http://lod.bco-dmo.org/id/dataset/716462
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2017-10-09
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Observational and experimental stable isotope data including stable isotopes from infauna collected in sediment cores at McMurdo Station, Antarctica in September, November and February of 2012 and 2013 (McMurdo Benthos project)
2017-10-11
publication
2017-10-11
revision
BCO-DMO Linked Data URI
2017-10-11
creation
http://lod.bco-dmo.org/id/dataset/716462
Andrew Thurber
Oregon State University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Thurber, A. (2017) Observational and experimental stable isotope data including stable isotopes from infauna collected in sediment cores at McMurdo Station, Antarctica in September, November and February of 2012 and 2013 (McMurdo Benthos project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Version Date 2017-10-11 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/716462 [access date]
Stable isotopic composition of McMurdo Benthos Dataset Description: <p>This dataset contains&nbsp;observational and experimental stable isotope data.&nbsp; Observational stable isotope data from infauna&nbsp;in sediment cores was collected at the “Jetty” dive site, Hutt Point, McMurdo Station, Antarctica.&nbsp;Seasonal sampling took place in September, November and February 2012-2013.&nbsp; This dataset also includes stable isotope data from isotopic labeling and mesocosm experiments.</p> Methods and Sampling: <p><strong>Methodology:&nbsp;</strong><br />
Observational component: Sediment cores were collected at the “Jetty” dive site location in September, November and February 2012-2013.&nbsp; These were sieved on a 300-micron sieve to collect macrofauna and sorted to putative species under a dissecting microscope, allowed to evacuate their guts overnight in 0.6 micron filtered seawater and then frozen at -80 C for later analysis.&nbsp; All samples for these experiments were from the “Spiophanes beds” that are a dense infaunal community that is present at this location.</p>
<p><strong>Manipulative experiment:&nbsp;</strong><br />
Sediment cores were collected in mid September 2012 from four areas with dense Spiophanes beds and placed in the seawater tables at the Crary Lab at McMurdo station.&nbsp; Treatments included with or without antibiotics (100 microgram l-1 Kanamycin) and with three different levels of food (freeze dried Phaeocystis antarctica) at levels of 0.04 grams to 0.17 grams representing different food stresses.&nbsp; Replicate cores were sacrificed at time intervals (between 0.5 and 6 weeks) and kept in the dark.&nbsp; At the termination of each core it was sieved and treated as above.</p>
<p><strong>Pulse-chase experiment:</strong><br />
An additional selection of cores were taken in mid October and placed in the dark. Phaeocystis antarctica that had been grown on H13CO3 were added to the cores either with antibiotics or without and with 15NH4 to identify if antibiotics inhibited the uptake of NH4 and the routing of carbon through the macrofaunal community. At the termination of the experiment, all cores were sieved and fauna sorted as in the above observational and manipulative experiments.&nbsp;&nbsp;<br />
&nbsp;<br />
<strong>Sampling and analytical procedures:</strong><br />
All in dried overnight at 60 degrees, acidified using 1 M HCl, and their stable isotopic analysis measured at Washington State University on approximately 0.8 mg of tissue. Pulse chase and natural abundance samples were run separately.&nbsp;</p>
<p>Isotopic analysis was measured on a Eurovector elemental analyzer interfaced with a continuous flow Micromass Isoprime isotope ratio mass spectrometer (irms) at Washington State University.</p>
Funding provided by NSF Office of Polar Programs (formerly NSF PLR) (NSF OPP) Award Number: OPP-1103428 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1103428
onGoing
Andrew Thurber
Oregon State University
541-737-4500
athurber@ucsb.edu
pointOfContact
asNeeded
Unknown
Taxa
Sample_date
Core_Replicate
Experiment
Time_point
Algae
Antibiotics
Isotopic_Label
delta_13C
delta_N15
lat
lon
Eurovector elemental analyzer
continuous flow Micromass Isoprime isotope ratio mass spectrometer (irms)
theme
None, User defined
No BCO-DMO term
date
core id
experiment type
time_elapsed
treatment
d13C
d15N
latitude
longitude
featureType
BCO-DMO Standard Parameters
Elemental Analyzer
Mass Spectrometer
instrument
BCO-DMO Standard Instruments
Thurber_Hutt_Point_2012
service
Deployment Activity
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Microbe - Metazoan Interactions in an Antarctic Infaunal Community
