http://lod.bco-dmo.org/id/dataset/716316
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2017-10-05
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Sea star catch data and chlorophyll a collected during benthic dredge operations on board the R/V Clifford A. Barnes cruise CB1038 from Jan 5 to 10th in 2016 between Seattle and Bellingham, WA (Sea Star Microbiology project)
2017-10-05
publication
2017-10-05
revision
BCO-DMO Linked Data URI
2017-10-05
creation
http://lod.bco-dmo.org/id/dataset/716316
Ian Hewson
Cornell University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Hewson, I. (2017) Sea star catch data and chlorophyll a collected during benthic dredge operations on board the R/V Clifford A. Barnes cruise CB1038 from Jan 5 to 10th in 2016 between Seattle and Bellingham, WA (Sea Star Microbiology project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2017-10-05 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/716316 [access date]
Sea star catch data and chlorophyll a Dataset Description: <p>This dataset includes sea star catch data by species collected during benthic dredge operations on board the R/V Clifford Barnes cruise CB1038, 5 – 10 January 2016 between Seattle and Bellingham, WA. Chlorophyll a concentrations are also included.</p>
<p>The purpose of this survey was to determine the distribution of Sea Star Wasting Disease (SSWD) in sea star populations inhabiting the 30 – 50m depth range at locations around the Salish Sea in Winter 2015-2016.&nbsp;</p> Methods and Sampling: <p><strong>Methodology:</strong></p>
<p>A survey of SSWD was performed in Salish Sea waters 30 – 50m to determine the presence of Sea Star Wasting Disease (SSWD). Asteroids were frozen for later determination of viral prevalence and load of the sea star associated densovirus (SSaDV).&nbsp; Chlorophyll a concentrations as measured by acetone extracted fluorometry.</p>
<p><strong>Sampling and analytical procedures:&nbsp;</strong></p>
<p>Benthic dredge operations were performed on board the R/V Clifford A. Barnes during a 5-day cruise from Seattle to Bellingham, encompassing the range of the early (2013-2014) range of SSWD. Dredge operations comprised a 10-minute dredge following the 30 – 50m contour (determined using depth sounder). Dredge materials were emptied on deck, rinsed with flow-through seawater, and then quantified by species of sea star and presence or absence of SSWD. In addition, sympatric echinoderms (holothurians and echinoids) were also quantified. At each station, samples for chlorophyll a (2L surface seawater), virioplankton composition (20L), and sediments (5ml via Van Veen grab) were also taken. In addition, a 5-minute plankton tow (202um 1m net) was performed. After retrieval, asteroids were photographed to document overall size, and if &gt;5 cm arm diameter dissected into constituent tissues (pyloric caeca, gonad, coelomic fluid and body wall). Animals that were &lt; 5cm were frozen as whole animals.&nbsp;</p>
<p>Chlorophyll a was determined using acetone-extracted fluorometry. Seawater (1L) was filtered over a 25mm diameter GF/A filter, which was then folded and placed into light-impermeable foil, and frozen prior to analysis. The filter was then extracted using 90% acetone (5ml) in 100x13mm borosilicate glass tubes overnight at -20oC. The extract was then quantified using a Turner Designs Aquafluor fluorometer.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1537111 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1537111
completed
Ian Hewson
Cornell University
1-607-255-0151
Department of Microbiology Wing Hall 403
Ithaca
NY
14853
USA
hewson@cornell.edu
pointOfContact
asNeeded
Unknown
Station
Name
Month
Year
Lat_Decimal_Degrees
Long_Decimal_Degrees
Chlorophyll_a
Chlorophyll_a_SE
Crossaster_papposus
Dermasterias_imbricata
Evasterias_troschelii
