http://lod.bco-dmo.org/id/dataset/716455
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2017-10-09
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Fatty acid data from infauna in sediment cores at McMurdo Station, Antarctica in September, November and February of 2012 and 2013 (McMurdo Benthos project)
2017-10-11
publication
2017-10-11
revision
BCO-DMO Linked Data URI
2017-10-11
creation
http://lod.bco-dmo.org/id/dataset/716455
Andrew Thurber
Oregon State University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Thurber, A. (2017) Fatty acid data from infauna in sediment cores at McMurdo Station, Antarctica in September, November and February of 2012 and 2013 (McMurdo Benthos project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2017-10-11 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/716455 [access date]
Fatty acid composition of McMurdo Benthos Dataset Description: <p>Fatty acid data from infauna&nbsp;in sediment cores collected at the “Jetty” dive site, Hutt Point, McMurdo Station, Antarctica.&nbsp; Seasonal sampling took place in September, November and February 2012-2013.</p> Methods and Sampling: <p><strong>Methodology:&nbsp;</strong><br />
Sample collection and field treatment: Sediment cores were collected at the “Jetty” dive site location in September, November and February 2012-2013.&nbsp; These were sieved on a 300-micron sieve to collect macrofauna and members of the abundant species Spiophanes tcherniai (a spionid polychaete) and Edwarsia spp.&nbsp; (a sand anemone) were picked under a dissecting microscope and placed in filtered seawater overnight to allow gut evacuation. At this point, individuals were frozen separately at -80 C prior to lipid extraction.</p>
<p><strong>Sampling and analytical procedures:</strong><br />
Samples were freeze-dried and their lipids extracted using one-step extraction-transesterification procedure of Lewis et al. (2000) as used in Thurber (2014). In brief, individuals were placed in pre-cleaned and extracted sample vials, heated to 90 deg. C for one hour in a solution of methanol, chloroform, and hydrochloric acid (10:1:1 by volume) before cooling and water added to break the phase.&nbsp; Fatty acid methyl esters (FAMEs) were extracted in sequential addition of hexane:chloroform (4:1) and water further removed through the addition of sodium sulfate.&nbsp; The FAMEs were then ready for quantification on a GC-FID.&nbsp;</p>
<p>Isotopic analysis was measured on a Thermo 1310 gas chromatograph with a Flame Ionization detector using a TR-WaxMS column (30mx 0.32mm x 0.25 um).&nbsp; Peak identification was by comparison to known standards (for example the Supulco 32 FAME mixture).</p>
Funding provided by NSF Office of Polar Programs (formerly NSF PLR) (NSF OPP) Award Number: OPP-1103428 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1103428
onGoing
Andrew Thurber
Oregon State University
541-737-4500
athurber@ucsb.edu
pointOfContact
asNeeded
Unknown
Taxa
Sample_Identifier
Sample_date
Core_Replicate
Fatty_Acid_ID
Fatty_Acid_Concentration
lat
lon
Thermo 1310 gas chromatograph
theme
None, User defined
No BCO-DMO term
sample identification
date
core id
lipids in biota
latitude
longitude
featureType
BCO-DMO Standard Parameters
Gas Chromatograph
instrument
BCO-DMO Standard Instruments
Thurber_Hutt_Point_2012
service
Deployment Activity
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Microbe - Metazoan Interactions in an Antarctic Infaunal Community
https://osprey.bco-dmo.org/project/716214
Microbe - Metazoan Interactions in an Antarctic Infaunal Community
<p>The biota of the world's seafloor is fueled by bursts of seasonal primary production. For food-limited sediment communities to persist, a balance must exist between metazoan consumption of and competition with bacteria, a balance which likely changes through the seasons. Polar marine ecosystems are ideal places to study such complex interactions due to stark seasonal shifts between heterotrophic and autotrophic communities, and temperatures that may limit microbial processing of organic matter. The research will test the following hypotheses: 1) heterotrophic bacteria compete with macrofauna for food; 2) as phytoplankton populations decline macrofauna increasingly consume microbial biomass to sustain their populations; and 3) in the absence of seasonal photosynthetic inputs, macrofaunal biodiversity will decrease unless supplied with microbially derived nutrition. Observational and empirical studies will test these hypotheses at McMurdo Station, Antarctica, where a high-abundance macro-infaunal community is adapted to this boom-and-bust cycle of productivity. The investigator will mentor undergraduates from a predominantly minority-serving institution, in the fields of invertebrate taxonomy and biogeochemistry. The general public and young scientists will be engaged through lectures at local K-12 venues and launch of an interactive website. The results will better inform scientists and managers about the effects of climate change on polar ecosystems and the mechanisms of changing productivity patterns on global biodiversity.</p>
McMurdo Benthos
largerWorkCitation
project
eng; USA
oceans
166.639
166.639
-77.846
-77.846
2017-10-11
Ross Sea, 78 S 167 E
0