https://osprey.bco-dmo.org/project/716214
Microbe - Metazoan Interactions in an Antarctic Infaunal Community
<p>The biota of the world's seafloor is fueled by bursts of seasonal primary production. For food-limited sediment communities to persist, a balance must exist between metazoan consumption of and competition with bacteria, a balance which likely changes through the seasons. Polar marine ecosystems are ideal places to study such complex interactions due to stark seasonal shifts between heterotrophic and autotrophic communities, and temperatures that may limit microbial processing of organic matter. The research will test the following hypotheses: 1) heterotrophic bacteria compete with macrofauna for food; 2) as phytoplankton populations decline macrofauna increasingly consume microbial biomass to sustain their populations; and 3) in the absence of seasonal photosynthetic inputs, macrofaunal biodiversity will decrease unless supplied with microbially derived nutrition. Observational and empirical studies will test these hypotheses at McMurdo Station, Antarctica, where a high-abundance macro-infaunal community is adapted to this boom-and-bust cycle of productivity. The investigator will mentor undergraduates from a predominantly minority-serving institution, in the fields of invertebrate taxonomy and biogeochemistry. The general public and young scientists will be engaged through lectures at local K-12 venues and launch of an interactive website. The results will better inform scientists and managers about the effects of climate change on polar ecosystems and the mechanisms of changing productivity patterns on global biodiversity.</p>
McMurdo Benthos
largerWorkCitation
project
eng; USA
oceans
166.639
166.639
-77.846
-77.846
2017-10-11
Ross Sea, 78 S 167 E
0
BCO-DMO catalogue of parameters from Observational and experimental stable isotope data including stable isotopes from infauna collected in sediment cores at McMurdo Station, Antarctica in September, November and February of 2012 and 2013 (McMurdo Benthos project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/716704.rdf
Name: Taxa
Units: unitless
Description: <p>Species that had its fatty acid profile measured</p>
http://lod.bco-dmo.org/id/dataset-parameter/716705.rdf
Name: Sample_date
Units: unitless
Description: <p>Date which the sample was collected from the environment.</p>
http://lod.bco-dmo.org/id/dataset-parameter/716706.rdf
Name: Core_Replicate
Units: unitless
Description: <p>The sample identifier for the sediment core that the individual was collected from</p>
http://lod.bco-dmo.org/id/dataset-parameter/716707.rdf
Name: Experiment
Units: unitless
Description: <p>Either Observational (direct sampling from the environment to look at seasonality); Mesocosm (manipulated as described below); or Pulse Chase (isotopic labelling experiment). All are described below.</p>
http://lod.bco-dmo.org/id/dataset-parameter/716708.rdf
Name: Time_point
Units: unitless
Description: <p>Relevant only to mesocosm experiments. This is how long the cores were exposed to their treatment before being sacrificed.</p>
http://lod.bco-dmo.org/id/dataset-parameter/716709.rdf
Name: Algae
Units: grams (g)
Description: <p>Amount of Phaeocystis antarctica added to the core; grams rounded to nearest 0.01 gram.</p>
http://lod.bco-dmo.org/id/dataset-parameter/716710.rdf
Name: Antibiotics
Units: unitless
Description: <p>Experimental treatment description; "no" indicates no antibiotics were added and "yes" indicates that cores were exposed to 100 microgram l-1 Kanamycin sulfate at the beginning of the experiment. n/a indicates not relevant for observational cores.</p>
http://lod.bco-dmo.org/id/dataset-parameter/716711.rdf
Name: Isotopic_Label
Units: unitless
Description: <p>Experimental treatment description; no indicates there was no label added and yes means that carbon isotopically labeled Phaeocystis Antarctica was added with 15N labeled ammonium.</p>
http://lod.bco-dmo.org/id/dataset-parameter/716712.rdf
Name: delta_13C
Units: permil (0/00)
Description: <p>Stable carbon isotopic composition of the individual following the per mil standard in reference to a universal standard (pee dee belemnite) i.e. d13C.</p>
http://lod.bco-dmo.org/id/dataset-parameter/716713.rdf
Name: delta_N15
Units: permil (0/00)
Description: <p>delta N15 Stable nitrogen isotopic composition of the individual following the per mil standard in reference to a atmospheric nitrogen gas i.e. d15N.</p>
http://lod.bco-dmo.org/id/dataset-parameter/716714.rdf
Name: lat
Units: decimal degrees
Description: <p>Latitude of sample; north is positive</p>