Henricia_spp
Hippasteria_phrygiana
Luidia_foliolata
Mediaster_aequalis
Orthasterias_koehleri
Pisaster_brevispinus
Pteraster_tesselatus
Pycnopodia_helianthoides
Solaster_stimpsoni
Benthic dredge, Turner Designs Aquafluor
theme
None, User defined
station
site
month of year
year
latitude
longitude
chlorophyll a
count
featureType
BCO-DMO Standard Parameters
Benthic sampler
instrument
BCO-DMO Standard Instruments
CB1038
service
Deployment Activity
Seattle, Washington
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Microbial ecology of sea star wasting disease
http://seastarwastingdisease.wordpress.com
Microbial ecology of sea star wasting disease
<p>Beginning in June 2013 and continuing to present (May 2015), over 20 species of sea stars (Asteroidea, Echinodermata) have been affected by sea star wasting disease (SSWD), affecting populations from central Alaska to Baja California. The disease has lead to greatly reduced abundance or disappearance of these keystone predators, which may result in profound alteration to benthic community structure. Recent work has identified the sea star associated densovirus (SSaDV) as the most likely causative agent of the disease. SSaDV is related to densoviruses inhabiting other echinoderms worldwide, and has been present in West Coast asteroid populations for at least 72 years. Hence, there remain significant knowledge gaps in our understanding of how SSaDV actually elicits SSWD symptoms, especially how the echinoderm host, densovirus and microbiome constituents interact. This project will address three major questions: 1) does viral infection change the composition of the sea star microbiome?, 2) what is the variation of viral genomes and their associated virulence?, and 3) does larval dispersal spread the disease between habitats? This project will address these hypotheses through time-course measurements of host, pathogen and associated microorganisms, genome-genome comparisons between historical and contemporary viral strains, and through experiments targeting larvae and juvenile asteroids in aquaria and in nature. </p>
<p>This project will address three fundamental questions relating to Sea Star Wasting Disease (SSWD): 1) How does SSaDV causes SSWD symptoms and how does the disease progress from primary infection through animal mortality; 2) How do current genotypes of SSaDV vary from those present historically, and is virulence related to genome polymorphisms; and 3) Are larvae and juvenile asteroids differentially affected by SSaDV, and are broadcast-spawned bipinarria a viable mechanism for SSaDV dispersal between distant habitats. The first question will be addressed by experimental inoculation of naïve sea stars with SSaDV, then time-course monitoring of host transcription (i.e. transcriptomics via RNAseq), microbiome composition via 16S rRNA sequencing and quantitative PCR, and viral load and prevalence using quantitatice PCR. The second question will be addressed by amplifying the entire genome of SSaDV and related densoviruses, then perform genome-genome comparisons to identify polymorphic DNA in key protein-encoding regions. The third question will be addressed by collecting bipinarria from plankton at field locations adjacent to spawning asteroid populations, and by performing time-course observations of captive juvenile sea stars and monitoring their bacterial and viral loads using quantitative PCR. This work will be performed primarily in the Salish Sea region, with SSaDV - naïve asteroids collected from Alaskan waters.</p>
Sea Star Microbiology
largerWorkCitation
project
eng; USA
biota
oceans
Seattle, Washington
-123.1
-122.3236
47.575
48.7208
2017-10-05
Salish Sea and Alaskan Waters
0
