>where δ15NGlx and δ15NPhe are measured values, +3.4‰ is the assumed isotopic difference between the Glx and Phe in primary producers, and +7.6‰ is the assumed 15N enrichment in Glx relative to Phe with each trophic transfer from food source to consumer (TEF value). The standard errors in the estimation of TP, computed by propagation of analytical error in the individual AA determinations, did not exceed 0.1 TP.</p>
<p>The δ15N value of total hydrolysable AAs (δ15NTHAA) is used as a proxy for total protein δ15N value, and was estimated as the molar-weighted average of individual δ15N values:</p>
<p>δ15NTHAA = Σ (δ15NAA * mol% AA)</p>
<p>where δ15NAA is the δ15N value of each individual AA measured and mol%AA is the molar percentage contribution of each AA. In our study we used the δ15N value of each individual AA and mol%AA were obtained from Lehman (2009).</p>
<p>The degradation index ΣV is a measure of the relative resynthesis of the original autotrophic AA pool in detritus or different organisms (plankton size fractions, in our case) was for each size individual fraction sample as the mean deviation of δ15N of individual trophic amino acid, from their average:</p>
<p>ΣV&nbsp; = Σ (AAi – Avg trp) / n</p>
<p>Where AAi were individual δ15N amino acid values, Avg trp is the average value and n the total number of trophic amino acids.</p>
Specified by the Principal Investigator(s)
<p>Thermo-Finnigan Isodat software and Microsoft Excel 2013.</p>
<p><strong>BCO-DMO Processing:</strong><br />
- changed column names to comply with BCO-DMO standards<br />
- added columns for significant differences to capture the superscript letters that accompanied some data point. All added columns are named after the amino acid with "_significant_difference".<br />
- combined some headers to capture the metadata used to describe several columns (example trophic_AA_Glx)<br />
- changed &gt; to greater than<br />
- changed Σ to sigma</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PDZ Europa ANCA-GSL elemental analyzer
PDZ Europa ANCA-GSL elemental analyzer
PI Supplied Instrument Name: PDZ Europa ANCA-GSL elemental analyzer PI Supplied Instrument Description:Interfaced to a PDZ Europa 20-20 isotope ratio mass spectrometer (Sercon Ltd., Cheshire, UK) Instrument Name: Elemental Analyzer Instrument Short Name: Instrument Description: Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/
Thermo Trace GOLD GC
Thermo Trace GOLD GC
PI Supplied Instrument Name: Thermo Trace GOLD GC PI Supplied Instrument Description:Used with MAT 253 isotope ratio mass spectrometer (IRMS) via a GC-III combustion (C) interface (Thermo-Finnigan Corporation) Instrument Name: Gas Chromatograph Instrument Short Name:Gas Chromatograph Instrument Description: Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB02/
PDZ Europa 20-20 isotope ratio mass spectrometer
PDZ Europa 20-20 isotope ratio mass spectrometer
PI Supplied Instrument Name: PDZ Europa 20-20 isotope ratio mass spectrometer PI Supplied Instrument Description:Used with PDZ Europa ANCA-GSL elemental analyzer Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
MAT 253 isotope ratio mass spectrometer (IRMS)
MAT 253 isotope ratio mass spectrometer (IRMS)
PI Supplied Instrument Name: MAT 253 isotope ratio mass spectrometer (IRMS) PI Supplied Instrument Description:Used with Thermo Trace GOLD GC Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Light meter
Light meter
PI Supplied Instrument Name: Light meter PI Supplied Instrument Description:Used to measure irradiance Instrument Name: Light Meter Instrument Short Name:Light Meter Instrument Description: Light meters are instruments that measure light intensity. Common units of measure for light intensity are umol/m2/s or uE/m2/s (micromoles per meter squared per second or microEinsteins per meter squared per second). (example: LI-COR 250A)
pH sensor
pH sensor
PI Supplied Instrument Name: pH sensor PI Supplied Instrument Description:Used to measure pH Instrument Name: pH Sensor Instrument Short Name:pH Sensor Instrument Description: An instrument that measures the hydrogen ion activity in solutions.
The overall concentration of hydrogen ions is inversely related to its pH. The pH scale ranges from 0 to 14 and indicates whether acidic (more H+) or basic (less H+).
Salinity sensor
Salinity sensor
PI Supplied Instrument Name: Salinity sensor PI Supplied Instrument Description:Used to measure salinity Instrument Name: Salinity Sensor Instrument Short Name:Salinity Sensor Instrument Description: Category of instrument that simultaneously measures electrical conductivity and temperature in the water column to provide temperature and salinity data. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/350/
Water Temperature Sensor
Water Temperature Sensor
PI Supplied Instrument Name: Water Temperature Sensor PI Supplied Instrument Description:Used to measure temperature Instrument Name: Water Temperature Sensor Instrument Short Name:Water Temp Sensor Instrument Description: General term for an instrument that measures the temperature of the water with which it is in contact (thermometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/134/
Cruise: Malaspina_2011
Malaspina_2011
R/V Sarmiento de Gamboa
vessel
Malaspina_2011
Pia Moisander
University of Massachusetts Dartmouth SMAST
R/V Sarmiento de Gamboa
vessel