BCO-DMO catalogue of parameters from Fatty acid data from infauna in sediment cores at McMurdo Station, Antarctica in September, November and February of 2012 and 2013 (McMurdo Benthos project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/716677.rdf
Name: Taxa
Units: unitless
Description: <p>Species that had its fatty acid profile measured</p>
http://lod.bco-dmo.org/id/dataset-parameter/716678.rdf
Name: Sample_Identifier
Units: unitless
Description: <p>unique identifier for the individual that was analyzed</p>
http://lod.bco-dmo.org/id/dataset-parameter/716679.rdf
Name: Sample_date
Units: unitless
Description: <p>Date the sample was collected in format yyyy-mm-dd</p>
http://lod.bco-dmo.org/id/dataset-parameter/716680.rdf
Name: Core_Replicate
Units: unitless
Description: <p>The sample identifier for the sediment core that the individual was collected from</p>
http://lod.bco-dmo.org/id/dataset-parameter/716681.rdf
Name: Fatty_Acid_ID
Units: unitless
Description: <p>Identity of the fatty acid using lipid number (e.g. C16:2) which includes number of carbons in the fatty acid chain : number of double bonds in the in fatty acid chain and, where indicated, (n-x) notation indicates the location of the first double bond from the terminal end of the Fatty Acid Chain. Unidentified fatty acids (e.g. Unident. FA # 21) were assigned numeric identifiers consistent amongst all samples. See processing description section for more details.</p>
http://lod.bco-dmo.org/id/dataset-parameter/716682.rdf
Name: Fatty_Acid_Concentration
Units: percent
Description: <p>Percent of total fatty acids recovered from that individual sample.</p>
http://lod.bco-dmo.org/id/dataset-parameter/716683.rdf
Name: lat
Units: decimal degrees
Description: <p>Latitude of sample; north is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/716684.rdf
Name: lon
Units: decimal degrees
Description: <p>Longitude of sample; west is negative</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
25585
https://datadocs.bco-dmo.org/file/0AAMNY3tM6BK3m/FattyAcid.csv
FattyAcid.csv
Primary data file for dataset ID 716455
download
https://osprey.bco-dmo.org/dataset/716455/data/download
download
onLine
dataset
<p><strong>Methodology:&nbsp;</strong><br />
Sample collection and field treatment: Sediment cores were collected at the “Jetty” dive site location in September, November and February 2012-2013.&nbsp; These were sieved on a 300-micron sieve to collect macrofauna and members of the abundant species Spiophanes tcherniai (a spionid polychaete) and Edwarsia spp.&nbsp; (a sand anemone) were picked under a dissecting microscope and placed in filtered seawater overnight to allow gut evacuation. At this point, individuals were frozen separately at -80 C prior to lipid extraction.</p>
<p><strong>Sampling and analytical procedures:</strong><br />
Samples were freeze-dried and their lipids extracted using one-step extraction-transesterification procedure of Lewis et al. (2000) as used in Thurber (2014). In brief, individuals were placed in pre-cleaned and extracted sample vials, heated to 90 deg. C for one hour in a solution of methanol, chloroform, and hydrochloric acid (10:1:1 by volume) before cooling and water added to break the phase.&nbsp; Fatty acid methyl esters (FAMEs) were extracted in sequential addition of hexane:chloroform (4:1) and water further removed through the addition of sodium sulfate.&nbsp; The FAMEs were then ready for quantification on a GC-FID.&nbsp;</p>
<p>Isotopic analysis was measured on a Thermo 1310 gas chromatograph with a Flame Ionization detector using a TR-WaxMS column (30mx 0.32mm x 0.25 um).&nbsp; Peak identification was by comparison to known standards (for example the Supulco 32 FAME mixture).</p>
Specified by the Principal Investigator(s)
<p>Peak identity and area were manually checked and we report concentration as a percentage of total FAMEs for those that make up more than 1 percent of the total integrated area of the chromatogram.&nbsp; We have been conservative in peak identification, with multiple unidentified FAs indicated, however, these are comparable across the samples (i.e. Unident. FA # 2 is the same in all individuals analyzed).</p>
<p>BCO-DMO Data Manager Processing Notes:<br />
* added a conventional header with dataset name, PI name, version date<br />
* modified parameter names to conform with BCO-DMO naming conventions<br />
* parameter names modified for clarity: Fatty_Acid -&gt; Fatty_Acid_ID ; Concentration -&gt; Fatty_Acid_Concentration ;&nbsp; Replicate -&gt; Core_Replicate<br />
* updated coordinates in data for hutt&nbsp;point sample site based on correspondence with data contributor.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Thermo 1310 gas chromatograph
Thermo 1310 gas chromatograph
PI Supplied Instrument Name: Thermo 1310 gas chromatograph PI Supplied Instrument Description:Isotopic analysis was measured on a Thermo 1310 gas chromatograph with a Flame Ionization detector using a TR-WaxMS column (30mx 0.32mm x 0.25 um). Instrument Name: Gas Chromatograph Instrument Short Name:Gas Chromatograph Instrument Description: Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB02/
Deployment: Thurber_Hutt_Point_2012
Thurber_Hutt_Point_2012
Ross Island
island
Thurber_Hutt_Point_2012
Andrew Thurber
Oregon State University
Ross Island
island