BCO-DMO catalogue of parameters from Sea star catch data and chlorophyll a collected during benthic dredge operations on board the R/V Clifford A. Barnes cruise CB1038 from Jan 5 to 10th in 2016 between Seattle and Bellingham, WA (Sea Star Microbiology project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/719885.rdf
Name: Station
Units: unitless
Description: <p>Station number</p>
http://lod.bco-dmo.org/id/dataset-parameter/719886.rdf
Name: Name
Units: unitless
Description: <p>Location name</p>
http://lod.bco-dmo.org/id/dataset-parameter/719887.rdf
Name: Month
Units: unitless
Description: <p>Month of sampling in format mm</p>
http://lod.bco-dmo.org/id/dataset-parameter/719888.rdf
Name: Year
Units: unitless
Description: <p>Year of sampling in format yyyy</p>
http://lod.bco-dmo.org/id/dataset-parameter/719889.rdf
Name: Lat_Decimal_Degrees
Units: decimal degrees
Description: <p>Latitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/719890.rdf
Name: Long_Decimal_Degrees
Units: decimal degrees
Description: <p>Longitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/719891.rdf
Name: Chlorophyll_a
Units: micrograms per liter (ug/L)
Description: <p>Chlorophyll a concentration (acetone extracted)</p>
http://lod.bco-dmo.org/id/dataset-parameter/719892.rdf
Name: Chlorophyll_a_SE
Units: micrograms per liter (ug/L)
Description: <p>Chlorophyll a standard error</p>
http://lod.bco-dmo.org/id/dataset-parameter/719893.rdf
Name: Crossaster_papposus
Units: individuals
Description: <p>Number of Crossaster papposus caught</p>
http://lod.bco-dmo.org/id/dataset-parameter/719894.rdf
Name: Dermasterias_imbricata
Units: individuals
Description: <p>Number of Dermasterias imbricata caught</p>
http://lod.bco-dmo.org/id/dataset-parameter/719895.rdf
Name: Evasterias_troschelii
Units: individuals
Description: <p>Number of Evasterias troschelii caught</p>
http://lod.bco-dmo.org/id/dataset-parameter/719896.rdf
Name: Henricia_spp
Units: individuals
Description: <p>Number of Henricia spp caught</p>
http://lod.bco-dmo.org/id/dataset-parameter/719897.rdf
Name: Hippasteria_phrygiana
Units: individuals
Description: <p>Number of Hippasteria phrygiana caught</p>
http://lod.bco-dmo.org/id/dataset-parameter/719898.rdf
Name: Luidia_foliolata
Units: individuals
Description: <p>Number of Luidia foliolata caught</p>
http://lod.bco-dmo.org/id/dataset-parameter/719899.rdf
Name: Mediaster_aequalis
Units: individuals
Description: <p>Number of Mediaster aequalis caught</p>
http://lod.bco-dmo.org/id/dataset-parameter/719900.rdf
Name: Orthasterias_koehleri
Units: individuals
Description: <p>Number of Orthasterias koehleri caught</p>
http://lod.bco-dmo.org/id/dataset-parameter/719901.rdf
Name: Pisaster_brevispinus
Units: individuals
Description: <p>Number of Pisaster brevispinus caught</p>
http://lod.bco-dmo.org/id/dataset-parameter/719902.rdf
Name: Pteraster_tesselatus
Units: individuals
Description: <p>Number of Pteraster tesselatus caught</p>
http://lod.bco-dmo.org/id/dataset-parameter/719903.rdf
Name: Pycnopodia_helianthoides
Units: individuals
Description: <p>Number of Pycnopodia helianthoides caught</p>
http://lod.bco-dmo.org/id/dataset-parameter/719904.rdf
Name: Solaster_stimpsoni
Units: individuals
Description: <p>Number of Solaster stimpsoni caught</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2252
https://datadocs.bco-dmo.org/file/933jo1GizAzoj7/CAB.csv
CAB.csv
Primary data file for dataset ID 716316
download
https://osprey.bco-dmo.org/dataset/716316/data/download
download
onLine
dataset
<p><strong>Methodology:</strong></p>
<p>A survey of SSWD was performed in Salish Sea waters 30 – 50m to determine the presence of Sea Star Wasting Disease (SSWD). Asteroids were frozen for later determination of viral prevalence and load of the sea star associated densovirus (SSaDV).&nbsp; Chlorophyll a concentrations as measured by acetone extracted fluorometry.</p>
<p><strong>Sampling and analytical procedures:&nbsp;</strong></p>