http://lod.bco-dmo.org/id/dataset-parameter/716715.rdf
Name: lon
Units: decimal degrees
Description: <p>Longitude of sample; west is negative</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
73246
https://datadocs.bco-dmo.org/file/l880lxkukn62o9/Isotopes.csv
Isotopes.csv
Primary data file for dataset ID 716462
download
https://osprey.bco-dmo.org/dataset/716462/data/download
download
onLine
dataset
<p><strong>Methodology:&nbsp;</strong><br />
Observational component: Sediment cores were collected at the “Jetty” dive site location in September, November and February 2012-2013.&nbsp; These were sieved on a 300-micron sieve to collect macrofauna and sorted to putative species under a dissecting microscope, allowed to evacuate their guts overnight in 0.6 micron filtered seawater and then frozen at -80 C for later analysis.&nbsp; All samples for these experiments were from the “Spiophanes beds” that are a dense infaunal community that is present at this location.</p>
<p><strong>Manipulative experiment:&nbsp;</strong><br />
Sediment cores were collected in mid September 2012 from four areas with dense Spiophanes beds and placed in the seawater tables at the Crary Lab at McMurdo station.&nbsp; Treatments included with or without antibiotics (100 microgram l-1 Kanamycin) and with three different levels of food (freeze dried Phaeocystis antarctica) at levels of 0.04 grams to 0.17 grams representing different food stresses.&nbsp; Replicate cores were sacrificed at time intervals (between 0.5 and 6 weeks) and kept in the dark.&nbsp; At the termination of each core it was sieved and treated as above.</p>
<p><strong>Pulse-chase experiment:</strong><br />
An additional selection of cores were taken in mid October and placed in the dark. Phaeocystis antarctica that had been grown on H13CO3 were added to the cores either with antibiotics or without and with 15NH4 to identify if antibiotics inhibited the uptake of NH4 and the routing of carbon through the macrofaunal community. At the termination of the experiment, all cores were sieved and fauna sorted as in the above observational and manipulative experiments.&nbsp;&nbsp;<br />
&nbsp;<br />
<strong>Sampling and analytical procedures:</strong><br />
All in dried overnight at 60 degrees, acidified using 1 M HCl, and their stable isotopic analysis measured at Washington State University on approximately 0.8 mg of tissue. Pulse chase and natural abundance samples were run separately.&nbsp;</p>
<p>Isotopic analysis was measured on a Eurovector elemental analyzer interfaced with a continuous flow Micromass Isoprime isotope ratio mass spectrometer (irms) at Washington State University.</p>
Specified by the Principal Investigator(s)
<p>We are including raw data after eliminating data whose N and C peak response were insufficient for reliable isotopic data as they fell outside of the linear portion of the standard curve.</p>
<p>BCO-DMO Data Manager Processing Notes:<br />
* added a conventional header with dataset name, PI name, version date<br />
* modified parameter names to conform with BCO-DMO naming conventions<br />
* parameter name modified for clarity: Concentration -&gt; Fatty_Acid_Concentration<br />
* updated coordinates in data for hutt point sample site based on correspondence with data contributor.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Eurovector elemental analyzer
Eurovector elemental analyzer
PI Supplied Instrument Name: Eurovector elemental analyzer PI Supplied Instrument Description:Isotopic analysis was measured on a Eurovector elemental analyzer interfaced with a continuous flow Micromass Isoprime isotope ratio mass spectrometer (irms) at Washington State University. Instrument Name: Elemental Analyzer Instrument Short Name: Instrument Description: Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/
continuous flow Micromass Isoprime isotope ratio mass spectrometer (irms)
continuous flow Micromass Isoprime isotope ratio mass spectrometer (irms)
PI Supplied Instrument Name: continuous flow Micromass Isoprime isotope ratio mass spectrometer (irms) PI Supplied Instrument Description:Isotopic analysis was measured on a Eurovector elemental analyzer interfaced with a continuous flow Micromass Isoprime isotope ratio mass spectrometer (irms) at Washington State University. Instrument Name: Mass Spectrometer Instrument Short Name:Mass Spec Instrument Description: General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Deployment: Thurber_Hutt_Point_2012
Thurber_Hutt_Point_2012
Ross Island
island
Thurber_Hutt_Point_2012
Andrew Thurber
Oregon State University
Ross Island
island