<p>Benthic dredge operations were performed on board the R/V Clifford A. Barnes during a 5-day cruise from Seattle to Bellingham, encompassing the range of the early (2013-2014) range of SSWD. Dredge operations comprised a 10-minute dredge following the 30 – 50m contour (determined using depth sounder). Dredge materials were emptied on deck, rinsed with flow-through seawater, and then quantified by species of sea star and presence or absence of SSWD. In addition, sympatric echinoderms (holothurians and echinoids) were also quantified. At each station, samples for chlorophyll a (2L surface seawater), virioplankton composition (20L), and sediments (5ml via Van Veen grab) were also taken. In addition, a 5-minute plankton tow (202um 1m net) was performed. After retrieval, asteroids were photographed to document overall size, and if &gt;5 cm arm diameter dissected into constituent tissues (pyloric caeca, gonad, coelomic fluid and body wall). Animals that were &lt; 5cm were frozen as whole animals.&nbsp;</p>
<p>Chlorophyll a was determined using acetone-extracted fluorometry. Seawater (1L) was filtered over a 25mm diameter GF/A filter, which was then folded and placed into light-impermeable foil, and frozen prior to analysis. The filter was then extracted using 90% acetone (5ml) in 100x13mm borosilicate glass tubes overnight at -20oC. The extract was then quantified using a Turner Designs Aquafluor fluorometer.</p>
Specified by the Principal Investigator(s)
<p>CO-DMO Data Manager Processing Notes:<br />
* added a conventional header with dataset name, PI name, version date<br />
* modified parameter names to conform with BCO-DMO naming conventions<br />
* param name edits [space -&gt; _] [ period -&gt; deleted] [SE -&gt; Chlorophyll_a_SE]<br />
* made longitude decimal degrees negative<br />
* added month and year of sampling<br />
* species names edit to match the accepted species names using World Register of Marine Species (as of 2017-10-17).&nbsp; Names verified by data contributor.&nbsp; List of changes below.</p>
<p>Species names edited in dataset:<br />
Solaster stimpsonii to accepted name Solaster stimpsoni&nbsp; &nbsp;&nbsp;<a href="http://www.marinespecies.org/aphia.php?p=taxdetails&amp;id=292729" target="_blank">292729</a><br />
Evasterias troscheli&nbsp;to&nbsp;accepted name Evasterias troschelii&nbsp; &nbsp;<a href="http://www.marinespecies.org/aphia.php?p=taxdetails&amp;id=255040" target="_blank">255040</a><br />
Orthasterias kohleri&nbsp;to accepted name&nbsp;Orthasterias koehleri&nbsp; &nbsp;<a href="http://www.marinespecies.org/aphia.php?p=taxdetails&amp;id=255048" target="_blank">255048</a>Hippasteria<br />
spinosa&nbsp;<a href="http://www.marinespecies.org/aphia.php?p=taxdetails&amp;id=254900" target="_blank">254900</a>&nbsp;to&nbsp; accepted name Hippasteria phrygiana&nbsp; &nbsp;<a href="http://www.marinespecies.org/aphia.php?p=taxdetails&amp;id=124043" target="_blank">124043</a></p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Benthic dredge, Turner Designs Aquafluor
Benthic dredge, Turner Designs Aquafluor
PI Supplied Instrument Name: Benthic dredge, Turner Designs Aquafluor Instrument Name: Benthic sampler Instrument Short Name:Benthic sampler Instrument Description: A mechanical device that collects organisms from the seafloor. Includes dredges, sledges, weighted nets like beam trawls, and ROV manipulators.
A description of benthic samplers and links to further resources can be found at marinespecies.org:
http://marinespecies.org/introduced/wiki/Sampling_tools_for_the_marine_environment
Cruise: CB1038
CB1038
R/V Clifford A. Barnes
Community Standard Description
International Council for the Exploration of the Sea
R/V Clifford A. Barnes
vessel
R/V Clifford A. Barnes
Community Standard Description
International Council for the Exploration of the Sea
R/V Clifford A. Barnes
vessel